| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,339,813 | T→C | 7.8% | K85E (AAA→GAA) | mlaF ← | ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,339,813 | 0 | T | C | 7.8% | 364.6 / 15.8 | 115 | K85E (AAA→GAA) | mlaF | ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (56/50); new base C (5/4); total (61/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
AGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTG > NC_000913/3339665‑3339958 | aGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGttt < 1:908380/149‑1 (MQ=255) cGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACTTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCAc < 3:555299/148‑1 (MQ=255) tCCAGCTTCATTATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGtata > 1:798990/1‑149 (MQ=255) ccAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGtata > 4:337538/1‑148 (MQ=255) ccAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGtata > 3:750371/1‑148 (MQ=255) cAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACa > 1:461365/1‑149 (MQ=255) aGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACa > 3:769213/1‑148 (MQ=255) cTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑gc < 2:821614/149‑1 (MQ=255) ttCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑gcg > 2:632228/1‑149 (MQ=255) ttCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑gc > 3:290491/1‑148 (MQ=255) atcatcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGa > 2:794987/1‑149 (MQ=255) tcatcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGa < 3:160070/148‑1 (MQ=255) catcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGaa < 3:527976/148‑1 (MQ=255) catcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGaa < 4:750371/148‑1 (MQ=255) tcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACga < 1:552741/149‑1 (MQ=255) tcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACg < 4:307227/148‑1 (MQ=255) aCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACgaga > 2:594100/1‑149 (MQ=255) cGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGAc > 3:364453/1‑148 (MQ=255) aaaTGCCACAATGGCGCCGGCAGTTTTGTATGTTCGCGCAATCGATAGGCGGCGATGTCAAAAACTTTCATAGCAGTGAACAACGCCCCGGACTGAAAAATCATTGTCATCCGTTTGCGCACTGTATCCCG‑GCGCGAACGCGACACCgt < 2:502132/147‑2 (MQ=255) aTGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGcc < 4:729432/148‑1 (MQ=255) aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGaa > 2:386495/1‑148 (MQ=255) acaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAat < 4:107821/148‑1 (MQ=255) tGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtct < 2:662059/149‑1 (MQ=255) ggCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtct < 3:961426/148‑1 (MQ=255) gcgcGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtctc < 3:836768/148‑1 (MQ=255) ttGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTCTACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa < 2:913327/149‑1 (MQ=255) ttGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa < 1:616882/149‑1 (MQ=255) tGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa < 3:122331/148‑1 (MQ=255) ggTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGa > 1:894143/1‑149 (MQ=255) gTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGAt < 1:843812/149‑1 (MQ=255) ttCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCa > 3:353361/1‑148 (MQ=255) tCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCAc > 4:1009891/1‑148 (MQ=255) gcAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGa < 2:1009781/149‑1 (MQ=255) aGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGAt < 3:75921/148‑1 (MQ=255) ggATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTgg < 1:501761/149‑1 (MQ=255) ggATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTgg < 2:1060175/149‑1 (MQ=255) gATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGt > 1:203546/1‑149 (MQ=255) aTAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGt > 3:1008968/1‑148 (MQ=255) aTAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTg < 2:882768/149‑1 (MQ=255) tAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGc > 1:248820/1‑149 (MQ=255) gCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGa < 4:409746/148‑1 (MQ=255) gCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAt < 2:391106/149‑1 (MQ=255) cGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAt > 4:671415/1‑148 (MQ=255) ttGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGccc < 2:1018985/149‑1 (MQ=255) tCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGc < 3:84738/148‑1 (MQ=255) cAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGcc > 3:328529/1‑148 (MQ=255) aTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAAt > 4:14725/1‑148 (MQ=255) aCGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCa > 3:430182/1‑148 (MQ=255) cGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGa < 2:38559/149‑1 (MQ=255) gTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCg > 2:458753/1‑86 (MQ=255) gTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCg < 1:458753/86‑1 (MQ=255) tatCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACgga < 1:1063353/72‑3 (MQ=21) tatCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACgga > 2:1063353/1‑70 (MQ=21) tCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAg > 2:629448/1‑149 (MQ=255) aGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcg > 1:809051/1‑149 (MQ=255) gTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgt > 1:479632/1‑149 (MQ=255) tGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtc > 1:141302/1‑149 (MQ=255) acaacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtcgt > 4:463290/1‑148 (MQ=255) acaacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGtt < 2:479632/149‑1 (MQ=255) caacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGtt < 3:1009891/148‑1 (MQ=255) caacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGtt < 2:717593/148‑1 (MQ=255) caacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGtt > 1:717593/1‑148 (MQ=255) aacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGtttt > 2:829078/1‑149 (MQ=255) acGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGtttt > 3:744473/1‑148 (MQ=255) cGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTa < 4:1008968/148‑1 (MQ=255) cGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTa < 4:766289/148‑1 (MQ=255) cccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCa > 1:176963/1‑97 (MQ=255) cccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGa < 2:980714/149‑1 (MQ=255) ccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGa > 3:84943/1‑148 (MQ=255) ccGGACCGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGAt > 2:679444/1‑149 (MQ=255) aCTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGccc > 2:231868/1‑149 (MQ=255) aCTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGcc < 4:438374/148‑1 (MQ=255) cTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGccc > 3:698445/1‑148 (MQ=255) gAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCGGCCAATCAGACGGATTAGCGTCGTTTTACCGATGCCCGa > 3:697443/1‑148 (MQ=255) gAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGa < 4:795612/141‑1 (MQ=255) aaaTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGAt > 4:704898/1‑148 (MQ=255) aaTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATgg > 1:537025/1‑149 (MQ=255) aaTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATgg > 1:46830/1‑149 (MQ=255) aTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATgg > 4:718211/1‑148 (MQ=255) tGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCAt > 3:731905/1‑148 (MQ=255) cTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGa > 3:290497/1‑148 (MQ=255) tCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATc > 2:313474/1‑149 (MQ=255) aTCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGc > 1:946157/1‑149 (MQ=255) tCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCt > 4:278393/1‑80 (MQ=255) tCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCt < 3:278393/80‑1 (MQ=255) tCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGcc < 1:679444/149‑1 (MQ=255) tCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGc > 3:63202/1‑148 (MQ=255) ctcGTTCGCGC‑CTGTATACAGTGCGCAAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGcc > 1:383886/3‑149 (MQ=255) tcGTTCGCGC‑CTGTATACAGTGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGa < 2:568613/142‑1 (MQ=255) ccGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCg > 2:103086/1‑149 (MQ=255) cGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCg < 3:463290/148‑1 (MQ=255) cGTTCGCGC‑CTGTATACAGTGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtcg > 4:25289/1‑115 (MQ=255) cGTTCGCGC‑CTGTATACAGTGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtcg < 3:25289/115‑1 (MQ=255) cGTTCGCGC‑CTGTATACAGTGCGCAAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCg > 3:601156/1‑148 (MQ=255) gTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgt < 3:79013/112‑1 (MQ=255) gTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgt > 4:79013/1‑112 (MQ=255) gTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTg > 2:908851/1‑149 (MQ=255) tttGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGa < 3:902739/122‑1 (MQ=255) tttGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGa > 4:902739/1‑122 (MQ=255) tttGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTg > 4:622655/1‑148 (MQ=255) | AGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTG > NC_000913/3339665‑3339958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |