Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,261,359 T→A 9.8% intergenic (+53/‑310) yjbM → / → dusA uncharacterized protein/tRNA‑dihydrouridine synthase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,261,3590TA 9.8% 161.9 / 8.5 51intergenic (+53/‑310)yjbM/dusAuncharacterized protein/tRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand):  ref base T (23/23);  new base A (3/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTC  >  NC_000913/4261216‑4261499
                                                                                                                                                |                                                                                                                                            
gTTTGACCCAACCTTAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTg                                                                                                                                         >  3:35578/1‑149 (MQ=255)
gTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTg                                                                                                                                         <  1:80106/149‑1 (MQ=255)
    gACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAAGAACCCAATAAATATTGGGt                                                                                                                                      <  1:363910/149‑1 (MQ=255)
       ccAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCtt                                                                                                                                  <  3:12677/149‑1 (MQ=255)
       ccAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGAACCCAATAAATATTGGGtcc                                                                                                                                    >  1:161157/1‑145 (MQ=255)
       ccAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGAACCCAATAAATATTGGGtcc                                                                                                                                    <  2:161157/147‑3 (MQ=255)
        cAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCttt                                                                                                                                 <  4:204969/149‑1 (MQ=255)
           ccTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCtttttt                                                                                                                              <  1:66832/149‑1 (MQ=255)
            cTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTg                                                                                                                                         >  1:273241/1‑137 (MQ=255)
            cTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTg                                                                                                                                         <  2:273241/137‑1 (MQ=255)
                 aGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTAt                                                                                                                        <  4:102294/149‑1 (MQ=255)
                    aaGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCaa                                                                                                                     <  2:4060/149‑1 (MQ=255)
                         aTTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTa                                                                                                                >  3:209783/1‑149 (MQ=255)
                                tACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATc                                                                                                                       >  3:336552/1‑135 (MQ=255)
                                tACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATc                                                                                                                       <  4:336552/135‑1 (MQ=255)
                                 aCTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAgg                                                                                                        >  3:396509/1‑149 (MQ=255)
                                   ttACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGga                                                                                                      <  2:25366/149‑1 (MQ=255)
                                    gaCAGACACTTATAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCAATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGaag                                                                                                     <  4:232373/148‑1 (MQ=255)
                                      cAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGat                                                                                                   <  4:35578/149‑1 (MQ=255)
                                             ttCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCAc                                                                                            >  4:320360/1‑149 (MQ=255)
                                             ttCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCAc                                                                                            <  1:303950/149‑1 (MQ=255)
                                                           aTGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAAtgtg                                                                              <  3:320360/149‑1 (MQ=255)
                                                             gATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAAtgtgtg                                                                            >  2:216092/1‑149 (MQ=255)
                                                                   ttCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGa                                                                           <  4:228990/144‑1 (MQ=255)
                                                                   ttCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGa                                                                           >  3:228990/1‑144 (MQ=255)
                                                                       gggTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAAtattta                                                                                                                                            >  2:395370/1‑75 (MQ=255)
                                                                       gggTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAAtattta                                                                                                                                            <  1:395370/75‑1 (MQ=255)
                                                                       gggTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTtttatt                                                                  >  3:117580/1‑149 (MQ=255)
                                                                         gTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGa                                                                           <  4:125360/138‑1 (MQ=255)
                                                                         gTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGa                                                                           >  3:125360/1‑138 (MQ=255)
                                                                          ttAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTttta                                                                    <  3:361086/144‑1 (MQ=255)
                                                                          ttAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTttta                                                                    >  4:361086/1‑144 (MQ=255)
                                                                                       ataataTGAGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGa                                                  >  4:40848/1‑149 (MQ=255)
                                                                                              gtgAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGgaga                                                                                                    >  3:249600/1‑92 (MQ=255)
                                                                                              gtgAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGgaga                                                                                                    <  4:249600/92‑1 (MQ=255)
                                                                                                    ttcttcGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTg                                     >  3:63120/1‑149 (MQ=255)
                                                                                                               tATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACaaa                          <  3:40848/149‑1 (MQ=255)
                                                                                                               tATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACaaa                          >  4:14498/1‑149 (MQ=255)
                                                                                                               tATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACaaa                          <  3:14498/149‑1 (MQ=255)
                                                                                                                 tGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGt                        >  1:314738/1‑149 (MQ=255)
                                                                                                                       gCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACgg                  >  1:299589/1‑149 (MQ=255)
                                                                                                                          ttATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGc                                   >  3:44/1‑129 (MQ=255)
                                                                                                                          ttATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGc                                   <  4:44/129‑1 (MQ=255)
                                                                                                                          ttATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCta               >  3:67085/1‑149 (MQ=255)
                                                                                                                              at‑aAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCtaataa           <  4:209783/149‑1 (MQ=255)
                                                                                                                                 aaaaGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGa         <  4:117580/149‑1 (MQ=255)
                                                                                                                                 aaaaGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGa         >  3:83672/1‑149 (MQ=255)
                                                                                                                                      gACCCAATATTTATTGGTTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGattatt    >  3:155746/1‑149 (MQ=255)
                                                                                                                                      aaCCCAATAAATATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGattatt    >  4:92818/2‑149 (MQ=255)
                                                                                                                                       aCCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCtaat             >  4:32982/1‑139 (MQ=255)
                                                                                                                                       aCCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCtaat             <  3:32982/139‑1 (MQ=255)
                                                                                                                                        cccAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTc  >  1:122162/1‑149 (MQ=255)
                                                                                                                                        cccAATAAATATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTc  >  3:55441/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                            
GTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATAT‑AAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTC  >  NC_000913/4261216‑4261499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑