| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 657,531 | T→G | 18.2% | intergenic (+30/+24) | cspE → / ← flc | constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 657,531 | 0 | T | G | 18.2% | 324.8 / 74.3 | 139 | intergenic (+30/+24) | cspE/flc | constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (45/68); new base G (9/16); total (55/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.59e-01 | |||||||||||
Only 100 of 144 total aligned reads displayed.
TCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAA > NC_000913/657432‑657630 | tCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCtt > 2:1226163/1‑100 (MQ=255) gcgcGTAGAGTTCGAAATCACTAACGGTGCCAGAGGCCCTTCTGCTGCCAACGTAATCGCTCTGTAAGATACGTCAGCAAGTATTCAAAACCCGCTTAAt > 1:1714532/1‑100 (MQ=255) gcgcGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAAt > 1:1022346/1‑100 (MQ=255) cgcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATc < 2:644760/100‑1 (MQ=255) cgcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATc > 2:3080561/1‑100 (MQ=255) cgcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATc < 2:2733550/100‑1 (MQ=255) gTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGc < 1:1682542/100‑1 (MQ=255) tAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg < 2:1159038/100‑1 (MQ=255) tAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg < 1:127844/100‑1 (MQ=255) gagTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCggg < 1:3789634/100‑1 (MQ=255) gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCggg > 2:1865071/1‑98 (MQ=255) gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑tt > 1:683554/1‑100 (MQ=255) gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑tt < 1:10279/100‑1 (MQ=255) gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑tt > 1:3456325/1‑100 (MQ=255) tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑tttt < 1:3799591/100‑1 (MQ=255) tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑tttt < 2:900531/100‑1 (MQ=255) tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑tttt < 1:797300/100‑1 (MQ=255) tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑tttt < 1:308396/100‑1 (MQ=255) cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGNG‑ttttt > 1:3419226/1‑100 (MQ=255) cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑ttttg < 1:3910050/100‑2 (MQ=39) cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑ttttg < 2:2238136/100‑2 (MQ=39) cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCAATCTGTATGCTCCGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑ttttt > 2:1492627/1‑100 (MQ=255) gAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑tttttt < 1:1826265/100‑1 (MQ=255) gAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑ttttga < 1:138547/100‑3 (MQ=38) gAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑ttttga < 1:322017/100‑3 (MQ=38) aaaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑ttttttt < 1:3080581/100‑1 (MQ=255) aaaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑ttttgaa < 2:1472467/100‑4 (MQ=38) aaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAGAACCCGCTTAATCAGCGGG‑tttttttt < 1:231812/100‑1 (MQ=255) aaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑ttttgaat < 1:144876/100‑5 (MQ=38) aaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑ttttgaat < 2:2007093/100‑5 (MQ=38) aTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTg < 2:2823376/100‑1 (MQ=255) tCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTgg < 1:454296/100‑1 (MQ=255) tCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTgg < 1:2736469/100‑1 (MQ=255) tCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTgg < 2:3942091/100‑1 (MQ=255) aCTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTc > 2:3048953/1‑100 (MQ=255) atAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTACTCGCTCTGTAAGATACATCGGCCAGAACTCAGAACCCGCTTACTCAGCGGG‑TATTTTTCGGt > 2:708266/2‑98 (MQ=255) aaCGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCttt < 2:2252758/100‑1 (MQ=255) aaCGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCttt < 2:1415138/100‑1 (MQ=255) cGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAg < 1:3081155/100‑1 (MQ=255) aaaGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCgg < 2:3700195/100‑1 (MQ=255) aaGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGt < 1:3322684/100‑1 (MQ=255) aGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGtt < 2:348410/100‑1 (MQ=255) ggCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTg < 1:1092857/100‑1 (MQ=255) gCCCTTCTGCTTCAAACGTAGTCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGt < 2:2685327/97‑1 (MQ=255) ccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAgg < 2:1430036/100‑1 (MQ=255) cTTCTGCTGCAAACGTAATTGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGc < 1:405086/100‑1 (MQ=255) cTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGc > 1:2344430/1‑100 (MQ=255) ttCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTTTTTAGTGTGCGGTTGAGGcc < 2:2412312/100‑1 (MQ=255) ttCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGcc < 1:3604342/100‑1 (MQ=255) tgctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaa < 2:2448450/100‑1 (MQ=255) gctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaaa < 2:1596893/100‑1 (MQ=255) ctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaaaa < 2:381899/100‑1 (MQ=255) gcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGAGGG‑TTGTTTTTGTTCTTTAGTGTGCGGTTGAGGCAGAAAAc > 2:3658763/1‑99 (MQ=255) cAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGTG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACa < 2:3759926/99‑1 (MQ=255) cAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACa < 2:2472110/99‑1 (MQ=255) aCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCa < 2:104125/100‑1 (MQ=255) cGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAg > 1:1001416/1‑100 (MQ=255) gTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTATGGTCTTTAGTGTGCGGTTGAGGCTGAAAACAGCCAGa < 2:2434401/100‑1 (MQ=255) tAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGaa > 1:3863623/1‑100 (MQ=255) cGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGcc > 2:2339452/1‑100 (MQ=255) ctctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAg < 1:1672482/100‑1 (MQ=255) ctctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAg < 1:3846883/100‑1 (MQ=255) ctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTg > 1:1249082/1‑100 (MQ=255) ctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTg < 1:257284/100‑1 (MQ=255) gTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTTTTTAGTGTGCGATTGAGGCCGAAAACAGCCAGAATGCCAGTGCg > 2:887472/1‑100 (MQ=255) gTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCg > 1:2507299/1‑100 (MQ=255) aaGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGt < 2:3456302/100‑1 (MQ=255) aaGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGt > 1:1409331/1‑100 (MQ=255) tACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATg < 2:1016021/100‑1 (MQ=255) tACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATg < 1:710980/100‑1 (MQ=255) cGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATgg < 2:1079059/99‑1 (MQ=255) cGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGc < 1:3310506/100‑1 (MQ=255) gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGAG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCa < 1:3290491/100‑1 (MQ=255) cAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaa > 1:370833/1‑100 (MQ=255) aGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaaa > 1:2703600/1‑100 (MQ=255) gCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTTAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg > 2:2921740/1‑100 (MQ=255) gCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg > 1:3231258/1‑100 (MQ=255) gCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg < 1:2251186/100‑1 (MQ=255) aaGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGa > 1:121333/1‑100 (MQ=255) aaGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAc < 2:3843642/100‑1 (MQ=255) aGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcc > 1:643805/1‑100 (MQ=255) ccaaaaaaaaCCCGCTGATTAAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAccc > 2:137942/7‑100 (MQ=37) aaTTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc < 1:1044127/100‑1 (MQ=255) aaTTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc > 2:2735151/1‑100 (MQ=255) aaaaaaaaCCCGCTGATTAAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCATAATGCCAGGGCGGTCATGGCAAAAGACCCCa > 2:2657973/5‑100 (MQ=37) aaaaaaaaCCCGCTGATTAAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCa > 1:1322015/5‑100 (MQ=38) aaaaaaaaCCCGCTGATTAAGCGGG‑TTTTGTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCa > 2:405052/5‑100 (MQ=38) aTTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTTTTTTTTTTTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAccc > 2:3008354/1‑98 (MQ=255) aTTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCa > 1:3364551/1‑100 (MQ=255) aaaaCCCGCTGATTAAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAg > 1:711891/1‑100 (MQ=255) aaCCCGCTTAATCAGCGGG‑TTTGTTTTGGTCTTTAGGGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGt < 2:1274152/100‑1 (MQ=255) aaCCCGCTGATTAAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCGAGTGCGGTCATGGCAAAAGACCCCAGa > 2:3330221/1‑96 (MQ=255) aCCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGtt > 2:2639573/1‑100 (MQ=255) aCCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTCAGGCCGAAAGCAGCCAGAATGCCAGTGCGGTCATGGCGAAAGACCCCa > 2:2303551/1‑93 (MQ=255) cccGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTg < 2:1695921/100‑1 (MQ=255) ccGCTTAATCAGCGGG‑GTTTTTTTGGGCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGt < 2:2358724/97‑1 (MQ=255) gCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACg > 1:3430832/1‑100 (MQ=255) gCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACg < 1:2191023/100‑1 (MQ=255) gCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACg < 1:1718238/100‑1 (MQ=255) tAATCAGCGGG‑TTTTTTTTTGTCTTTAGTTTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTCACGaaa > 2:3264292/1‑100 (MQ=255) | TCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAA > NC_000913/657432‑657630 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |