BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,536	G→T	N229K (AAC→AAA)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,536	0	G	T	98.2%	498.9 / NA	164	N229K (AAC→AAA)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base G (0/3); new base T (74/87); total (74/90)
Fisher's exact test for biased strand distribution p-value = 2.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 164 total aligned reads displayed.

TCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGC  >  NC_000913/4116267‑4116807

cggcgcgcccACGGAATGGATCAGCATGCCAGAAACACCGCAACTGCCAACGCATAGTTAACTCCGGCAGCTGGGCCGCATGCGATGGTGTTCAGCAGGCCGGTTTCTGATTTCACCGCTGTCGCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTGCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTc                                                                                                                                                                                                                                                                             <  4:213519/268‑1 (MQ=255)

 cgcgCAGCCACTGAATGGAGGCGACTGCCAGAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTc                                                                                                                                                                                                                                                                             <  3:255818/275‑1 (MQ=255)

   cgcAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCCCAACT‑GACCAAACAGCGCGTCCTGCTGGTCACCCTCGACCCCGTCGCTTGGAATACGCTTGCCGCCTTTCCCCCCACTTTTAGTcgta                                                                                                                                                                                                                                                                          >  4:402130/1‑273 (MQ=255)

   cgcAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGa                                                                                                                                                                                                                                                                          <  2:525637/276‑1 (MQ=255)

         cACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGta                                                                                                                                                                                                                                                                     <  3:395592/275‑1 (MQ=255)

         cACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACGTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGta                                                                                                                                                                                                                                                                     <  1:96096/275‑1 (MQ=255)

                 ggATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGga                                                                                                                                                                                                                                                            <  2:89240/276‑1 (MQ=255)

                  gATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTa                                                                                                                                                                                                                                                                                >  4:449501/1‑255 (MQ=255)

                  gATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGga                                                                                                                                                                                                                                                            >  2:173028/1‑275 (MQ=255)

                  gATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACCTCGga                                                                                                                                                                                                                                                            >  2:188574/1‑275 (MQ=255)

                   aTGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCgg                                                                                                                                                                                                                                                             >  3:437058/1‑273 (MQ=255)

                     gcgcCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTc                                                                                                                                                                                                                                                                             <  2:449501/255‑1 (MQ=255)

                      cgcCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCTTGCCGCCTTTGCCCCCAATTTTAGTCTGACCGTATACTTCGgacga                                                                                                                                                                                                                                                         >  1:449419/1‑274 (MQ=255)

                        ccTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGCTTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGCACGTATACTTCGGAAGAacg                                                                                                                                                                                                                                                      >  2:219169/1‑275 (MQ=255)

                          tGCCATAAACCCCGCACCTCCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAacgacg                                                                                                                                                                                                                                                   <  2:66462/276‑1 (MQ=255)

                                    cccGCACGTTCCCCCCCCTCGTTCACTTCGCCGTCCGGGCCGCAGGCGACGGTTTTCAGCAGGCCCTTTTCTGATTTCACCGCTTTCCCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGgc                                                                                                                                                                                                                                        <  4:267441/275‑1 (MQ=255)

                                      cGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGTAAGAACGACGCA‑CTTCTGgcc                                                                                                                                                                                                                                       >  3:314923/1‑274 (MQ=255)

                                      cGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCCATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGTGCGGCCTGCTGGCCACCGGCGATCCCGTAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGATGAACGATGTA‑CTGCTGtca                                                                                                                                                                                                                                       >  1:280070/1‑275 (MQ=255)

                                            ttCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTACGTCTGACCGTATACTTCGGAAGCACGACGCC‑CTTCTGGCAGAAtct                                                                                                                                                                                                                                 >  1:331003/1‑275 (MQ=255)

                                             cccacagCATAGTTCCATTCGCCAGTCGGGCCGACGGCGATGGTGTTCAGCAGGCCG‑TTTCTGATTTAACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACA‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTcgc                                                                                                                                                                                                                              <  4:455966/270‑1 (MQ=255)

                                               ccaCGCAGAGTTCACTTCGCCAGTCGGGCCGCAGGCTATGTTGGTCAGCCGGCCGTTTTCTGAGTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTcgc                                                                                                                                                                                                                              <  3:552963/273‑1 (MQ=255)

                                               cAACGCATAGTTCACTTCGCCGAGCGGGCCGCAGGCGGTGGTGGTAAACAGGCCGTTTTCTGATTTAACCGCTTTCTCGCCAGTGTTCATCAGCATAAACCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTcgc                                                                                                                                                                                                                              <  3:121924/275‑1 (MQ=255)

                                               cAACGAATAGTTCACTGCGCCGGTCGGGCCGCAGGCCGTGGTGGTCAGCAGGCCGTTTTCGTATTTCACCCCTTTCCCGCCAGTGTTCATCAGCATAAAGCAGCAAGTGCCATAGGTGTTCTTCACCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTcgc                                                                                                                                                                                                                              <  1:644963/275‑1 (MQ=255)

                                                aaCGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCCAAACT‑GACCAAACAGCGCGGCCTGCGGGTCACCGGCGATCCCGGCAAGTGGAATACGCGTGCCGCCTTTACCCCCACCTTTACTCTGGCCGTAACATTCGGAAGAACAGCGCA‑CTTCTGCCACAAtctcccc                                                                                                                                                                                                                             >  4:205148/1‑272 (MQ=255)

                                                   gCATCGTTCCCTTCGCACGTCGCGCCGCATGCGATGGTGGTCTGCAGGCCGTTTTCTGCGTTCACCGCGTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGa                                                                                                                                                                                                                         <  4:638013/276‑1 (MQ=255)

                                                       aGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGCACCA‑CTTCTGGCAGCATCTCGCGCGGAatc                                                                                                                                                                                                                      >  3:517470/1‑274 (MQ=255)

                                                       aGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACCTCGGAAGAACGACGCA‑CTTCTGGCAGCCTTTCGCGCGGAata                                                                                                                                                                                                                      >  2:54636/1‑275 (MQ=255)

                                                         ttCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCACCAGTGTTCATCAGCATAAAGCAGCCAGCGTCATCGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGACCCCGGAGATTTGTATACGCGTGCCACCTTTGCCGCCAATTTTAGTCTGATCGTTAACTTCGGAAGCACCA‑GCATCTTCTCGCACCAtctcccccgtctgat                                                                                                                                                                                                                     >  3:544448/1‑263 (MQ=255)

                                                           cACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTGTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCa                                                                                                                                                                                                                  <  1:495657/275‑1 (MQ=255)

                                                                  ccAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTc                                                                                                                                                                                                           <  2:115564/275‑1 (MQ=255)

                                                                   ctctagcgccccgggCTATTGTGGTAGGCAAGCCCGTTTCTGGATTCACCGCGTTCGCCCCGAGATTCACCAGCATATAGCAGCCGTTGCCATGCGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCa                                                                                                                                                                                                         <  4:59700/263‑1 (MQ=255)

                                                                            cGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgt                                                                                                                                                                                                <  2:569612/276‑1 (MQ=255)

                                                                             gCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAG‑‑‑‑CAGTGCCATAGGTGTTCTTCGCCATCCCTGCCTTCACGCACACCT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTcc                                                                                                                                                                                           <  2:528759/276‑1 (MQ=255)

                                                                                ggCGATGTTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgtcgtc                                                                                                                                                                                            <  2:298447/276‑1 (MQ=255)

                                                                                ggCGATGGTGGTCAGCAGCCCGTTTTCTGATTTCACCGCTTTCCCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATTGGTTTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCNAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgtcgtc                                                                                                                                                                                            <  4:373714/276‑1 (MQ=255)

                                                                                  cGATGGTGGTCAGCTGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTcc                                                                                                                                                                                           <  1:130844/275‑1 (MQ=255)

                                                                                     tggtggTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTcc                                                                                                                                                                                           <  1:709976/272‑1 (MQ=255)

                                                                                       gtggtCAGCGGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCGTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCAGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTc                                                                                                                                                                                      <  2:319450/275‑1 (MQ=255)

                                                                                       gtggtCAGCGAGACGTTGTCTGATTTCCCCGCTTTCTCGCCAGGGTTCATCAACATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTGCTTTCGCGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTc                                                                                                                                                                                      <  2:647111/275‑1 (MQ=255)

                                                                                       gtggtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGACAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTc                                                                                                                                                                                      <  2:202327/275‑1 (MQ=255)

                                                                                       gtggtCAGCAAGCCGGTGTCTGATCTCACCGCATTCGAGCCAGTGTTCATCAGCATAAGGCAGCTAGTGCCAGAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGACTGATGGTCACCGGCGATCACGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCAGCGCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTc                                                                                                                                                                                      <  2:608931/275‑1 (MQ=255)

                                                                                        tggtCAGCAGGCCGTTTTCTGATTTCCCCTCTGTCTTGCCAGACTTCCTCCGCCAAAAGCAGGCAGGGCCATAGGTGTTCGTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTc                                                                                                                                                                                      <  3:499110/274‑1 (MQ=255)

                                                                                        tggcCAGCAGGCCGTTTTCTCATTTCACCGCTTTCGCGCCAGTGGTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑AACCAAACAGCGCGACCTGCTGGTCACCGGCGAGCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGGTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCACAGTc                                                                                                                                                                                      <  1:501703/274‑1 (MQ=255)

                                                                                           tCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGACTATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAgg                                                                                                                                                                                  >  2:586341/1‑275 (MQ=255)

                                                                                               cagGCCCTTTCCTGTTTTCCCCGCGTCCCCGCCAGGGTTCATCAGCATAAAGCAGCCAGTGCCATAGGGGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTAt                                                                                                                                                                              <  4:681796/275‑1 (MQ=255)

                                                                                                  gCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGCATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCCCTGCATATCCAGCACTTCCAGCATTTTGTCCTCCCAGTCCCGGGTCTggc                                                                                                                                                                           >  3:163859/1‑274 (MQ=255)

                                                                                                    cGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATg                                                                                                                                                                         <  3:460772/275‑1 (MQ=255)

                                                                                                    cGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTGCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCTCAGTCCTGGGTATGGATg                                                                                                                                                                         >  1:307362/1‑275 (MQ=255)

                                                                                                      ttttCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGtt                                                                                                                                                                       >  1:323953/1‑275 (MQ=255)

                                                                                                         tCTGAGTTCACCGCTTTCTCTCCAGTTGTCATCAGCATAAAGCAGCCAGTGCCATACGGGTTCTTCGCCATCCCTTATTACCCGCACAACT‑GACCAAGCAGCGCGGCCGGCTGGTCACCGGCGATCCCTAAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGGACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaa                                                                                                                                                                    <  3:250594/275‑1 (MQ=255)

                                                                                                            gATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCCCGCGCGGAATATCCAGCACTTCCAGCATTTTGGCGTCCCAGTCAATGGGCTGGTTGTTGaaca‑a                                                                                                                                                                >  1:282635/1‑275 (MQ=255)

                                                                                                              tttCACCGCTTTCTCCCCACTGTTCCTCAGCCTAAAGCAGCCATCGCCATAGGTTTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACCTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACAt                                                                                                                                                             <  4:188574/276‑1 (MQ=255)

                                                                                                                       tttCTCTCCACTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCGTCTTTCACGCACAATT‑GACCAACCAGCGCTGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACgag                                                                                                                                                     <  1:353761/275‑1 (MQ=255)

                                                                                                                                    ttCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTa                                                                                                                                        >  3:389143/1‑275 (MQ=255)

                                                                                                                                            cATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATCCTTCGGAAGAACGACGCC‑CTTCTGGCAGCATCCCGCGCGGACTATCCAGCACTTCCCGCATTTTGTCGTCCCAGTCCAGGGTATGGCTGTTGAACA‑ACATGGTACGACAGGCGTTGGTGTAATCggcc                                                                                                                                 >  2:236398/1‑272 (MQ=255)

                                                                                                                                              tAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGGTGGTGTAATCGGTcaca                                                                                                                              >  1:106212/1‑275 (MQ=255)

                                                                                                                                                  gcagcCCGTCCCAAAGGTGTTCGTGGCCATCCCTCCTTTCACGC‑CAACTGGACCAAAAAGCGCGGCCGGCTGGTCACCGGCGATCACGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTAGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGac                                                                                                                         <  4:224836/276‑1 (MQ=255)

                                                                                                                                                    agcacgTGGCATAGGTGTTCTTCGGACTACCTTCTTTCACGTCCAAAT‑GAGCAAACAGCGCGGACTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCGGAACGTATAATGCGGGAGAACGACGCG‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCGAGGGTATGGGTGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAGTCGGTCACATGGaca                                                                                                                        <  3:717020/270‑1 (MQ=255)

                                                                                                                                                    agcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGaca                                                                                                                        <  1:575372/275‑1 (MQ=255)

                                                                                                                                                    agcCAGTACCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACG‑GAACAAGCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGAA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGaca                                                                                                                        <  1:83668/275‑1 (MQ=255)

                                                                                                                                                    acccAGTGCCCTAGGTGTTCTTCGACATCCCTGCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGACAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGaca                                                                                                                        <  1:188465/273‑1 (MQ=255)

                                                                                                                                                     gcCCGTGCCCTTGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GCCCAACCAGCGCGCCCTGTTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGacac                                                                                                                       <  3:351280/275‑1 (MQ=255)

                                                                                                                                                                    gtTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGCTTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGCACGAAGCA‑CTCCTGGCCGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCCTCCCCGTCCAGGTCATGGA‑GTTGAACATACCTGGTACGAGAGGCGTTGGTGTAATCGGTAACATGGACACGGcctcgtgccatt                                                                                                         >  3:623744/1‑264 (MQ=255)

                                                                                                                                                                    gtTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCAttt                                                                                                        >  3:540399/1‑275 (MQ=255)

                                                                                                                                                                      tcttcGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACgaga                                                                                                                                                    >  1:400681/1‑229 (MQ=255)

                                                                                                                                                                       cttcGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTcc                                                                                                     >  1:158942/1‑275 (MQ=255)

                                                                                                                                                                           gCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGAt                                                                                                 <  1:437058/275‑1 (MQ=255)

                                                                                                                                                                             cATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGCTGTTGAACA‑AAATGGTACGTGAGGCGTTGGGGTAATCGGGAACATGGACCCGGCACTGAGCCATTTGCAAGagtag                                                                                              >  4:634725/1‑272 (MQ=255)

                                                                                                                                                                               tcccctagtccccGCACAAAT‑GACCAACCAGCGCGGACCGCTGGTCACCGGGGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGGCTGACAGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCAGCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGGCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGc                                                                                             <  4:539771/263‑1 (MQ=255)

                                                                                                                                                                               ccccTTCTTTCACGAACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGc                                                                                             <  2:402130/274‑1 (MQ=255)

                                                                                                                                                                               ccccTTCGTTCCCGCACAACG‑TACCAAACACCGGGGCCTGCTGGTCCCCGGCGATCCCGGGGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGc                                                                                             <  4:586341/274‑1 (MQ=255)

                                                                                                                                                                                cccTTCTTGAACAAATAACT‑GACCAAACAGCGCGGCCTGCTAGTCACCGGCGAACCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGGCTGACCGTATACTACGGAAGAACGACGCA‑CTTCTGGCAGAATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCAAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGc                                                                                             <  2:345002/274‑1 (MQ=255)

                                                                                                                                                                                 ccTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGt                                                                                                                                  <  1:100415/236‑1 (MQ=255)

                                                                                                                                                                                        tCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCaa                                                                                   <  2:198671/276‑1 (MQ=255)

                                                                                                                                                                                        gcccGCACAACT‑GCCCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCaa                                                                                   <  2:25148/273‑1 (MQ=255)

                                                                                                                                                                                           cGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGCGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAAcc                                                                                 >  1:495917/1‑275 (MQ=255)

                                                                                                                                                                                           cGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTATGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑CCATGGTACGAGAGGCGTTGGTGTAATCGGTTACATGGACACGGCCCTGAGTCATTTTCAAGATAAGACACGGATCAACCg                                                                                >  2:99798/1‑276 (MQ=255)

                                                                                                                                                                                                  cT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTT‑AACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAAc                                                                        <  2:435976/276‑1 (MQ=255)

                                                                                                                                                                                                  cT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCCCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACCAGGCCCTGAGTCATTTTCCAGATAAGCCCCGTATCAATCGTACCa                                                                           >  1:671396/1‑274 (MQ=255)

                                                                                                                                                                                                           aCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTAGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGGGTTGGTGTAATAGGTCACATGGACACGGCCCTGAGTCAGTTTCCAGATAAGCCACGTATCAACCGTAGCAAACAGCAAt                                                                  >  1:644882/1‑275 (MQ=255)

                                                                                                                                                                                                               cgcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGGTGGTGTAATCGGTCACATTGACACGGCCCTGGGTCATTTTCCAGTTAAGCCACGGCTAAACCGTACCAACAAGCAAttaaa                                                              >  3:641967/1‑272 (MQ=255)

                                                                                                                                                                                                                         tgggccccGGCGATCCCGTCGATTGGAATACGCGTGCCGCCTTTGCAGCCAATTTTAGTCTGCCCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATGAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCa                                                   <  4:219169/270‑1 (MQ=255)

                                                                                                                                                                                                                            tCACCGGAGTTAACGGAGAGTGGAATACTCTTGCCGCAGTAGCCGCCAATTTTAGTCTGAACGTATACTTCGGAAGAACGCAGGA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTGCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACg                                                 <  2:173320/275‑1 (MQ=255)

                                                                                                                                                                                                                                cGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaaca‑ac                                                                                                                                                               >  3:566844/1‑161 (MQ=255)

                                                                                                                                                                                                                                cGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCACATTCCAGTGTATGGAGGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGTTCACATGGACACGGCCCTGAGTCATTTTCCAGTTAAGCAACGTATCAACTGAACCAAACAGAAGTTCACCACGACGtcccccctc                                              >  3:22672/1‑266 (MQ=255)

                                                                                                                                                                                                                                    gATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATgg                                                                                                                                                           <  1:566844/161‑1 (MQ=255)

                                                                                                                                                                                                                                           gagaTTGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTGGAACC‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACCCGGCCCTGAGGTATTTTCCATATAAGCCACGTATCACCACTACCAAACAGCAATTCACCACGACGTGAACGCTCGCGAGAGCTTTc                                  >  3:162233/1‑275 (MQ=255)

                                                                                                                                                                                                                                                tGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCCGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTACTCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCCCGACGTGCACCCTCGCGAGAGCATTCcaca                              >  3:305356/1‑274 (MQ=255)

                                                                                                                                                                                                                                                tGGAATACGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCCAGTATCAACCGTCCCAAACAGCAATTCACCACGACGTGCACGCTCGCGACAGCCTTCccca                              >  2:59052/1‑274 (MQ=255)

                                                                                                                                                                                                                                                         cgTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaac                                                                                                                                                                   >  3:458929/1‑133 (MQ=255)

                                                                                                                                                                                                                                                         cgTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGa                    >  1:478530/1‑275 (MQ=255)

                                                                                                                                                                                                                                                           tgctgcCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCTCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATc                  <  2:80342/275‑1 (MQ=255)

                                                                                                                                                                                                                                                           tGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAAcc                                                                                 >  1:582772/1‑212 (MQ=255)

                                                                                                                                                                                                                                                            gccgccTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACAAAACAGCAATTCAACCCGACGTGAACACCCGCAACAGCCTTCCCCATGCTCGCGGaaca                 >  4:311198/1‑272 (MQ=255)

                                                                                                                                                                                                                                                             ccgccTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAaca‑aca                                                                                                                                                              <  1:458929/133‑1 (MQ=255)

                                                                                                                                                                                                                                                             ccgccTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCCTTTTCCAGATAAGCCACGCATCAACCGTACCAAACAGCAATTCACCACGACTTGCCCGCTCGCGACAGCCTTCCACATggaccatgaccc                 >  3:719029/1‑264 (MQ=255)

                                                                                                                                                                                                                                                             ccgccTTTGCCGCCAATTTTAGTCTGACCGTATACTTAGGCAGAACGACGCC‑CTTCTGGCAGCATCTCGCGCGGAATATCAAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGTACA‑ACATGGTACGCGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCAAGATCAGACTCGTATCAACCGTACCCACAAGCCATTCACCACGAAGTGCACGCTCGCGAGTGCCTTCCCCATGGTCGAGGATCca                >  2:130937/1‑275 (MQ=255)

                                                                                                                                                                                                                                                               gccTTTGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTAc                                                                             <  3:582772/212‑1 (MQ=255)

                                                                                                                                                                                                                                                                  tttGCCGCCAATTTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTTCACCCTCGCGAGGGCCTTCCACATGGTCGAGGAGCcacctcac          >  2:604606/1‑276 (MQ=255)

                                                                                                                                                                                                                                                                          cAATTTTCGTCTGCCCGTTTATTTCGCAAGAACGCCCAC‑CTTCTGGCAGCATCTCGCGCCGAATATCCAGCACTTCCATCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGc  <  4:604606/276‑1 (MQ=255)

TCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACT‑GACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCA‑CTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACA‑ACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGC  >  NC_000913/4116267‑4116807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`