BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	1,647,669	0	A	C	22.5%	308.7 / 31.2	233	intergenic (+248/+125)	dusC/ECDH1_RS08050	tRNA dihydrouridine synthase DusC/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (141/28); new base C (49/0); total (201/32)
Fisher's exact test for biased strand distribution p-value = 9.98e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

GCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTAAATACGCC  >  DH1/1647519‑1647817

      gCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGg‑cc                                                                                                                                                 <  4:374600/151‑1 (MQ=31)

       cAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCACGGGGCGGCGGCG‑ATTGg‑ccc                                                                                                                                                >  8:229587/1‑150 (MQ=14)

        aGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑CTTGg‑ccgc                                                                                                                                               >  1:1951919/1‑151 (MQ=14)

          cccACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGccc                                                                                                                                             <  4:1212595/151‑1 (MQ=31)

          cccACATGGATGGGGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGTATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGccc                                                                                                                                             <  4:2022925/151‑1 (MQ=14)

            cacaTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGccccc                                                                                                                                           >  3:11910/1‑151 (MQ=31)

            cacaTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGccccc                                                                                                                                           <  6:1785336/151‑1 (MQ=31)

             ccagggtggtgGGCGGAGGGGCCTTTTTCCCGGGTTGTTACCTGCCCGCCC‑ACCGGTTAGCGGTAAGGTAAACGCGGTGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCt                                                                                                                                          <  7:742191/143‑1 (MQ=2)

             acaTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCt                                                                                                                                          >  1:808744/1‑151 (MQ=31)

             acaTGGATGGGGGCTGCGGGCGGGTTTTACAGGGATGTACCCT‑CGCCCCCGACCGGGTAGCCGTACGGTATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCt                                                                                                                                          <  7:212951/151‑1 (MQ=11)

              caTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTg                                                                                                                                         >  5:7863/1‑151 (MQ=31)

              caTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTg                                                                                                                                         <  7:1323036/151‑1 (MQ=31)

              caTGGAGGGGGGCGGCGGGCGCGTTTTACCGGGAGGTTACCT‑CGCGCCCGACCCGGTAGCCGTACGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTg                                                                                                                                         <  3:1948618/151‑1 (MQ=11)

               aTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTgc                                                                                                                                        <  1:127193/151‑1 (MQ=31)

               aTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTgc                                                                                                                                        <  1:1467653/151‑1 (MQ=31)

                  gttgggggCGGAGGGCGCGTTTTACAGGGATGTTCCCG‑CGCGCCCTACCCGGTAGCCGTAAGGTATACGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTgcgcg                                                                                                                                     <  3:912473/146‑1 (MQ=11)

                  gATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑GCGCCCCCTgcgcg                                                                                                                                     >  6:535851/1‑151 (MQ=14)

                  gATGTGGGCTGAGGGCGCGTTTTACAGGGAGGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTgcgcg                                                                                                                                     <  2:907264/151‑1 (MQ=14)

                    tgtgGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCt                                                                                                                                   >  7:1178770/1‑151 (MQ=31)

                       gggCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGGCCGGGGCGGCGGCG‑CTTGT‑GCCCCCCCCgcgcccggcc                                                                                                                                >  5:450668/1‑145 (MQ=11)

                       gggCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCt                                                                                                                                >  8:1048117/1‑151 (MQ=31)

                           tGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCCCGCTCCTTgcg                                                                                                                            >  1:1501381/1‑151 (MQ=14)

                           tGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑AGTGG‑CCGCCCCCTGCGCGCGCCTTgcg                                                                                                                            >  1:532569/1‑151 (MQ=14)

                             aGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCACGGGGCGGCGGGG‑CTTGG‑CCGCCCCCTGCGCGCGCCTTGCGcc                                                                                                                          >  3:625438/1‑151 (MQ=9)

                              gggCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGcc                                                                                                                          >  4:1083988/1‑150 (MQ=31)

                               ggCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCACCTTGCGCCac                                                                                                                        >  2:1114679/1‑150 (MQ=14)

                                gcgcGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑GCGCCCCCTGCGCGCTCCTTGCGCCacg                                                                                                                       >  1:1322795/1‑149 (MQ=14)

                                  gcgTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTg                                                                                                                      >  2:1184762/1‑150 (MQ=31)

                                   cgTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGAt                                                                                                                    >  2:1806321/1‑151 (MQ=33)

                                   cgTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGAt                                                                                                                    >  2:1503615/1‑151 (MQ=33)

                                    gTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑CTTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCCGTGAtt                                                                                                                   >  8:1422304/1‑151 (MQ=18)

                                    gTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑CTTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGGGAtt                                                                                                                   >  5:1563852/1‑151 (MQ=18)

                                     ttttACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGAttt                                                                                                                  >  7:600059/1‑150 (MQ=18)

                                       ttACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGGGATtat                                                                                                                 >  1:1074674/1‑150 (MQ=21)

                                        tACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑GTTGG‑CCGCCCCCGGCGCGCGCCGTgcggccgggcgtatat                                                                                                               >  7:351941/1‑138 (MQ=11)

                                           aGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGAGTATATGa                                                                                                             >  6:2042747/1‑150 (MQ=25)

                                              gATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑CTTGG‑CCGCCCCCGGCGCGCTCCTTGCGCCAGTGCTTATATGAAG‑c                                                                                                         >  1:831796/1‑150 (MQ=255)

                                                  ttACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCACGGGGCGGCGGCG‑CTTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAC‑CAATGc                                                                                                    >  3:313812/1‑151 (MQ=255)

                                                   tACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑CTTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGcc                                                                                                   >  4:11665/1‑150 (MQ=255)

                                                   tACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCACGGGGCGGCGGCGCATTGG‑‑CGCCCCCAGCGCCCGCCATGCGCCACTGATTTTTAGAAG‑CAATgg                                                                                                    >  8:726867/1‑149 (MQ=11)

                                                       t‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATg                                                                                               >  3:1517051/1‑151 (MQ=255)

                                                       t‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAC‑CAATGCCCATg                                                                                               >  2:564210/1‑151 (MQ=255)

                                                           tgcCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTTTATGCAG‑CAATGCACATGAc                                                                                             >  4:1603311/2‑150 (MQ=255)

                                                           cgcCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCCGTGATTATATGAAG‑CAATGCCCAGGAc                                                                                             >  3:166068/1‑150 (MQ=255)

                                                            gcCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTTCGCCAGTGATTATATGAAG‑CAATGCCCATGaa                                                                                             >  2:1773763/1‑148 (MQ=255)

                                                            ccccgccggggTAGCCGTAAGTGATACGGGGGGGGCCCCGGGGCGCGGGTATTTTGTGCGCCAGCGCGGGGGGGGGCGGGGGGCGGGGGCT‑ATTGG‑CCGCCCCCGGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAg                                                                                           <  3:1072305/142‑1 (MQ=255)

                                                             cccGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑CTTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAgg                                                                                          >  5:1058838/1‑151 (MQ=255)

                                                              ccGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAgg                                                                                          >  8:1666730/1‑150 (MQ=255)

                                                                   ccGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCCGCG‑ATTGG‑CCGCCCCCTGCGCGCACCTAGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAgcgc                                                                                     >  8:179803/1‑150 (MQ=255)

                                                                    cGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCa                                                                                    >  2:867131/1‑150 (MQ=255)

                                                                     ggTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCACGGGGCGGCGGCG‑ATTTG‑CCGCCCCCTGCGCGCTGCTTTCGCCAGTGATTATATGAAG‑CAATGGACATGACAGGAGCGCaa                                                                                   >  6:765036/1‑150 (MQ=255)

                                                                      gTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCAGCCCGCTCCATGCGCCACTGATTATATGAAG‑CAATGCACATGACCGGAGCGCAAc                                                                                  >  4:660735/1‑150 (MQ=255)

                                                                        aGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑CTTGG‑CCGCCCCCGGCGCGCTCCTTGGGCCAGTGATTATATGAAG‑CAATGCCCATGACAGGCGGGCAACAgg                                                                               >  3:1908960/1‑151 (MQ=17)

                                                                        aGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAgg                                                                               >  2:251765/1‑151 (MQ=255)

                                                                        aGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAgg                                                                               >  3:1496145/1‑151 (MQ=255)

                                                                         gCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAgg                                                                               >  8:2025331/1‑150 (MQ=255)

                                                                           cGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATg                                                                            >  1:379448/1‑151 (MQ=255)

                                                                             tAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATTCACATGACAGGAGCGCAACAGGATg                                                                            >  4:1413950/1‑149 (MQ=255)

                                                                             tAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCC‑ATTGG‑CCGCCCCCTGCGCGCGCCTTGCGCCAGTGATTATATGAAG‑GCATGGACATGACAGGCGCGCAACAGGATg                                                                            >  6:701720/1‑149 (MQ=17)

                                                                              aaGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑GCGGCCCCTGCGCGCGGCTTGGGGCCGGGGTTTTATGAAG‑CAATGCAGATGACAGGGGCGCAACAGGGTgggc                                                                         >  6:1543706/1‑148 (MQ=17)

                                                                              aaGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATg                                                                            >  4:969985/1‑148 (MQ=255)

                                                                                  gATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGCGGCGGCG‑CTTGG‑GCGCCCCCCGCGCCCGCCGTGTGGCAGGGCTTTTATGGAG‑CCATTCACAAGACAGGGGCGCcacacgcttcgagccg                                                                     >  7:991815/1‑139 (MQ=9)

                                                                                     aaGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGCGGCCGCG‑CTTGG‑CCGCCCCCAGCGCGCGCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCCACAGGATGAGAGAcggc                                                                   >  8:1521901/1‑147 (MQ=18)

                                                                                      aGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAC‑CAATGCACATGACAGCAGCGCAACACGATGACCGACTGaa                                                                  >  3:788496/1‑150 (MQ=255)

                                                                                       gCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCACGGGGGTGCAGGGGGCGGCGGCG‑AGTGG‑CCGCCCCCTGCGCGCGCCGTGCGCCAGTGCTTATATGAAG‑CCCTGCCCCTGGCAGGAGCGGACCAGGATGAGAGACTGTAat                                                                >  7:1682643/1‑151 (MQ=17)

                                                                                          gAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCACGGGGCGGCGGGG‑TTTGG‑CCCCCCCCCGCCCGCGCCTTGGCCCCGTGGATTTATGAAGCCAATGC‑CCTGGAAGGAGCGCACCAGGGAGTGAGCCTGCAattat                                                             >  1:2020445/1‑148 (MQ=2)

                                                                                           aGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑CTTGG‑CCGCCCCCGGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CCATGCCCCAGACAGGCGCGCCACACGATGAGAGGCTGAAATAttt                                                            >  7:702162/1‑150 (MQ=17)

                                                                                           aGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATAttt                                                            >  5:1700826/1‑150 (MQ=255)

                                                                                                  cgcgCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑GCGCCCCCAGCGCGCGCCGTGCGCCAGTGATTATATGAAG‑GAAAGCACATGACAGGAGCGCAACAGGATGAGAGACGGGAATATTATCGtttt                                                     >  2:951597/1‑149 (MQ=18)

                                                                                                    cgcAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑CTTGG‑CCGCCCCCTGCCCGCGCCGTGCGCCAGTGCTTTTATTAAG‑GAATGCCCATGACAGGAGCGCCACAGGCTGAGCGACTGAAATATTGTGGTTAttt                                                   >  5:2002868/1‑151 (MQ=18)

                                                                                                      cAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGCTGAGAGACTGAAATATTCTCGTTATTTTc                                                 >  3:40441/1‑151 (MQ=255)

                                                                                                       aGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGAACCGGGCCGCCGCC‑AATGG‑CCGCCCCCAGCCCGCTCCTTGCGCCAGAGATTATATGAAC‑GAATGCACAAGACACGACCGCAACAAGATGAGAGACAGAAATAATCTCGTTAttttgg                                                >  4:1568129/1‑149 (MQ=18)

                                                                                                          ggcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑GTTGG‑CCGCCCCCGGCGCGCGCCGTGCGGCCGTGGTTATATTTAG‑CAAAGCACATGACAGGCGCGCAACAGGGTGGGAGCCGGAAATATTCTCGGTTTTTTgggcc                                             >  1:321074/1‑149 (MQ=17)

                                                                                                          ggcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGAc                                             >  4:59939/1‑151 (MQ=255)

                                                                                                          ggcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATCCACAAGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGAc                                             >  2:155210/1‑151 (MQ=255)

                                                                                                          ggcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCCGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGACACAGAAATATTCTAGTTAttttacgac                                             >  6:1982050/1‑146 (MQ=21)

                                                                                                           gcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑CTTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGCGCGCAACAGGATGAGAGCCTGAAATATTCTCGTTATTTTCGGAcc                                            >  1:877427/1‑151 (MQ=255)

                                                                                                            cgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCt                                           >  8:1892908/1‑151 (MQ=255)

                                                                                                               ttttGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCACGTTATTTTCGGACCtgat                                        >  4:1843701/1‑151 (MQ=255)

                                                                                                                  tGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAAAATTCTCGTTATTTTCGGACCTGATGAc                                     >  4:1362604/1‑151 (MQ=255)

                                                                                                                   gTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGAc                                     >  6:817344/1‑150 (MQ=255)

                                                                                                                        ccAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGCCAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCGGAAGACaataa                                >  6:997248/1‑150 (MQ=255)

                                                                                                                         cAGATCCCGGGGGTGCAGTGGGCGGCGGCG‑ATTGG‑CCGCCCACGGAGCCCTCCGTGGGCCAGGGATTATTTGCAT‑CAATGCACATGCCAGGCGCGCAACAGGATGAGAGACAGAAATATTCTCGGAATTTTCGGACCGGATGCCattaatt                              >  8:610990/1‑151 (MQ=21)

                                                                                                                         cAGAGCCCGGGGGTGCAGGGGGCGGCCGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCACTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAAAATTATCGTTATTTTAGGACCTGATGACattaatt                              >  6:712730/1‑151 (MQ=255)

                                                                                                                           cgcgcggggggggggggggggggggggG‑TTTGGCCCCCCCCCCCCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACattaatta                             <  2:144036/136‑1 (MQ=255)

                                                                                                                                cGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACTGGATGAGAGACTGAAATATTCTCGTTATTTTCGGAGCTGATGACATTAATTAAtttt                        >  6:269932/1‑150 (MQ=255)

                                                                                                                                cGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAAtttt                        >  4:1640453/1‑150 (MQ=255)

                                                                                                                                cGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAAtttt                        >  4:1919994/1‑150 (MQ=255)

                                                                                                                                cGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAAtttt                        >  8:1342049/1‑150 (MQ=255)

                                                                                                                                 gggggTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTa                       >  2:525722/1‑150 (MQ=255)

                                                                                                                                                 gcggcg‑TTGGG‑CCGCCCCCGGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTAAATa      <  4:1101338/151‑1 (MQ=255)

                                                                                                                                                 gcggcg‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGCGCGCAACAGGATCAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATCACATTAATTAATTTTAAAATATCTTATTAAAt       >  6:1598890/1‑150 (MQ=255)

                                                                                                                                                 gcggcg‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTAAAt       >  5:685389/1‑150 (MQ=255)

                                                                                                                                                  cggcg‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTAAATAc     >  2:502123/1‑151 (MQ=255)

                                                                                                                                                   ggcg‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTAAATACg    >  1:1830842/1‑151 (MQ=255)

                                                                                                                                                    gcg‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTAAATACGc   >  3:1921247/1‑151 (MQ=255)

                                                                                                                                                     cg‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATATTAAATACGcc  >  8:22264/1‑151 (MQ=255)

                                                                                                                                                      g‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTAAATACGcc  >  3:1275651/1‑150 (MQ=255)

                                                                                                                                                      g‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTAAATACGcc  >  1:1644359/1‑150 (MQ=255)

                                                                                                                                                      g‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATAATCTTAAATACGcc  >  8:1910707/1‑150 (MQ=255)

GCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCT‑CGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCG‑ATTGG‑CCGCCCCCTGCGCGCTCCTTGCGCCAGTGATTATATGAAG‑CAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTAAATACGCC  >  DH1/1647519‑1647817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`