| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,318,095 | C→G | L594F (TTG→TTC) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,318,095 | 0 | C | G | 99.1% | 403.2 / NA | 114 | L594F (TTG→TTC) | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (59/54); total (60/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
ACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGC > NC_000913/2317820‑2318369 | agagTAAACGGGCGTCCCGTGCCCGGTTCTGAATCTCCCGAGATAACGCCGGTCACCCTGGTGAGTAGTTTTTCACAAACCGCCAGACCCAGACCTGGCCACTGGAAATGACGCTGTACGCCCGTTACGACCTGGAAGAAGGGATCAAACCAGCGCACCACTTCTGTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGaa < 4:410870/275‑1 (MQ=255)actgTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGaa < 2:390892/275‑1 (MQ=255) ggTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGCTAGAGAGATATTCGCCACCCGCGCGACCACGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACa > 2:545657/1‑278 (MQ=255) gTAACCTGCCTTTCCATTCCCGGTTCTTAATCAACCTCGATATCGCCGTCCTTCAGGCTGATTGTGTTTGGACAAATCGCCGAACCCAGACCGGTCCCCGGGAAATTACGCTGTACGTCCGTTCCGACCTGGAAGAAGGGATCATACAAGCGCATCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGaa < 3:218838/275‑1 (MQ=255) gTAAACTTGCTGCCCAGTCCCTGTTCTGAATCTACCGAGGTATCGCCGTCCATCATGCTGATCAGTGTTTCACAAATCGACATACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGaa < 1:475462/275‑1 (MQ=255) gTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTATTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCCCCATCCGCGCGAACATGCAAAACTATGCAGCCGCTACCG‑GCTAATTCTATGGCGTTAGTGAACa > 2:89979/1‑277 (MQ=255) cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATCCAACCGGTATCG‑AGGAATTTTATGGCGTTACTGAACAGCTTGGAGAt > 2:303011/1‑278 (MQ=255) gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGCGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCAGGTATCG‑GTGAATTTTATGGCGTTATTGAACCGGTTGgaactg > 2:6625/1‑273 (MQ=255) gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACCTGCAAAACTATACAGCCGTTATCG‑GTGAGTTTTATGGCGGTACTGAACAGGTTGGAGATg > 2:171052/1‑277 (MQ=255) gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGgag < 1:310066/274‑1 (MQ=255) cccATTCCCGGTTCTGAATCTCCCCCGATATCGCCGTCCATCATGCTGATCAGTTTGTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAc < 2:449931/278‑1 (MQ=255) ccATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGCTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAc < 2:195005/277‑1 (MQ=255) attccagtatCTGAATCTACCGAGATCTCGCCTGCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAc < 3:280976/266‑1 (MQ=255) aTCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCa < 1:666886/275‑1 (MQ=255) aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACCCGGATAGAGAGATAATCGCCATCCGCGCGAACATGAAAAACTATACAGCCGGTCTCG‑GGGAATTTTATGGCGTTACTGAACAGGTTGGAGATTGACTGCTGTAACCGGATCgg > 1:3653/1‑275 (MQ=255) aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCg > 1:277694/1‑274 (MQ=255) aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCACGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGACGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGCACATGCAAAACTATACAGCGGGGATAG‑GTGAATTTTATGGGGTTACGGAACAGGTTGGAAGTGACCTGTTGTCAACgaaccg > 3:673831/1‑269 (MQ=255) aCCGAGATATAGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCAAGACCGGTCCCCTTGCAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGTGCCCCACTTCTTTCGCCGGTATCCCCACGCCGGTATAGCTAAAACGGATAGAGAGATAATCGCCATCCCCGCGAACATGCAAAACTATACATCCGGTATCG‑GTGAATCTTATCTCCTTACTGACCATGTTGGAGATGTCCTGCTGTAACTGAATCgg > 3:576547/1‑275 (MQ=255) gaTATCGCCTTCCATCATGCTGATCAGTTTTTCACAAATCTCCAGACCCAGACCGGTCCCCTGCAACTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTGCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGc < 1:508016/275‑1 (MQ=255) cGCCGTCCATCATGCTGATCACTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTGCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAg < 2:147735/278‑1 (MQ=255) acgcggtcTGCTCAGTTTTTACCAAATCGCAGTAACCAGACCGTGCCCCTGGAAATTACGCTGTCAGCCCGTTCCGACCTGGAAGCAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGAGAACACGGATAGAGAGATAATCGCCAGCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa < 3:599585/268‑1 (MQ=255) cgcacgagtCAGTTTTTCCCACCACCCCAGACCCCGACCGGTCCACTTGAAAGTACGCGGTACGCCCGTTCCGACCTGAAAGAGGGGATCAAACAAGCGAACCACTTCTTGTGCCGGTATCCCAACGCCGGTATCGCGAACGCGGATAGAGAGATAATGGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGc < 4:478744/269‑1 (MQ=255) ttcTGAACCGTTTTTGACAAGTCGCCGAACCCAGACCGGTCCCCGGGGAATAACGCGGAACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGGCCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGc < 3:602819/273‑1 (MQ=255) tGATCAGTTTTTCACAAATCGCCAGACCCAGCACGGTCCCCGAGAAATTACGCGGTACGCACCTTGCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCGTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCACCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑ATGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGcaca < 3:346324/275‑1 (MQ=255) aTCAGTTTTTCACAAATCGCCAGACCCCGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATc < 1:179857/275‑1 (MQ=255) aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGACGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGAGAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATACCCTGCTTTAAACGCATTGGATCGCCATTTAAGTCCCCTGGCACAta > 3:298784/1‑274 (MQ=255) aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTAACG‑GTGAATTTTATGGCGTTACTCAACAGGTTGTGGATGACCTGCTGTAAACGCATCGGGTCGCCTTTTAAGGCCACTTGTACATCTgg > 2:199754/1‑278 (MQ=255) aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATGACGCGGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATcctg < 2:6623/278‑3 (MQ=255) aTCAGTGTTTCACACACCGCCAGCCCCCGACCGGGCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGTGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg < 2:149506/278‑1 (MQ=255) actttttCCCAAATCGCCAGACCCAGACCCGTCCCCTGGAAATTAGCCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGAACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg < 3:722044/273‑1 (MQ=255) aatttttCACAAATCGCCCGACCCAGCTCGGTCCCCTAGAAATTACGCTGTCGGCCCGTGCCGACCTTGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCCCGAACACGGATACAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACCGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg < 3:118948/273‑1 (MQ=255) cacaAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCGGGTTCaa > 3:238810/1‑273 (MQ=255) ccacgccccagacccagacCGATCCCCCAGAAATTAGGCTCGAAGCGCTTACCGAGCGGGAAGAAGGGATCAAACAAGCGCACCACTGCTTGCGCCGGTGTCCCCACGCCGGTATCGCGAACACGGATAGAAGGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATaa < 3:344696/267‑1 (MQ=255) caaaggCCAGACCCCGGCCGGTCCCCGAGAACTTGCTCTGTGCGCCGTTTCCGACCTGGAAGAAGGGACAAAACAGGCGCACCACTTCTGTCGCAGGTATCCCCACGCAGGTATCGCGAACAAGGAGAGAGAGATAATCGCAATCACGGCGAACATGCAAATCGATACAGCGGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCGGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg < 3:214018/270‑1 (MQ=255) aTCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAATACTATACAGCCGGTATCG‑GTGCATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTACATAAAGCa > 2:317741/1‑275 (MQ=255) aTCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTAAACAGCCGGTATCG‑GGGAATTTTATGGCGTTACTGAACAGGTTGGAGCTGACCTGCTGTAAACGCATCAGCTCGCCATTTAAGGCCACTAGCACATCCGGTTCAATAAAGCAGTa > 2:318539/1‑278 (MQ=255) tcccagggcccagaacCGTCCCCTGGACATTACGCTGTACTCCCGTCCGCACCGGAAAGAAGGAGTCAACAAAGCGCCCCCCTTCTTTCGCCGGTATCCCCACGCCGGTAGCGCGAACACGGATAGAGAGATAATCGCCATCCGAGCGAACATGCAAAACTATACGGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc < 4:545657/270‑1 (MQ=255) ccagaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGCAAACCCATCGGGTCGCCATTTAAGGCCACTGGCACTACGTGTTCAATAACGCAAGACaaaca > 3:242155/1‑271 (MQ=255) accGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACAt > 1:472117/1‑245 (MQ=255) cGGTACCCTAGAAATTACGCGGTACGCCGGTTCGGACCTGGAAAAAAGGATCAAACAAGCGCACCACTGCTTTCGCCGGTAGCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGc < 1:497181/275‑1 (MQ=255) gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTGCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg < 3:472117/245‑1 (MQ=255) tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATTACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACCTCCGGTTCAATAAAGCATTACAAGCCGAACTGCttt > 3:153260/1‑274 (MQ=255) aaaTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCCCGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAg < 2:457723/278‑1 (MQ=255) aTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac < 1:546817/273‑1 (MQ=255) acgaccgacccaaCCTCGAAGGAGAGATCAAACGAGCGCACCACGGCGTGCGCCGGGATCCCCACGCCCGTATCGAGAATACGGGTAGGGGGATAAGCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAAGTTTATGGAGTTACTGAACAGGTTGGGGATGACCTGCTGTAAANGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGt < 4:373272/265‑1 (MQ=255) ccGTTCCGACCTGGAAGAAGGCATCAAACAAGCGAACCACTTCTGTCGCCAGTAGCCCCACACCGGTATCGCGAACACGGAAAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCg < 2:630814/278‑1 (MQ=255) ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTa > 1:528529/1‑252 (MQ=255) ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGt > 2:24323/1‑277 (MQ=255) ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAACGGTAAATAGTTGGCgg > 1:276955/1‑274 (MQ=255) ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTGGCGTACCACCAGAGGGAAATAGTTGGCgg > 1:296029/1‑274 (MQ=255) gACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac < 3:528529/252‑1 (MQ=255) aaCAAGCGCACCACTTCTTCCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCAcg < 1:460570/275‑1 (MQ=255) ccatTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAAAAGTTGGCGGTGATGTGGTTCATCCCTTCACGCGTTGAAAAc > 1:576329/1‑275 (MQ=255) ttgcGCCCGTAGCCCCACCCCGGTATCGCGAACACGGATAGGGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt < 4:24323/275‑1 (MQ=255) cttCGCCGGTAGCCCAACGGCGGTATCGCGAAAACGGATAGAGAGATACACGCCATCCACGCGAACATGCAAAACTATACAGCCAGTATCG‑TTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAGGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt < 2:203201/277‑1 (MQ=255) ttCACCCGTATCCCCACGCCCGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCACGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGTCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt < 2:638434/277‑1 (MQ=255) aCGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTg > 1:208224/1‑275 (MQ=255) tATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTg > 2:475487/1‑209 (MQ=255) cgAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCtt > 2:443503/1‑278 (MQ=255) cgAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGNTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCtt > 4:374306/1‑278 (MQ=255) cacGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTc > 3:190220/1‑275 (MQ=255) acGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGTAGTACAAGCCTAACTGTTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCCGTGAACACGCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCg > 1:465841/1‑275 (MQ=255) aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGTTGTGGTTTATCATTGCACGCTGTGAAAAATCACGTCGTTCAATTTTCAACTGGTGCGAGTCAATCTTCGAGAAATAGAGAat‑aa > 4:666516/1‑277 (MQ=255) aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGaa > 3:158769/1‑275 (MQ=255) aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAat‑at > 2:264848/1‑278 (MQ=255) aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAat‑at > 2:387398/1‑278 (MQ=255) aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCAGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAat‑at > 2:392416/1‑278 (MQ=255) aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTgg > 4:295536/1‑121 (MQ=255) aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCCCCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCTAGAat‑a > 1:369669/1‑275 (MQ=255) aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGTGGTGAAATCTCACGCGGTTCGATCTTCCACTGTTCCGTTTCAATCTTCGAAAAATCgaacatt > 3:623486/1‑270 (MQ=255) aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGACAACt > 3:165984/1‑222 (MQ=255) aaTCGCCATCCGCGCGAACATGCAAAACTAGACAGCCGGTGTCG‑GTGAATTTTATTGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTAGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAat‑a < 1:673831/275‑1 (MQ=255) aTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGc < 2:295536/121‑1 (MQ=255) gcaccATCCCGGCGAACATTCAATACTATACCGCCGGTAGCG‑TTGAATTTTATGGCGTTAATGAACAGGTTGGAGAGGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCAGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGc < 4:387398/274‑1 (MQ=255) tCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAAt > 4:39908/1‑278 (MQ=255) cgcgAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAt > 2:534727/1‑181 (MQ=255) gcgAACATGAAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAtg < 4:534727/181‑1 (MQ=255) tgaaaaaCTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTcaacaa < 1:190220/272‑1 (MQ=255) tGCAAAACTATACAGCCGGTATCG‑GGGAATTTTATGGCGGTACTGAACAGGTTGGGGATGGCCTGCTGTAAACGCATCGGGTCGCCAGTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTcaacaa < 1:298784/275‑1 (MQ=255) aatcggcTAAACAGGCGGTCTGT‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATAGGGTCGCCATTTAAAGCCACTGGCACATCCGGTTCAATAAAGCAGTACACGCCTAACTGCTTGCGTACCACCGGCGGTAAATGGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTcaacaa < 3:439288/268‑1 (MQ=255) ataCAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTCAACAACAGGCTgg > 1:66383/1‑274 (MQ=255) ataCACGCGGTATCGTGTG‑AGTTTATGGCGTTACTGAGCAGGTTGGAGATGACCTGCTGTAAACGCAGCCGGTCGCAATTTAAAGCCACTGGCACATCCGGTTCAATAAAGGAGTACAAGACTAACTGCTTGGGTACCACCAGCGGTAAATAGTTGGCGGTGATATGGGTGATCACTTCTAGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAAGCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTCAACAACAGGCTgg < 3:606404/274‑1 (MQ=255) taCGGCCGGCATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATGTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTCAACAACAGGCTGGAagag < 2:408540/278‑1 (MQ=255) taCAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGtt < 2:473390/169‑1 (MQ=255) taCAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTGAATCTTCGAGAAATCGAGAAT‑AGCGCGCATAATTTTCAATAACCGGCTGGa > 3:411251/1‑274 (MQ=255) taCAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTCAACAACAGGCTGGa > 1:492912/1‑274 (MQ=255) taCAGCCGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTCAAACACAGGCTGGAaga > 2:494206/1‑277 (MQ=255) ccGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATATTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCAGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTAAACAACAGGCTGGAAGAgttgt > 2:95698/1‑277 (MQ=255) ccGGTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGTTTCAATCTTCGAGACATCGAGAAT‑ATCGCTTGTAATTTTCAACAACAGGATGGAAGCgttgtt > 2:671269/1‑278 (MQ=255) ggTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCAACAGCGGTAAATAGTTGGCGGTGATGTGGATCATCAGTTCACGCGGTGAAAACTCACGCGGTTGGATCTTGAAGTGTTCCGTTTTAATTTTCGAGAAACGGAGAATCAGCG‑TGAGATTTGTCAACAAAGGGGTGGACAAGTGGTTCa > 4:578304/1‑278 (MQ=255) gTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCCACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCCGATAATTTTca > 1:439288/1‑253 (MQ=255) gTATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGG‑TCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTAACAATTTTAAACAACAGGCTGTAAGAGTTGTTCAt > 4:79341/1‑277 (MQ=255) tATCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGa < 4:74405/211‑1 (MQ=255) gtag‑gTCAATTTTATGGCGTTACTGAAAAGGTTGGAAATAACCTTCTGTAAACGCATCGGGTGCCCATTTAAGGCCCCTGGAACATCCGTTTAAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCa < 1:722787/272‑1 (MQ=255) aTCG‑GTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCTCGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTc > 1:552312/1‑274 (MQ=255) tttATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCAc < 3:277694/275‑1 (MQ=255) ttACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGTAT‑ATGGATGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTTCCGTCACCAGCCGaac > 1:520828/1‑273 (MQ=255) ttACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGAc > 2:538521/1‑278 (MQ=255) ttACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTAATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGa < 2:80563/277‑1 (MQ=255) aCTGAACAGGTTGGGGATGACCTGCTGTAAACCAATCGGGTCGCCATTTAAGGCAACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGc < 2:363631/278‑1 (MQ=255) | ACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCG‑GTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAAT‑ATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGC > NC_000913/2317820‑2318369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |