Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	936,278	1	.	T	24.1%	142.1 / 68.7	58	intergenic (‑12/+22)	ECDH1_RS04620/ECDH1_RS04625	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base . (39/5);  new base T (11/3);  total (50/8)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-02

TGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTT  >  DH1/936190‑936427                                                                                           |                                                                                                                                                       tGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACT‑GC‑TTT‑Ac                                                                                                                                                  <  1:209999/94‑1 (MQ=31) tGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACT‑GC‑TTT‑Ac                                                                                                                                                  >  2:210159/1‑94 (MQ=31)   gTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCCGGAGT‑GC‑GTG‑AATTCTGCACTATATAATTGGTTGCGGGGGGAGTTTTTGACCCGCCGCCATTCGGGttctt                                                                                       >  5:619944/1‑148 (MQ=2)                   ttGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGTAATCGA‑TGT‑A‑TTCTTCGATGTATTTTTCATTGCGCGGGAGGGATTAGCACCGACGCCCTTAGGGTTATTAGTCCGACGAGCTaa                                                                        >  8:1205133/1‑149 (MQ=2)                               ccTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACT‑GC‑TTT‑ACTTCAGCTATGTTTAATTGGTTGCGGCGGCCGGTTTTGAACCGACGCCCTTCGGGTTATGATCCCGCCGAGAGACCAGGCTGCTcccc                                                           >  7:81622/1‑149 (MQ=11)                                                 gacgaGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAGT‑CC‑GTG‑ACTTCTGCTATGTTTATTTGGTTGCGCGGGGGGGATTTGACCCGCCCCGCTTGGGGTTATGATCCCGACGAGCTACAAGGCTGCTCCCCCGCGCGTcgggcgatg                                           >  1:111190/1‑141 (MQ=2)                                                        tACCAGGCTGCTCCACCCCGCGTCACCGTAGT‑CC‑ATA‑AATTCATCAAATTTTAATTGGTTGCGGCGTACTGATATGAACCGACGACCTTCGGCTTATGATCCCGCAGAGCAACAAGGCTGCTCCAAAAATAGTCAGTGGATGCTCACtat                                   >  4:1355039/1‑150 (MQ=9)                                                          ccAGGCTGCTCCACCCCGCGTCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACt                                >  3:432076/1‑151 (MQ=255)                                                              gctgctCCACCCCGCGTCACCGTAAT‑GC‑GTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCAGGATTTGACCCGACGACATTCGGGTTATGAGCCCGAAGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGATTCGCACTATACTCCGc                            >  4:281375/1‑151 (MQ=17)                                                                tgctCCACCCCGCGTCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACCAGCTACCAGCCTGCTCCACACCGAGTCCGTGGATGCGCACTATACTCCGCTc                          >  4:979352/1‑151 (MQ=255)                                                                 gctCCACCCCGCGTCACCGTAGT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCAGGATTTGAACCGACCACCTTCGGGTTATTATACCGACGAGCTACCAGGCTGCTCCCCACGTAGTCGGTTGATGAGCACTATACTCCGCt                           >  4:1162486/1‑149 (MQ=17)                                                                  ctCCACCCCGCGTCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgc                        >  5:892979/1‑151 (MQ=255)                                                                   tCCACCCCGCGTCACCGTACT‑GCTTTT‑ACTTCATCAAA‑TTTGATTGGTTGCGGGGGACGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACAAGGCTGATCCACCCCGCGTCAGCGGATGCGCACAATTCTGCCCTcg                         >  1:875796/1‑149 (MQ=11)                                                                   tCCACCCCGCGTCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGCCCTTCGGGTTATGAGACCGACGCGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgcg                       >  2:1285667/1‑151 (MQ=255)                                                                    ccACCCCGCGTCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGt                      >  2:416946/1‑151 (MQ=255)                                                                     cACCCCGCGTCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGAAGAGCTACCAGGCTGCTCCACACCGCGTCAGTGGATGCGCACTATACTCCGCTCGCGtt                     >  1:613916/1‑151 (MQ=255)                                                                       ccccctctCACCGTAGT‑GC‑TTT‑ACCTCCTGAAAATTTTATTGGTTGCGGGGGCAGGAGTTGAACCGACGACCTTCGGGTTATGAGCACGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTTGATGCTCACTATACTCCGCTGGCGtt                     <  3:1341430/142‑1 (MQ=17)                                                                       ccccGCGTCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACAGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtttt                   >  6:389940/1‑151 (MQ=255)                                                                        cccGCGTCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                  >  6:1289240/1‑151 (MQ=255)                                                                          cgcgTCCCCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGACCCGCCGCCCTCCGGGTTAGGAGCCCGCCGCGCTCCCAGGCGGAGCCCCCCCGCGGCCGGGGATGCGCCCTATCCTCCGCTCGCGtttgc                  >  1:517811/1‑147 (MQ=18)                                                                          cgcgTCACCGTACT‑GCTTTT‑ACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAATCGCAGTATACTACTCACGCGTTTTCAt                >  1:17268/1‑151 (MQ=1)                                                                          cgcgTCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGCCGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                >  2:1249923/1‑151 (MQ=255)                                                                          cgcgTCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                >  7:1257989/1‑151 (MQ=255)                                                                           gcttCACGGTACT‑GCTTTT‑ACTTCAT‑AAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg               <  2:17201/148‑1 (MQ=14)                                                                           gcgTCACCGTACT‑GCTTTT‑ACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATTAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACAGGAATCCCAGTATACTccaaaagatatttaatt               >  6:567751/1‑136 (MQ=14)                                                                           gcgTCACCGTACT‑GCTTTT‑ACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCCCt                           >  1:49065/1‑139 (MQ=11)                                                                           gcgGCACCGTACT‑GC‑TTTAAATGCATCAAATTTT‑ATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCt                           <  2:48900/139‑1 (MQ=14)                                                                             gTCACCGTACT‑GCTTTT‑ACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGAGGCGCACAATTCTACCCTAGAGTTTTCAttc              >  6:363678/1‑148 (MQ=11)                                                                             gTCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc              >  4:136888/1‑150 (MQ=255)                                                                              tCACCGTACT‑GCTTTT‑ACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACtat                                   >  3:560528/1‑128 (MQ=11)                                                                              tCACCGTACT‑GCTTTT‑ACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAAGCGGACTATACAACGCACGAGTTttcattca             >  4:1211902/1‑150 (MQ=11)                                                                              tCACCGTACT‑GCTTTT‑ACTTCAT‑AAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACtat                                   <  4:560530/128‑1 (MQ=11)                                                                              tCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa             >  1:688640/1‑150 (MQ=255)                                                                              tCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa             >  8:363850/1‑150 (MQ=255)                                                                              tCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa             >  5:989183/1‑150 (MQ=255)                                                                               cACCGTACT‑GCTTTT‑ACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGAAGCGAAATATACAACGATCGATATTTCATTCAAc           >  6:1203914/1‑151 (MQ=11)                                                                               cACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc           >  8:890561/1‑151 (MQ=255)                                                                                 ccGTACT‑GCTTTT‑ACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGCGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGCGTACTATACACCGCTGGCGTTTTccttgcggcg         >  8:1080697/1‑142 (MQ=11)                                                                                 ccGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt         >  2:1360667/1‑151 (MQ=255)                                                                                 ccGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt         >  5:1355973/1‑151 (MQ=255)                                                                                 ccGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt         >  4:587960/1‑151 (MQ=255)                                                                                  cGTACT‑GCTTTT‑ACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATTCGGACTATACTCCGATAGATTTTTCAt                >  7:1236732/1‑143 (MQ=1)                                                                                  cGTACT‑GCTTTT‑ACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCGCTCGCGTTTTCATggcgccgt        >  5:433251/1‑144 (MQ=14)                                                                                  cGTACT‑GCTTTT‑ACTTAAT‑AAATTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                <  8:1236059/143‑1 (MQ=17)                                                                                  cGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt        >  6:915438/1‑151 (MQ=255)                                                                                  cGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt        >  7:630878/1‑151 (MQ=255)                                                                                   gTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt       >  5:1344208/1‑151 (MQ=255)                                                                                    tACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt      >  6:742809/1‑151 (MQ=255)                                                                                    tACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt      >  1:1070271/1‑151 (MQ=255)                                                                                    tACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGCATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt      >  5:1111601/1‑151 (MQ=255)                                                                                     aCT‑GC‑TTT‑TAATAATAAAATTTTTATTGGTTGCGGTGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt     <  5:568273/151‑1 (MQ=21)                                                                                      cT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt    >  6:1351297/1‑151 (MQ=255)                                                                                      cT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt    >  8:403161/1‑151 (MQ=255)                                                                                       t‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTAATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCCTCCGTGGATGCGCACTATACTCCGCTCGCCTTTTCATGCAACCtttttgt   >  4:601768/1‑149 (MQ=255)                                                                                       t‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGAACTTCGGGTTATGAGCCCGACCAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGGTTTCATGCAACCttttttt   >  8:662269/1‑151 (MQ=255)                                                                                         gC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt  >  8:1329139/1‑151 (MQ=255)                                                                                         gC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt  >  3:45453/1‑151 (MQ=255)                                                                                         gC‑TTT‑ACCTAATTAAATTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACGTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt   <  3:155854/150‑1 (MQ=255)                                                                                          c‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt  >  7:496599/1‑150 (MQ=255)                                                                                           |                                                                                                                                                       TGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACT‑GC‑TTT‑ACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTT  >  DH1/936190‑936427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑