| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,004,746 | C→A | 6.9% | intergenic (+89/‑22) | ycbF → / → pyrD | putative periplasmic pilin chaperone/dihydro‑orotate oxidase, FMN‑linked |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,004,746 | 0 | C | A | 6.9% | 325.2 / 9.2 | 98 | intergenic (+89/‑22) | ycbF/pyrD | putative periplasmic pilin chaperone/dihydro‑orotate oxidase, FMN‑linked |
| Reads supporting (aligned to +/- strand): ref base C (47/43); new base A (5/3); total (52/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01 | |||||||||||
ATTAACCTGATTCAGGTGAG‑TGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGATCGATCGAGA > NC_000913/1004481‑1005017 | aTTACCCTGGTTC‑GGGGAGTTGTAAAGTGAAAAGCGTTAAGTAAGTGGGGAGTTAGGCCGCCTAAAAGCCAACGTACTACAAGCTGTAGTCAGGCAATGGCACCGTGTCATGTCGCCTGGCGGGCGCTAAATGATTACGGCCGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTGTTGCCGCAGGTCGATTCCCTTTTGATCCGAACT‑CGCACATAATACGCCCCCGGTTTGAGCACAGGGa < 4:458479/273‑1 (MQ=255) tAACCTGATTCAGGTGAG‑TGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAAACTGCCCTGAAACAGGGTCGGAAAACGTTTGCGTTTTTTTTTGCGCAGGTTAATTCCCTTTTGGTCCGAACT‑CGCACAAAATACGCCCCCGGTTTGCAAACCGGGCAt > 2:458484/1‑273 (MQ=255) caagTGAG‑TGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTc < 2:140351/272‑1 (MQ=255) cAGGGGGG‑TGTAAATGGTAAAGCGGTAAGTAATGTGGGAGTTGTGCCGCCGAAAAGCCAGCGTCAGGAGAGCTGGTGCCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGGGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTc < 4:164227/275‑1 (MQ=255) gtGGTAAAGCGTGAAGAGATGGGGGGGTNNNGCGGACGAAAAGCGAGGGTAAGACAAGCGGGGGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGGGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTAcccc < 3:149997/273‑1 (MQ=255) tAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCTGGAATCCAGGAGAGTTCCTGTACTACCCCTTCTTTCGTaa > 1:149997/1‑273 (MQ=255) gAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTTCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCCCATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGGGGGTTTATATACTACCCATTCGTTCCTCAATCCCTTTTctatc > 1:17792/1‑271 (MQ=255) gtgCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGGAAAGAAGGAGCAAAAACTGCCCTTAAAACGGGTCGGGAAACGTTTGCCGTTTTTTTGCCGCAGGGCAATTCCCTTTTGGTCCGAACTACCGACCCTA‑ACTCCCCCTGTTTGCAAACCCGGAATCCCGGAGATTTAATGTACTCCCCCTTCTCTCTTAAACCCCTGTTACactccaaa > 4:161738/1‑268 (MQ=255) ccTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGgaga > 2:402658/1‑223 (MQ=255) aaaGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTc < 4:402653/223‑1 (MQ=255) aaggccaatgCACCGGTTCATGTGGCCTGGCGGGAGATAAAGGAGTACGGCGGGTGGAGTGAAAAGAAGGAGAAAAAACTGCCCTGAAACAGGTTCCGAAAACGTTTGCGTTTTTTTCGCCGCAGTTCAATTCCCTTGTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCGTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTa < 4:192900/266‑1 (MQ=255) gCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACg < 1:123311/274‑1 (MQ=255) gCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGc < 4:38569/275‑1 (MQ=255) ccTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGGTTTTTTTGCCGCAGGTCAAATCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCCCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAgctgtctcttat > 2:427434/1‑263 (MQ=255) ggcgATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCgg < 6:210550/149‑1 (MQ=255) gATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCCGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGAACACCGGGaa > 7:182615/1‑149 (MQ=255) aTGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAg > 5:437815/1‑149 (MQ=255) aTGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTGTGCAAACCgggggc < 7:212555/145‑4 (MQ=255) aTGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTGTGCAAACCgggggc > 8:212555/1‑142 (MQ=255) tGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAgg > 7:31861/1‑149 (MQ=255) tACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGagag > 2:262219/1‑149 (MQ=255) gcggGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCa < 4:262215/149‑1 (MQ=255) ggTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGGAAACGGTTGCGTTTTTTTTGACGCAGGTCAATTCCCGTTTGGTCCGAACT‑CGCACATAATAAGCCCCCGGTTTGCACACCGGGAATCCAGGAAGATTCATTTACTACCCCCTGCTTCGTAAAACCCTTTTCCAGATCGAACCAGTGCGCGCCCCTGAGTTTACTTTTCAGAAATTACTCCGTATTACAGGAAACCCGTGTGAAGCCCTGGGGCGCGATAAAGTGccc > 4:124699/1‑273 (MQ=255) gTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTGTGCAAACCggggg < 8:412477/131‑3 (MQ=255) gTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTGTGCAAACCggggg > 7:412477/1‑129 (MQ=255) ttGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGtac < 7:196728/149‑1 (MQ=255) ttGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGtac < 7:198101/149‑1 (MQ=255) gAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGa < 2:124700/274‑1 (MQ=255) aGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACACAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGaa < 2:161740/274‑1 (MQ=255) gTGCAAGGAAGGAGCAAAAGCTGCCCTGAAACAGGTTCGGAAAACGTTGGCGTTTTTTTTGCCGCAAGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGAGAATCCCGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGaaa < 2:331164/274‑1 (MQ=255) cAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCt > 6:110962/1‑149 (MQ=255) cggagGCGCAAAATCTTCCTTGCAACAGGTTTGGAAGACGTCTCCGATTTTTTTACTGCACGTCAATTCCCTTTTCGTCTGAACA‑CCTAAATATTACGCCGCCGTTTTGCACATCGGGAATCCAGGTGGGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGCGCGCGCTCATTAGTTTACTTTTCAGCAATTACGCCGTATTACCGTAACGCCGTTTGAAGACGTGGTTCGGCAGAAAGTGCCTGCGaaaacgttta > 4:34109/4‑268 (MQ=255) agaagGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCg > 2:91183/1‑232 (MQ=255) agGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTaa > 2:314458/1‑153 (MQ=255) gAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCACCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGGCCGATCCAGCGCGCGCCCCTGACTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGcc > 4:23216/1‑226 (MQ=255) gCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGcc < 4:314454/153‑1 (MQ=255) gCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGAACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCa < 2:34109/274‑1 (MQ=255) cAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTaaa > 8:432544/1‑149 (MQ=255) aaaaTCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGttt < 2:23216/226‑1 (MQ=255) tCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCt < 8:182615/149‑1 (MQ=255) gCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCCGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTTCGGAAGAACATGCCTGCGAACCCGGTTAACTGCATGGGCCTGa > 4:346444/1‑274 (MQ=255) tGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGgaga < 6:184917/109‑1 (MQ=255) tGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGgaga > 5:184917/1‑109 (MQ=255) gAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTc < 5:261993/145‑1 (MQ=255) gAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTc > 6:261993/1‑145 (MQ=255) ccggTTCGGAAACCGTGTGCCTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCAGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTGCGCCGGATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTaaaaa < 2:490765/273‑1 (MQ=255) ggTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATc < 5:110962/149‑1 (MQ=255) ttCGGAAAACGTTTGCG‑TTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTc > 5:55092/1‑142 (MQ=255) ttCGGAAAACGTTTGCG‑TTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTc < 6:55092/142‑1 (MQ=255) ggAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGaa < 5:419353/88‑1 (MQ=255) ggAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGaa > 6:419353/1‑88 (MQ=255) gAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATGCGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAgc > 5:1905/1‑149 (MQ=255) gAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAgc < 5:10091/149‑1 (MQ=255) tGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGACCTTTTCCAGCTCGCTCCAGAGCGCGCTAATGAGTTTACTTTTCAGCAATTACGCCTTATTACCGGAACGCCGTTTGAATCACTGGTTAGGCAGCACGTGCCTGCGAACCCTGTTAACTGCATGGGACTGCCGTTTAACAATCCGCTTGGTATTGCAg > 1:17254/1‑275 (MQ=255) gTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGc < 6:313930/125‑1 (MQ=255) gTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGc > 5:313930/1‑125 (MQ=255) gTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGt < 8:31861/149‑1 (MQ=255) gTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGt > 8:226795/1‑149 (MQ=255) ttttttttGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGtt > 7:124255/1‑149 (MQ=255) gCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGTATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTc < 8:59009/149‑1 (MQ=255) ccGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCa < 8:358407/149‑1 (MQ=255) aaTTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACg > 8:42493/1‑149 (MQ=255) aTTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGc > 7:180433/1‑149 (MQ=255) aTTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGc > 7:94757/1‑149 (MQ=255) ttCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCt > 4:332735/1‑96 (MQ=255) ttCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGcc > 8:63888/1‑149 (MQ=255) ttCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGcc > 6:382501/1‑149 (MQ=255) ttCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGcc > 6:468233/1‑149 (MQ=255) ccTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTa < 6:437815/149‑1 (MQ=255) cTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTc < 2:332739/96‑1 (MQ=255) cTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTAt > 7:73163/1‑149 (MQ=255) tGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTTCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGAc > 2:175990/1‑275 (MQ=255) gTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAAc > 3:119276/1‑201 (MQ=255) gAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCa < 1:119276/201‑1 (MQ=255) aagagacagCCCCCGGTTTGCACACCGGTAATCCTGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGAt < 2:58008/266‑1 (MQ=255) aataCGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTAc < 6:347004/120‑1 (MQ=255) aataCGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTAc > 5:347004/1‑120 (MQ=255) aataCGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCa < 8:364664/149‑1 (MQ=255) taCGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGaa > 6:302042/1‑144 (MQ=255) taCGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGaa < 5:302042/144‑1 (MQ=255) taCGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACt > 6:121672/1‑149 (MQ=255) taCGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACt > 5:250939/1‑149 (MQ=255) ccccGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGc > 5:214650/1‑149 (MQ=255) cccGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCAc > 1:111090/1‑142 (MQ=255) cccGGTTTGCACACCGGGAACCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCAt < 5:283280/90‑1 (MQ=255) cccGGTTTGCACACCGGGAACCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCAt > 6:283280/1‑90 (MQ=255) ccGGTGTGCAAACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAgcgcgc < 7:36946/85‑1 (MQ=255) ccGGTGTGCAAACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAgcgcgc > 8:36946/1‑85 (MQ=255) cGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCTTCAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCGTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGCGTGCATTGACGCGTTAGGCGCGATGGGATGTGgagcct > 4:14889/1‑271 (MQ=255) cGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATTAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGTGATGGGATTTGgatcga > 2:310957/1‑275 (MQ=255) cGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGcc > 4:427066/1‑113 (MQ=255) cGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCt > 2:336175/1‑160 (MQ=255) cGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAACCCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGTTAAAGACGGGGAGTGCATTGACGCGTTGGgcc > 4:220390/1‑256 (MQ=255) gTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGt < 3:111090/142‑1 (MQ=255) gTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGgatcgatc < 2:24846/275‑1 (MQ=255) ttGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTAt < 2:427071/113‑1 (MQ=255) ttGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGa < 4:336171/160‑1 (MQ=255) ttGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGAt < 2:220394/257‑1 (MQ=255) cacaCCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGATCGATCgaga > 2:177546/1‑274 (MQ=255) cacaCCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCGCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGGCGTTTGAAGCACTGGTTAGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCTAGGGCCTGACGTTTCAAAATCGGATTGGCTTGGCAGCCGGTCTTGAGAAAGCAGGGGAGTGAGTTGACGCGTTAGACGCGCAGGGGTTTGgatctgtctctt > 4:58007/1‑266 (MQ=255) | ATTAACCTGATTCAGGTGAG‑TGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACT‑CGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGATCGATCGAGA > NC_000913/1004481‑1005017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |