BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,781,152	0	A	G	20.2%	214.2 / 22.9	110	intergenic (+135/‑63)	lldD/trmL	L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base A (59/28); new base G (0/22); total (60/50)
Fisher's exact test for biased strand distribution p-value = 1.46e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 111 total aligned reads displayed.

GGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCG  >  NC_000913/3781053‑3781250

gggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGa                                                                                                      >  2:2656521/1‑100 (MQ=255)

   agagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑ggg                                                                                                  <  1:2656533/100‑1 (MQ=255)

    gagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑gggg                                                                                                 <  2:1138473/100‑1 (MQ=255)

    gagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGAGA‑gggg                                                                                                 >  1:2793143/1‑100 (MQ=255)

      ggggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTTTTTTTTT‑CTCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑g                                                                                                    >  2:559493/1‑95 (MQ=255)

        ggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑aaaa                                                                                            >  1:1872188/1‑100 (MQ=255)

          aCCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAgg                                                                                          <  1:3953/100‑1 (MQ=255)

           ccGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AACAGGc                                                                                         >  2:1263107/1‑100 (MQ=255)

             gTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCg                                                                                       >  1:1277331/1‑100 (MQ=255)

                 ggCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACt                                                                                   >  1:472856/1‑100 (MQ=255)

                 ggCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGCGA‑GGGG‑AAAAGGCCGCACt                                                                                   >  1:1644918/1‑100 (MQ=255)

                      tttatgtcCCCCCTCCCCCTAAAACGACCCCGCCCTTTTTTTTCCCCCCCCG‑CCCCCCG‑GGGGGGGGGGCG‑GGGGGGG‑GGGG‑AAAAGGCCGCACTGCt                                                                                <  1:844344/96‑1 (MQ=255)

                         atgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCa                                                                           >  2:2170817/1‑100 (MQ=255)

                          tgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCaa                                                                          >  2:1386601/1‑100 (MQ=255)

                            taCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGC‑GGGG‑AAAAGGCCGCACTGCTCACCAAtt                                                                        >  2:1060303/1‑100 (MQ=255)

                            taCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAAtt                                                                        >  1:2573514/1‑100 (MQ=255)

                            taCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAAtt                                                                        >  1:1026559/1‑100 (MQ=255)

                            taCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAAtt                                                                        >  1:1633261/1‑100 (MQ=255)

                              cccccTCACTCGGAACCGACCCCGCCTTTTTTTTTTCCCCCCCG‑CCCCCCG‑GGGGGGGGGGCG‑GGGGGGG‑GGGG‑AAAAGGCCGCACTGCTCACCAAttt                                                                       <  1:2245156/97‑1 (MQ=255)

                                ccccccccctGAAACGACACCGCCCTCTTTTTTTCCCCCCCC‑CCCCCCG‑GGGGGGGGGGCC‑GGGGGGA‑GGGG‑AAAAGGCCGCACTGCTCACCAAttt                                                                       <  2:1420068/89‑1 (MQ=255)

                                cccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAAtttaga                                                                    >  2:2245145/1‑97 (MQ=255)

                                cccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCt                                                                    >  2:686953/1‑100 (MQ=255)

                                 ccTCACTCTGAAACGACACCGCACTCTTTTTTTTTTTTTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTg                                                                   >  2:1768637/1‑100 (MQ=39)

                                 ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTg                                                                   >  1:242813/1‑100 (MQ=255)

                                  cTCCCTCTGAAACGACCCCGCCCTCTTTTTTTCCCCCGCG‑CCCCCCG‑GGGGGGGGGGCG‑GGGGGGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGc                                                                  <  2:1107608/100‑1 (MQ=255)

                                   cccctctGAAACGACCCCGCCCTGTTTTTTTCCCCCGCG‑CCCCCCG‑GGGGGGGGGGCG‑GGGGTGG‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGc                                                                  <  2:296152/96‑1 (MQ=255)

                                    ccctctGAACCGACCCCGACCTCTTTTTTTCTCCCTCG‑CCCC‑CC‑GGGGGGGGGGCCGGGGGTGG‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGc                                                                  <  2:1277326/96‑1 (MQ=255)

                                    cccgcagacacgacgCGGCACTCTTTTTTTCCCCCCCG‑CCCCCCG‑GGGGAGGGGGCG‑GGGGGGG‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGc                                                                  <  2:2021154/89‑1 (MQ=255)

                                    cccccgcaaaCGACACCGCACTCTTTTTTGCCCCCCCC‑CCCCCCG‑GGGGGGGGGGCC‑GGGG‑GA‑GGGGAAAAAGGCCGCACTGCTCACCAATTTTCTg                                                                   <  2:1221690/90‑1 (MQ=255)

                                     cctctGAACCGCCGCCGACCTTTTTTTTTCTCCCCCG‑CCCCCCG‑GGGGGGGGGGCG‑GGGGTGG‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGc                                                                  <  2:410429/96‑1 (MQ=255)

                                     aCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTAt                                                               >  1:1663654/1‑100 (MQ=255)

                                      ctctGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATc                                                              >  2:672352/1‑100 (MQ=255)

                                      ctctGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATc                                                              >  2:721565/1‑100 (MQ=255)

                                        ctGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                            >  2:2533324/1‑100 (MQ=255)

                                        ctGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                            >  1:2027178/1‑100 (MQ=255)

                                        ctGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                            >  1:616035/1‑100 (MQ=255)

                                         tcaaaCGACCCCGCACTCTTTTTGTGGCCCTCC‑CCCCTCC‑GGTGTGAGGCTG‑GGGTTGA‑GGGG‑GAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                           <  2:580173/98‑1 (MQ=255)

                                         tGAAACGACACCGCACTCTTTTTTTTTTTTTTT‑TTCCCCC‑GGGGAGAGGGCC‑GCGGTGA‑TGAG‑AAAAGGCCGCAATGCTCACCAATTTTCTGCTATcc                                                             >  2:70124/1‑98 (MQ=255)

                                          gAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGc                                                          >  2:319492/1‑100 (MQ=255)

                                           aaaCGACCCCGCACTCTTTTTTCCTCCCCCG‑CCCCTCC‑GGGGAGGGGGCC‑GGGGTGG‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                             <  2:1750015/96‑1 (MQ=255)

                                           aaaCGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcc                                                         >  1:2703994/1‑100 (MQ=255)

                                            accGACCCCACACTTTTTTTTTCCCCCGCC‑CCCCTCC‑GGGGAGAGGGCG‑GGGGTGA‑GGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                             <  2:750495/93‑1 (MQ=255)

                                            aaCGACCCCGCACTTTTTTTTCCTCCCCCC‑CCCCTCG‑GGGGAGAGGGCC‑GGGGGGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                           <  2:763045/97‑1 (MQ=255)

                                            aaCGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc                                                        >  1:2080073/1‑100 (MQ=255)

                                            aaCGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc                                                        >  2:2609733/1‑100 (MQ=255)

                                             acgcccccGCCCTTTTTTTTCCCCCCCCC‑CCCCCCC‑GGGGGGTGGGCC‑GGGGTGG‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                                       <  1:357909/94‑1 (MQ=255)

                                              cgccacCGCCCTCTTTTTTCGTCCCTCT‑CCCCCCC‑GGGGAGAGGCCG‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                           <  2:579933/92‑1 (MQ=255)

                                              cGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc                                                        <  2:2635848/99‑1 (MQ=255)

                                                accccccACTTTTTTTTTCCCCCCCC‑CCCCTCG‑GGGGAGAGGGCG‑GGGTTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                                                      <  1:219917/92‑1 (MQ=255)

                                                 ccccGCATTCTTTTTTCCCCCCGCG‑CCCCGCG‑GGGGGGGGGCCG‑GGGGTGG‑GGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                                                      <  1:721568/95‑1 (MQ=255)

                                                 cacCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCa                                                   >  2:1423306/1‑100 (MQ=255)

                                                 cacCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCa                                                   >  1:1224808/1‑100 (MQ=255)

                                                  cccGCCCTTTTTTTTGCCCCGCCGCCCCCCCC‑GGGGAGAGTCCC‑GGGTGGG‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                                       <  2:1279923/95‑1 (MQ=255)

                                                   ccccACTCTTTTTTCCCCCGCCC‑CCCCGCG‑GGGGGGGGGGCG‑GGGGTGG‑GGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                                 <  2:456659/97‑1 (MQ=255)

                                                   ccGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                                 >  1:1073080/1‑100 (MQ=255)

                                                   ccGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                                 >  2:1439958/1‑100 (MQ=255)

                                                       cccttttttttCCCCCCCC‑CCCCCCCGGGGGGGGGGGCG‑GGGGTGG‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGc                                                    <  2:2027168/90‑1 (MQ=255)

                                                       aCTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACCAAgg                                             >  2:2726328/1‑100 (MQ=255)

                                                         tttttttgtCTCCCGTG‑CCCCCCT‑GGGGAGGGGCCC‑GGGGTGG‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                                     <  2:1813474/88‑1 (MQ=255)

                                                          ttttttttCCCCCCCC‑CCCCCCCGGGGGAGGGGGCC‑GGGTTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                           <  1:622060/99‑1 (MQ=255)

                                                          ctTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                          >  1:2781667/1‑100 (MQ=255)

                                                          ctTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                          >  2:830674/1‑100 (MQ=255)

                                                                ttCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt                                    >  1:1419972/1‑100 (MQ=255)

                                                                   tccgatctccccTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc                                  <  1:819167/92‑1 (MQ=255)

                                                                    cccTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                                >  2:1349388/1‑100 (MQ=255)

                                                                    cccTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                                >  1:2504590/1‑100 (MQ=255)

                                                                     ccTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                               >  1:2387502/1‑100 (MQ=255)

                                                                     ccTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                               >  1:590429/1‑100 (MQ=255)

                                                                      cccccccccccC‑GGGGAGGGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc                                  <  2:597297/92‑1 (MQ=255)

                                                                         ccccccGCC‑GGGGGGGGGGCC‑GGGGGGG‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAAc                             <  1:1060307/97‑1 (MQ=255)

                                                                           ccccTCG‑GGGGGGGGGGCG‑GGGGTGG‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACAt                           <  2:2014674/99‑1 (MQ=255)

                                                                           ccccTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGcagatcgga                          >  2:819164/1‑92 (MQ=255)

                                                                           ccccTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc                          >  1:681659/1‑100 (MQ=255)

                                                                           ccccTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc                          >  1:1216055/1‑100 (MQ=255)

                                                                           ccccTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc                          >  2:1250759/1‑100 (MQ=255)

                                                                            cccTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg                         >  1:2037760/1‑100 (MQ=255)

                                                                            cccTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg                         >  1:2296001/1‑100 (MQ=255)

                                                                              cTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                       >  1:2348576/1‑100 (MQ=255)

                                                                               tCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc                      >  2:1349124/1‑100 (MQ=255)

                                                                                cggggggggggggCG‑GGGGGGG‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc                      <  1:1455314/97‑1 (MQ=255)

                                                                                 cgggggggAGGGCC‑GGGGGGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc                      <  2:2137581/97‑1 (MQ=255)

                                                                                  cgggggggcggCC‑GGGGGGG‑GGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc                      <  1:118320/90‑1 (MQ=255)

                                                                                    ggggcgggcac‑GGGGGGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGTGGGCAGTATGCTAAACATCGTACTTTa                  <  1:987079/90‑1 (MQ=255)

                                                                                    gggcgagGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTa                  <  2:311291/96‑1 (MQ=255)

                                                                                     ggggggggCC‑GGGGGGA‑GGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                 <  1:1608249/95‑1 (MQ=255)

                                                                                     ggAGGGGGCC‑GGGGTGG‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                 <  2:1872179/100‑1 (MQ=255)

                                                                                     ggAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                 >  1:1553536/1‑100 (MQ=255)

                                                                                     ggAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                 >  2:1382050/1‑100 (MQ=255)

                                                                                      gggggggCG‑GGGGGGG‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg                <  1:830677/96‑1 (MQ=255)

                                                                                      gagaGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg                >  2:2543491/1‑100 (MQ=255)

                                                                                          gggCG‑GGGGGGG‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc            <  1:2057752/100‑1 (MQ=255)

                                                                                          gggCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc            >  2:1263436/1‑100 (MQ=255)

                                                                                          gggCC‑GGGGTGA‑GAGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc            >  2:1738361/1‑100 (MQ=255)

                                                                                          gggCC‑GGGGGGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAc             <  1:1007501/99‑1 (MQ=255)

                                                                                                ggggTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaaa       >  2:535876/1‑100 (MQ=255)

                                                                                                ggggTAG‑GGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa         <  1:324279/98‑1 (MQ=255)

                                                                                                  ggTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAAt      <  2:1026555/99‑1 (MQ=255)

                                                                                                    tGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTc    >  1:2534838/1‑99 (MQ=255)

                                                                                                     gA‑GGGG‑AAAATGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCg  <  2:1847135/100‑1 (MQ=255)

                                                                                                     gA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCg  <  1:1349130/100‑1 (MQ=255)

GGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCG‑CCCCTCC‑GGGGAGAGGGCC‑GGGGTGA‑GGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCG  >  NC_000913/3781053‑3781250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`