| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,781,152 | 0 | A | G | 35.3% | 23.5 / 18.7 | 35 | intergenic (+135/‑63) | lldD/trmL | L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (19/3); new base G (0/12); total (19/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.30e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
GGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAG > NC_000913/3781053‑3781242 | gggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGa > 2:628097/1‑100 (MQ=255)gggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGa > 1:772573/1‑100 (MQ=255) ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAg > 1:1054429/1‑100 (MQ=255) agagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAggg > 2:1276903/1‑100 (MQ=255) gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAg > 1:324739/1‑100 (MQ=255) ccGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAg > 1:56353/1‑98 (MQ=255) gcgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAgcgcgc > 2:1170300/1‑91 (MQ=255) gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAGGCCGCACTGCTCACCAAt > 1:36169/1‑100 (MQ=255) aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCTCTCGCCCCTCC‑GGGGAGAGGGTCGGGGGGAGGGGA‑AAAGGCCGCACTGCTCACCAAttt > 2:876875/1‑100 (MQ=255) cTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAGGCCGCACTGCTCACCAAtttt > 1:991129/1‑100 (MQ=255) ccTCCCCCTGAAACGACCCCGCCCTTTTTTTTCCTCCGGCGCCCCCCG‑GGGGGGGGGGCGGGGGTGAGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTg < 2:140304/100‑1 (MQ=255) ctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAGGCCGCACTGCTAACCAATTTTCTGCTATCCt > 1:217398/1‑100 (MQ=255) tGAAACGACACCGCACTCTTTTTTTCCCCCTCGCCCCTCC‑GGGGGGGGGGCCGGGGTGGGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt < 1:131666/99‑1 (MQ=39) cacCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCa > 1:356135/1‑100 (MQ=255) ccGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt > 1:1071380/1‑100 (MQ=255) ccGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt > 2:327081/1‑100 (MQ=255) ccccttttttttCCCCCCCCCCCCCCCGGGGGAGGGGGCGGGGGGGGGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTaa < 1:661941/95‑1 (MQ=255) cttctgtccccccTCGCCCGCCG‑GGGGAGAGGGCCGGGGGGGGGGAA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg < 2:1058830/90‑1 (MQ=255) tttttttCCCCCCCGCCCCGCG‑GGGGGGA‑GGCCGGCGGGGGGGGATAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg < 2:878621/98‑1 (MQ=255) ttttctccccccTCGCCCCGCG‑GGGGGGGGGCCCGGGGTGGGGGAA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg < 1:319480/87‑1 (MQ=255) tttttCTTTCTCTCCCCTCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAg > 2:535040/1‑100 (MQ=255) ttttcCCCCCCCCCCCCCCG‑GGGGGGGGGGCCGGGGGGGGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAg < 1:329310/100‑1 (MQ=255) ttttCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAga > 2:590272/1‑99 (MQ=255) ttttCCCCCCCTCCCTCCG‑GGGGGGAGGGGGGGGGGGCGGGCA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc < 1:590276/97‑1 (MQ=255) ctccctccCCCCCCG‑GGGGGGGGGGCCGGGGTGAGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATg < 2:772568/99‑1 (MQ=255) gcccTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt > 1:720620/2‑100 (MQ=255) cccTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa > 2:316459/1‑100 (MQ=255) cccTCCCCCCCCG‑GGGGGGAGGGCGGGGGTGGGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa < 2:94804/100‑1 (MQ=255) cccccccccTCG‑GGGGAGGGGCCGGGGGTGGGGGAA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa < 1:215031/95‑1 (MQ=255) ccccTCG‑GGGGGGGGGGCGGGGGGGGGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACa < 1:1076698/98‑1 (MQ=255) ccccgggggAGGGGGCCGGGGGGAGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt < 1:1170311/95‑1 (MQ=255) tCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc > 1:75142/1‑100 (MQ=255) gggAGAGGGCCGGGGTGAGGGAA‑AAAGGCCGCACTGCGCACCAATTGTCTGCTATCCTGCCACCGCACTAAGGGGGCAGTATGCTAAACATCGTAGTTTa > 2:1147101/1‑100 (MQ=255) gaggGGGCGGGGGGGGGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg < 2:991120/100‑1 (MQ=255) gggCGGGGGGGGGGGAA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc < 1:862393/100‑1 (MQ=255) ggccgggggTGGGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg < 2:937911/91‑1 (MQ=255) gCCGGGGTGAGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg > 1:532/1‑100 (MQ=255) | GGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCC‑GGGGAGAGGGCCGGGGTGAGGGGA‑AAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAG > NC_000913/3781053‑3781242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |