| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,472,434 | G→A | 5.5% | R34* (CGA→TGA) | pyrL ← | pyrBI operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,472,434 | 0 | G | A | 5.5% | 482.2 / 11.8 | 146 | R34* (CGA→TGA) | pyrL | pyrBI operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base G (68/70); new base A (3/5); total (71/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01 | |||||||||||
Only 100 of 146 total aligned reads displayed.
GTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGG > NC_000913/4472287‑4472581 | gTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGa > 2:1039093/1‑149 (MQ=255) tGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGAtt < 1:986987/149‑1 (MQ=255) tGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGAt < 4:778626/148‑1 (MQ=255) gggTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAAGCCCCTCGATTGa > 1:1034152/1‑149 (MQ=255) gggTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGa < 3:677819/148‑1 (MQ=255) gggTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAg < 1:970494/149‑1 (MQ=255) ggTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAgg > 2:628388/1‑149 (MQ=255) gTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAggg > 1:544857/1‑149 (MQ=255) tttGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAgg > 1:171354/1‑147 (MQ=255) tttGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAgg < 2:171354/147‑1 (MQ=255) tttGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATGGAggg > 3:719288/1‑148 (MQ=255) ttGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCAATCGAGGGGc < 2:292988/149‑1 (MQ=255) gCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCt < 3:507254/148‑1 (MQ=255) gCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCAATCGAGGGGCtt < 2:479122/149‑2 (MQ=255) cTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTg < 4:111264/148‑1 (MQ=255) tttCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCt > 2:941504/1‑146 (MQ=255) ttCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCgggg > 3:736442/1‑148 (MQ=255) tCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGa < 4:479250/148‑1 (MQ=255) tCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCAATCGAGGGGCttttt < 4:716164/148‑5 (MQ=255) aGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATg > 1:224004/1‑149 (MQ=255) gTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGGCGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATgg < 2:605124/149‑1 (MQ=255) gTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATgg > 1:1077526/1‑149 (MQ=255) tttCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGGCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATgt > 3:1037217/1‑147 (MQ=255) tttCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAgggg > 3:1031889/1‑138 (MQ=255) tttCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAgggg < 4:1031889/138‑1 (MQ=255) ttCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGaa < 4:31110/144‑1 (MQ=255) ttCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGaa > 3:31110/1‑144 (MQ=255) gccgcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAt < 1:576050/149‑1 (MQ=255) ctgcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAAAGATATGTTTCTGATAGAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAt > 4:658310/3‑148 (MQ=255) ccgcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAt < 3:939844/148‑1 (MQ=255) gCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGgaa < 2:377254/149‑1 (MQ=255) ccAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGgaag > 2:817655/1‑149 (MQ=255) aCCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGaaaa < 3:749906/148‑1 (MQ=255) ccAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGaaaaa < 3:299446/148‑1 (MQ=255) aTTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGCATGGGTGATCAACGGGAAGAAAAACGGCa < 2:288066/149‑1 (MQ=255) ggTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCa > 2:561542/1‑149 (MQ=255) cATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGc < 2:1034152/149‑1 (MQ=255) tCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTc > 2:349291/1‑149 (MQ=255) cgcgACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTc > 4:361494/1‑148 (MQ=255) cTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGACCAGCGTCttttt < 3:76528/148‑1 (MQ=255) cTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑‑AAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTc < 1:48799/149‑1 (MQ=255) tAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCtttttt > 4:638770/1‑148 (MQ=255) tAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTc < 1:243249/149‑1 (MQ=255) gTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGa < 3:577470/148‑1 (MQ=255) tCGTTTATGGAAATGATATGTTTCTGATATAGCGTATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGAc > 4:67309/1‑148 (MQ=255) tttATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGAcgcg > 3:559904/1‑148 (MQ=255) tttATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCgg < 2:132734/149‑1 (MQ=255) tATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTa > 1:893164/1‑149 (MQ=255) aTGGAAATGATATGTTTCTGATATAGCGTATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTa < 3:67309/148‑1 (MQ=255) aTGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTa < 4:313354/148‑1 (MQ=255) ggAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGa > 1:147952/1‑149 (MQ=255) gAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGt < 2:1052407/144‑1 (MQ=255) gAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGt > 1:1052407/1‑144 (MQ=255) aTGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCGCGTGACGGCTGGGC‑AAGAAAAAGCCCCTCGATTGAGGGGCTGGGACTGGGAGATCAACGGGAAGAAAAACGGTAGGGCAGCGTCTTTTTTCAGACGCGGTAAGACaa > 4:652787/1‑148 (MQ=255) tGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACaaaa < 1:1039093/149‑1 (MQ=255) tGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACaaa < 3:1787/148‑1 (MQ=255) tGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACaaa < 3:330033/148‑1 (MQ=255) gATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACaaaa > 4:207658/1‑148 (MQ=255) atatGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAGAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAATACAAAAta > 2:878565/1‑148 (MQ=255) atGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGt > 3:129834/1‑148 (MQ=255) atGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGt < 3:318954/148‑1 (MQ=255) atGTTTCTGATATAGCGGATTAGCCATCGTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTc < 1:789821/149‑1 (MQ=255) tttCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTc > 1:35947/1‑146 (MQ=255) tttCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTc < 2:35947/146‑1 (MQ=255) tttCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGaa < 1:614446/149‑1 (MQ=255) ttCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGAcgcg < 3:587602/130‑1 (MQ=255) ttCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAg < 3:259165/135‑1 (MQ=255) tCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAaca > 1:170699/1‑149 (MQ=255) atatAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACt > 2:535826/1‑149 (MQ=255) atatAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACt > 1:210306/1‑149 (MQ=255) tataGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacaca < 2:390873/146‑1 (MQ=255) taGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTTATCAACGGGAAGAAAAACGTCAGGCCAGCGACTTCTCTCAGACGCGGTAATCCAAAATGTCCAACACACTGa < 4:760087/148‑1 (MQ=255) aGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGaa < 4:716802/148‑1 (MQ=255) aGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAAc > 1:550132/1‑149 (MQ=255) cGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCa < 1:535826/149‑1 (MQ=255) ggATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCa < 3:361494/148‑1 (MQ=255) ccATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACAtcct > 1:965387/1‑149 (MQ=255) tCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCt > 1:294200/1‑95 (MQ=255) cTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGga < 3:794914/71‑1 (MQ=255) cTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTccc < 4:590207/148‑1 (MQ=255) tctcCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCaaa < 4:437677/148‑1 (MQ=255) ctcCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCgg > 1:302053/1‑101 (MQ=255) ctcCTGACGACTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCgg < 2:302053/101‑1 (MQ=255) tcCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTg > 1:214304/1‑149 (MQ=255) tcCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTg < 2:704534/149‑1 (MQ=255) cTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGt < 3:963470/148‑1 (MQ=255) aCGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCg > 3:617593/1‑148 (MQ=255) gggC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATta < 4:559904/148‑1 (MQ=255) ggC‑AAAAAAAAGCCCCTCGATGGAGGGGCCGGGAATGGGTGCTCAACGGGAAGAAAAACGGCCGGCCAGCCTCTTTTTTCAGACGCGGTAAGACAAAATGTCGACCACACTGAACCAGACATCCTCCCGGCAAATTGTCCGGCATtat < 3:652787/148‑1 (MQ=255) c‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATAc > 2:178165/1‑118 (MQ=255) aaaaaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACt > 1:858404/2‑149 (MQ=255) aaaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTAAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCa > 2:891403/1‑149 (MQ=255) ccaGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGt > 2:914481/3‑149 (MQ=255) ccaGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGt > 2:978268/3‑149 (MQ=255) aaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGt < 2:296788/149‑1 (MQ=255) gCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCa > 4:195058/1‑148 (MQ=255) gCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCa > 4:482537/1‑148 (MQ=255) cccTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGACCGGCATTATACTCATCGTCAGaa < 1:913849/149‑1 (MQ=255) cTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAg > 4:685956/1‑148 (MQ=255) tCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgc > 3:269896/1‑148 (MQ=255) | GTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGG > NC_000913/4472287‑4472581 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |