BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,991,844	A→T	D231V (GAT→GTT)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,991,844	0	A	T	98.5%	461.7 / NA	132	D231V (GAT→GTT)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (48/82); minor base G (1/1); total (49/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 132 total aligned reads displayed.

AAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTAC  >  NC_000913/3991575‑3992111

aaacctggCCGCCTCGCTGGGTGGGAAAGTCAGGCTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGGAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCg                                                                                                                                                                                                                                                                        <  3:68858/269‑1 (MQ=255)

 aaCGGGGCCGCCTGGCTGGGGGTGGAAGTCGGCTTCTTCCTGATGGAGGAAAACCGCTTCCGTCCGAATGAAAGCGGCAGCCTGGTGGGCGAAGTTTGTGGGTCCGCCCAGCATATACGGCTGCTTGTCGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGGGCATTACGAC‑GACTATGGGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCgg                                                                                                                                                                                                                                                                       <  3:680417/275‑1 (MQ=255)

      ggCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGGGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTTACGCCAAATGAATGCCTGGTTCTCGGTGGCt                                                                                                                                                                                                                                                                  >  1:600752/1‑275 (MQ=255)

        ccgccTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGGATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GGCTATGTGATGACGCTTTACGCCCAGGGCGGGCTGACGCCAAATGAATGGCTGGTTGTCGGCGGCtt                                                                                                                                                                                                                                                                 >  3:662341/1‑274 (MQ=255)

        ccgccTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCt                                                                                                                                                                                                                                                                  >  1:354580/1‑273 (MQ=255)

           ccTCTCTGGGTGTGGAAGTCAGGTCCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGc                                                                                                                                                                                                                                                             <  3:212735/275‑1 (MQ=255)

             gtgCTGGGGTTGGAGTTTAGCCTCTTGCTTAGTGATGACAGCCGCTTCCCGCATAATGAAGGCGGGAGCCTGGGGGGTGAAGATTGTGGCTACACCCAGCATATACGGCTGCTTGACGAATTTGATCGTACCGCCGGGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCt                                                                                                                                                                                                                                                        <  4:148269/276‑1 (MQ=255)

                cTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCt                                                                                                                                                                                                                                                        <  3:354580/275‑1 (MQ=255)

                cTGGGGGTGGAAGTCAGCTTCAGCCTGATTGATGAAAACCGCTTCCGTCATAAGGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATGACGAC‑GACTATGTGATGACGCTTGACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCt                                                                                                                                                                                                                                                        <  3:291123/275‑1 (MQ=255)

                 tGGGTGTGGAAGTCAGCTTCTTCCTGAGTAATGAAAACCGCGTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCttt                                                                                                                                                                                                                                                      <  2:595596/276‑1 (MQ=255)

                     ggtgGAAGTCAGCTTCTTCCTGCTTGATGAAAACCGCTTCCGTCATAATGAAGGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTctgct                                                                                                                                                                                                                                                 <  2:242223/276‑1 (MQ=255)

                      gtgGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGa                                                                                                                                                                                                                                               >  2:10148/1‑278 (MQ=255)

                             taggcgacttcCTGAGTGATAAAAACCGGTGCCGTCATAATGAAAGCGGCGTCCTGAGGGGCGAAGATTCTGCCTCCACCCAGCATATACTGCGGCTTGACGAAGTTTATCGTACCGCCGTGCGTCTCGGCGGTAGGCGTATTCTGTGGAATATGGGGCCGTGCGACGAAGAAGATCATAACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCGCGCTTTCTGCTGAagag                                                                                                                                                                                                                                           <  3:379496/268‑1 (MQ=255)

                               aGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGATGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGCAAGGGCGGTTTGCGGTGGGTGACGCTGGCTGTCTGCTGAggatgtgtc                                                                                                                                                                                                                                      >  4:242223/1‑272 (MQ=255)

                               aGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAagag                                                                                                                                                                                                                                           >  1:662410/1‑273 (MQ=255)

                               aGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GAATATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCGCGCTTTCTGCTGCAGAGTAc                                                                                                                                                                                                                                        >  2:566702/1‑276 (MQ=255)

                                             tgtcgaagcccGCTTCCGTCATAAGGAAAGCGGCAGCGTGGGGGGCGACGATTGTGGCTCCACCCAGCATATCCTGCTGCTTGACGAATGTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGCATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCtt                                                                                                                                                                                                                         <  4:10148/268‑1 (MQ=255)

                                             ggatgaAACCCGCGTCCGGCATAATGAAAGCGGCGTCCTGGGGGGCTGAGGTTGTTGCTCCACCCAGCATATACTGCTGCTTGACGAATTGTATCGTACCGCCGTGCGTCTCGCCTGTAGGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGGAGGGCGTGATGACGCCAAATGAGTGGCTGGTTCTCGGTAGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGACt                                                                                                                                                                                                                          <  4:119979/275‑1 (MQ=255)

                                                   aaaCCGCTTCCGTCATAATGAAAGCGGCAGCCGGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGc                                                                                                                                                                                                                     <  1:641016/275‑1 (MQ=255)

                                                   aaaCCGCTTCCGTAAGAATGAAAGCTGCAGCGGGGGGGGCGAAAGTTTTGGCTCAACCCAGCTTATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGc                                                                                                                                                                                                                     <  3:120300/275‑1 (MQ=255)

                                                     gccGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGTGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTGTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAg                                                                                                                                                                                                                   <  1:702170/274‑1 (MQ=255)

                                                     aCCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTCTCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAg                                                                                                                                                                                                                   <  1:271953/275‑1 (MQ=255)

                                                       cGCTTCGGTCATAATGTAAGCGGGCGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTa                                                                                                                                                                                                              <  2:412472/278‑1 (MQ=255)

                                                        gCTACCGTAATAAGTAAGGCGGCCGCCTGGGGGGCGAAGCTTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTAc                                                                                                                                                                                                             <  4:101978/278‑1 (MQ=255)

                                                         cTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCTAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTg                                                                                                                                                                                                                                 >  2:135608/1‑257 (MQ=255)

                                                          ttCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTCAGCTGGCTTTCTGCTGAAGAGTACTTTTGTGCCAGCCTTTGGCATctct                                                                                                                                                                                                               >  3:514694/1‑274 (MQ=255)

                                                          ttCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGc                                                                                                                                                                                                                                <  4:135608/257‑1 (MQ=255)

                                                           tCCGTCATAATTAAAGAGGCAGCCTGGGGGGCGCAGTTTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAag                                                                                                                                                                                                          <  4:449237/278‑1 (MQ=255)

                                                               tCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAGTCTCGCTTTCTGCTGAACAGTACTTGCGTGCCCGGCTTTGGAAGCTCAACAaga                                                                                                                                                                                                         >  3:213266/1‑275 (MQ=255)

                                                                     tGAAAGCGGCAGCCTGGGGTGCGAAGATTGTGGCTCCACCCAGCATATACTGGTGCTTGAGGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGAt                                                                                                                                                                                                 <  2:289698/277‑1 (MQ=255)

                                                                      gaagggggCAGCGTGGGGGTCTAGGCTTGGGTCGCCACCCGGTAGATACTGCTGTTTGGCGAATGTGAGCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGGGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTc                                                                                                                                                                                               <  4:481185/272‑1 (MQ=255)

                                                                       aggggggCCGCCTGGGGGGCGAGAATTGGGGCTCCACCTGTCATGTAGTTCGGCTTGACGAATTTTATCGGACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTcc                                                                                                                                                                                              <  4:566702/273‑1 (MQ=255)

                                                                       aaagggcgagacgggggggCGTAGGTGGTGGCGCCACCCAGCATAGACTGCTGGTTGACAAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAACGTATTCTGTGGAATATGGTGCCGTGCGACAAAGAAAAGCATTACGAC‑GACTATTTGGTGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTcc                                                                                                                                                                                              <  4:157833/265‑1 (MQ=255)

                                                                         aGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTcccc                                                                                                                                                                                            >  2:264498/1‑278 (MQ=255)

                                                                                  cgggggggCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAg                                                                                                                                                                                    <  2:616559/275‑1 (MQ=255)

                                                                                    ggggggCGAAGATCGTGGCTACACCCGGCATATACTTGTGCTTGACGAATTTTATCGTACCGCCGTGGGTCTCGCCGGTAAGCTTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGTC‑GACTATGTGATGTCGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCGCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAg                                                                                                                                                                                    <  3:384176/275‑1 (MQ=255)

                                                                                      ggggAGAAGATTGGGGCTCCACCCAGCAGTTACTGCTGCTGGACGAATTTTATCGGACCGCCGGGCGTCGCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGCCGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAg                                                                                                                                                                                    <  3:600752/273‑1 (MQ=255)

                                                                                            aaGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACt                                                                                                                                                                             <  3:662410/274‑1 (MQ=255)

                                                                                                         cACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCCGGGCGTGCTGACGCCAAATGAAGGGCTGGTTCTCGGTGGCTTAGGCTGCCTTTCTGTTGAAGAGTAATTTGGTGCCAGCCGTTGGCCGTCGTAAAAAACTATACCTTCCCCATAAACAGAGGTACTCAAAacacaccgc                                                                                                                                                                >  3:387335/1‑269 (MQ=255)

                                                                                                         cACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTTCACAAGCGTAACGATTCCCCATACAAACCGGTAACGAAAacaaagatg                                                                                                                                                                >  3:79260/1‑268 (MQ=255)

                                                                                                         cACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTCGCCGCTGTACAAGAGTAGCGATTCCAGATTCACAGACGTACTGAAAacaattcacatg                                                                                                                                                             >  4:332064/1‑268 (MQ=255)

                                                                                                           cccAGCATATACGGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTgg                                                                                                                                                            <  2:54061/276‑1 (MQ=255)

                                                                                                             cAGCCTGTACTGGTGCTTGACGAATGTTATCGTACCGGAGTGCGTCTCGCCGCTAAACGTAATCTGGTGAATATGGTGCCGTGCGACGAAGAAGGGCATTACGAC‑GACTATGTGATGACGCGTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGc                                                                                                                                                        <  4:558235/278‑1 (MQ=255)

                                                                                                                cattcaCTGCCGCTTGACGAATTTTATCCTACCGGCGTGCGTCTCGCCGGTAAGCGTATTCTGGGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGc                                                                                                                                                        <  1:391501/270‑1 (MQ=255)

                                                                                                                   ataCTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGTTAAGCGTATTCTGTGGAATATGGGGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTc                                                                                                                                                  <  2:401564/278‑1 (MQ=255)

                                                                                                                      ctgctgCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTc                                                                                                                                                  <  1:97688/275‑1 (MQ=255)

                                                                                                                      ctgctgCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAACGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTCCTGCTGAAGGGTACTTTGGTTCCAGCCTTTGGCAGCTCTTCAAGATTATCGATTCCCCTTATAAAACGGTACTGAAAATACTGCTGCTTGAAGCCTATTc                                                                                                                                                  >  1:150032/1‑275 (MQ=255)

                                                                                                                        gctgctTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTCAGCACGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGTCTTACAAGCGTATCGATTCCCCCTCACAAGCGGTCCTGAAAACACTGCTGCTGTACGGCTACTCTTgg                                                                                                                                              >  4:552991/1‑277 (MQ=255)

                                                                                                                        actgctTGACGAATTTGATCGTGCCGGCGGTCGACTAGCAGGTAAGAGGATTCTGTGGAATATGGTGCCGGGCGACGAAGAAGAACAGTACGAC‑GACGATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCGTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCt                                                                                                                                                <  3:233715/274‑1 (MQ=255)

                                                                                                                         ctgctTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCt                                                                                                                                                >  1:546791/1‑274 (MQ=255)

                                                                                                                          tgctTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTGAAGCTCGCTTCCTGCTGAAGAGTCCTTTGGTGCCAGCCTGTGGCACCCCTAAAAGAGTATGCACTTCCCATACAACGCGGCACTGAACACACCGGTGCTGGGAGCCCGTTCCggg                                                                                                                                              >  3:703750/1‑275 (MQ=255)

                                                                                                                            ctTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGGTCTCGGTGGCTTACGCGCGCTTTATGCTGAAGAGTATTTTGGTACCAGCCTTTGCACGCGCTACAAGAGTATAGCCTCCCCCTACAAAGCGACACCGAAACCACAGCCTCTGGAAGACTATTctggtgaaca                                                                                                                                         >  4:447806/1‑269 (MQ=255)

                                                                                                                             ttGACGAATTTTATCGTACCGCCTTGCGTCGCGCCTGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTTCGAC‑GACTATGTGATGACGCTTTACGCGCAGGTCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGa                                                                                                                                            <  2:447806/274‑1 (MQ=255)

                                                                                                                              ttgcGAATGTTTACGTACCGCCGGGGGTCGAGCCGGGAAGGGTATGCTGTGGAATAGTGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GGCGATGTGATGACGCTTTACGCGCATGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGaa                                                                                                                                           <  3:682413/271‑1 (MQ=255)

                                                                                                                              tGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTTCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAACGGTACTGAAAACAATGCTGCTGGAAGACTATTCCTTGGaa                                                                                                                                           >  3:563758/1‑274 (MQ=255)

                                                                                                                              tGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTc                                                                                                                                                                                               <  3:480525/222‑1 (MQ=255)

                                                                                                                                  gAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGGATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATAccc                                                                                                                                      <  1:147078/275‑1 (MQ=255)

                                                                                                                                                    ttGCGTCTCGCCGGTAAGCGTATTCTGGTGAATAGGGGGCCGTGCGACGAAGAAGAGCAGTAGGAC‑GACTATGTGATGACGCTTTACGGGCAGGGCGTGCTGACGCCAAATGAATGGCTTGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGaa                                                                                                                 <  4:73017/277‑1 (MQ=255)

                                                                                                                                                    ggtggTCTCGCCGGTAGGCGGATTCTGGGGAAGTTGGTGGCGTGCGACGAATAAGCGCATTACGAC‑GACTATGGGATGACGCTTTACGCGCAGGGCTTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGaa                                                                                                                 <  4:113034/274‑1 (MQ=255)

                                                                                                                                                    gTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGaa                                                                                                                 <  2:115737/278‑1 (MQ=255)

                                                                                                                                                          ctcGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCGTTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGatat                                                                                                           >  2:377395/1‑278 (MQ=255)

                                                                                                                                                                ggTAAGCGTAGTCTGTGGAAGATGGTGCCGTGCGAAGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAAc                                                                                                      <  4:419759/277‑1 (MQ=255)

                                                                                                                                                                agTAAGCGGATACTGTGGAATATGGTGCCGTGGGACGAAGAAGAGCATTACGAC‑GACTAGGTGATGACGCTTTACGCGCTGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAAc                                                                                                      <  2:274960/276‑1 (MQ=255)

                                                                                                                                                                            gtgggtgatagGGGGCCGTGGTGCGAAGAAGTGCATTACGAG‑GACTATGTTATGAAGCTTTACGCGCAGGGCGGGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTGAAGCTCGCTGTCTGCTGAAGAGTACTTTGGTGCCAGCCTTGGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCacg                                                                                          <  4:264498/270‑1 (MQ=255)

                                                                                                                                                                                  agttgtgTGCCGTGGGACAGAGAAAAGCATTACGAG‑GACGATTTGATGTCCGATGACGCGTAGGGCGTGCTGACGCCAGATGAATGGCTGGGTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCTGTTGGCAGCTATACAAGAGTATCGATTCCCCATACAAAGCAGTACTGAAAACACTGCGGCTGGAAGCCTATTCCTGGGAATACCAGAACCCACGTCTGCTGGCGAAAGATGTCAAGCAGCGTTTGCACGACgg                                                                                      <  3:33347/269‑1 (MQ=255)

                                                                                                                                                                                         tGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGTAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGCCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGGTTGt                                                                             >  2:140779/1‑277 (MQ=255)

                                                                                                                                                                                         tGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGAt                                                                                >  1:660644/1‑274 (MQ=255)

                                                                                                                                                                                         tGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGACATCAAACACAGTTTGCACGACGGCGAGAt                                                                                >  1:482321/1‑274 (MQ=255)

                                                                                                                                                                                         tGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCCTCTGGCGAAAGAAAAACAACAGAGGTTGCATGACGGCggagttggc                                                                            >  4:79761/1‑270 (MQ=255)

                                                                                                                                                                                         tGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGCACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGCACCCACGTCTGCTGGCGAAAGATAGCAAACAGCGTTTGGACACCGACgagaa                                                                                >  1:427682/1‑273 (MQ=255)

                                                                                                                                                                                         ggCCGTGGGGCGAGAAAGGGCA‑TACGACTGACTATGTGTGGTGGCTTGAGGGGCAGGGCGGGCTTACGCCAAATGAATGGCTCGTTCTCGGTGGCTTAAGCTCGCGTTTTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGAAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTa                                                                            <  4:653304/277‑1 (MQ=255)

                                                                                                                                                                                           ccGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCAAGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATc                                                                          >  2:106366/1‑278 (MQ=255)

                                                                                                                                                                                            cGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCCTACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATGGTATCg                                                                         >  2:1582/1‑278 (MQ=255)

                                                                                                                                                                                              tGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCCTACAAAGCGGTACTGAACACACTGCTGCTGGAAGCCTATTCCTGGGAATACACGAACCCACTTCTGCTGGCGAAAGATACAACACAGGGTTTGCACGACGGGGCGAtgggatagg                                                                        >  4:261719/1‑269 (MQ=255)

                                                                                                                                                                                              tGCGACGAAGAAGAGCAGTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGtt                                                                       <  2:79761/278‑1 (MQ=255)

                                                                                                                                                                                                 ggcgaAGAAGAGCATTACGAG‑TACGATGTGATGACGCTTTACGCGCAGTGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTTGCAGCTTTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGtt                                                                       <  3:546791/273‑1 (MQ=255)

                                                                                                                                                                                                   cgaAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGa                                                                                                                  >  2:391669/1‑230 (MQ=255)

                                                                                                                                                                                                    gaagaaGAGAATGACGAG‑GACGATGGGATGACGCTTTATGCGCAGGGCGTGCTGACGCCAAATGGATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGATGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGtc                                                                  <  2:233181/277‑1 (MQ=255)

                                                                                                                                                                                                        aagggccatACGAC‑GAGTATGTGATGACGCTTTACGGGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGAt                                                             <  2:332064/270‑1 (MQ=255)

                                                                                                                                                                                                           agCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGAt                                                             <  1:199599/275‑1 (MQ=255)

                                                                                                                                                                                                           agCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGAt                                                             <  1:390623/275‑1 (MQ=255)

                                                                                                                                                                                                                 ccgac‑ggCTATGTGATTGCGCTTTGCGCGCAGGGCGTGCTGCCGCCAATTGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTTCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATa                                                        <  3:660644/273‑1 (MQ=255)

                                                                                                                                                                                                                     c‑gacTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTg                                                                                              >  1:171740/1‑232 (MQ=255)

                                                                                                                                                                                                                     c‑gacTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCa                                                   >  1:149762/1‑275 (MQ=255)

                                                                                                                                                                                                                        acTATGTGATGACGCTTTACGCGCAGGGCGTGCTTACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCa                                                   <  1:142841/273‑1 (MQ=255)

                                                                                                                                                                                                                        acTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGa                                                                                                                  >  4:36313/1‑210 (MQ=255)

                                                                                                                                                                                                                         cTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGaa                                                                                                                 <  2:36313/210‑1 (MQ=255)

                                                                                                                                                                                                                          taggtgATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCatgatg                                              <  2:391778/273‑1 (MQ=255)

                                                                                                                                                                                                                          tATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCacg                                                                                          <  3:171740/232‑1 (MQ=255)

                                                                                                                                                                                                                                atgaCGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGa                                         <  1:79260/275‑1 (MQ=255)

                                                                                                                                                                                                                                    cGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCg                                      <  1:563758/274‑1 (MQ=255)

                                                                                                                                                                                                                                       tttACGGGCAGGGGGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACt                               <  2:411719/278‑1 (MQ=255)

                                                                                                                                                                                                                                         tACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATAc                                                                                                                                                                                        >  1:685613/1‑123 (MQ=255)

                                                                                                                                                                                                                                             cgcAGGGCGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAg                                                                                                                                                                                    <  3:685613/123‑1 (MQ=255)

                                                                                                                                                                                                                                                    cGTGCTGACGCCAAATGAATGGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGTGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACgg                  <  2:193608/278‑1 (MQ=255)

                                                                                                                                                                                                                                                              caaaaTGAATGGTGGGTTTTCCGTGGCTTAAGGTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGTCTTACAAGGGTATCGATTCCCCATACCAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTACTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTg            <  1:323618/272‑1 (MQ=255)

                                                                                                                                                                                                                                                              caaaaTGAATGGCTGGTGCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGGGCCAGCCTTTGGCAGCTCTAAAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTg            <  1:213266/272‑1 (MQ=255)

                                                                                                                                                                                                                                                                 aatgaatgGCTGGTTCTCGGTGGCTTAAGCACGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCATATTCCTGGGAATACCCGAACCCACGTCTGATGGCGAAAGAAAACAAAAAGCGTTTGCACGACGGCGAGATTGTCTCGTTTGGTCTTGATCCTTACAGCATGATGCTGGAGCGGGTTACTGAAATACTGACGGCGATTAAAGa        >  3:485233/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                       tgGCTGGTTCTCGGTTGCTTAAGCTCGCTTTTTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTa   <  1:688393/274‑1 (MQ=255)

                                                                                                                                                                                                                                                                       tgGCTGGTTCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTAc  >  1:665161/1‑275 (MQ=255)

AAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGGTAAGCGTATTCTGTGGAATATGGTGCCGTGCGACGAAGAAGAGCATTACGAC‑GACTATGTGATGACGCTTTACGCGCAGGGCGTGCTGACGCCAAATGAATGGCTGGATCTCGGTGGCTTAAGCTCGCTTTCTGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTAC  >  NC_000913/3991575‑3992111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`