BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	3,906,837	0	C	G	22.8%	197.6 / 15.2	112	intergenic (+8/‑27)	ECDH1_RS19315/ECDH1_RS19320	tRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base C (61/17); new base G (23/0); total (93/19)
Fisher's exact test for biased strand distribution p-value = 1.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

GGGGGTTCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATT  >  DH1/3906687‑3906986

ggcggTTCAAGTCCCCCCCCTCGCACCAAAAACCACGTAGATATTGCTCGCACTGCGCGAAGGTGGCGCAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGc                                                                                                                                                        <  4:333983/148‑1 (MQ=255)

 ggcggcaaaGGCCCCCCCCTCGCACCAACAACCTCTTTTAAATTGGTGCCCGTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTGCTTACGGACGTG‑GGGGTTCAAGGCCCCCCCCTC‑GCACCAAACGAGGCg                                                                                                                                                       <  5:353238/144‑1 (MQ=14)

      ccaacgcccccgccTCGCACCAACAACGACGTTTATATTGCTCGCGCTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATc                                                                                                                                                  <  1:354411/145‑1 (MQ=18)

        aactCCCCCGCCTCGCAGCAAAAAACTCGTTGATATTCCTCTCCCTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATCaa                                                                                                                                                <  8:37830/148‑1 (MQ=18)

           gccccccccTCGCACCAAAAACAACGTTGATAGAGGTGGCCGTATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATCaaaaa                                                                                                                                             <  7:167421/150‑1 (MQ=17)

             cccccccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTCAGAACCAAA‑GAGCCGAGATGaaagaa                                                                                                                                            >  6:806973/1‑150 (MQ=18)

                ccccTCGGTCGAAAAAACACGTTTTTATTGCTCCCAGTTGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATCAAAAAAAGTa                                                                                                                                        <  1:1042/151‑1 (MQ=18)

                 cccTCGCTCCAAAAACGACGTTGATATTTCTCTGCGTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATCAAAAAAAGTaa                                                                                                                                       <  3:1019915/151‑1 (MQ=18)

                    tCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAATAAGTGTAAATCAAAAAAAGaaacac                                                                                                                                    >  1:485365/1‑148 (MQ=18)

                      gCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAAAACTGTAAATCAAAAAAAGTAACATg                                                                                                                                   >  4:922668/1‑150 (MQ=18)

                        aCCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTAAAGTCCCCCCCCCC‑CCACCAAAAAAGGGTAAATCAAAAAAAGtttatttcat                                                                                                                                >  7:86528/1‑142 (MQ=18)

                         ccAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCAA‑GCACCAAAAAACGATAATTCAAAAAATGTAAAGTGACtg                                                                                                                               >  4:1959116/1‑151 (MQ=17)

                         ccAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTACACGTGTTAGTGTTCTTAGTCACGGC‑GTGGTTAGAGGGCCCCCCCTG‑GCCAAAAACGAAGAAATAAAAAAAAAGGTAAGATggggg                                                                                                                               >  4:1762242/1‑147 (MQ=16)

                          caacaaCCACGTTTATATTGCTGTCAGTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtgt                                                                                                                              <  7:1156545/151‑1 (MQ=255)

                          cAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACTAGTGGATTTCaataattgttagttccctgt                                                                                                                              >  3:1284986/1‑136 (MQ=17)

                             aaaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAAAAGTAGAGATCAAATAATGtttcatgcatgattt                                                                                                                           >  8:664232/1‑137 (MQ=17)

                             aaaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTAAAGTCCCCCCCCTA‑GCACCAAACAAGTTTATATCAATAAAAGTTAAATGAgtgttat                                                                                                                           >  7:1296669/1‑148 (MQ=18)

                                   cGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTg                                                                                                                     <  7:109330/151‑1 (MQ=255)

                                   cGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAATTTAGGTATATCAAAAATATTTTGATGCCtgtgtttagttg                                                                                                                     >  6:1413955/1‑143 (MQ=14)

                                      ttatatTGGTCGCCCTGCGAGAAGGTTGCGGATTTGGTAGAGGCGCTAGCTTCAGGGGTTAGTGTGCTTACTGAGGTG‑GGGGGTCAATTCCCCCCCCTC‑GCACCAAACGAGTAGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTgga                                                                                                                   <  7:1644075/148‑2 (MQ=255)

                                      tGATATTGGTCGCACTGGGGGAAGGTTGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGc                                                                                                                   <  7:1644068/150‑1 (MQ=255)

                                      tGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCAC‑GCACCAAAAAAGGATAAATAAAAAAAAGTAAaatgaatgatatttaaaagcg                                                                                                                  >  6:455251/1‑138 (MQ=14)

                                         tatTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAGGAGGGTAGTTCAAATAAAGTTTGTTGCCTGTGGTTTGGTGGCGGat                                                                                                               >  5:1690232/1‑150 (MQ=14)

                                               ccGCACAGGGCGCAGGCGGCGGAATTGGTTTTGCCGCGAGCTTCAGGCCTTAGTGATCTTCCGCGCTGC‑GGGGTGGTATGCCCCGTGCTC‑TCCCGAACCGACGCGATATCCATAAAAGTCAGATGACTGTGCGAAGGTGGCGGAATTGGTa                                                                                                         <  3:1763345/150‑1 (MQ=255)

                                                gggttttgtagAAGTTGGCGGAATAGGTAGACGCGCTAGGTTCCGGTGTTTGTGTGGTTTCCGA‑GTGCGGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTa                                                                                                         <  5:12466/140‑1 (MQ=21)

                                                 ttttttttttaaGTTGGCGGAATTAGTATTAGCTCTAGCTTCAGGTTTTAGTTTTCTTACGTAGCTG‑GTGTTGCTAGTCACCAGCCTC‑GCACTAAAGGCGTCGATATCATTAAAAGTAATCTTACTTTGCGAAGGTGGCGGAATTGGTAg                                                                                                        <  6:510648/140‑1 (MQ=255)

                                                   aCTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAAAAGGAGAAATAAAAAAAAGTATGATGAATGTTATTTGGTGGCTGAATAGGTataat                                                                                                     >  4:663306/1‑147 (MQ=14)

                                                   aCTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑CCACCAAAAGTGGCTAAATCAAAAAAAGTAACATCCCTGTTGTAACGTGGCAGAAtaacaaga                                                                                                       >  5:1664833/1‑142 (MQ=14)

                                                      gggCGAAGGTGGCGGTCTTGCTAGTCGGCGTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGCCCCCCCCCTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGAcgcg                                                                                                   <  7:682962/150‑1 (MQ=25)

                                                          gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAATTGGATAAATCAATAAAAGTTACATCATTGTTTGTTGGTGGCTGAAttattataatttatat                                                                                               >  5:990094/1‑136 (MQ=2)

                                                                    gcagccgaacccgcgCTAGCTACAGGTGGTAGTGTTCTTCCGGACGTG‑GTGGTTCATGTCCACCGCCTC‑GCACGAAAAGAGGCTATATCAAATAAATTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGtt                                                                                    <  6:19018/140‑1 (MQ=25)

                                                                        tGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTATCCGCGGTAGCGTCAGGTGTTAgtg                                                                                >  3:1694783/1‑151 (MQ=255)

                                                                               cgcgCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAAGAGGCTAGATCAAAAAAAGTATGATGACTGTGGGATTGTGGCGGAATTTGTATAAGCGCTATCTTCAGGTGTTATTGTACtttc                                                                         >  3:308369/1‑149 (MQ=18)

                                                                                        ttCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAACAGGCGAGATCAAAAAAAGTTAGATGACTGTGGTAAGATGGCGGAATTGCTATACTTGAAAGCTGCAGGTGTTActgtaataaagcaagtggg                                                                >  5:1290727/1‑136 (MQ=16)

                                                                                         tCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAGGAGGGTATATCATAAATTGTTTGTGGCGTGTTGGTTGGTGGCGGAATTGATTGAAGGGGAAGCTTCAGGTGTGAGTGTAATTGGGGACgg‑gggg                                                              >  4:883517/1‑151 (MQ=9)

                                                                                          cAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTGCCCCCCCTC‑GCACCAAAAGAGGGGATATCAAAAAAAGTAAGTTGACTGTGATAAGGTGGGGGAATTTGTAGAAGGGGTAGCTTCAGGTTTTAGTGTACATAGGGACGT‑ggggg                                                             >  7:944478/1‑151 (MQ=21)

                                                                                             gtgtTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAAAAGGGGATTTCAAAAAAAGTAAGATGACTGTTGGATGGTGGCGGAATTGTTATAAGGGGTATCTGCAGGTGGTAGTGTAGTTAAGGCCGG‑GGGGCttg                                                          >  4:883761/1‑150 (MQ=16)

                                                                                             gtgtTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAAAACGAGATATCAAAAAAAGTAAGATGAATGTGATAAGGTGCCGGAATTGGTAGAATAGAGAGCTTCAGGTGTTAGAGTCCTTAAGGACGT‑GGGGGttg                                                          >  4:1347890/1‑150 (MQ=21)

                                                                                               gtTAGTGTTCTTACGGACGTG‑GGGGTTGAAGTCCCCCCCCGC‑GCCCCAAAAGAGGCGATATGAAAAAAAGTTAGATTACTGTTGGTATGTGGGGGAATTGGTATAAGAGGTAGCTTGATGTTTTAGTGTAATTTCGGCCGT‑gggggggggt                                                        >  7:62989/1‑146 (MQ=255)

                                                                                               gtTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACTAGGGGATTTCAAATAAAGTTAGATGAGTGTGGTTTGGTGGCGGAATTGGTATACTGTTTTGGTTGAGGTGTTACTGTCCTTACGGCCGT‑GGGGGGTCa                                                         >  1:782699/1‑150 (MQ=16)

                                                                                                ttAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAGGAGGGGATATCAAAAAAAGTTAGTTGAGTGTTGTTTGGTGGCGGAATTGGTATACTGGGTAGCTTCATGTTTTACTGTAATTAAGGCCGG‑GGGGGttga                                                         >  3:1470858/1‑147 (MQ=16)

                                                                                                 tacgtgtCTTTCGGACGTG‑TGGGCTCAAGTCCCCCCCCTC‑GCACCACACGAGCCTATATCAAATAAAGTAAGATCACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAg                                                       <  5:1397899/144‑1 (MQ=21)

                                                                                                  aGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCCCCAAACGAGGCGATATCAAAAAAAGTAAGATTACTGTGCGAAGGTGGCGGAATTTGAATACGCGGGAGCTTCAGGTGTTAGTGTCCTTACGGAAGT‑GGGGGTTGaacgc                                                     >  5:1616440/1‑148 (MQ=37)

                                                                                                    tgtTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAGGAGGGGATATCAAAAAAAGTTTGATGCGTGTGGGTAGGTGGCGGATTTGGTATAAGGGGGAGCTTCAGGTGGTAGTGTACTTACGGACGA‑GGGCGCTCCAGTgcc                                                   >  1:1603408/1‑151 (MQ=16)

                                                                                                    tgtTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGAGTTCAAAAAAAGTATGATGACTGTGGGAAGGTGGCGGATTTGGTTGAAGGGGTATCTTCATGTGTTAATGTAAATAAGGACGG‑GGGGGTTGAAgcaa                                                    >  3:486280/1‑147 (MQ=21)

                                                                                                     gtTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAAGAGGCGATATCAAAAAAAGTAAGATGACTGTGGGAAGGTGGCGGAATTGGTATACGCGATAGCTTCAGGTGTTATTGTACTTACGGACGT‑GGGGGTTCAAGGcccc                                                  >  1:1722431/1‑151 (MQ=37)

                                                                                                       tCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAGGAGGGTATTTCAATAAAAGTTAGTTGCATGTTGTTTGGTGGCGGAATTGGTATCAGGGGTATCATCATGTTTTAGTGTAATAAGGGCCGT‑GCGGGTTGAAggaaaacc                                                >  1:1603403/1‑144 (MQ=9)

                                                                                                       tCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAGGAGGGGAAATCAAAAAAAGTTTGATGAATGTGTGATCGTGGCGGAATTGTTATACTGGGTATCGTCATGTGTTATTGTCCTAACGGCCCT‑GGGGGtggtagttcccac                                                >  3:1391631/1‑139 (MQ=16)

                                                                                                       tCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAGGACGGGAAATCAATAAATGTTTGATGCATGATATAAGGTGGCGGAATTGGATTAAGAGCAAGCAGCAGGGTTTAGTGTAATAAAGCACGT‑CCGGGTTCAAggaacaac                                                >  2:528068/1‑144 (MQ=2)

                                                                                                        cTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAGGAGGCGAGTTCAAAAAAAGTAAGATGCCTGTGTTAAGGTGGCGGAATTGGTATCAGGGCGAGCTGCAGGTTTTATTGTCATAAGGGACGT‑GGGGGtggaaggcacaccc                                               >  1:1606274/1‑138 (MQ=16)

                                                                                                         ttACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAGGAGGGGATATCAAAAAAAGTTAGATGCCTGTGCGAAGGTGGCGGAATTGGGATACGCGCGAGCATCATGTGGTACTGTCCATACGGCCGT‑cgggggtgcagcccaacccc                                              >  1:1560556/1‑136 (MQ=21)

                                                                                                         ttACGGACGTG‑GGGGTTCAAGTCCCCCCCCGC‑GCCCCAAAACAGGGGATATCAAAAAAAGTTAGATGACTGTGGTATCGTGCCGGAATTGGTAGACGCCAGAGCAGCAGGTGTTACTCTACATAAGGCCGT‑GGCGGGTGAACTcccccccc                                              >  3:1592320/1‑151 (MQ=255)

                                                                                                          tACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCCCCAAAAGAGGGGAAATC‑AAAAAAGTAAGATGCCTGGGGGTATGTGGCGGGATTGGTAGACGCGCTAGCAACCGGGGTTAGTGTCCTTACGGACCG‑GGGGGGTGGAGGcccccccca                                             >  6:1622083/1‑149 (MQ=19)

                                                                                                          tACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAAAAAGCTAAATCAAAAAAAGTAAGATGAATGTTCTAAAGTGGCGGAATTACTATACGAAATATCATCATGTGTTAATGAAAATAAGGAAGT‑GCGGGTTCaactaaaaccaa                                              >  8:1106233/1‑140 (MQ=9)

                                                                                                          cacgccctcg‑ggcgTTCCAGTCCCCCCCCCC‑GCACCACACGCCGCTATAACAATAATATTTATATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCt                                             <  4:900925/142‑1 (MQ=14)

                                                                                                            cGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAGGAGGGGATATCAAAAAAAGTAAGTTGACTGTGGGATGGTGGCGGAATTGGAAGACTGGGTAGCTTCAGGTTTTAGTGTAGTTACGGACGT‑GGGGGTTGAAGGACACACCCTCg                                           >  1:1470148/1‑151 (MQ=25)

                                                                                                             ggACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAAGAGGCTATATCAAAAAAAGTTAGATGACTGTTGTAAGGTGGCGGAATTTGTATAAGCGAGATCATCAGGTTTTACTGTAATTAAGGACGT‑GGGGGGTGAAggaaacaccaaagc                                          >  7:1402534/1‑138 (MQ=16)

                                                                                                              gACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAGGAGTGGATATCAAAAAAAGTAAGATGAGTGTGAGATCGTGGCGGAATTGGTATACTAGGTAGCTTCAGGTTTGACTGTAGTTACGGACGT‑GGGGCTTGAAgcaacagccaatgcc                                         >  7:1931102/1‑137 (MQ=255)

                                                                                                              gACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATCAATAAAAGTAAGATGCCTGTTCGAAGGTGGCGGAATTGGTAGACGAGCTAGCTTCAGGTGTTAGTGTACTTACGGACGT‑GGGGGTGGAAGTCCCCCCCATCGc                                          >  3:1308388/1‑150 (MQ=25)

                                                                                                              gACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGAGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGGGGTAGGCCCCCCCCTCGcc                                         >  7:1480691/1‑150 (MQ=25)

                                                                                                                cGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAAAGAGGAGAGATCAAAAAAAGTAAGATGAATGTGGGAAGGTGGCGGAATGGGAAGACGGCGTAGCTTCAGGTGTAAGTCTCGTTGCGGCCGT‑GGGGGTGCAAGGcaccccccaggcccc                                       >  8:1679588/1‑144 (MQ=255)

                                                                                                                 gTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATCAAAAAATGTAAGATCCCTGAGGGAAGGTGGCGGAATTGGTAGCCGAGGGAGCTTCAGGTGTGAGTGTCCTTACGGCCGT‑GCGGGTTGAAGGCCCCCGCCTCGcccc                                       >  8:494866/1‑150 (MQ=21)

                                                                                                                  tG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGGTGGAGGccgccccccgcccgca                                      >  8:812474/1‑142 (MQ=25)

                                                                                                                           cAAGTCCCCCCCCTC‑GCACCAAAGGAGGCGATATCAAATAAAGTATGTTGAGTGTGGTATGGTGGCGGATTTCGTTTCAGCGGGAGCTTCAGGTTTTAGTGTCGTTCCGGCCGT‑GCGGGTTGACGTCCCCCCCCGCGccccctgaaatctt                             >  3:1139545/1‑141 (MQ=255)

                                                                                                                                 cccccccTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGGGGAAGTGGCCCCCCGGGCCCCAAGTATGTTTACtt                         >  8:883325/1‑149 (MQ=21)

                                                                                                                                  ccccccTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCCCGGCCCCCCCCCGCGCCCCCATTATCTTTACGTcg                       >  7:525193/1‑150 (MQ=21)

                                                                                                                                  ccccccTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCGCGCCCCCATTATTGTTACTTCCt                      >  3:745523/1‑151 (MQ=255)

                                                                                                                                  ccccccTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCGCGCCCCAATTATCTTTAGTTCCt                      >  6:1584138/1‑151 (MQ=255)

                                                                                                                                   cccccTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑CGGGGTTCAACTCCCCCCCCTCGCACCAATTATCTTTACTTCCtt                     >  2:44113/1‑151 (MQ=255)

                                                                                                                                   cccccTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCATTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCtt                     >  6:1160487/1‑151 (MQ=255)

                                                                                                                                    ccccTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTGCCCCCCCGCGCACCAATTATGTTTACTTGCttt                    >  7:26372/1‑151 (MQ=255)

                                                                                                                                    ccccTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCttt                    >  2:1196736/1‑151 (MQ=255)

                                                                                                                                     cccTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCGTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCgttg                   >  6:1067200/1‑150 (MQ=255)

                                                                                                                                      ccTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGGTCCAGTGCCCCCCCTCGCCCCAATTATCTTTACGTACGTTCt                  >  6:1415286/1‑151 (MQ=21)

                                                                                                                                       cTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGCCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCGTTCtt                 >  1:1574511/1‑151 (MQ=255)

                                                                                                                                       cTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGGTAAAGTGCCCCCCCGCGCCCCAATAATCTTTAATTccggtggt                 >  5:415085/1‑145 (MQ=21)

                                                                                                                                        tC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTg                >  4:684463/1‑151 (MQ=255)

                                                                                                                                        tC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCttttttt                >  3:1908598/1‑150 (MQ=255)

                                                                                                                                        tC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCGCGCACCAATTATCTTTACTTGCTTTCTTg                >  6:814183/1‑151 (MQ=255)

                                                                                                                                        tC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGT‑CCCCCCCGCGCACCAATTATCTTTACTTCCTTTCTTGt               >  2:1789205/1‑151 (MQ=255)

                                                                                                                                         c‑GCACCAAACGAGTAGATATTAAAAATCGTAAGATGCCTTTCCGCAGGTTGCGGCTTTCGTCGAGGGGATAGGTGCTGGCTTGCGTTCCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGt               <  5:1752200/151‑1 (MQ=12)

                                                                                                                                             aCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCATGtttg            >  2:399902/1‑150 (MQ=255)

                                                                                                                                             aCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGGCCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTGtgtt            >  5:506949/1‑148 (MQ=255)

                                                                                                                                              ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCCAGTGCCCCCCCGCGCCCCAAGTATCTTTACGTCCGTGCTTGTttct           >  3:992489/1‑151 (MQ=21)

                                                                                                                                              ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttct           >  6:1338811/1‑151 (MQ=255)

                                                                                                                                               cAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTGCTTTCTTGTttctt          >  4:195357/1‑151 (MQ=255)

                                                                                                                                               cAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTAAATCCTTTCTTGTttctt          >  2:422237/1‑151 (MQ=255)

                                                                                                                                                aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGTCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTGCTTACGGACGT‑GGGGGTTCTAGTCCCGCCCCGCGCACCAATTATCTTTACTTACGTTGTTGtttttt          >  7:2053996/1‑150 (MQ=21)

                                                                                                                                                aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttcttc         >  3:1084228/1‑151 (MQ=255)

                                                                                                                                                aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttcttc         >  3:1523189/1‑151 (MQ=255)

                                                                                                                                                aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAACTCCCCCCCCGCGCCCCAATTATCTTTACGTCCGTTGTTGGttcttg         >  8:626640/1‑150 (MQ=21)

                                                                                                                                                    gAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTCCTGTCTTGTTTCTTCCTTg     >  3:776238/1‑151 (MQ=255)

                                                                                                                                                    gAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCttt     >  4:1870126/1‑150 (MQ=255)

                                                                                                                                                    gAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATATTTACTTACTTTCTTGTTTCTTCCttt     >  4:1948328/1‑150 (MQ=255)

                                                                                                                                                     aGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTGAAGTCCCCCCCCTCGCCCCAATTATATTTTCTTACTTTCTTGTTTGTTACTTg     >  6:796561/1‑150 (MQ=21)

                                                                                                                                                     aGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTGAACTCCCCCCCCTCGCACCAATTATCTTTACTTGCTTTGTTGTTTCTTCGTTgt    >  2:1338887/1‑150 (MQ=255)

                                                                                                                                                     aGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTg     >  5:345093/1‑150 (MQ=255)

                                                                                                                                                     aGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCACCCACCAATTATCTTTACTTACTTTGTTGTTTGTTGCTTg     >  2:662167/1‑150 (MQ=21)

                                                                                                                                                     aGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGGTGAAGGGCCCCCCCGCGCCCCCATTATCTTTAGGTGCTTTGTTGTTTGTTGCTTgt    >  2:1653822/1‑150 (MQ=18)

                                                                                                                                                       gCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGAtt  >  1:1919249/1‑151 (MQ=255)

GGGGGTTCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTG‑GGGGTTCAAGTCCCCCCCCTC‑GCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGT‑GGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATT  >  DH1/3906687‑3906986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`