Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,875,521	0	T	C	23.6%	80.0 / 13.4	55	A28A (GCT→GCC)	yidX	putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (25/17);  new base C (13/0);  total (38/17)
Fisher's exact test for biased strand distribution p-value = 5.09e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGCGTTTGCCGTGCGTGTAATGTAGTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCT  >  NC_000913/3875250‑3875790                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                              cgcgTTTGCCGTGCGTGTAATGTAGTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTg                                                                                                                                                                                                                                                                            >  2:388713/1‑275 (MQ=255)                               aCTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGa                                                                                                                                                                                                                                              >  2:31278/1‑275 (MQ=255)                               aCTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGa                                                                                                                                                                                                                                              >  1:390467/1‑275 (MQ=255)                                    tatTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTTTGGTCTCCCATACCGCAAAGGGTAGTTCAGGaaatga                                                                                                                                                                                                                                         >  1:355243/1‑272 (MQ=255)                                         ttgtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGCAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATTTCGTATGCCGTTGTTTCCCTTAACGCAAAAGGTACGTCAGGAAAGTATaaac                                                                                                                                                                                                                                    >  1:525738/1‑274 (MQ=255)                                         ttgtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTCTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATTCTTTCAGGTCGTATCTCGTATGCTCTTGTTTCCCATACCGCAAAGGGAAGTTCATGAAATTATCAAt                                                                                                                                                                                                                                    >  1:137347/1‑275 (MQ=255)                                           gtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATTCGTCGGGCAGAAATCTGACAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCTTTTAAAGGATTAATCCTGCAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCCTACCTCacctggtcgttc                                                                                                                                                                                                                                                  >  1:205360/1‑248 (MQ=255)                                           gtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCTTACCGCAAAGGGGAGTTCAGGAAAGTATCa                                                                                                                                                                                                                                      >  1:599583/1‑271 (MQ=255)                                           gtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATAGCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTTCCATACCGCAAAGGGTCGTTCAGGAAAGTACCAATc                                                                                                                                                                                                                                   >  1:212389/1‑274 (MQ=255)                                             actacAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGc                                                                                                                                                                                                                                 >  2:190382/1‑274 (MQ=255)                                               taaaaTTTAGATCACAAAAAGAACAATGCAGAAAAAAATGACATGCGTCTGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGAGAAAGAAGAGTATGCTTTCAAAGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCg                                                                                                                                                                                                                                  <  3:599583/268‑1 (MQ=255)                                                 cAATTTAGAACACAGAAATAACAATGAATAAAAAAAAAAAATGTGTATGGCAGAATTATGAAAAGGAATAACAGGCACTAAACAACAAGAAAAAAAGATGAAGATTTAAACGGATTAGCTACTCGTTTAAAGGATTAATAGTGAAGTGGAGTTTTAAGTGAATTGTTAAGACGGCCGGT‑TTAATCCCACTGTCGCTGATGATTTCAGGCTGTATCTCGTATGTTTTGGTTTCCCCTACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCa                                                                                                                                                                                                                            <  3:313715/275‑1 (MQ=255)                                                      taaataacaAAAAGAAAGATGCATAAAAAAAAGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAATAGTATGCTTTCAACGGCTTAGCTACTCGTTTCAAGGATTAATCATGAAGTTGAGTTTTAAGGGATTATTTAAGGCTGCTGTT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATCCCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGacac                                                                                                                                                                                                                       <  3:365909/269‑1 (MQ=255)                                                      tAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTTTGCCTTAAAGAAAAGGGTAGTGTAGTAAATTATCAATCGCAGTcgcaca                                                                                                                                                                                                                        >  2:419544/1‑269 (MQ=255)                                                         aTCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGTTTTGCCATACAGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCAt                                                                                                                                                                                                                    >  2:93497/1‑275 (MQ=255)                                                           cagaaaaaGAACAAAGAATAAAAAAATGACATGGTGCGGGCAGAAATATGGAAAGGGATATCAGGCGATAAACAGGAGTGAAAGAAGTGTATGCTTTCAACGGCTTAGCTACTCGTGTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCa                                                                                                                                                                                                                  <  3:11681/272‑1 (MQ=255)                                                                        aTGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCCGGAAAGTACCAATCGCAGTCAGAAACGATCAATGGCATTTCGCa                                                                                                                                                                                                     >  3:110716/1‑275 (MQ=255)                                                                        aTGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCgag                                                                                                                                                                                                     >  2:140686/1‑273 (MQ=255)                                                                           cATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTGGTCTGCCAGAAAGCAAAGGGTGGTTCAGGTAAGTATCAATTGAAGTCAGAAACCATCACTGGGAAATAGCAggg                                                                                                                                                                                                  >  2:487315/1‑274 (MQ=255)                                                                              aaaaaaaTGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTACACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGCCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCAGTATCTCGTATGCTCTGGTTTCCCCTCCCGCAATGGATAGTTCGGGAACGTATCAATCGAATTCCGACACCATCATGGGTCTATCGTAGGCaaa                                                                                                                                                                                               >  3:78852/1‑275 (MQ=255)                                                                              aaaaaaaTGACAGGGGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATAAAGTTGAATGTTAAGAGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCaaa                                                                                                                                                                                               <  2:192600/275‑1 (MQ=255)                                                                                 aaaaTGAAATGCGACGGGCATAAAGTTGTAAAGAAAGATCAGGGGCTAAAGAGACGGAAAAAAAGAGTATCCTTTCAAAGGTTTAGCGAGTAGTTTAAACGATTAATGATGACGTTGAA‑ATGAAAGGTTTTTTGCAGGAATACGATGTAATTTACTCTTGCGCCGATGCTGTCTGCATGTAGCTGGTATGCTCTTTGTTCCGAGACCGCAACGGGTAATTCAGTAATGTATCAATCAGAGTCAGACACCATCACTGGGCTATCGCAGGCAAgaga                                                                                                                                                                                            <  3:390467/275‑1 (MQ=255)                                                                                   aaTGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCCAAAGATa                                                                                                                                                                                          >  1:303196/1‑275 (MQ=255)                                                                                                           tGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCGTCTGCTTTAAAAAAAAAGGTAGTTCAGGAAAGTATAAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATCGTAATGGAACAAAAGGCTATGtt                                                                                                                                                                   >  2:76528/1‑274 (MQ=255)                                                                                                                     tatCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCTTACAGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACAATCACTGGGCTATCGCAGGCAAACGATAGTAATGGAACAACAGGCTATGTTTTTGTAggg                                                                                                                                                          >  2:258485/1‑273 (MQ=255)                                                                                                                     tatCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCTTACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGAAGTCAGACACCCTCACTGTGACCTCGCAGGCAAAAGCTAGTAATGCAACAAAAGGATATGATTTTGtgtgt                                                                                                                                                          >  2:329814/1‑269 (MQ=255)                                                                                                                       tCAGGCGATAAACAGACGCGAAAGCAGAGTATTCTTTCAACGGCTTAACTACTCGTTTAAAAGATTAATCATGAAGTTGAATTTTAAGGTATTTTTTAAGGCTGCCGGT‑TTATTCCCCCTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAGGGGTAGTTCAGGAAAGTATCAATCGCAGTCAAACACCATCACTGTGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAAt                                                                                                                                                      <  3:83109/275‑1 (MQ=255)                                                                                                                        ctggCGCTAAACAGGAGGGGAATAAGGGTATGGTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTATAAGGTATTTTTTAAGGCTGCAGGT‑TTATTCCCGCTGGCGCTGATGCTTTCATGCGGTATCTCGTATGCTCTGGTTGCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATc                                                                                                                                                     <  1:110716/273‑1 (MQ=255)                                                                                                                         agagtcTAAACAGTAGGAAAATATGAGTATACTTTAAACGGCTTAGCTACTCGTTTTAAGGATTAATCTTGAAGTTGAATTTTAATGGATTTTTTAAGGCTGCCGTT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCg                                                                                                                                                    <  4:31278/270‑1 (MQ=255)                                                                                                                           gcgcTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATCCCGCAACGGGTCGTTCAGGCAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCCAGGTTTTTGTAGTGGatgcgc                                                                                                                                                   >  2:275966/1‑269 (MQ=255)                                                                                                                            cgcTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCTTTAACGCAAAGGGTAGTTCAGGAAAGTATCAATTGCAGTCAGACACCATCACTGGGCTATCGTAGGCAAAAGATAGTAATGGAACAAAAGTCTATGTTTTTGTATGGGTATCGc                                                                                                                                                   >  2:41451/1‑273 (MQ=255)                                                                                                                            cgcTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTa                                                                                                                                                                                                          >  1:294140/1‑218 (MQ=255)                                                                                                                                aaaCAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGc                                                                                                                                                                                                      <  3:294140/218‑1 (MQ=255)                                                                                                                                 accgggatgGAAAGAATGGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAAGTTTAAGGTATTTTGTAAAGCTGCCGGT‑TTATTCACACTGGCGCTGATGCTTTCAGGCTGTAACTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGCCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGAt                                                                                                                                              <  3:355243/269‑1 (MQ=255)                                                                                                                                             agaagaGTATGTGTTCAACGGGTTAGCGAATCGTTTAAAGGATGAATCATAAAGTTGAATTTTAAGGGATTTTTTAAGGCGGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTAGCTCGTATGCTCTGGTTTCCCATACCGAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGa                                                                                                                                <  4:140686/275‑1 (MQ=255)                                                                                                                                                    taagCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTAGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAACATAGTCATGGAACAAAAGGCTAGGTTTTTGTAGGGGACTCGCTGGATTACCTTCTCACTGATGGTGcc                                                                                                                         >  3:133307/4‑275 (MQ=255)                                                                                                                                                    tATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAAGGGAACAAAAGGCTATGTTTTGTGAGGGGAATCGGTGGATTTCCTTTTTCCTGATTGTGcc                                                                                                                         >  4:121795/1‑275 (MQ=255)                                                                                                                                                    tATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTTTGCTTGAAAACAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAACGGCTATGTTTTTGTAGGGGAATAGCTGGATTACCTTATCACTGATGTTGcc                                                                                                                         >  2:265315/1‑275 (MQ=255)                                                                                                                                                           acaatggtgtAGCTACACGTTGAAAGGATTAATCATGAGGTTTAATTGTCAGTGATGTTTTAAGGGTGCCGGT‑TTATTACCAATGGGGGTTGTGTTTTAAGGCTGTATATCGTGTGATGTGGTATCTCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTAAGACACCATCACTGGGCTATTTCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGTGGAAGCGCTGGATTACCTTATCACTGATGGTGCCGATGACa                                                                                                                  <  4:258485/266‑1 (MQ=255)                                                                                                                                                            cAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCACAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCt                                                                                                                                                  >  2:31804/1‑242 (MQ=255)                                                                                                                                                                   ttggatACTCGTTTAAAGGATTAATCATGAAGTTGAATGTTAAGGTATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAg                                                                                                          <  1:133307/270‑1 (MQ=255)                                                                                                                                                                   ttAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGTCAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGAAAAAGATCGTAATGGAAAAAAAGGCTATGTTTTTGTAGGGGCATCGCTGGATTACCTTATCACTGATGGTGCCTATGACCTCTTTaa                                                                                                           >  4:331849/1‑274 (MQ=255)                                                                                                                                                                      gatattattttAAAGAGTTTATCATGAAGTTGAATTTTAAGGGATTTGTTAAGGCTGCAGGG‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCCAACCGCAAAGGGTAGTTCAGGAAAGTATCGATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATg                                                                                                       <  3:303196/267‑1 (MQ=255)                                                                                                                                                                        ttatCGTTGAAAGTATTCATCATGAAGTTGTAATTTGAGGGAGTTGTTAAGGCTGCGGGT‑TTATTCCCACCGTGGCTGAAGCTGCCAAGATGTATCACGGATGCTATGCGTTCCCAAAGCGCAAAGGGTAGTTAAGGAAAGTATCAATCGCAATCAGACACCATGATTGGGCTAACGCAGGCAAAAGATAGTAATGGAACAAAAGGGTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGGTAAGTTGCt                                                                                                     <  4:21763/272‑1 (MQ=255)                                                                                                                                                                               ttAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTCGTTTGCCATACCACAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACAGGGCTACCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTATTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGACGACATCGTTAAGATTCTCAATGAt                                                                                              >  1:372457/1‑275 (MQ=255)                                                                                                                                                                               ttAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTCTCCCTTACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGAAAGTAATGGAACAAAAGGCTGTGGTTTTGTAGGGGAATAGCTGGATTACCTTATCACGGATGTTCCCGATGAGAGCGTTCAGCTTTTCAATGAt                                                                                              >  1:352835/1‑275 (MQ=255)                                                                                                                                                                                        tAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCCGGAAAGTATCAATCGCAGTCAGACACTATAACTGGGCTATCGCAGGACAAAGTAAGTACTGGAACAAAAGGATATGTTTTCTTATGGGAATCGCTGGATTTCCATATCACTGATGGAGCAGATGACATCTTGAAGATGATAAATGacacagagatt                                                                                     >  4:110163/1‑265 (MQ=255)                                                                                                                                                                                            cATGAATTTGTATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGTGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAAc                                                                                  <  2:121795/274‑1 (MQ=255)                                                                                                                                                                                                     gAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGACAACAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGCCTCGCTGTATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCAAGCACTTAACCGGCACAata                                                                        >  2:488586/1‑275 (MQ=255)                                                                                                                                                                                                                                                       aTGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTCCTTTACCAAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGATCAATTATCCAGCACTTAACCGGACCCATATTCAAGTTTCCGATGACGCAAGTATTGTGTTAAATACGGGGAAAAAg                         >  2:350342/1‑273 (MQ=255)                                                                                                                                                                                                                                                                     tATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGTTAGTAATGGAACCAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATTACTGATGGTTCCGATGACATCGTTACGATTCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGAGGACCACAGATTTGCTTTACATGCGGGGAAACAGAAATTTACTGGcacca         >  2:372871/1‑273 (MQ=255)                                                                                                                                                                                                                                                                     tATCTCGTATGCCCTGGTCTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATAGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGACGACGCAAGACTTGTTTCAAATGCGGGGAAAAAGAAATTTACAGGcac           >  2:252816/1‑273 (MQ=255)                                                                                                                                                                                                                                                                      aTCTCGTATGCCCTGGTTTCCCATACCGCAAAGGGTAGCTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCATGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATg                                                                                                >  1:428481/1‑187 (MQ=255)                                                                                                                                                                                                                                                                            tATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATAACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGAACAAGCACTTAACCGGAACAATATTAAGGTTGCCGAAGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGACATATACAGGCACaaacacact  >  4:413548/1‑268 (MQ=255)                                                                                                                                                                                                                                                                             aTGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACaa                                                                          >  1:67/1‑202 (MQ=255)                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                              CGCGTTTGCCGTGCGTGTAATGTAGTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGT‑TTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCT  >  NC_000913/3875250‑3875790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑