breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsGLY_05_216_S322_L001_R1_001.good.fq409,31857,212,066100.0%139.8 bases149 bases98.2%
errorsGLY_05_216_S322_L001_R2_001.good.fq409,31857,212,066100.0%139.8 bases149 bases97.8%
errorsGLY_05_216_S322_L002_R1_001.good.fq405,25856,075,014100.0%138.4 bases148 bases98.1%
errorsGLY_05_216_S322_L002_R2_001.good.fq405,25856,075,014100.0%138.4 bases148 bases97.8%
total1,629,152226,574,160100.0%139.1 bases149 bases98.0%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,65244.82.1100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000010139
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500024
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.002

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.85249

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.05
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input04:34:47 07 Jan 201904:35:10 07 Jan 201923 seconds
Read alignment to reference genome04:35:10 07 Jan 201904:38:26 07 Jan 20193 minutes 16 seconds
Preprocessing alignments for candidate junction identification04:38:26 07 Jan 201904:38:46 07 Jan 201920 seconds
Preliminary analysis of coverage distribution04:38:46 07 Jan 201904:39:45 07 Jan 201959 seconds
Identifying junction candidates04:39:45 07 Jan 201904:39:52 07 Jan 20197 seconds
Re-alignment to junction candidates04:39:52 07 Jan 201904:40:50 07 Jan 201958 seconds
Resolving best read alignments04:40:50 07 Jan 201904:41:24 07 Jan 201934 seconds
Creating BAM files04:41:24 07 Jan 201904:42:17 07 Jan 201953 seconds
Tabulating error counts04:42:17 07 Jan 201904:42:36 07 Jan 201919 seconds
Re-calibrating base error rates04:42:36 07 Jan 201904:42:37 07 Jan 20191 second
Examining read alignment evidence04:42:37 07 Jan 201904:48:16 07 Jan 20195 minutes 39 seconds
Polymorphism statistics04:48:16 07 Jan 201904:48:17 07 Jan 20191 second
Output04:48:17 07 Jan 201904:48:36 07 Jan 201919 seconds
Total 13 minutes 49 seconds