BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	936,169	0	T	.	36.0%	260.1 / 154.5	119	intergenic (‑13/+21)	ECDH1_RS04615/ECDH1_RS04620	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand): ref base T (5/68); new base . (8/33); total (15/104)
Fisher's exact test for biased strand distribution p-value = 6.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

GAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTA  >  DH1/936019‑936297

gAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                                    <  5:2044939/151‑1 (MQ=255)

 attGGTGCTTTTTTATTGGAGACCAGAAACAAAAAAAGACCTGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                   <  4:1068017/149‑1 (MQ=255)

 aaTGGGTCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                   <  4:1614469/151‑1 (MQ=255)

 aaTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                   <  5:1682555/151‑1 (MQ=255)

 aaTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                   <  7:1764367/151‑1 (MQ=255)

  atgtctcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGAGGTGGCCGGATTTGAACCGACGTCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                  <  2:492954/145‑1 (MQ=255)

  aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGTATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCACGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                  <  5:538628/151‑1 (MQ=255)

  aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                  <  6:1937722/151‑1 (MQ=255)

  aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                  <  2:1931850/151‑1 (MQ=255)

    ggttcTTTTTTATGGGAGACCAGAAAAACAAAAAGAGCTTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttta                                                                                                                                 <  8:602630/146‑2 (MQ=17)

    ggggcTTTTTTTTGGGAGACCTGTAACAATAACTGAGGTGTTTGGTTGTTCTATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttta                                                                                                                                 <  6:1737099/147‑2 (MQ=11)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACCCCCGTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACAGTACTGCtttt                                                                                                                                 <  1:1721862/150‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCGATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                <  3:1841397/151‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                                 <  6:495714/150‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                                 <  8:25978/150‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                <  2:324042/151‑1 (MQ=255)

    ggCGCTTTTTTATGGGAGACCACCAAAAAAAAAACAGCCTTTAGGTTGTTCATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttta                                                                                                                                 <  7:828209/150‑2 (MQ=17)

     gcgcTTTTTTTTGGGACACCAGAAACAAAAAAACACCCGTTAGGCTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttact                                                                                                                               <  1:1578229/151‑4 (MQ=255)

     gcgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGGGGGGGCCGGAGTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCGGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTAc                                                                                                                               <  7:1669484/151‑1 (MQ=255)

      cgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACt                                                                                                                              <  3:371054/151‑1 (MQ=255)

      cgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACt                                                                                                                              <  3:1736561/151‑1 (MQ=255)

       gcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACtt                                                                                                                             <  7:1781443/151‑1 (MQ=255)

        cgtttttATGGGAGACCACAAACAAAAAAACCCCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtca                                                                                                                           <  5:156052/149‑1 (MQ=255)

        catttttATTGGAGACCAGCTAAAAAAAAACCATCGTTATGGTGTTCAATAATTGGTTGCGGGGGCCCTATTTGAACCCACTACCTTCGTGTTATGATCCCGACGATCGACCAGGCTGATCCACCCCGCGTCACCGTACTGC‑TTTACTtc                                                                                                                            <  4:1733656/148‑1 (MQ=9)

         ttttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttaattaa                                                                                                                            >  2:1960929/1‑144 (MQ=255)

          tttttATGGGAGACCTGAAACAAAAAAACACCCTTTAGGTTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcat                                                                                                                          <  2:1897928/150‑1 (MQ=17)

          tttttATGGGAGACCAGAAAAAAAAAAAGAGTGGTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtca                                                                                                                           <  2:1669427/149‑1 (MQ=17)

          tttttAAGTGAGACCAGTTACAATAAAACAGTGGTATGGTTGTTAAATATTTTGTTGCGGGGGCCGGATTTGAAGCGACGACCTTCGGGTTATGAGCCCGACTAGCTACCAGGCTGCTCCACCCCGCGTCACCTTACTGC‑TTTACTtcat                                                                                                                          <  3:106753/150‑1 (MQ=11)

           ttttATGGGAGACCAGAAACAAAAAAAGACGCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcat                                                                                                                          <  4:717716/150‑1 (MQ=255)

           ttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcat                                                                                                                          <  4:191111/150‑1 (MQ=255)

           ttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcat                                                                                                                          <  8:163357/150‑1 (MQ=255)

           gtgtATGGGAGACCCGCACCCACAAAACACCCGTGAGGGTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcatc                                                                                                                         <  1:537102/147‑1 (MQ=14)

            tttATGGGAGACCGGAAACAAAAAAAGACCCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcatc                                                                                                                         <  6:52910/149‑1 (MQ=255)

            tttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttactatatcgg                                                                                                                        >  8:1237390/1‑141 (MQ=255)

            tttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatc                                                                                                                         <  1:30147/150‑1 (MQ=255)

            tttATGGGAGACCACAAACAAAAAAACACCCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttact                                                                                                                               <  7:1238001/144‑4 (MQ=255)

            tttATGCGAGACCAGAAACAAAAACACACCCTTTAGGGTGTTCATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTTTGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcatca                                                                                                                        <  5:1389455/150‑1 (MQ=17)

             ttATTGGAGACCAGTACCAAATAAACACGCTTTATGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTCACTTCATCaa                                                                                                                       <  7:750080/150‑1 (MQ=14)

             ttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatca                                                                                                                        <  2:1956970/150‑1 (MQ=255)

              tATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                                       <  7:320762/150‑1 (MQ=255)

              tATGGGACACCAGAAACAAAAAAACAGCCGTTAGGGTGTTCTATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCaa‑a                                                                                                                     <  1:1961137/150‑1 (MQ=255)

               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAA‑A‑tt                                                                                                                  >  4:907309/1‑151 (MQ=255)

               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGGTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑t                                                                                                                   <  3:1227236/151‑1 (MQ=255)

                ttggAGCCCAGAAAAAAAAAAACACCGTTTTGGGTGTTAAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAA‑A‑tt                                                                                                                  <  6:867401/148‑1 (MQ=14)

                 gggacaagattaaCAAAAAAACACCCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGTTTTGAACCGACGACCTTCGGGTTATGTGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTGAACGTTCTTGCTTTACTTCATCAA‑A‑ttt                                                                                                                 <  4:1674137/140‑1 (MQ=17)

                 gggAGACCAGCAACAAAAAAACACCCGTGAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATAAA‑A‑ttt                                                                                                                 <  1:1459349/150‑1 (MQ=255)

                 gggAGACCAGACACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑ttt                                                                                                                 <  4:66898/151‑1 (MQ=255)

                 gggAGACCAGAAACAAAAAACCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑ttt                                                                                                                 <  4:1861026/151‑1 (MQ=255)

                 gggAGACCAGAAACAAAAAAAGACGCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGAGGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑tt                                                                                                                  <  5:1222229/150‑1 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑ttt                                                                                                                 <  6:42016/151‑1 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑tt                                                                                                                  <  8:2024654/150‑1 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑tt                                                                                                                  <  7:155821/150‑1 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑tt                                                                                                                  <  5:1807169/150‑1 (MQ=255)

                 gggAGACCAGAAAAAAAAAAAGAGCTTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAA‑A‑ttt                                                                                                                 <  4:1740763/150‑1 (MQ=14)

                  ggAGTCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTg                                                                                                                <  7:736560/151‑1 (MQ=255)

                  ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTg                                                                                                                <  3:1599314/151‑1 (MQ=255)

                  ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTg                                                                                                                <  5:1957517/151‑1 (MQ=255)

                  ggAGACCAGAAAAAAAAAAACCGCCTTAAGGGTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAA‑A‑tttta                                                                                                               <  5:566946/151‑3 (MQ=14)

                   gacccctccaaCAAAAAAACCCCCTTTAGGTTTTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAA‑A‑ttttaa                                                                                                              <  2:2042792/142‑4 (MQ=11)

                    agaCCAGAAACAAAAAAACAGCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGAt                                                                                                              <  4:1092112/151‑1 (MQ=255)

                    agaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGAt                                                                                                              <  2:164783/151‑1 (MQ=255)

                    agaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGAt                                                                                                              <  7:1352752/151‑1 (MQ=255)

                    agaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGAt                                                                                                              <  7:491942/151‑1 (MQ=255)

                     gaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGAtt                                                                                                             <  5:151313/151‑1 (MQ=255)

                      aCCAGAAACAAAAAAACACCGGTTTTGGTGTTCATTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAA‑A‑ttttaattg                                                                                                           <  4:702664/151‑7 (MQ=11)

                      aCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTg                                                                                                            <  3:1403193/151‑1 (MQ=255)

                       cccccaccAAAAAAACCCCCTTCTGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAA‑A‑ttttaattgg                                                                                                          <  1:563253/144‑8 (MQ=11)

                         aGAAACAATAAAACACCCGTTTTGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAA‑ATTTTAATTGGtt                                                                                                         <  6:1067201/151‑1 (MQ=11)

                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGtt                                                                                                         <  6:2039270/151‑1 (MQ=255)

                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGtt                                                                                                         <  4:434199/151‑1 (MQ=255)

                          gAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGTTg                                                                                                        <  2:254821/151‑1 (MQ=255)

                          gAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGTTg                                                                                                        <  6:1563874/151‑1 (MQ=255)

                          gAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACCGCTTTTACTTCATCAA‑A‑TTTGATTGGTTg                                                                                                        <  7:770499/151‑1 (MQ=255)

                          gAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTAGGGTTATGAGCCCTACGAGCTACAAGGCTGATCCACCACGCGTCAACTTACTGCTTTTAGTGATGAAA‑A‑TTTGATTGGTTg                                                                                                        >  2:687832/1‑151 (MQ=18)

                          caaacacaaacacAGCGTTCAGGGTGTTAAATAATTGGTGGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAA‑ATTTTAATTGGTTg                                                                                                        <  1:1262660/150‑1 (MQ=11)

                           taaaaaataatCACCCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAA‑A‑tttta                                                                                                               <  6:1033500/138‑3 (MQ=14)

                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGTTGc                                                                                                       <  2:298225/151‑1 (MQ=255)

                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTAATTCATGAA‑A‑ttttaagatcgta                                                                                                       >  5:1034391/1‑140 (MQ=17)

                            aaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttta                                                                                                                                 >  3:485050/1‑125 (MQ=255)

                            aaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGTTGCg                                                                                                      <  7:1004441/151‑1 (MQ=255)

                            aaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGTTGCg                                                                                                      <  8:1750368/151‑1 (MQ=255)

                            aaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGTTGCg                                                                                                      <  7:1642668/151‑1 (MQ=255)

                            aaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGTTGCg                                                                                                      <  1:89030/151‑1 (MQ=255)

                               aaaaaaaGACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCTGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGTTGCgggg                                                                                                   <  1:870103/151‑1 (MQ=255)

                               aaaaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGTTGCgggg                                                                                                   <  2:580904/151‑1 (MQ=255)

                                 aaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGTTGCGGGGGc                                                                                                 <  1:1471306/151‑1 (MQ=255)

                                   aaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACGTCATCAA‑A‑ttttaattg                                                                                                           >  4:357119/1‑132 (MQ=255)

                                     acacCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAA‑A‑tttt                                                                                                                <  5:1806987/131‑2 (MQ=255)

                                      cagccGTTTGGCTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcat                                                                                                                          <  4:1665802/119‑1 (MQ=11)

                                      cacCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttaattcat                                                                                                                           >  1:1518739/1‑115 (MQ=255)

                                      cacCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTACAACCCAAGACCTACCAGTACTGAAATAAATTCTTCAA‑A‑TTTGAATGTTTCCGGGGGCCGGAt                                                                                            >  8:465871/1‑151 (MQ=9)

                                        ccggTTCGGGTTTTCAAACTTTGGTTCCGGGGGCCGGACTCGCCCGCCCGCCGTTCGGTTAAACCGCCCGACGAGCTACCATTCTCCTCCACCCCCCCTAACATTACTGAGCATACTTCATCAA‑C‑TTTCATTGGTTGCGGGGGCCGGAttt                                                                                          <  3:1932768/148‑1 (MQ=2)

                                                             tGGTTGCGGGGGCCGGAATGTAGCCGACGCCGTTCGGGTTATTCGCCCGACGAGCTTCGATGCTTGTGGACCCCGCGTCAACGTACTGCTTTTACATCCTCAT‑A‑TTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGtt                                                                      <  7:73934/150‑1 (MQ=2)

                                                               gTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGACGGAAGAGCTTCGAGGATGCTAAAACGGTCTTCACCGTTCTGCATTTCGTTGTTCAA‑A‑TTTTATTTATTGAGAGGGCGGGAGTTGAGCGGACGGGCTTgggggtttg                                                                   >  6:237622/1‑146 (MQ=2)

                                                                  gCGGGGGCCGGAGTTGACCCGACGAGCTTGGTGTTATGAGCCCGCAGAGCGACCAGTCTCCTCCACCCCCCGTCACAGTACTGCGTTTACTTCATGAA‑A‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAg                                                                 <  3:672642/150‑1 (MQ=11)

                                                                                          acCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTAGTTCATAAA‑T‑TTTGATTGGTTGCGGGGGCCGGATTTGAACcga                                                                                   >  7:148426/1‑108 (MQ=255)

                                                                                                             gacgaGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTAATCAA‑A‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacga                                                         >  4:149275/1‑115 (MQ=255)

                                                                                                                    tACCAGGCTGCTCCACCCCGCGTCACGGTAGTGC‑GTTAATTCATCAATT‑TTTAATTCGTTGCGCGTGCGGTATTAGACCCTACGACATTGGGCTTAATAGACCGCCGACATACAAGGCTGCTCCCCCAAGCGTCaggcgattgggcctat               >  5:1722657/1‑135 (MQ=9)

                                                                                                                                 cACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTAATGCTATACTTCATCAAAatttt  >  3:330506/1‑149 (MQ=255)

                                                                                                                                   ccccGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGt                                >  1:44973/1‑118 (MQ=255)

GAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAA‑A‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTA  >  DH1/936019‑936297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`