| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,145,313 | C→A | S683Y (TCC→TAC) | yegE → | putative diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,145,313 | 0 | C | A | 97.9% | 315.7 / NA | 94 | S683Y (TCC→TAC) | yegE | putative diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base A (45/46); minor base G (0/2); total (46/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTG > NC_000913/2145042‑2145580 | gaagaaGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTacc < 1:162777/274‑1 (MQ=255) tAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGAAGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGAAATATCGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAACATGCCG‑GCCACGCTCACATACCGCGCCTccaactctgcctg > 4:314349/1‑263 (MQ=255) gtTCCCCGCCTGACCGGGTTACATATTACTTTTGGCGACAACGGACCATTAAGGGAGAACATTTACTGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCAATGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGc < 1:488545/274‑1 (MQ=255) ccGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGAGCCA‑GCTGAGCTAGAGCGCCTACCATGATGCACTGACGCCtc > 3:139173/1‑274 (MQ=255) cTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCa > 2:138368/1‑274 (MQ=255) cTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGAAATATTGGTTCGGTTTTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCaa > 1:514107/1‑275 (MQ=255) cTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTAGGAGCGGCGGCAGTTATGACGTGCATTACAGTATTACGCCGCTCAGTACTCTGGACGCCAGCAATATTGGTTCGGTTCTGGCGATTCAGGAAGTTACCGAAATACGAAAAACACCGCTCCA‑GCTGCGTATCCGCGCCTAACATCATGCACTCATGTCTCTCGcaca > 3:187073/1‑272 (MQ=255) tGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATCTTGGTTCGGTTCCGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTTCCACC‑GCTGAGCTACAGCACCTACCATGATGCACTGACGCCTCTCCCCCACCCCGcccc > 3:531148/1‑272 (MQ=255) tACTTTTGGCGACAACGGACCATTAATGGAGAACATTCACAGTGCCGACA‑CCTCATG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAATTTCTCTGTACGGCAGCAATCTTGGTTAGGTTCTGGTGATTCAGGACGTTACCTAATCCCGCAAAATGCTCGTCCT‑ACCGAGCTACAGCGCCTCTCTTGACGCACTGAACCATCTCGCCAACCGCACCAGTTTTga > 3:428937/1‑273 (MQ=255) ttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCCCTGACGCATCCCGCCAACCGCGCCAGTTTTGAGaaa > 1:265214/1‑272 (MQ=255) ttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAaacaa < 1:429511/275‑1 (MQ=255) ttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAaacaa > 2:482413/1‑275 (MQ=255) ttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAaacaa < 3:49032/275‑1 (MQ=255) ttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGCGGTGTTGCACTGTCGGAGCGGCGGCAGCTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTTCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCCACCGCGCCAGCTTTGAGAaaca > 2:279824/1‑274 (MQ=255) tGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTAATCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGACA‑GCTGAGCTACGGCGCCTACCATGATGCACTTACGCCTCTCGCCAACCGCGCCAGTTTGgagaccaaa > 2:283508/1‑269 (MQ=255) cAACGGACCCTTAATGGAGAACATTTACAGTGCCGACC‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTATAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATGCAGAACGTTACTGAGTCACGCAAAATGCTGCGCCA‑GCTGCGCTACAGCGCCTACCATGATTCACTGACGCATCACGCCAACCGCCCCGGTTTGCAGAAACAA‑CTGCATa > 3:133603/1‑275 (MQ=255) cAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGCTTTGAGAAACAA‑CTGCGTa > 1:119762/1‑275 (MQ=255) aCCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTTCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCctgc > 1:470049/1‑275 (MQ=255) tgtAATGGAGAACCTTGCCAG‑GCCCAGAGCCTCCCG‑TGCCGGGGTCGGCCAAGAGGTGGTGTTGCATTATGGGGGGGGCGGCAGTTACGACTGGCATTAAATGATTACGCCGTTAAGTACTCTGGAAGGCAGCAATATTGGTTCGGTTCTGGTGAGTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCtgctgc < 3:327860/273‑1 (MQ=255) aTTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCtgctgc < 1:572452/275‑1 (MQ=255) aaggggaaaCATTTAGCGTGCCGACA‑CCTCAAG‑TGCCGCCATCGAACAAGATGTGGGGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCGGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATAACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCaa < 3:468104/267‑1 (MQ=255) aaTGGAGAACATTTACCGAGCCGACC‑CCTCACG‑GTCCGCGATCGAGCAAGATGTGGTGTTGCACGGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATAACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCaa < 3:562749/274‑1 (MQ=255) aaTGGAGAACAGTTACAGTTCCGACA‑CCTCACG‑TTCCGCGTGCGAGCAAGGTGTGGTGTTGCACTGTCGGGGCGGCGGCAGTTACGACGTACATGACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCaa < 3:45686/274‑1 (MQ=255) aggaACATTGACAGTACCGACA‑‑CTTACGCTTCCGGGATCGGGCAAGCTGTGGTGGTGTCAGGCCGGAGCGGGGGAAGTTCGTCCGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGAAGTATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACAATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGt < 3:188864/273‑1 (MQ=255) aCATTTGCAGGGCCGACA‑CCTACCG‑GTTCGGGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGAATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTaaa < 4:482413/274‑1 (MQ=255) aCATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTaaa < 2:302250/274‑1 (MQ=255) aCATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTaaa < 3:470049/274‑1 (MQ=255) ttACAGTGCCGACA‑CCTGACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTTCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTa < 2:174171/275‑1 (MQ=255) gtACAGTGCCGACA‑CCACGCG‑TTGCGCGGTCGAGCAAAATGGGGTGTGGCACTGACGGAGCGGCGGAAGTTACGGAGTGCAGTACGGTATTAGGCCGTTAAGTACTCTGGACGGCAGCAATAGTGGTTCGGTTCGGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTa < 3:116516/274‑1 (MQ=255) gCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTTAGACACCA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCa > 3:73846/1‑275 (MQ=255) ccGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGATCTACAGCGCCTACCATGATGCACTTCCGCATCTCGCCAACCGCACCAGTTTTTAGCAACAA‑ATGCCTA‑TCCTGCTGCAAACGGTAAACAGTACCAATCac > 3:266202/1‑274 (MQ=255) ccGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCTCACCATGCTGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAC‑CTGCGTA‑TCCTCATGCCAACGGTAAACAGTaaccatacg > 3:530050/1‑267 (MQ=255) ccGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAAcc > 4:78063/1‑216 (MQ=255) ccGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGACCACCGTGAGGCACTGACGCATCTCGCCAACCCCGCCAGGTTGGAGCAACAA‑‑TCTGTATTCCTCGTGCAAACGGTAACCacgaaacacaaa > 4:69603/1‑264 (MQ=255) ccGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCCGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACCTGC‑TA‑TCCTGCTGCACACGGAAAACAGTACCCATcga > 4:334958/1‑273 (MQ=255) ccGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGACGGCAGTTACGACGTGCATTACAGTATTACGCCGGTAAGTACTCTGGACGGCAGCAATATTGGTTCGGATCTGGTGATTCAGGACGTTACCGAATCACGCAACATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCat > 1:239007/1‑274 (MQ=255) gACA‑GCTCACG‑TTCCGAGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCg < 2:314349/275‑1 (MQ=255) ca‑cCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGGCATGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc < 3:265214/275‑1 (MQ=255) ca‑cCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCgcgc < 2:78063/216‑1 (MQ=255) cagccgccG‑TCCCGCGTGCGGGCAAGAAGGGGGGTTGAACGGGGGGAGGGGCGGAGGTTACGACGTGCATTACAGTATTACGCCGTTAAGTAGTCTGGGAGGGAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc < 3:537588/268‑1 (MQ=255) aaactaccG‑TTCCGGGATGGAGCAAGAGGTGGGGTTGCACTGTCGGAGCGGCGGCAGTGACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc < 3:92510/268‑1 (MQ=255) a‑cCTCACG‑TTCCGCTGTGGATCAAGAGGCGGGGTTGCACTGGTGGGGCGTCTGTATTTACTCCGTGCATTAAGGCTTGACGCCGGTAAGTACTCTGGAGTGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACATTAACGAATCACGCAAAATGCTGCACCA‑GCTGAGCTACAGCGGCTGCCATGATGCACTGACGCGTCTCGCCAACCGCGCCAGTTTTGAGAGACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATGAGCGACa < 4:73816/275‑1 (MQ=255) cccttaccGCGATCGAGCAAGTGGTGGTGTCGCCCTTTCGGTGCGGCGGAAGTTCCGAAGTTCATTACAGTATTACGCCGTTAAGTACTCTGAACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc < 2:408072/269‑1 (MQ=255) g‑TTCCACGAACGACAAAGGTGTGGTGTTGCACTGTCGGAGTGTCGGAAGTAACGACGTTAATTAAAGTGTTACGCCGTTGAGTACTCTGGACGGCAGAAATATTGGTGCGGTGCTGGTGATTCAGGACGTTACCGAAGCACGCAAAATGCTGCGCCA‑GCTGCGCTAAATCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCGAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCt < 3:369890/274‑1 (MQ=255) ttCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc < 1:73846/271‑1 (MQ=255) gATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAGAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTa < 2:91476/275‑1 (MQ=255) caggtggtgTTGCCCTGTCGGGGTGGAGGCAGGTACGACATGATAGCCAGTATCAACCCGTTTATTACTCCGGTCTCAAACAATATTGATTCGGTCCTGGTAATACAGCACGTTACCGAATCACTCAAAATGCTGCGCCA‑GCGGAGTTACAGCGCCTACCATGATGCACGGATGCACATCGCCAACCGACCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACAACAGCGACATGCCCTGGTGTTTATCGATCTTGa < 3:279027/272‑1 (MQ=255) ttgtgGTGTTGCACTGTCGGGGCGGCGGCAGTTACGACGTGCATTACAGGATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGAt < 3:191855/274‑1 (MQ=255) tgtgGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATc > 4:118155/1‑275 (MQ=255) gtgtTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTGGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCtt > 1:369543/1‑275 (MQ=255) cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCCGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCCACATCCCCTGGTGTTTATCGCTCTTGACCCCGTTaaat > 4:466160/1‑273 (MQ=255) cTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGCATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACGGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATCCGCTGGGGTTTATCGATCTTTCGCGCTTTACAGCgc > 2:273164/1‑274 (MQ=255) tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGCTGa > 1:99102/1‑275 (MQ=255) tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGCTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAACTGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTACACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATTGCTTTAAAGCGGTg > 2:362224/1‑274 (MQ=255) aGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATg < 2:417950/274‑1 (MQ=255) cttGCATTACAGTAGTACGCCGTAAAGGACTCGAGACGGAAGCAATAGTGGTTCGGTTCTGGTGATTCAGAACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCAAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATgcg < 1:295529/272‑1 (MQ=255) tGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGTCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATAGCTTTAAAGCGGTTAATGACAGCGCCAGGAATgcggcg > 4:297043/1‑275 (MQ=255) aTTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCggg < 2:118155/274‑1 (MQ=255) tATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCtt > 1:46619/1‑275 (MQ=255) tATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCGTGATCGCTTTAAAGCGGGGAATGACAGCGCCGGGCATGCGGCGGGTGACGCtt > 1:557328/1‑275 (MQ=255) ttaaggcGTTAGGGACACTGGACGGCAGCAATAATGGTTCGGTTCTGGTTATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTtg < 4:138368/269‑1 (MQ=255) tACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGGCGCTTggc > 1:236490/1‑275 (MQ=255) cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGTTCTTGATCGCTTTAAAGCGGTGAATGCAAGCGGCGGGCATGCGGCGGGTGCCGCTTggctg > 1:16378/1‑275 (MQ=255) cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGGGAATGACAGCGCCGGGCATGCGGCGGGGGACGATTtgctg > 1:548829/1‑275 (MQ=255) gTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGcgcg < 1:266202/275‑1 (MQ=255) ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGTTGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGaga > 3:274778/1‑189 (MQ=255) ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGTATGCGGCGGGTGACGCCTTGCTGCGCGa > 1:266885/1‑275 (MQ=255) ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGa > 2:65584/1‑275 (MQ=255) gTACGCTGGAGTGAAGCAATATTGGTTCGGTTCTGGGGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTTCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTg < 1:428937/275‑1 (MQ=255) actcagaaacgcagcaACATAGAGTCCGTTCTGGTGATTCAGAGCTTTACCCAATAACGCAAAATGCCGCGCCA‑GCAGCGCTACAGGGACGACCATGATGCACTGAAGCATCTCACCAACCGCGACAGTTTTGAGGAACGA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACNTGCCCTGGTGTTTATCGATCTTGGTCGCTTTAAAGCGGTGAATGACAGGGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTAGc < 4:279824/265‑1 (MQ=255) aCTCTGGACGGCAGAAATATGGGTTCGGTTCTGGTAATTCAGGACGTTACCGAATCACACAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc < 3:514107/275‑1 (MQ=255) tctGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACa > 3:585655/1‑183 (MQ=255) tctGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGGGCCGGGCATGCGGCGGGTGACGCTTTCGTGCGCGAACTGGcgg > 3:313693/1‑274 (MQ=255) tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGcgtcg > 1:418039/1‑275 (MQ=255) tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCAAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGACCTGGTGTTTATCGACATTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGcgccg > 3:309311/1‑275 (MQ=255) gACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc < 1:585655/183‑1 (MQ=255) gACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAAAGACCACGCCGGGCATGCGGCGGGTGAGGCTTTGCTGCCGGACCCGGcgtcgtg > 4:65159/1‑274 (MQ=255) ggCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAAt < 1:313693/275‑1 (MQ=255) agcaATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCTCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑CCCTGCTGCAAACGGTAAACAGTCCACATCCGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGTGGAATGACAGCGCCTGGCCTTCGGCGGGTTCCGCTTTGCTGCCCGCACCggtctcgcgacgatc > 4:293819/1‑262 (MQ=255) cattatTTGTTCGGTGCTAGTGATTCATGCCGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTTAGCTACAGCGCCTACCATGATGCACTGACGCATCTCTCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCt < 3:119762/270‑1 (MQ=255) gttggggacTGGTGATTCAAGACGTTTCCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCTCGCCAGTTTTGGGAAACAA‑CTGCGTA‑TCCTGCTGCCAACGCTAAACAGTACACATCAGCGACATGCCCTAGTGTTTATCGATCTTGATCGCTTTAAAGCAGTGAAGGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGGTAATGCTGGGTAtgct < 3:239007/266‑1 (MQ=255) gttcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTAtgctg > 2:196691/1‑275 (MQ=255) gttcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATCCTGAGTAtgctg > 2:492835/1‑275 (MQ=255) cggtttTGGAGAGTCAGGCGTTTACCGAATCACCAAAAATGCTGAGCCA‑GCTGAGCGACAGCGCCGACAATCATGCACTCACGCATCTCGCCAACCGCGCCATTTTTGAGAACCAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCg < 3:16378/274‑1 (MQ=255) aaTCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGAGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGt > 2:112396/1‑275 (MQ=255) aaTGCTGCGCCA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAAt < 2:334958/275‑1 (MQ=255) tgcGCCA‑GCTGAGCTGCAGCGCCTACCAGGTGGCACTGACGCATGTCGCCAACCGCTCCAGTTTTGAGAAACGA‑CTGCGTA‑TGCTGCTTCAAACGGTAGACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGt < 2:8183/275‑1 (MQ=255) tgtgctaaccgAGCTACAGCACCTACCATGATGGCCTGCCTCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CGGCGTA‑TCCTGTTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGGTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGt < 4:196691/265‑1 (MQ=255) ccA‑GCTGGGCTACAGCGCCTACCATGAGTCACTGACGCAACTCGCCAACCGGGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGTATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc < 3:266885/275‑1 (MQ=255) ccA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc < 2:160959/275‑1 (MQ=255) ccA‑GCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc < 2:297043/275‑1 (MQ=255) aGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTAcc < 2:65159/275‑1 (MQ=255) aGCTACAAGGCCTGCCATGATGCACAGACGCAACTCGCCACCCGCGCCAATTTTGGGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAAAAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTAcc < 3:548829/275‑1 (MQ=255) caCAGGGCCTACCATGATGCACTGACGCATCGCGCCGACCGCGCCAGTTTTGAGAAACAA‑CGGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCGGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGAGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGa < 3:99102/274‑1 (MQ=255) gcgcCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTgaga > 3:248422/1‑51 (MQ=255) gcgcCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACATCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGGGGGGATGAATTTGGTCTGCTTCTAACAGACTg > 1:251400/1‑274 (MQ=255) | GAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACA‑CCTCACG‑TTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCA‑GCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAA‑CTGCGTA‑TCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTG > NC_000913/2145042‑2145580 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |