BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,937	T→G	K96Q (AAG→CAG)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,937	0	T	G	97.0%	460.7 / NA	135	K96Q (AAG→CAG)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base T (1/3); new base G (69/61); total (70/65)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

ATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTT  >  NC_000913/4116674‑4117211

aTCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACTAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCAAGT‑GTTAAATGCTCGCAGCTTTCTTCGGTTCGTAGGCACTGATCGACAATGGGGTTATAGATAGGCTGGACGGTTCGttttt                                                                                                                                                                                                                                                                      >  2:660554/1‑278 (MQ=255)

  cGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTcc                                                                                                                                                                                                                                                                    <  2:266744/278‑1 (MQ=255)

   tgtaacaTGGACACGGCCCTGAGTCAATTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTcc                                                                                                                                                                                                                                                                    <  2:223679/273‑1 (MQ=255)

    gTCACAAGGACACGGCCCTGAGTCATTTTACAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCa                                                                                                                                                                                                                                                                  <  2:32548/278‑1 (MQ=255)

       acaTGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCa                                                                                                                                                                                                                                                                  <  1:71423/275‑1 (MQ=255)

        aaTGGACACGGCCCTTCATCAATTTCCAGACACGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGAc                                                                                                                                                                                                                                                               <  4:565000/276‑1 (MQ=255)

               acGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGTTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATCGCTGCGGCTATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCCGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGCCTGGCCGGTTTCTTTTTCCCAGCCACTGGTg                                                                                                                                                                                                                                                        >  2:80608/1‑277 (MQ=255)

               acGGCCCTGAGGCATTGTCCAGATAACCCACGTATCAACCGTACCAAACAGCAAGTCACCACGACGTGCACGCTCGCGAGAGCCTTCCAAATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAg                                                                                                                                                                                                                                                          <  1:624082/275‑1 (MQ=255)

                    ccTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGtt                                                                                                                                                                                                                                                     <  1:263070/275‑1 (MQ=255)

                      tGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACg                                                                                                                                                                                                                                                <  2:512651/278‑1 (MQ=255)

                            aTTTTCCCAATAAGCCACGAATCAACCGGACCAAACCGCAATTCACCACGGATTGCATGGTCGCGGGAGCCTTCCACATGGTGGAGGATCAACTTCCCTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTg                                                                                                                                                                                                                                             <  3:493214/275‑1 (MQ=255)

                             ttttCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGACAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGCCACTGCCAGACCATGGCGTTATAGATAGGCTGGCCGGTTTCTTGTTCCCAACCCATATGGGTTTAACGCTgg                                                                                                                                                                                                                                            >  3:725375/1‑275 (MQ=255)

                             ttttCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGCCGGTACTGCCAGACCCTGGCGTTATAGATCGGCTTGCTGCCTTCTTTTTCCCTGAAAATACTGGTTTCACgtcggtct                                                                                                                                                                                                                                         >  4:511614/1‑271 (MQ=255)

                             ttttCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCtt                                                                                                                                                                                                                                                                         >  1:723591/1‑246 (MQ=255)

                             ttttCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTgg                                                                                                                                                                                                                                            >  3:558012/1‑275 (MQ=255)

                                         gCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCg                                                                                                                                                                                                                                >  3:188607/1‑275 (MQ=255)

                                                 tCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAAt                                                                                                                                                                                                                     <  2:296515/278‑1 (MQ=255)

                                                   aaCCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCATGCTGGTTCGTAATACCGATAGCTGCaa                                                                                                                                                                                                                      >  1:554055/1‑275 (MQ=255)

                                                        tACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCg                                                                                                                                                                                                              >  2:615982/1‑278 (MQ=255)

                                                                          cacGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGCCAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATGGTGGTTTCACGCGGTTTCGTAATCCCGATAGCGGCAATTTGATCGGAATTGaatgcgg                                                                                                                                                                                                 >  3:176907/1‑267 (MQ=255)

                                                                                 ttcACTCTCGCGTGAGCCTTCAACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATCCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAAGAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCa                                                                                                                                                                                        <  1:741363/273‑1 (MQ=255)

                                                                                  gCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATCTAATCTTCTACACCGTCACGT‑TTTAACTGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGCCAATGGCGTTATAGATAGGGCGGTCGGTTTCTTTTTCCCAGCCAATGGGGGGTTCACGCTGGTTCGGAATACCGTTTGTTGCAATCTGCCCGGAACTGATATCGGCTTTcccccg                                                                                                                                                                                       >  3:643405/1‑273 (MQ=255)

                                                                                                  ctcccACATGGTCGAGGAGCCACTTCACTTTGGTGCCAAAAAAGGACGGGTCAATCACCAGAACGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑GTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTg                                                                                                                                                                       <  1:283693/272‑1 (MQ=255)

                                                                                                  cTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTg                                                                                                                                                                       <  1:176907/275‑1 (MQ=255)

                                                                                                  cTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAACTACGGGTCAATCACCAGACCGGTATTGCTGCGAATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTg                                                                                                                                                                       <  1:270436/275‑1 (MQ=255)

                                                                                                                aGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGAc                                                                                                                                                                                                                                                               >  3:4797/1‑173 (MQ=255)

                                                                                                                aGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGc                                                                                                                                                         >  1:584502/1‑275 (MQ=255)

                                                                                                                     ccACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCTTGGAGCTTTGGGGTGCCCAGa                                                                                                                                                    >  1:109895/1‑275 (MQ=255)

                                                                                                                       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTCTAGATAGGCTGGCCGGTTTCTTTTTCCCATACAATAGTGGTTTCACTCTGGTTCTTAATACCGTTAGTTGTAATTTGATCGGAACTTCTATCGGCTTTCGCCTGACCTTCTTCCAGCGTGACGCTTT‑GGTGGccgcgggattcc                                                                                                                                              >  4:150444/1‑268 (MQ=255)

                                                                                                                       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTTACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTCCCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                                                                                                               >  2:670724/1‑278 (MQ=255)

                                                                                                                       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGGTTTCGCCAGCACTTCTACCAGAGTGGGGTGTTGGGTGGCCCGGaat                                                                                                                                                  >  3:572942/1‑273 (MQ=255)

                                                                                                                       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTGGCCAGCACTTCTACAAGCTTGGAGCTTTGGGGGGCCCAGAttcca                                                                                                                                               >  2:37873/1‑275 (MQ=255)

                                                                                                                       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                                                                                                               >  2:5228/1‑278 (MQ=255)

                                                                                                                          atcagtgggTGCCAGAAAAGAACGGGTCAAGCGCAAGACCGGTATTGCTGCGGATATAGTCTGCTGAACAGTCACGT‑TTTAAATGCTCGCAGTTTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGGTAGGCTTGCCGGTTTCTTTTGCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                                                                                                            <  4:428762/271‑1 (MQ=255)

                                                                                                                           cacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGATGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATGGGAACTGATATCGGCTTTCTACAACACTTATACCAGCTTGGAGCGTTGTGTGGTCAAGAttttacttg                                                                                                                                           >  4:575615/1‑272 (MQ=255)

                                                                                                                                 ggTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg                                                                                                                                         <  3:665225/274‑1 (MQ=255)

                                                                                                                                  gTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTTATCGGTACTGATATCGGCTTTCGCCAGCACTTCTACCAGTGTGGAGCTTTGGGTGGCCCATATTTCCATTGGGTCgt                                                                                                                                     >  2:248381/1‑277 (MQ=255)

                                                                                                                                  gTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGt                                                                                                                                        <  1:107954/274‑1 (MQ=255)

                                                                                                                                  gTGCCAGAAAAGGACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAGTCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGGTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCGGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                       <  1:605271/275‑1 (MQ=255)

                                                                                                                                   tGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGGCGACTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTATTACAGATCGCTGCAATTTGATCAGCACTTATATCGGCTTTCGCCAGCACTTCGACCAACGTGGATATTTGGGTGGACCATATTTCCATTTGCTCgttt                                                                                                                                   >  4:21402/1‑276 (MQ=255)

                                                                                                                                   tGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGGTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGt                                                                                                                                        >  1:487293/1‑273 (MQ=255)

                                                                                                                                   tGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCCCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                       >  3:490590/1‑274 (MQ=255)

                                                                                                                                       aGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGG‑TTTAAATGCTCGCAGATTTCTTCGGTACGATGGCACTGCCAGACACTGGCGTTATAGAAAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGTTGCAAGTTGATCGGAACTGCTATCGGCTTTCGCCAGCCCTTCTACCAGGGTGGAGCTTTGGTTGGCCCAGATGTCTATTTGGTCggttt                                                                                                                                  >  3:504796/1‑271 (MQ=255)

                                                                                                                                          aaaGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa                                                                                                                               <  1:188607/275‑1 (MQ=255)

                                                                                                                                                  ggTAAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTTAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTTCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGAAAATAGTGGGTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATAGCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAcc                                                                                                                        <  1:10877/274‑1 (MQ=255)

                                                                                                                                                   gTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                       <  3:362804/258‑1 (MQ=255)

                                                                                                                                                   gTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtt                                                                                                                   >  2:340182/1‑278 (MQ=255)

                                                                                                                                                    tcacgcaccacACCTGTTGTGGTGCGGATCTACTCCTCTAAACCGGCACGT‑TTTAACTGCTCGCAGATTTCTGCGGTCCGCCGGCACTGCCAGACAATGGCGTTATATATAGTCTTGGCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGt                                                                                                                    <  4:573873/271‑1 (MQ=255)

                                                                                                                                                       ctCACCAGACCTGTATTGCTTCGGATATAATCTTATACACCGTCACGT‑TGTAAATGCGCGCAGATTTCTGTGGTACGACGACACTGCCATACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                                                                               <  2:131995/277‑1 (MQ=255)

                                                                                                                                                        tCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtttt                                                                                                                 >  3:467894/1‑275 (MQ=255)

                                                                                                                                                             aGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCAc                                                                                                                                                                                                                                                 >  3:681740/1‑142 (MQ=255)

                                                                                                                                                             aGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTggg                                                                                                              <  1:410902/273‑1 (MQ=255)

                                                                                                                                                             aGAACGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGCCAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGCTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTAGCAAGCCATTCTACCAGCGTGGAGCTTTTGCTGGCCCAGGTTTCCATTGGGTCATGTTTTACCCAACCTGGTTTTGGGTa                                                                                                            >  1:679915/1‑275 (MQ=255)

                                                                                                                                                                         ctgcGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATc                                                                                                                                                                                                   >  1:448257/1‑176 (MQ=255)

                                                                                                                                                                           gctgctataTTCTCCTAAACC‑TTACGTGTTTAATTGCTCGGTGGTTTCTGCGGTACGAGGGCGCTGCCAGACAATGGCGTTATTGATAGGCTGGCCTGTTTCTTTTTCCCAGACAATAGTGGTTTCCCGCTGGATCGTAATACCGATAGCTGCAATTAGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcg                                                                                            <  4:312245/272‑1 (MQ=255)

                                                                                                                                                                           gcGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCAc                                                                                                                                                                                                                                                 >  1:627228/1‑128 (MQ=255)

                                                                                                                                                                            cGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgcg                                                                                          <  4:615982/278‑1 (MQ=255)

                                                                                                                                                                             ggATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCt                                                                                                                                                                                               <  3:448257/176‑1 (MQ=255)

                                                                                                                                                                              gATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCCCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAGTTCCATGTGGTCGTGTTATACCCCACCTGGTTTTGGGTAGCTTTGCTCAAATTcgc                                                                                           >  3:354353/1‑275 (MQ=255)

                                                                                                                                                                              gATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCTTTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCt                                                                                        >  4:579437/1‑278 (MQ=255)

                                                                                                                                                                               atatAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTg                                                                                                                                                                                                                                             <  3:627228/128‑1 (MQ=255)

                                                                                                                                                                                  tcaccttctAAACCATGTCGT‑TTGAAATGCTGGCAGGTTGCTGCCGTACGCAGGCACTGGCAGACAATGGCGGTATAGATTGGCTGGCCGGTTTCTTTTTCCCATACAATAGTGGTTTCACGCTAGTTCGTAATACAGATAGCTGCAATTTGATCGGAACTGATATCGGCTTGCGCCAGCACTTCTACCAGCGTGGAGCTTTGTGTGGCCCAGATTTCCAGTGGTTCGTGTTCTACCCAACCGGGTTTTGGGTAGATTTGCTCAAATTCGCGCTTCGa                                                                                    <  4:490076/274‑1 (MQ=255)

                                                                                                                                                                                  taAGCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                    <  2:154138/278‑1 (MQ=255)

                                                                                                                                                                                   aaTCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCgcgc                                                                                         >  1:526800/1‑272 (MQ=255)

                                                                                                                                                                                     tcttctAAACCGTCACGT‑TTTAAAGGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGAGAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                  <  2:485241/277‑1 (MQ=255)

                                                                                                                                                                                         ctAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCCTTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTAGCGCTGCGACACGCTa                                                                             >  2:454102/1‑278 (MQ=255)

                                                                                                                                                                                         ctAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCACCCTGGTTTTGGGTAGATTTGCTCAAATTTGCGCTGCGaca                                                                                  >  1:162305/1‑273 (MQ=255)

                                                                                                                                                                                         ctAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                  >  3:83621/1‑273 (MQ=255)

                                                                                                                                                                                         ctAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGG‑TCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGc                                                                               >  1:502491/1‑275 (MQ=255)

                                                                                                                                                                                          tAAACCGTCACGT‑TTTAAATGCTGGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                                            <  2:511614/278‑1 (MQ=255)

                                                                                                                                                                                          tAAACCGTAACGT‑TTTAAATGCTCGAAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCTGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                             <  2:481395/277‑1 (MQ=255)

                                                                                                                                                                                             aCCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCt                                                                              <  1:83621/273‑1 (MQ=255)

                                                                                                                                                                                                 tCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATCGCTGCAATTTGATCGGAACTGATATCGGCTTTAGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGGCCAGATTCCCATTGGTGCGTGTTCTACCCAACCTGGTGTTGGGTGAATTTGGCTAATTTCGCGGTGCCACCCCCTAATgtatgt                                                                     >  4:531554/1‑273 (MQ=255)

                                                                                                                                                                                                 tCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGa                                                                         >  1:698122/1‑274 (MQ=255)

                                                                                                                                                                                                     gT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtg                                                                                                                                    <  1:488675/211‑1 (MQ=255)

                                                                                                                                                                                                         ttAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTATTGATATTGGCATCg                                                              >  2:1511/1‑278 (MQ=255)

                                                                                                                                                                                                         ttAAAGGCTCGCAGATTGCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATGGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCa                                                                 <  1:98523/275‑1 (MQ=255)

                                                                                                                                                                                                          tAAAAGCGCGCAGATTTGTGCGGTACGACGG‑‑CTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTGCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                           <  2:62683/278‑1 (MQ=255)

                                                                                                                                                                                                            aaTGCTCGAAGATTTCTGCGGTACGACGGAACTGCCAGACAATGGCGTTATAGGTAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATGTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                           <  4:603201/278‑1 (MQ=255)

                                                                                                                                                                                                             aTGCTCGCAGATTTCTGCGGTAGGACGGCACTGCCAGACAATGGCGTTATAGTTAGGCTGGCCTGTTTCTTGTTCCCAGACAATATGGGTTTCACGCTGGTTCGTAATACCGATAGGTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                           <  2:21402/277‑1 (MQ=255)

                                                                                                                                                                                                             aTGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGAAAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGt                                                             <  1:395774/275‑1 (MQ=255)

                                                                                                                                                                                                               gtgcGCATATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATGGATAGGCTAGCCGGTTTCTTTGTCCCAGACAATAGTGGTTTATCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTGGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                           <  3:679915/272‑1 (MQ=255)

                                                                                                                                                                                                               gCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                           <  1:558012/275‑1 (MQ=255)

                                                                                                                                                                                                                  cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATcc                                                        >  1:619992/1‑275 (MQ=255)

                                                                                                                                                                                                                               gatACGACGGCACTGCCAGACAAAGGCGTTGTAGATAGGTTTGCCGGTTTCCTTTTCCCAGCCAATATTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcg                                            <  3:554055/272‑1 (MQ=255)

                                                                                                                                                                                                                                gTACGACGGCCCTGCCAGAAGATGGGGTTATAGGTTGGCTGGCCGGTTTCTTTTTCCCAGAGGATAGTGGTTCCACGCTGGTTCGTAATACCGAAAGCTACAATTTGATCGGAAGTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCggg                                        <  4:80608/277‑1 (MQ=255)

                                                                                                                                                                                                                                   cgacgGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                     <  2:579437/277‑1 (MQ=255)

                                                                                                                                                                                                                                      cgGCACTGCCAGACAACGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTCCGACACGCTAATGATATTGGCTGCGTCATCAATTACTACCGCGTGGGTGCtgg                                  >  2:424250/1‑277 (MQ=255)

                                                                                                                                                                                                                                         aacgtacAGACAAGGGCGTTTTAGATATGCTGGCCGGTTTCTGTTTCCCAGACACTAGTGGTTTCACGCTGGTTCGTAATACCTATAGCTGCAATGTGATCGGAACGGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCGCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                               <  4:646118/271‑1 (MQ=255)

                                                                                                                                                                                                                                          aCTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTGTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATTGCTGCAATTTGATCGGAACTGTTATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATAGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                <  1:677278/275‑1 (MQ=255)

                                                                                                                                                                                                                                           cTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                               >  1:666183/1‑275 (MQ=255)

                                                                                                                                                                                                                                           cTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGGTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCAATAGCTGCAATTTGATAGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACGCACCCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTCATGATATTGGCATCGTGATCCATTACGACCGTGAggggggtgtggg                               >  3:714363/1‑266 (MQ=255)

                                                                                                                                                                                                                                                      tGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCCGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTTTTCTCCGCACCCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGAGCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCg                    >  3:47160/1‑275 (MQ=255)

                                                                                                                                                                                                                                                      tGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCCTTGGGCCGTGTTCTTCCCAACCCGGCTTTGGGTAGATTTACTTAAGTTCGCTTTTTATCACGCTAATGATTTTGGCTTCGTGAGCCATTATGACCGCGCGGTCGCGGGTTGTGcgtttgcctggg                 >  4:126451/1‑267 (MQ=255)

                                                                                                                                                                                                                                                      tGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAGTGGGGCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGTGCTGCGACACGCTAATGATATTGGCATCGTGATCAATTACGACCGCGCGGGAGGTGGTGGTGCCCTGGtggtgg                 >  4:14942/1‑273 (MQ=255)

                                                                                                                                                                                                                                                              tagatagGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATANCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTGTGGGTAGATTTGCTCAAATTCGAGCTGCGAACCGCCAATGATATGGGCATCGTGACCCATTAAGACCGCGCGGGAGAGTGTGGGGCACTGGTCGAggggaaggag         >  4:347862/1‑269 (MQ=255)

                                                                                                                                                                                                                                                                    ggCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                  <  2:331269/199‑1 (MQ=255)

                                                                                                                                                                                                                                                                    ggCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTTGCATCGTGATCCATTACGACCGCCCGGGAGCTGGTGTTGCCCTGGTCGTGCGCAACGatatctg    >  4:411617/1‑274 (MQ=255)

                                                                                                                                                                                                                                                                     gCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAg                  <  2:411617/262‑1 (MQ=255)

                                                                                                                                                                                                                                                                     gCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTCCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTAAGCCCGCGCGGGAGCTGGTGGGTGCTTGGTCGAGGGAAACGATATAgttt  >  4:220589/1‑278 (MQ=255)

ATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGT‑TTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTT  >  NC_000913/4116674‑4117211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`