| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,781,152 | 0 | A | G | 27.9% | 99.2 / 26.6 | 61 | intergenic (+135/‑63) | lldD/trmL | L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (37/7); new base G (0/17); total (37/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.45e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAG > NC_000913/3781058‑3781242 | cgatctccGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGa < 2:1708417/94‑1 (MQ=255) ggggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGaa > 2:891503/1‑100 (MQ=255) ggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGaaaa > 2:1472163/1‑100 (MQ=255) ggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGaaaa > 2:1028415/1‑100 (MQ=255) cGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCAc > 1:309349/1‑100 (MQ=255) cGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCAc > 2:309349/1‑100 (MQ=255) gcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCt > 1:205159/1‑100 (MQ=255) gcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCt > 2:205159/1‑100 (MQ=255) cTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTc > 2:428726/1‑100 (MQ=255) cTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTc > 1:428726/1‑100 (MQ=255) tgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCaa > 2:780748/1‑100 (MQ=255) tgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCaa > 1:780748/1‑100 (MQ=255) tgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTATTTCTCCCTCGCCCCTCC‑GGGGAGCGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCa > 2:1810096/1‑99 (MQ=255) tgcccccccTCACCCGGAAAGGCCACCCCCCTCTTTTTTCCCCCCTCCCCCCGCG‑GGGGAGGGGGCC‑GGGGTGGGGGGAAAAGGCCGCACTGCTCAcc < 2:167412/92‑1 (MQ=255) tgcccccccTCACCCGGAAAGGCCACCCCCCTCTTTTTTCCCCCCTCCCCCCGCG‑GGGGAGGGGGCC‑GGGGTGGGGGGAAAAGGCCGCACTGCTCAcc < 1:167412/92‑1 (MQ=255) cctccctccCTCTGAAACGCCCCCGCACTTTTTTTTTCTCCCCCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAttt < 2:2296283/99‑1 (MQ=38) cTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtttt > 2:1576898/1‑100 (MQ=255) cccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCt > 2:1820111/1‑100 (MQ=255) cccccctcgaaaaCGACCCGCCCCTCTTTTTTTCCCCCGC‑CCCCCCC‑GGGGGGGGGGCCGGGGGGGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg < 2:263559/90‑1 (MQ=255) cccccctcgaaaaCGACCCGCCCCTCTTTTTTTCCCCCGC‑CCCCCCC‑GGGGGGGGGGCCGGGGGGGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg < 1:263559/90‑1 (MQ=255) ccTCACTCGGAAACGACACCGCCCTTTTTTTTCCTCCCCCCCCCCTCC‑GGGGAGGGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg < 2:1766470/100‑1 (MQ=255) cccACTCTAAAACGCCCCCGC‑CTTTTTTTTTCCCCCCCCCCCCCCCGGGGGGGGGGGCG‑GGGGGGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGc < 2:1265468/98‑1 (MQ=255) tgaaaaCGCCCCCGCCCTCTTTTTTTCCCCCCCCCCCCCCC‑GGGGAGGGGCCC‑GGGGGGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc < 2:1708221/96‑1 (MQ=255) tGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg > 2:1910780/1‑100 (MQ=255) tGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg > 2:922238/1‑100 (MQ=255) gaaaaCGACCCCCCCCCTTTTTTTCCCCCCCCGCCCCGCG‑GGGGAGGGGGCC‑GGGGTGGGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc < 2:2099558/96‑1 (MQ=255) aaaCGACCCCGCACTCTTTTTTCCCCCCTCCCCCCTCG‑GGGGAGGGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcc < 2:1460971/100‑1 (MQ=38) acacCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGc > 2:2190603/1‑100 (MQ=255) ttttttttttCCCCCTCCCCCCCCG‑GGGGAGGGGGCG‑GGGGGGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg < 2:491764/97‑1 (MQ=255) ttttttttttCCCCCTCCCCCCCCG‑GGGGAGGGGGCG‑GGGGGGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg < 1:491764/97‑1 (MQ=255) ctctTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg > 2:1890201/1‑100 (MQ=255) cctTTTTTTTCCCCCCCCCCCCCC‑GGGGGGGGGGCCGGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg < 2:2195760/98‑1 (MQ=255) tctcCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCCCACTAAGGGGGCAGTATg > 1:249971/1‑100 (MQ=255) tctcCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCCCACTAAGGGGGCAGTATg > 2:249971/1‑100 (MQ=255) cccgccccccGCG‑GGGGGGGGGGCG‑GGGGGGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt < 2:2093499/90‑1 (MQ=255) ccTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa > 1:206711/1‑100 (MQ=255) ccTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa > 2:206711/1‑100 (MQ=255) ccTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa > 2:2085417/1‑100 (MQ=255) cGCCCCGCG‑GGGGGGGGGGCG‑GGGGGGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACa < 2:590876/100‑1 (MQ=255) cGCCCCGCG‑GGGGGGGGGGCG‑GGGGGGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACa < 1:590876/100‑1 (MQ=255) gCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACAt > 2:1645597/1‑100 (MQ=255) ccccTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc > 2:2232980/1‑100 (MQ=255) ccccTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc > 2:2009404/1‑100 (MQ=255) ccccTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc > 2:2232914/1‑100 (MQ=255) cgccgggggggggggCG‑GGGGTGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg < 2:410264/94‑1 (MQ=255) cgccgggggggggggCG‑GGGGTGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg < 1:410264/94‑1 (MQ=255) gggggggggggGC‑GGGGGGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACt < 2:1834530/98‑1 (MQ=255) ggggAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt > 2:1911163/1‑100 (MQ=255) ggggggggCG‑GGGGGGGGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc < 2:2629/95‑1 (MQ=255) ggggggggCG‑GGGGGGGGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc < 1:2629/95‑1 (MQ=255) ggAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc < 1:734727/100‑1 (MQ=255) ggAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc < 2:734727/100‑1 (MQ=255) agagGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGTTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa > 2:313686/1‑100 (MQ=255) agagGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGTTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa > 1:313686/1‑100 (MQ=255) agagGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa > 1:440124/1‑100 (MQ=255) agagGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa > 2:440124/1‑100 (MQ=255) gggCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc > 1:357517/1‑100 (MQ=255) gggCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc > 2:357517/1‑100 (MQ=255) gggCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc > 1:709498/1‑100 (MQ=255) gggCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc > 2:709498/1‑100 (MQ=255) gCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg > 2:1420041/1‑100 (MQ=255) | AGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCC‑GGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAG > NC_000913/3781058‑3781242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |