BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,047	G→T	S59Y (TCC→TAC)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,047	0	G	T	99.0%	299.8 / NA	100	S59Y (TCC→TAC)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (50/49); total (50/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 102 total aligned reads displayed.

AGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGG‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATG  >  NC_000913/4116786‑4117319

aGGATCCACTTCACTTTGGTGCCAGAAAAGTACTGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGcc                                                                                                                                                                                                                                                                    <  2:421048/276‑1 (MQ=255)

aGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCCATTTGATCGGAACTGGTATCGGCTTTCGCCAGCACTTCTACAAGCGTGT‑AGCTTTGGGTGGcc                                                                                                                                                                                                                                                                    >  2:276397/1‑276 (MQ=255)

 gaaacatcttcacttTTGTGCCAAAAAAGTACGGGGCAATCACCAGACCGGTATTGCTGAGGGTATAATCTTCTAAACCGTAACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGcc                                                                                                                                                                                                                                                                    <  4:158338/268‑1 (MQ=255)

     acgacaggattaggTGCCAGAAAAGTACGGGTCAATCCACAGACAGGTATTGCAGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTGTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCGGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGAt                                                                                                                                                                                                                                                               <  4:210320/264‑1 (MQ=255)

       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACACTAGTGGTTTCACGCTGGTTCGTACTACCGATAGCGGCAATGTTAACGGAACTGATATCGGCTTTCGACAGCACTTATACCAGCTTGT‑CGCTTTGGGTGGCCCAGtttt                                                                                                                                                                                                                                                             >  2:577020/1‑276 (MQ=255)

       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATTTCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCTAGCtt                                                                                                                                                                                                                                                              >  1:221872/1‑275 (MQ=255)

       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTTGGTGGCCCAGAtt                                                                                                                                                                                                                                                              >  1:396847/1‑275 (MQ=255)

       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGAtt                                                                                                                                                                                                                                                              >  1:232841/1‑275 (MQ=255)

       acttcacttTGGTGCAAGAAAAGTACGGGTCAATCACCACACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTCAATGCTCGCCGATTTCTGCGGTACCACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGCCAACAGTGTTTTACCGCTGGTTCGTAATCCCCATCACTCCAATTTGTCATGAACTGATCCCTCCTTTCGACCGCTCTTCACCCAGCCGTT‑AGCCTTGGGTCGCCCTGCttt                                                                                                                                                                                                                                                             >  4:138236/1‑276 (MQ=255)

           ccatttaggactaGAAACGTACGGGTAAACTACCAGAGCGGTATTGCGTCTGATATAATTTTCTCAACCGGCACCTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTTCCAGACAATGTCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCa                                                                                                                                                                                                                                                          <  4:372472/263‑1 (MQ=255)

           cacttTGGTGCCAGACAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGCTTTCTGCGGTACGACGGCACTGCAAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGGTAGCTGCAATTGGAGCGGAACTGATATCGTCTTTCTCAAGCACTGCTCCTAGAGTGT‑ATCTTGGTGTgggcggggttttcct                                                                                                                                                                                                                                                         >  4:469353/1‑263 (MQ=255)

             cttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCAtt                                                                                                                                                                                                                                                        <  1:65228/275‑1 (MQ=255)

              tttGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTg                                                                                                                                                                                                                                                       >  2:225231/1‑275 (MQ=255)

              tttCGTGCCAGAAAAGTACTAGTCTATCACCAGAACGGTATTTCTGGGGGTATTATCATCTCAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCAGTACGACGGCACTGCCAGAGAATGGCGTTATATATAGGCGTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAAGTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCttt                                                                                                                                                                                                                                                        <  3:371369/274‑1 (MQ=255)

                tGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGt                                                                                                                                                                                                                                                                        >  1:9262/1‑256 (MQ=255)

                   tGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGCTTTCCAtctttctc                                                                                                                                                                                                                                                  >  2:158338/1‑268 (MQ=255)

                   tGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCTTTGGGTc                                                                                                                                                                                                                                                   >  2:244812/1‑274 (MQ=255)

                   tGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTCCGCCAGCACTTCTACCAGCGTGT‑AGCTTCGGGTGGCCCAGATTTCCATGGGTTc                                                                                                                                                                                                                                                   >  2:436086/1‑274 (MQ=255)

                    gCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGcc                                                                                                                                                                                                                                                                    <  3:9262/256‑1 (MQ=255)

                      cAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGtt                                                                                                                                                                                                                                              <  2:449455/276‑1 (MQ=255)

                       aGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTCCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCACACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCTATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑ACCTTTGGGTGGCCCa                                                                                                                                                                                                                                                                  >  2:210320/1‑255 (MQ=255)

                          aaaggtcgtggcAATCACCAGACCGGTATTGCTGCTGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGTCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAc                                                                                                                                                                                                                                          <  4:107782/265‑1 (MQ=255)

                                cgtgTCAATCACAAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGCCACGTTTTAAATGCTCGCAAAGCTCTGCGGTACGACGGCACTGCCAGACAATCGCGTTATAGATCGGCTTGCAGGTTCCTTTTTCCCAGACAAAAGTGGTTTCACGCTGGTGCGTAATACTGTTATCTGCAATTTGAGCTGAACTGACCCGGGATTTCGCCAGCACTTGTACCACCGTGT‑CGCTTTGGGTGGTCATGTTTACCATTGGGTCGTTTTCTACCaaa                                                                                                                                                                                                                                      >  3:259637/4‑274 (MQ=255)

                                   gTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTgg                                                                                                                                                                                                                                 >  2:85238/1‑276 (MQ=255)

                                        tCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCACATGTTTCCATTGGGTCGGGTTCTACCTAACCTgttgt                                                                                                                                                                                                                              >  4:211142/1‑270 (MQ=255)

                                            cAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCTATAGCTGCAATTTGATCGGAACTGATATCGGCTTTTGCCAGCACTTCTTCCAGCGTGT‑GCCTTTGGGTTGCCCCGTTTTCCCTTTGTTGGTTTTCTTCCCAACAGTGTTTTggtgt                                                                                                                                                                                                                        >  4:460578/1‑273 (MQ=255)

                                             aGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTTATCGGAACTGCTATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCTAGATTTCCTTTGGGTCGTGTTTTACCCAACCTGTTTTTGGGTa                                                                                                                                                                                                                        >  2:179207/1‑275 (MQ=255)

                                                    tATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCTGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGCTTTGc                                                                                                                                                                                                                 >  2:478249/1‑275 (MQ=255)

                                                    tATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCACTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCGTCGTGT‑AGCCTTGTGTGTCCACGATTTCCATTGGGTTGGTTTCTCACCAACCTGGCTTTGGGTAGAGTTGc                                                                                                                                                                                                                 >  3:389472/1‑275 (MQ=255)

                                                              gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTATGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGccc                                                                                                                                                                                                                                                                   >  3:274318/1‑215 (MQ=255)

                                                                  taATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTATGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGAt                                                                                                                                                                                                                                                               <  1:274318/215‑1 (MQ=255)

                                                                    ctattctAAACCGTCACGTTTTAAATGCTCGACGATTTCTGCGGTACGACGGCACTGCCAGTGAATGGTGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                                                                                <  2:469353/273‑1 (MQ=255)

                                                                      cttctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGCAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                                                              >  4:404720/1‑276 (MQ=255)

                                                                        tctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTGGATTTGCTCAAATTCGAGTTGCGACACg                                                                                                                                                                                            >  4:32673/1‑276 (MQ=255)

                                                                        tctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                                                              <  3:232841/274‑1 (MQ=255)

                                                                         ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAATTGATATCGGCTTTCGACAGCACTTCTACCAGCCTGT‑AGCTTTGGCTGTCCCAGATTGCCATTGGGTCGTGTTCTTCCAAACTTGGTTCTGGGGCGATTTCTTTAATTTCCGGCTGCgccccg                                                                                                                                                                                            >  3:187245/1‑270 (MQ=255)

                                                                         ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGc                                                                                                                                                                                           >  4:28900/1‑276 (MQ=255)

                                                                            aaCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCTCTGCGACACGCTa                                                                                                                                                                                         >  1:336470/1‑275 (MQ=255)

                                                                             aCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGGTAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                                                                                                                                                        <  2:348082/275‑1 (MQ=255)

                                                                                 tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCt                                                                                                                                                                                                                                            >  4:281527/1‑219 (MQ=255)

                                                                                 tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCACCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAACTTCGCGCTGCGCCACGCTAATGa                                                                                                                                                                                     >  1:393153/1‑274 (MQ=255)

                                                                                    cGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                                                                                                                                                                                                                         <  2:281527/219‑1 (MQ=255)

                                                                                     gTTGTAAATGCTTGCAGAGTTCTGCGTGACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCGTGCCGGTTGCTTCTTCCCAGACGAGAGTGGGTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGGTATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCGTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTgg                                                                                                                                                                               <  2:126611/276‑1 (MQ=255)

                                                                                      ttttAAAGGCTCGGAGATTTTGGCGGTACGACGGCACTGCCAGACAATGGCGTTATTGATAGGCTTTCCGGTTTCTTTTTACCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTgg                                                                                                                                                                               <  3:155998/275‑1 (MQ=255)

                                                                                         caaaTGCTCGCAGTTTTCTGAGGTACGACGCAGCGGCCAGACAATGGCGTTTTAGGTCGGCTTGCCGGTTTCTTTTTCCCAGACAATATTGGTTTCACGCTGGGTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATc                                                                                                                                                                           <  4:562013/275‑1 (MQ=255)

                                                                                             tgttCGCTGATTTCTGCGGTACGACGGTGCTTCCAGACAATGGCGTTATCGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                       <  4:225231/273‑1 (MQ=255)

                                                                                              ggtagCAGATGTCTGCCGTTCGACGGTACTGACAGACAAGGGTGTTATAGATAGGCGTGCCGGTTGCTTTTTCCCAGACAAGAGTGGTTTCACGCGTGTTCGTAATACCGATAGCTGCAATTTGATCGGAACGGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                       <  4:478249/271‑1 (MQ=255)

                                                                                               cTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                       >  1:274332/1‑274 (MQ=255)

                                                                                               cTCGCAGATTTCTGCGGTACGACGGCACTACCAGACAATGGCGTTATAGATAGGCGTGCCGGTTTCTTTTTCCCAGACAAGAGTGGTTTCACGCTGGTGCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCGTTCGCCATAACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATc                                                                                                                                                                     <  2:232672/276‑1 (MQ=255)

                                                                                                 cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGCCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGAACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTTTTCTACACAACCTGGTTTTGGGTAGCTTTGCTAAATTTCTCGATGCGACACCTCAATGATATTGGAATCGttacact                                                                                                                                                                   >  4:135332/1‑270 (MQ=255)

                                                                                                            tcGGGACTACTGCACTTCCAGACAATGGAGTTATAGTTCTGCTTGCCGGTTTCTTGTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCTCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcg                                                                                                                                                        <  4:276397/275‑1 (MQ=255)

                                                                                                                tACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGTTCGTGTTCTACCCAACCTGGTTTTGGGTCGATTTGCTCCAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGAGCCATTACGACCGCGCgg                                                                                                                                                     >  2:320873/1‑275 (MQ=255)

                                                                                                                         actgcatggcAATGGTGCTATGGTTAGGCTTGCCGTTTTCTTGTTCCCAGGCAATAGTGGGTTCACGCTGGTTCGTAATACAGATGGCTGCAATTTGATCGGAACTGCTATCGGCTTTCGCCAGCACTTCTCACAGCTTGT‑AGCTTTGGGTGGCCCATATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                                                                                           <  4:456079/267‑1 (MQ=255)

                                                                                                                            gccgagcaagtgCGTTATAGATAGGCTTGCCGATTTCTTTTTCCCAGGCAATAGTGGTTTGACGGTGGTTCGTAATACCGATAGCTGCAATTTGATCGGTACTGATATCGGCTTTCGCAAGAACTTCTACCAGTGTGT‑AGCTTTGGGTGGCCCAGATTTCGATTGGGTCGTGTTCTACCCAACCGGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATGTTGGTATCGTGATCCATTACGACCGCGCGGGAGCTGgtggtg                                                                                                                                         <  4:360712/264‑1 (MQ=255)

                                                                                                                              cAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCCGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGAAACGCTAATGATATTGGCATCGTGATCCATTCCGACCGCGCGGGAGCCGGTGGGGc                                                                                                                                        >  1:169921/1‑274 (MQ=255)

                                                                                                                                          ttgtaggtaggCTTGCGCGTTTCTTTTTCCCAGACAATAGTGGGTTCGCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCAGTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAgcgc                                                                                                                           <  3:221872/272‑1 (MQ=255)

                                                                                                                                             tagatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTTAAATTCGCGCTGCGACACGCTACTGCTATTGGCATCTTGATCCATTACGACCGCGCGGTAGCTGGTGGTTCCCTGGTCGTgcgacacg                                                                                                                       >  2:103403/1‑271 (MQ=255)

                                                                                                                                                       tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGAGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGacac                                                                                                                                                                                             >  3:247580/1‑196 (MQ=255)

                                                                                                                                                        gCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATGTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcg                                                                                                                                                        <  1:63651/232‑1 (MQ=255)

                                                                                                                                                           ggTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGAGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                                                                         <  1:247580/196‑1 (MQ=255)

                                                                                                                                                                  ttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCa                                                                                                    <  2:180896/274‑1 (MQ=255)

                                                                                                                                                                  ttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATg                                                                                                  <  2:28900/276‑1 (MQ=255)

                                                                                                                                                                       ccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                                                                             <  2:404720/276‑1 (MQ=255)

                                                                                                                                                                       caagagactggTGGTTTCACTCTTGTTCGTAATACCGATGTGTGCAATTTGGTCTGAACTGTGATCGGCTTTCGCCAGCACTTCAACCAGCGTGT‑AGCTTTGGGTGGCCCAGAGTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                                                                             <  4:179674/266‑1 (MQ=255)

                                                                                                                                                                        cgagcAATATGGGTTGCACGCTGGTTCGTAATACCGATGGCTTCAATTTGATCTGATCTGAGTACGGCTTTCGCCAGGACTGCTACCAGCGTGT‑AGCGTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTCCCCATCCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                                                                             <  3:396847/271‑1 (MQ=255)

                                                                                                                                                                          gACAATTGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAAtt                                                                                           <  2:9530/275‑1 (MQ=255)

                                                                                                                                                                          gACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTCCAGTCATGTTTAATTg                                                                                          >  2:442236/1‑276 (MQ=255)

                                                                                                                                                                            cAATAGTGGTTTCACGCTTGTTCTGAAGACCGATAGCTTGAATTTGTTCGGAACTTCTATGGGCTTTCGCAAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGACCAGATTGCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATNTTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGt                                                                                         <  4:319972/275‑1 (MQ=255)

                                                                                                                                                                                 gtatagggaacagggTTCCGAATACCGAGAGCTGCAATTTGATCGGAACTGTGATCGACTTTCGCCAGCACTTCTACCAGCAGGT‑AGCGTTGGGTAGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                    <  2:224340/262‑1 (MQ=255)

                                                                                                                                                                                  tGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGTGCGCAATGATATATTTTTTTTCATCTATGTTTAATTGTCCCTTa                                                                                   >  1:62588/1‑275 (MQ=255)

                                                                                                                                                                                   ggTTTTACGCTGGGTCGTAATAACGATGGCTGCAATTTTATCGGACCTGGTATCGGCTGTCGCAAGCACTTCTACCAGCGGGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                                                                  <  3:94639/275‑1 (MQ=255)

                                                                                                                                                                                     tttCACGCTGGTTTGTAATACCGATAGCTGCAATTTCACCGGAACTGTTATCGTCTTTCGCCAGCACTTCTACCAGGGTCT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTTCTCAAATGCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCACGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCa                                                                               <  2:138236/276‑1 (MQ=255)

                                                                                                                                                                                       tCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCCGTTATGTTTAAt                                                                                            >  2:319972/1‑261 (MQ=255)

                                                                                                                                                                                          cGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGCGAGCTGGTGGTGCCCTGGTCGAGCGACACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTa                                                                          >  2:538600/1‑276 (MQ=255)

                                                                                                                                                                                                 tCGTAATACCGTTAGCTGCAATTTGATGGGAACTGATATCGGCTTTCGCCAGCAGTTCTACCAGGGTGT‑TGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTTCGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCg                                                                     <  1:389472/274‑1 (MQ=255)

                                                                                                                                                                                                       tACCGTTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                                                                  <  4:199803/255‑1 (MQ=255)

                                                                                                                                                                                                       tACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                                                                  >  2:199803/1‑255 (MQ=255)

                                                                                                                                                                                                          cGAGAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTg                                                           <  2:32673/275‑1 (MQ=255)

                                                                                                                                                                                                            aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGc                                                                                                                                                                                           >  2:89740/1‑145 (MQ=255)

                                                                                                                                                                                                            aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCCTGTTTTATTGTCCTGTAGTCATATTACAGCGAAGCTTTTTGtt                                                         >  1:182872/1‑275 (MQ=255)

                                                                                                                                                                                                            aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTACTATTACAGCGAAGCTTTTTGtt                                                         >  1:87206/1‑275 (MQ=255)

                                                                                                                                                                                                            aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGCCGAGGGCAACGGTATCTTTTTTTTCCATCATGTTTACTTGTTCTGTTGTCATATTACCGCGACGCttttttttt                                                        >  4:222233/1‑275 (MQ=255)

                                                                                                                                                                                                               gCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                                                                                                                                                        <  4:89740/145‑1 (MQ=255)

                                                                                                                                                                                                                cTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGa                                                     <  3:274332/275‑1 (MQ=255)

                                                                                                                                                                                                                 tGCAATTTGTGCGGAACTTATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCTTTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAg                                                   <  2:211142/276‑1 (MQ=255)

                                                                                                                                                                                                                  gCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTCGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGCCGAGCGCAACGATATATTTTTTTTCAGTCCTGTTTTATTGTACCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGaatg                                                  >  2:24910/1‑274 (MQ=255)

                                                                                                                                                                                                                                    atatCGGCTTTCGCCAGCACTTCTACCAGCTTGT‑AGCTTTGGGTGGCCCAGAGTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTttc                                <  2:559521/276‑1 (MQ=255)

                                                                                                                                                                                                                                     tctCGGCTTTCGCCAGCACTTCTACCAGGGTGT‑AGCTTTGGGTGGGCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTttc                                <  3:62588/273‑1 (MQ=255)

                                                                                                                                                                                                                                         ggCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTcc                            >  1:240952/1‑275 (MQ=255)

                                                                                                                                                                                                                                         ggCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATCTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCACCGTGATTCATTACGACCGCGCGGGAGCTGGTGGGGACGCGGCGGAGCGCAACGATATATTTTTCTTAATGCATGGTTAACTGTGACGTCGTTATCTTCACGCGCAGCTTTTTGCTCTTACGAAGTTCTGGTTTCCTTttcttc                             >  3:262748/1‑274 (MQ=255)

                                                                                                                                                                                                                                          gCTTTCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTAGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGAAACGATATATTTTTTTTCAGTCATGTTTAAGTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCcac                          <  2:185320/276‑1 (MQ=255)

                                                                                                                                                                                                                                             ttagCCTGCAATTCTACCAGCGTTT‑AGCTGTTGGTGGCCCATATTTCCATTGGGTCGTTTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCGGCGACACGCTAATGATAGTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCacaac                       <  2:192929/273‑1 (MQ=255)

                                                                                                                                                                                                                                              tagccagatactCTACCTGGGTGT‑AGGTTTGTGTGTGCCAGGTTGCCATTGAGTCGTGTTATACCCAACCTGGTTTTGGTTAGATTTGCTCAAATTCGCGAGGCGAGACGCTAATGATATTGGCAGCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCacaaca                      <  4:179207/265‑1 (MQ=255)

                                                                                                                                                                                                                                              tCGCCAGCACTTCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTCGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTCGTGGTTTCCTTTTCTTCCacaac                       >  1:191061/1‑275 (MQ=255)

                                                                                                                                                                                                                                                   aGCACTTCTACCAG‑GTGGTTGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGa                  <  3:240952/275‑1 (MQ=255)

                                                                                                                                                                                                                                                        gtttaccgtggtgt‑AGCGTCGGGTTGCCCAGATTGCCATTGGGTCGTTTTCTAACAGACCTGTTTGTGTGTATATTTGCTCAAATTCGCGCTGCGACACGCTAATTATCTTGGCATCGTGTTCCATTACGACCGCGTGGGAGCTGTTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGc            <  4:165922/266‑1 (MQ=255)

                                                                                                                                                                                                                                                         tCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGc            <  2:108848/275‑1 (MQ=255)

                                                                                                                                                                                                                                                         tCTACCAGCGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGc            <  2:460578/275‑1 (MQ=255)

                                                                                                                                                                                                                                                                 cGTGT‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTGTGAAGTAGTTGTTGTTTCCTTTTCTTCCACAACACAGATATTGCAAGGCaaa    >  3:97609/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                  gtgt‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTTCGACACGCTAATTATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATCTTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACATCGAAGCTTTTTGTTCTGAATGATTTGTGGTTTCCTTTTCTTCCATAACACTATGATCCCACGGCAAATg  >  4:108089/1‑276 (MQ=255)

AGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGG‑AGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATG  >  NC_000913/4116786‑4117319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`