| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,339,813 | T→C | 8.2% | K85E (AAA→GAA) | mlaF ← | ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,339,813 | 0 | T | C | 8.2% | 229.5 / 8.8 | 73 | K85E (AAA→GAA) | mlaF | ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (41/26); new base C (2/4); total (43/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
GCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGAT > NC_000913/3339666‑3339960 | gCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTg > 2:16657/1‑149 (MQ=255) gCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGt < 1:16657/149‑1 (MQ=255) ttCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑gcg > 1:232479/1‑149 (MQ=255) ttCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑gcg > 3:416028/1‑149 (MQ=255) atcatcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGtata < 3:231010/140‑1 (MQ=255) atcatcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGtata > 4:231010/1‑140 (MQ=255) tcatcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTGATCCGTTTGCGCACTGTATACAG‑GCGCGaa > 1:309038/1‑149 (MQ=255) tcatcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGaa > 3:286783/1‑149 (MQ=255) catcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAAc < 4:125995/149‑1 (MQ=255) atcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACg > 4:212454/1‑149 (MQ=255) atcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACg > 4:332774/1‑149 (MQ=255) tcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACgg < 1:34897/149‑2 (MQ=255) aCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACgaga < 2:289526/149‑1 (MQ=255) ccGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACggat > 2:72679/1‑145 (MQ=255) ccGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACggat < 1:72679/148‑4 (MQ=255) tACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTgcg < 3:158948/121‑1 (MQ=255) tACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTgcg > 4:158948/1‑121 (MQ=255) aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAat > 4:50186/1‑149 (MQ=255) aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAat > 1:135469/1‑149 (MQ=255) aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAat > 2:186641/1‑149 (MQ=255) ggCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtctc < 4:416028/149‑1 (MQ=255) gcgcGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCa < 4:372736/149‑1 (MQ=255) gTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGC‑CTGtat < 1:272493/104‑1 (MQ=255) gTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGC‑CTGtat > 2:272493/1‑104 (MQ=255) gTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATGACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGAt > 4:60195/1‑149 (MQ=255) aGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATc < 3:332774/149‑1 (MQ=255) ggATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTgg > 1:348281/1‑149 (MQ=255) aTAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTg < 1:186641/149‑1 (MQ=255) ggCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATGACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGa < 3:60195/149‑1 (MQ=255) gCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAt > 2:72181/1‑149 (MQ=255) gCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAt > 3:33318/1‑149 (MQ=255) gCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAt < 2:56557/149‑1 (MQ=255) cGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAtt < 3:50186/149‑1 (MQ=255) gACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtc < 2:30196/109‑1 (MQ=255) gACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtc > 1:30196/1‑109 (MQ=255) gTTGTCAAATACGTTCATATCATTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACg < 3:143844/87‑1 (MQ=25) gTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACg > 4:143844/1‑87 (MQ=37) gTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGc < 3:212454/149‑1 (MQ=255) tCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACga > 3:80836/1‑84 (MQ=255) tCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACga < 4:80836/84‑1 (MQ=255) aaaTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGt < 1:402135/133‑1 (MQ=255) aaaTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGt > 2:402135/1‑133 (MQ=255) aaaTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCaa > 1:456740/1‑149 (MQ=255) aTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATc > 1:158925/1‑149 (MQ=255) tACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAg > 3:379309/1‑111 (MQ=255) tACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAg < 4:379309/111‑1 (MQ=255) ttCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACg > 4:300465/1‑149 (MQ=255) tatCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGt > 1:384742/1‑149 (MQ=255) atCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTa > 4:108497/1‑149 (MQ=255) tCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAg > 4:267472/1‑149 (MQ=255) aGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTTACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcg > 2:429885/1‑149 (MQ=255) gTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgt > 4:273549/1‑149 (MQ=255) tGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtc > 3:17042/1‑149 (MQ=255) gAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtcg < 2:232479/149‑1 (MQ=255) aacaacGCCCCGGACCGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtcgt > 4:125793/1‑149 (MQ=255) cccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa > 1:380537/1‑86 (MQ=255) cccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa < 2:380537/86‑1 (MQ=255) ccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGAt < 3:108497/149‑1 (MQ=255) tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGc > 4:229791/1‑145 (MQ=255) tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGc < 1:453857/145‑1 (MQ=255) tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGc < 3:229791/145‑1 (MQ=255) tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGc > 2:453857/1‑145 (MQ=255) tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGa > 4:430013/1‑149 (MQ=255) aaaTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtc > 1:51822/1‑64 (MQ=255) aaaTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtc < 2:51822/64‑1 (MQ=255) aaaTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGa > 2:281077/1‑122 (MQ=255) aaaTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGa < 1:281077/122‑1 (MQ=255) tAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGcc > 4:84713/1‑149 (MQ=255) aaCATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGccc > 2:265220/1‑149 (MQ=255) aTCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTg > 3:61186/1‑92 (MQ=255) aTCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTg < 4:61186/92‑1 (MQ=255) aTCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGc > 1:307493/1‑149 (MQ=255) gTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTTACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTg < 1:429885/149‑1 (MQ=255) ttGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGAt < 3:84713/149‑1 (MQ=255) | GCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGAT > NC_000913/3339666‑3339960 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |