Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,864,763	A→T	12.0%	intergenic (+29/+19)	yeaD → / ← yeaE	D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,864,763	0	A	T	12.0%	142.8 / 9.9	49	intergenic (+29/+19)	yeaD/yeaE	D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (20/23);  new base T (3/3);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

CCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGC  >  NC_000913/1864615‑1864910                                                                                                                                                        |                                                                                                                                                    ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAACCCTGCA‑CGTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAT‑TTTACGTTAATGTTGTGTGCCGGGT‑GCaa                                                                                                                                                     <  2:944674/149‑1 (MQ=255)  cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGATACGCAAAAAGTGACCAAAGAGAAGCCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAAt                                                                                                                                                    <  1:991216/149‑1 (MQ=255)  cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCaa                                                                                                                                                     <  4:915201/148‑1 (MQ=255)    gatgatGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGTT‑GCaa                                                                                                                                                     >  2:926866/1‑146 (MQ=255)    gatgatGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑CCaa                                                                                                                                                     <  1:926866/146‑1 (MQ=255)      tgatgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCa                                                                                                                                                 >  3:75626/1‑148 (MQ=255)          ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCg                                                                                                                                             >  3:645519/1‑148 (MQ=255)             tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGAT‑GAATTGCACCCGGcac                                                                                                                                         <  1:807999/149‑1 (MQ=255)               cAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGAT‑GCATTGCACCCGGcaca                                                                                                                                        <  4:504157/148‑1 (MQ=255)                 aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGcacaca                                                                                                                                      >  4:546864/1‑148 (MQ=255)                     aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATc                                                                                                                                 >  1:1059023/1‑149 (MQ=255)                      tttgtttgTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAG‑GAAAACTGCACCACCTGCCCAAATCATTCCGCG‑TGCCAAACGGTAATTTTACG‑TAATGTTTGGTTCCCGGTGGCATTCCTTCCGGCCCACAAAATc                                                                                                                                 >  4:252860/1‑148 (MQ=255)                        tgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATcac                                                                                                                               <  4:184948/148‑1 (MQ=255)                                   aaaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAgc                                                                                                                   >  2:106898/1‑149 (MQ=255)                                           tACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGttt                                                                                                            >  3:822431/1‑148 (MQ=255)                                             cGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGttttt                                                                                                          <  3:262058/148‑1 (MQ=255)                                                 tCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                      >  3:850307/1‑148 (MQ=255)                                                  cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCttt                                                                                                     <  4:645519/148‑1 (MQ=255)                                                    aaaaccgaaaaaGTGGCCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                   <  3:252860/141‑1 (MQ=255)                                                         gCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTTGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg                                                                                             >  1:719731/1‑149 (MQ=255)                                                               aGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                                                                           >  2:95288/1‑129 (MQ=255)                                                               aGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                                                                           <  1:95288/129‑1 (MQ=255)                                                                      aaaGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGAT‑GCATTGCACCCGGcaca                                                                                                                                        >  4:264126/1‑93 (MQ=37)                                                                      aaaGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGAT‑GCATTGCACCCGGcaca                                                                                                                                        <  3:264126/93‑1 (MQ=38)                                                                       aaGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATc                                                                                >  3:375451/1‑148 (MQ=255)                                                                          agaAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAg                                                                             <  4:375451/148‑1 (MQ=255)                                                                             aaCCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCAt                                                                          <  3:546864/148‑1 (MQ=255)                                                                                 tGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTa                                                                     >  1:507666/1‑149 (MQ=255)                                                                                            gcgcAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGatat                                                                                        <  2:1070392/120‑1 (MQ=255)                                                                                            gcgcAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGatat                                                                                        >  1:1070392/1‑120 (MQ=255)                                                                                             cgcAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                          <  1:106898/149‑1 (MQ=255)                                                                                                 aTCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaagaa                                                       >  3:1030054/1‑148 (MQ=255)                                                                                                 aTCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaagaa                                                       >  4:92921/1‑148 (MQ=255)                                                                                                     aTTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGtt                                                   <  3:235836/148‑1 (MQ=255)                                                                                                     aTTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGtt                                                   <  4:746849/148‑1 (MQ=255)                                                                                                            ttGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCa                                           >  2:975348/1‑149 (MQ=255)                                                                                                               cGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGGAGAAAGTTCGACCTCAAg                                         <  4:963650/148‑1 (MQ=255)                                                                                                                   aCGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcaca                                     <  3:520562/148‑1 (MQ=255)                                                                                                                     gTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGcc                                  <  2:1059023/149‑1 (MQ=255)                                                                                                                      ttAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGcc                                  <  4:822431/148‑1 (MQ=255)                                                                                                                           tttACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGaaa                                                      >  2:149382/1‑124 (MQ=255)                                                                                                                           tttACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGaaa                                                      <  1:149382/124‑1 (MQ=255)                                                                                                                           tttACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAtt                             >  2:735327/1‑149 (MQ=255)                                                                                                                                gTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                        >  2:892793/1‑149 (MQ=255)                                                                                                                                gTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTGATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGTCGCATTttgtt                        <  1:877222/149‑1 (MQ=255)                                                                                                                                 ttAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGa                                                <  4:816884/124‑1 (MQ=255)                                                                                                                                 ttAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGa                                                >  3:816884/1‑124 (MQ=255)                                                                                                                                 ttAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgttg                       <  1:695077/149‑1 (MQ=255)                                                                                                                                  tAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgttg                       <  4:925582/148‑1 (MQ=255)                                                                                                                                      gttgtGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACAt                  >  2:135448/1‑149 (MQ=255)                                                                                                                                        tgtgtgCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg                <  1:735327/149‑1 (MQ=255)                                                                                                                                               cggat‑GCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc          >  4:6206/5‑148 (MQ=255)                                                                                                                                                   t‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTgg      <  4:113683/148‑1 (MQ=255)                                                                                                                                                       aaTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGc  >  2:466117/1‑149 (MQ=255)                                                                                                                                                       aaTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGc  >  1:1016385/1‑149 (MQ=255)                                                                                                                                                        |                                                                                                                                                    CCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGC  >  NC_000913/1864615‑1864910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑