BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,173,363	Δ2 bp	intergenic (‑1/+1)	gatC ← / ← gatC	pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,173,361	0	C	.	94.7%	380.4 / 7.4	95	pseudogene (2/442 nt)	gatC	pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand): ref base C (2/3); new base . (53/37); total (55/40)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_000913	2,173,362	0	C	.	94.7%	370.7 / 5.9	95	pseudogene (1/442 nt)	gatC	pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand): ref base C (2/3); new base . (53/36); total (55/40)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

ATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCAC‑GGTGACAGGCTCGCCAAATGGACCAAATTTGCGC  >  NC_000913/2173087‑2173634

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atctaaGCCACCATCCCCCCGGCTTTGAACGCACCAACATTAGAACCCATTTGAGTATGCAAACCAATAGTCTGCGTCGCGATCCACAGGGTGATGCCCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATaccac                                                                                                                                                                                                                                                                                    <  4:369341/271‑1 (MQ=255)

  tGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgc                                                                                                                                                                                                                                                                                 >  2:111881/1‑274 (MQ=255)

      tccaccaCACCCCCGGCTTTTAACTCCCCAGCATTAGCCGCCAGTTGGATCTGCAAACCAATAGTTTGCGTCGCGATCCACAGGTTGATGCTCAGAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGGTCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACATCAATTAGAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATacctccgccgta                                                                                                                                                                                                                                                                             <  2:350095/275‑7 (MQ=255)

      gCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACCGCAATTAAAATGG‑TGAGTGGGCTAAAAATCAGGCTTGCCGATaccaccgccgaa                                                                                                                                                                                                                                                                             >  2:254294/1‑270 (MQ=255)

      gCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATCAAGGTCCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACCAAAAACCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTCCCCGGCACCCAAACAGCAATTAACACTG‑ACAGTGGGATAACAATCAGGCTTCCGTATAC‑‑CCCCGCCGt                                                                                                                                                                                                                                                                            >  3:166389/1‑275 (MQ=255)

                       gttaaaCGCCCCAGCATGAGCCGCGATGTGGGTGTGCAAAGCAATAGTGTGCGTCGCGCACCAAAGGGTGATGCTCATAAGCAGGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCGGCAACGc                                                                                                                                                                                                                                                          <  4:111881/272‑1 (MQ=255)

                                   aGCATTAGCCGCCAGTTGGGTGGGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCCCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                                                                                                                                                               <  2:304808/275‑1 (MQ=255)

                                    gCATTCGCCGCCAGTTGGGTGTGCAAACCAATAGTTGGCGTCGAGATCCACAGGTTGATGCTCATAAGTATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                                                                                                                                                               <  3:220538/274‑1 (MQ=255)

                                    gCATTAGCCGCCAGTTGGGTGTGCAGACCAACAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                                                                                                                                                               <  1:166389/274‑1 (MQ=255)

                                         aggcgacAGTTGGTTGGGCAACCCAATAGTTTGCGTCGCGGTCCACAGGGGGAGGCTCAAAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAg                                                                                                                                                                                                                                        <  4:130094/270‑1 (MQ=255)

                                          gccgccAGTTGGGTGTGCAAACCAATCGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAACCGGCAGCACCTTATTACCCGGCACCCAAACAGCAATTAAAATGG‑TGAGTGTGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCCTATGTCCCAGCAGCAACGCCGGATCACGGCAAATCAg                                                                                                                                                                                                                                        >  2:161659/1‑275 (MQ=255)

                                          gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGCCCGCCATCCCCACGAAGAAGCCGAGTGTGGCAAGATCGCCAAACTGCAGCACCTGATTACCCGGCACCCAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCACGCTTCCCGATCCCACCC‑‑CCATATGCACCAGCAGCAACCCCGCATaaacacaacacac                                                                                                                                                                                                                                        >  4:351541/1‑265 (MQ=255)

                                          gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAg                                                                                                                                                                                                                                        >  2:555267/1‑275 (MQ=255)

                                           ccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg                                                                                                                                                                                                                                       <  1:366885/275‑1 (MQ=255)

                                            cgccAGTGTTGTGTGCCAACAAATAGTTTGCGTCGCGATCCACAGGGTAATGCTCATAATGATGACACCCGGGATTAAGGTGCTGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGaa                                                                                                                                                                                                                                     <  4:568825/276‑1 (MQ=255)

                                               cAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGaa                                                                                                                                                                                                                                     >  1:370096/1‑273 (MQ=255)

                                               cAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCACCATCGCCACGAAGAAGCCGATGGTGGCCAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGaa                                                                                                                                                                                                                                     >  1:114618/1‑273 (MQ=255)

                                                  ttGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACCCAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTg                                                                                                                                                                                                                               >  2:468395/1‑276 (MQ=255)

                                                      gtgtGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTg                                                                                                                                                                                                                               >  3:70991/1‑272 (MQ=255)

                                                         tgCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACACCTGAccgccg                                                                                                                                                                                                                        >  2:585704/1‑276 (MQ=255)

                                                         tgCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGACCAAGGCCAATCAGGAACCCCTGAccgccg                                                                                                                                                                                                                        >  2:448197/1‑276 (MQ=255)

                                                                   tAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGcc                                                                                                                                                                                                               >  3:292290/1‑275 (MQ=255)

                                                                        tGCGTCGCGATCCACAGTGTCAGGCCCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGAGGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAGGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTa                                                                                                                                                                                                           <  1:136668/274‑1 (MQ=255)

                                                                        tGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTa                                                                                                                                                                                                           >  3:35143/1‑274 (MQ=255)

                                                                        tGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTAACCCCCGACCTTCGCCTGaaac                                                                                                                                                                                                         >  2:524385/1‑275 (MQ=255)

                                                                        tGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGACCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGt                                                                                                                                                                                                            >  2:454698/1‑273 (MQ=255)

                                                                          cGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGCAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TAAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCACCAAAGCCGTATCAACGCCAACCAGCAACTCCTGACCCCCAACCTCCGCCTGTaac                                                                                                                                                                                                         >  3:125060/1‑273 (MQ=255)

                                                                          agacacggtCCAGAGGGTGATGCGCATAATGATGACACCCGAAATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCAACGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGAAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAAc                                                                                                                                                                                                        <  2:441820/267‑1 (MQ=255)

                                                                                gATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGCTAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTAc                                                                                                                                                                                                  >  1:268862/1‑275 (MQ=255)

                                                                                   acataGGGGGATGCTCATAATAATGATACCCAGGATGAAGGTGATGAACAGAGTTCCACGATGCACGGAGACCGCCATCGCAAAGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCAACTGAGTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                                                                                                                                                              <  4:254294/272‑1 (MQ=255)

                                                                                         ggTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCCCGAAGCAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TTAGAGGTCTAACAATCAGGCTTGCGGATACCA‑‑CCGCCGTATGTCCACGCAGCAACGCCAGATCAAGGCCAAGCAGGAACTCCAGACCGCCGAACTTCGCCGGTAAACGCCTACCAGCCtcat                                                                                                                                                                                         >  2:606661/1‑272 (MQ=255)

                                                                                         ggTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCt                                                                                                                                                                                         <  1:35143/275‑1 (MQ=255)

                                                                                            gATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TCAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCCAGGCCAATCAGCAACTCCTGACCGCCGACCTTCGCCTGGAAACGACCACGATCCCGCTTAg                                                                                                                                                                                      >  2:232518/1‑275 (MQ=255)

                                                                                              tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCAAGCGGCACCGACGGATCAAGGCCAACCAGGAACTCCTGACCGCCGAACTTCCCCCTTAAACGACTCACAGCCTGCTTaccc                                                                                                                                                                                    >  3:387395/1‑272 (MQ=255)

                                                                                                cgcatcatGAGGACACCCGCAATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCCACGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCGATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                                                                  <  3:305287/273‑1 (MQ=255)

                                                                                                cgaaaaaTGATGACACCCGAGATTAAGAGGCGAAACAGATCTCCACGGTCCACGGCGACGGGCATCGCCACGAAGAGCCCGATGGTTGCAATATCGCCAAAGTGCAGCACCTGAATACCCCGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                                                                  <  3:109463/270‑1 (MQ=255)

                                                                                                cTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                                                                  <  1:292290/275‑1 (MQ=255)

                                                                                                 tCAAAATGAGGGAACCCGAGATAAAGGTGGGAGAAAGATTTCCCAGATGAAGGTGGACCGCCATCGCAACGAAGAAGCCGATGGGGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATg                                                                                                                                                                                 <  3:100068/275‑1 (MQ=255)

                                                                                                   aTAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGc                                                                                                                                                                              >  2:434991/1‑276 (MQ=255)

                                                                                                   aTAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑GGAGGGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACCTCGCCTCAAAACGACTACGAGccctcctagagctgg                                                                                                                                                                                >  3:320476/1‑261 (MQ=255)

                                                                                                     catgaagACACCGAGGATTAAAGGGCGGAAAAGATTGCGAGACAGAGCGGCGACCCCAAAGGCCACGAAGAAGCCGATGGATGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGAAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCg                                                                                                                                                                             <  4:127246/274‑1 (MQ=255)

                                                                                                     aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGTCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGt                                                                                                                                                                            >  2:128264/1‑276 (MQ=255)

                                                                                                     aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACt                                                                                                                                                                                                                                   >  2:500626/1‑221 (MQ=255)

                                                                                                     aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGAATACGAACCAGACTAGCGATGGGCGt                                                                                                                                                                            >  4:559426/1‑276 (MQ=255)

                                                                                                     aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CAGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAGACGACTACGAGCCTGCTTAACCATGGGCg                                                                                                                                                                             >  1:337778/1‑275 (MQ=255)

                                                                                                        gatgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCt                                                                                                                                                                                                                                <  4:500626/221‑1 (MQ=255)

                                                                                                         atgaCACCCGAGATTAAGGCGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa                                                                                                                                                                        <  2:559426/276‑1 (MQ=255)

                                                                                                         aggaaccccGAGATTAAGGTTGGGAACAAATTGCCACGCAGGACGGCGACGGCCATCGACACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTa                                                                                                                                                                          <  3:50953/268‑1 (MQ=255)

                                                                                                           gaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGCTTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAACAAGGCAAACCAGGAACTCCGTCCCGCCGAACTTCGCCTGTAAACGACTACAACCCTGCTTGGCCATGGGCGTTAAAc                                                                                                                                                                       >  3:115684/1‑275 (MQ=255)

                                                                                                             cacCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                                                                                                                                                                    >  2:319586/1‑276 (MQ=255)

                                                                                                                   agagTAAGGGGAGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga                                                                                                                                                              <  2:7465/276‑1 (MQ=255)

                                                                                                                   agaTTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATGGCCACGAAGAAGCTGATGGTGGCACGATCGCCAAACGGCAGCACCTGATTACCCGGCACACACACAGCAATTAAAATGG‑TGAGTGGGATAAAAATAAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAGCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCGGTAAACGACAACGAGCCTGCTTAGCCATGGGCGTTAAACCATTCatg                                                                                                                                                               >  2:518290/1‑275 (MQ=255)

                                                                                                                             cgcgAACAGAGTTCCAACACGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAt                                                                                                                                                    <  4:585704/273‑1 (MQ=255)

                                                                                                                              cGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAt                                                                                                                                                    <  1:70991/275‑1 (MQ=255)

                                                                                                                               ggAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAACAAGCGGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACTCGGCATTCAAAAAGCAATTAAAATGG‑TGATTGGGAGAAAAATCAAGCTTTCCGATACCA‑‑CCGCCGAATGTCCCAGCACCAAAGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCCAACTCCGCCTGTAAACGACTAACACCCAGCATCACCTAGGGCTTTAAACCACCCATTACGGGTTTAGTc                                                                                                                                                   >  3:360489/1‑275 (MQ=255)

                                                                                                                                 aaCAGATTTCCACGATGCACGGCAACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAg                                                                                                                                                                                                                                        <  2:236092/188‑1 (MQ=255)

                                                                                                                                      aTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATCATGGGTTTAATAACCAGTg                                                                                                                                            >  2:477000/1‑275 (MQ=255)

                                                                                                                                           cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAA‑CAGGCTTGCCGATACCA‑‑CCGCCGTAGGTCCAAGCAGCAACGACGGATCAAGGCCAAACAGGAACCCCTGACCGCCGACCTTCCCCTGTAAACGACTACAAGCCTGCTTAACGATGGGCGTTAAACCATCCATCAGAGGTTTAATCACCCGTGCCATTa                                                                                                                                      >  4:606024/1‑275 (MQ=255)

                                                                                                                                           cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCCGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCCGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTCCCCGCTGAACTTCCCCTGTAAACGACCACGACCATGCTTAGAGATGGGCTGTAAACCATCCATGAGGCGTTTACGCAcccttcacatt                                                                                                                                       >  4:446025/1‑267 (MQ=255)

                                                                                                                                            aCGATGCACGGCGACCGCCATCGCCACGAAGAAGCAGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAtt                                                                                                                                       <  1:320476/274‑1 (MQ=255)

                                                                                                                                            aCGATGCACGGCCACCGCCATCCCCACGAAGAAGCCGGTGGTTGCAACATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAGGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAtt                                                                                                                                       <  1:115684/274‑1 (MQ=255)

                                                                                                                                                    cGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTACACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCAACCAATATGGGTTTAATCACCGGTGGCATTagcagcctt                                                                                                                              >  3:230789/1‑272 (MQ=255)

                                                                                                                                                          ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGTCGTGACACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTgcc                                                                                                                        >  2:380320/1‑275 (MQ=255)

                                                                                                                                                          ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTCGCATTAGCAGCATCACTgc                                                                                                                         >  3:259312/1‑274 (MQ=255)

                                                                                                                                                                     aCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTTAACCATCCATATTGGGTCTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTAc                                                                                                             >  1:359320/1‑275 (MQ=255)

                                                                                                                                                                       gaagagGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAA‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                                         <  2:606024/276‑1 (MQ=255)

                                                                                                                                                                          gaagCCGACGGGGGCAAAGTCGGCAAACGGCGCAACCTAATTACCCGGCACACAAACAGCAATTAAAATGG‑GGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAg                                                                                                       <  4:454698/276‑1 (MQ=255)

                                                                                                                                                                            agCCGATGGTGGCAAGAGCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGc                                                                                                      <  1:108639/275‑1 (MQ=255)

                                                                                                                                                                             gCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcag                                                                                                                                                                                                                                                                   >  1:185484/1‑117 (MQ=255)

                                                                                                                                                                                 aTGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCagcagc                                                                                                                                                                                                                                                               <  3:185484/117‑1 (MQ=255)

                                                                                                                                                                                  cggtggCAAGAGCGCCCAACGGCAGCACCTGATTACCCAGCACACAAACAGCAATTAAAATGG‑TGTGTGGGATAAACATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGGAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCAGTTTTTACCGCCAGCTGCAAt                                                                                                <  1:68335/274‑1 (MQ=255)

                                                                                                                                                                                     tggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCATCTGCAATcca                                                                                             >  1:163198/1‑275 (MQ=255)

                                                                                                                                                                                          aagatgcaaaaCGTCAGAACCTGATTGCCCGGCACACAAACAGCAATGAAAATGG‑TGAGTGGGATAAAAATCGGGCCTGCCGATACCA‑‑CCGCCGTATGTCCAAGCAGCAACGCCGGAACAAGGCCAATCCGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATNGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTg                                                                                       <  4:319586/268‑1 (MQ=255)

                                                                                                                                                                                            atagCCACACGGCAGAACCTGAATCACCGGCAAACAAACAGCAAGAAAAATGG‑TGAGGTGGATAAAGATCAGGCTTGCCGCTACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTg                                                                                       <  4:555267/271‑1 (MQ=255)

                                                                                                                                                                                             tCGCCCAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTGGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTg                                                                                       <  1:230789/273‑1 (MQ=255)

                                                                                                                                                                                                  aaaCAGCAGCACCTGATTACCCGACACACAAACAGAAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCGTGCCGATA‑‑ACACCGCCATAAGTCCCAGCAGCGACGACGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTAAGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGt                                                                                <  2:71356/275‑1 (MQ=255)

                                                                                                                                                                                                                gATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGCACTCCTTACCGCCGAACTTCGCCTGTAACCGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGATTTTACCGCCAGCCGCAATACACCTTTGACCTCCAAACCCGCGAGGATGc                                                                 >  4:81541/1‑276 (MQ=255)

                                                                                                                                                                                                                gATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGACCTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCTGTGGCATTAGCAGCATCACTGCCGCCGTCTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGACGATg                                                                  >  2:125231/1‑275 (MQ=255)

                                                                                                                                                                                                                  tgccccGGCCCACAAACAGCCATTAAAATGGTTGAG‑CTGATAAACACCAGGCTTGCCGATAC‑‑CCCCGCCCTATTTCCCAGCCGCAACGCCGGATCAAGGCCACTCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCg                                                               <  4:572643/273‑1 (MQ=255)

                                                                                                                                                                                                                     cccGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACTAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTCATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCACCTGCAATACACCTTTGCCAGCGTAACCGGCGCGGATGCCGac                                                             >  3:74195/1‑274 (MQ=255)

                                                                                                                                                                                                                      ccGGAACCCAAACAGCAATTAAAATGG‑TGAGTGGGAAAAAAAGCAGGCCGGCCCATACCA‑‑CCGCCGGATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCg                                                               <  4:128264/272‑1 (MQ=255)

                                                                                                                                                                                                                        ggCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCTCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAAta                                                         >  2:43135/1‑275 (MQ=255)

                                                                                                                                                                                                                        ggCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGCATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATCGGCGTTAAACCATCCATGATTGGTTTAATCCCCCGTTGCATTAGCAGCCTCACTGCCGCCGTTTTTACCGCCAGCTGACATACACCTGTGCCATCCTAACCCGCGAGGATGCCTATAATc                                                         >  2:604993/1‑276 (MQ=255)

                                                                                                                                                                                                                         gCACACAAACAGCAATTAAAATGG‑TGAGTTGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATc                                                         <  2:513196/275‑1 (MQ=255)

                                                                                                                                                                                                                           acacaAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCCCGACGATGCCGATACTCAg                                                       >  2:276278/1‑275 (MQ=255)

                                                                                                                                                                                                                           acacaAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCAGGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGGTTTTACCGCCAGCTCCAATACACCTTTGCACTAGTAACCCGCCAGGATCCAGAttagccg                                                       >  4:189416/1‑269 (MQ=255)

                                                                                                                                                                                                                              caAAAAGCAATTAAAATGG‑GGGGTGGGATAAAACTCAGGCTTGCCGAAAC‑‑CCCCGCCGTATGTCCCAGCAGCACCGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGccc                                                    <  3:268862/275‑1 (MQ=255)

                                                                                                                                                                                                                                aaCAGAAATTAAAAGGG‑TGAGTGGGATAAAAATCAGGCTTGCCCATACCA‑‑CCGACGTACGTCCCAGCAGCAACGGCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCa                                                  <  1:360489/275‑1 (MQ=255)

                                                                                                                                                                                                                                       aTTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCTTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCATGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATCCACCTTTGTCACCGTAACCCGCAAGCATGCCGTCAGTAATCCCCATCACaa                                            >  1:176311/1‑274 (MQ=255)

                                                                                                                                                                                                                                       aTTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACaaaa                                          >  2:260663/1‑276 (MQ=255)

                                                                                                                                                                                                                                             aaggcgtagggggATAAAAATCAGGCTTGCCGATA‑CA‑CCCGCCGCATGTCCCAGAAGCAACGCCGGACCAAGGCCAATCAGGAACTCCTGACCGCCAAACTTCGCCTGTAAACGACTACGAGCCGGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAGCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCa                                      <  3:114618/265‑1 (MQ=255)

                                                                                                                                                                                                                                             aTGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTTGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGGTGCAATACACCTTTGACATCGGAACCCGCCAGGAAGCCGATAATCCGCCCCATCCCAGAACCCa                                      >  1:142552/1‑274 (MQ=255)

                                                                                                                                                                                                                                                g‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATAAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATCGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCCTTGACATCGTAACTCTCGTGGATCCCGATACTCAGCCCCATACCAACACCCCC‑gct                                 >  1:59499/1‑273 (MQ=255)

                                                                                                                                                                                                                                                      tGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCAATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTAACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAACCAC‑GGTGACAg                            >  2:1070/1‑275 (MQ=255)

                                                                                                                                                                                                                                                       gggATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCGGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGCCATCGTAACCCGCGCGGATGCCGATAATCAGCCCCATCCCACAACCACC‑GGTGCCAGGc                          >  4:100819/1‑276 (MQ=255)

                                                                                                                                                                                                                                                         gATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGCATGCCGATAATCAGCCCCATCACAAAACCCAC‑GGTGACAGGCt                         >  1:85405/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                   aGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGCTTTTACCGCAAGCTGCAATACACCTTTGACACCGTAACCCGCGCGGATGCCGAGAAACAACCCACTCGC‑AAACCCACAGGCCACAGGCACCCCaaaacg               >  4:593999/1‑272 (MQ=255)

                                                                                                                                                                                                                                                                                tactcccGCCGAATCGCCCGTCAACAGCTCCGTATAAAGGTCAATGTGTAACTCCTGGCCGCGGAACTTCGCCTGGCACCGACTACGAGCCTGCTTGGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCAC‑GGTGACAGGCTCGCCAAATGGACCAAATTTgcg   <  4:524385/272‑1 (MQ=255)

                                                                                                                                                                                                                                                                                 acccccGCCGTATGTACCAGCAGCAACGCCGGGTCAAGGCCAATCAGGAACTCCGGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCAC‑GGTGACAGGCTCGCCAAATGGACCAAATTTgcgc  <  4:434991/274‑1 (MQ=255)

                                                                                                                                                                                                                                                                                   acccGCCCTAAGGCCCAGCAGCAACACCAGATAAAGACCAAATGGGAACTCCCTACCACCGACCTTCGCCTGTAAACGACTACGGGCCTGCTTAGCTATGGGCGTTAAACCATCCATGGTGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCAC‑GGTGACAGGCTCGCCAAATGGACCAAATTTgcg   <  3:134654/272‑1 (MQ=255)

||

ATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCAC‑GGTGACAGGCTCGCCAAATGGACCAAATTTGCGC  >  NC_000913/2173087‑2173634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`