| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 291 | A→T | 9.6% | intergenic (+36/‑46) | thrL → / → thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 291 | 0 | A | T | 9.6% | 370.9 / 21.3 | 112 | intergenic (+36/‑46) | thrL/thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base A (50/51); new base T (4/7); total (54/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
TAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCC > NC_000913/143‑438 | taGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGa < 4:1311610/149‑1 (MQ=255) ggCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACa < 4:1476971/149‑1 (MQ=255) ggCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACa < 2:1120392/149‑1 (MQ=255) tAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGc < 3:341529/149‑1 (MQ=255) gcgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCgg > 1:895806/1‑149 (MQ=255) gcgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCgg < 3:778646/149‑1 (MQ=255) gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGc < 4:614513/149‑1 (MQ=255) gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGc < 2:115675/149‑1 (MQ=255) gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCACCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGc < 1:1532683/149‑1 (MQ=255) acagacagATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑tt < 1:519823/149‑1 (MQ=255) gacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑ttttt < 2:816669/149‑1 (MQ=255) gacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑ttttt > 2:80604/1‑149 (MQ=255) acagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTATCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑tttttt > 1:834492/1‑149 (MQ=255) acagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGC‑tttttt < 1:254628/149‑1 (MQ=255) gaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAAGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑ttttttttt < 1:1540399/149‑1 (MQ=255) aTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTc < 4:126002/149‑1 (MQ=255) aTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTc < 2:694816/149‑1 (MQ=255) aTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTc < 3:1104637/149‑1 (MQ=255) tAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCg > 3:651471/1‑149 (MQ=255) aaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGAc < 3:273708/149‑1 (MQ=255) ttACAGAGTACACAACATCCATGAAACGCATTAGCACCCCCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCaaa < 1:1508704/149‑1 (MQ=255) cAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGt > 3:167867/1‑149 (MQ=255) gagTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTaa > 1:1525995/1‑149 (MQ=255) cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTaa < 1:228698/144‑1 (MQ=255) cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTaa > 2:228698/1‑144 (MQ=255) acaacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGt < 4:647620/149‑1 (MQ=255) acaacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGC‑tt > 2:1157171/1‑123 (MQ=39) acaacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGC‑tt < 1:1157171/123‑1 (MQ=39) caacaTCCATGAAACGCATTAGCACCACCATTACCTCCACCATCGCCATTTCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTa < 1:356892/149‑1 (MQ=255) aacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTaa > 1:1001962/1‑149 (MQ=255) aacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTaa < 2:1001962/149‑1 (MQ=255) tCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTaacaac > 3:1505545/1‑149 (MQ=255) aTGAAACGCATTAGCACCCCCATTACCACCCCCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGC‑ttttttc < 2:936798/119‑2 (MQ=38) aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑tttttt > 3:799442/1‑118 (MQ=255) aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑tttttt < 4:799442/118‑1 (MQ=255) aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑ttt < 2:585910/115‑1 (MQ=255) aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑ttt > 1:585910/1‑115 (MQ=255) aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGC‑ttttttc > 1:936798/1‑118 (MQ=38) aCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAg > 1:150390/1‑149 (MQ=255) cGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgt < 1:1001318/149‑1 (MQ=255) gCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACa < 1:1320497/97‑1 (MQ=255) gCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACa > 2:1320497/1‑97 (MQ=255) aTTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGtt < 4:310749/149‑1 (MQ=255) ttAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTg > 4:142801/1‑149 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGa < 2:33419/149‑1 (MQ=255) aGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 2:1560097/149‑1 (MQ=255) aGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 2:685257/149‑1 (MQ=255) ccaccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTc > 3:1269101/1‑149 (MQ=255) caccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcg > 3:688988/1‑149 (MQ=255) ccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGGAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc > 4:834384/1‑149 (MQ=255) ccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc > 3:1060528/1‑149 (MQ=255) ccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc < 4:167867/149‑1 (MQ=255) caTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggcgg > 4:1159657/1‑149 (MQ=255) aTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGAAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 3:378601/140‑1 (MQ=255) aTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGAAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa > 4:378601/1‑140 (MQ=255) ttACCACCACCATCACCATTACCACCGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCaaa < 3:1517039/107‑1 (MQ=255) ttACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCaaa > 4:1517039/1‑107 (MQ=255) tACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTa < 2:386737/149‑1 (MQ=255) ccaccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACa > 1:1154018/1‑149 (MQ=255) ccaccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACa > 3:1177684/1‑149 (MQ=255) ccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCa > 4:753803/1‑149 (MQ=255) ccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCa < 1:80604/149‑1 (MQ=255) accatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGt < 3:786962/149‑1 (MQ=255) atTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGc > 3:54295/1‑149 (MQ=255) atTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGC‑tttt < 4:1495985/77‑1 (MQ=25) atTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGC‑tttt > 3:1495985/1‑77 (MQ=25) acaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTaac < 2:1059310/98‑1 (MQ=255) acaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTaac > 1:1059310/1‑98 (MQ=255) acaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACg > 2:1413361/1‑149 (MQ=255) caGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGt < 2:68693/149‑1 (MQ=255) aGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGtt > 1:1357034/1‑149 (MQ=255) ggTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt < 2:663562/149‑1 (MQ=255) gTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGtttt > 1:372141/1‑149 (MQ=255) tAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc < 3:753803/149‑1 (MQ=255) aaCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAAc < 3:193225/84‑1 (MQ=255) aaCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAAc > 4:193225/1‑84 (MQ=255) cGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGc < 1:1555366/149‑1 (MQ=255) gTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgt < 3:889205/149‑1 (MQ=255) gCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTaac > 2:234123/1‑86 (MQ=255) gCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTaac < 1:234123/86‑1 (MQ=255) aCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGata > 2:204610/1‑149 (MQ=255) cgTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTc < 4:688988/149‑1 (MQ=255) aGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaa > 1:1544817/1‑149 (MQ=255) ggAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaaa < 3:1283969/149‑1 (MQ=255) gAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgt > 4:1256380/1‑81 (MQ=255) gAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgt < 3:1256380/81‑1 (MQ=255) gAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAg > 1:865386/1‑149 (MQ=255) aaCACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCa < 3:142801/149‑1 (MQ=255) cacaGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAAt < 1:35896/149‑1 (MQ=255) acaGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACTACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATg > 4:1075685/1‑149 (MQ=255) caGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc > 4:637581/1‑149 (MQ=255) aaaaaGCCCGCA‑CTGTCAGGTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCa < 1:1289414/104‑1 (MQ=38) aaaaaGCCCGCA‑CTGTCAGGTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCa > 2:1289414/1‑104 (MQ=38) ccGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGCCCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGCTATTCTGGAAAGCAATGCCTggcaggggc > 4:168753/1‑149 (MQ=255) ccGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggg > 1:689104/1‑147 (MQ=255) ccGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggg < 2:689104/147‑1 (MQ=255) aCCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGt < 4:1269101/149‑1 (MQ=255) cTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcag < 1:103476/139‑1 (MQ=255) cTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTgg > 2:1392180/1‑149 (MQ=255) gACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGcc > 2:406305/1‑149 (MQ=255) | TAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCC > NC_000913/143‑438 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |