BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	936,169	0	T	.	35.1%	259.1 / 155.6	118	intergenic (‑13/+21)	ECDH1_RS04615/ECDH1_RS04620	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand): ref base T (3/71); new base . (5/35); total (8/110)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-01

Only 100 of 144 total aligned reads displayed.

GAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTG  >  DH1/936019‑936305

gAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                                           <  6:973576/151‑1 (MQ=255)

 attggttcgtttttATGGGAGACCTGAAAAAAAAAATGATTTTTTTGGTTGTTCTATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                          <  4:2259498/142‑1 (MQ=14)

 aaTGGGGCTTTTTTTTGGGAGCCCTGTAACAATAAATGACCCGTTTGGTTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                                           <  4:1998512/150‑1 (MQ=17)

 aaTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                          <  3:1765586/151‑1 (MQ=255)

 aaTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                          <  5:1527953/151‑1 (MQ=255)

  aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAAGACGCTTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                         <  2:2004255/151‑1 (MQ=255)

  aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                         <  2:227882/151‑1 (MQ=255)

  aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                         >  6:1720441/1‑151 (MQ=255)

   atgctcTTTTTTATTGGAGACCACAAAAAAAAAAACACCCTTAATCGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACAGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttta                                                                                                                                        <  1:1323903/146‑2 (MQ=17)

   agccgcTTTTTTATGGGAGCCCAGCTACAAGCAAACCCCCTTCAGGCTCTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                         <  5:2248173/147‑1 (MQ=14)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttta                                                                                                                                        <  6:1514795/150‑2 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttta                                                                                                                                        <  4:531951/150‑2 (MQ=255)

    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                       <  1:1684189/151‑1 (MQ=255)

    ggCGCGTTTTTATGGGAGCCCTGAAAAAAAAAAACACTCGTTAGGCTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttta                                                                                                                                        <  1:1309023/150‑2 (MQ=18)

     gcgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTAc                                                                                                                                      <  4:85065/151‑1 (MQ=255)

     gcgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTAc                                                                                                                                      <  6:23755/151‑1 (MQ=255)

      gtcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGTTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttactt                                                                                                                                     <  8:1149510/149‑5 (MQ=255)

      cgcgTTTTTATGGGACACCAGCAACAAATAATCACCCGTGATGGTGATCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttactt                                                                                                                                     <  7:1738807/151‑5 (MQ=17)

      cgcTTTTTTATGGGAGACCAGAAACAAAAAACCACCCTTTAGGGTTTTAAATAATTGGTTTCGGTGTCCGGATTTGAAACGACGACCTTCGGTTTATGAGCCCGACGAGCTACGAGGCTGCTCCACCCCGAGTCAAAGTACTGCTTTTag                                                                                                                                      <  6:587337/150‑2 (MQ=255)

      cgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACt                                                                                                                                     <  8:1358671/151‑1 (MQ=255)

      cgcTTTTTTAAGGGAGCCCACAAACAAATAAACCCCCGTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttactt                                                                                                                                     <  5:1317974/151‑5 (MQ=17)

       gcctggttACGGGAGACCCGAAACAATAAAACCCCCTATAGGGTGTTCTATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtc                                                                                                                                   <  7:1446916/145‑1 (MQ=14)

       gcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACtt                                                                                                                                    <  1:1455446/151‑1 (MQ=255)

       gcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACtt                                                                                                                                    <  3:989579/151‑1 (MQ=255)

          ttttttTGGGAGACCAGAAACAACAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtca                                                                                                                                  <  6:1761594/149‑1 (MQ=255)

          tttttATGGGAGACCAGAAACAAAAAAACCCCCGTCAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtca                                                                                                                                  <  1:1612418/149‑1 (MQ=255)

          tttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtca                                                                                                                                  <  7:438565/150‑1 (MQ=255)

          tttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtc                                                                                                                                   <  2:2271913/149‑1 (MQ=255)

          tttttATGGCAGACCAGAAACAAAAAATCAGCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtca                                                                                                                                  <  4:515194/149‑1 (MQ=255)

           ttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttacatcata                                                                                                                                 >  4:2001493/1‑142 (MQ=255)

             ttATGGGAGACCAGAAACAAAAAAACACCGTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCaa                                                                                                                              <  6:735517/150‑1 (MQ=255)

             ttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatca                                                                                                                               <  8:1804785/150‑1 (MQ=255)

             tcttGGGAGACCCGATAAAAATAAACACCGGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACTACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTACATCaa                                                                                                                              <  1:475446/147‑1 (MQ=17)

              tATGGGATACCAGAAACAAAAAACCACCCGTTAGGGTGTTCAATAATTGGTGGCGGGGTCCGGATTTGAACGGACGAGCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                                              <  6:992258/150‑1 (MQ=18)

              tATGGGAGACCAGAAACAAAAAATGAGCCGTTAGGGTGTTCAATATTTGGTTGCGGGGGCCGGATTTGAACCGAGGACGTTCGGGTTATGAGGCCGACGAGCTACCAGGCTGCTCCACCGCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                                              <  8:411862/150‑1 (MQ=18)

              tATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGGGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                                              <  1:1894216/150‑1 (MQ=255)

              tATGGGAGACCAGAAACAAAAAAACACCCGTTAGGCTGTTCAATAATTGGTAGCGGGGCCCGGATTTGAACCGACGACCTTCGGGTTATGAGACCCACGAGCTACCACGCTGATCCACCCCGCCTCAACCTACTGCttttcataaaaaaa                                                                                                                              >  6:644706/1‑140 (MQ=18)

               ttgcgagaCCCGAACCACCAAAAGACCCTTAAGCGTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑t                                                                                                                           <  7:478571/146‑1 (MQ=11)

               aTGGGAGACCAGAACCAATAAAACCGCCTTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tt                                                                                                                          <  6:1146766/151‑1 (MQ=14)

               aTGGGAGACCAGAAACAAAAACACCGCGTTTTGGTTGATCATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑t                                                                                                                           <  1:88008/150‑1 (MQ=14)

               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaaa                                                                                                                             <  7:2184764/150‑1 (MQ=255)

               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑t                                                                                                                           <  7:2138737/151‑1 (MQ=255)

               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑t                                                                                                                           <  1:2139317/151‑1 (MQ=255)

               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑t                                                                                                                           <  5:661669/151‑1 (MQ=255)

                tGGGAGACCAGAAACAAATACAGCCCCTTGATGTTGTTCTATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tt                                                                                                                          <  4:795199/150‑1 (MQ=11)

                tGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCCGTACTGCtttactagatgaaatat                                                                                                                           >  2:474302/1‑137 (MQ=18)

                ggggAGCCCAGAAACAACAAAAGACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttt                                                                                                                         <  1:427545/150‑1 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCGGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttt                                                                                                                        <  5:1939751/151‑2 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCTAAAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttt                                                                                                                        <  3:880463/151‑2 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                         <  6:1410772/151‑1 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                         <  3:303475/151‑1 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                         <  3:1723703/151‑1 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑ttt                                                                                                                         <  2:1153489/151‑1 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttt                                                                                                                        <  2:1299576/151‑2 (MQ=255)

                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTGGCGGGGGCCGGAGTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑tt                                                                                                                          <  5:1515311/150‑1 (MQ=255)

                  ggAGACCAGTAAAAAAAAATGACGCGTTAGGTTGTTAATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttta                                                                                                                       <  4:319760/151‑3 (MQ=11)

                  ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCTTCAAA‑TTTg                                                                                                                        <  3:155552/151‑1 (MQ=255)

                  ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTg                                                                                                                        <  4:34382/151‑1 (MQ=255)

                  ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTg                                                                                                                        <  2:1010421/151‑1 (MQ=255)

                  ggAGACCACAAACAATAACACACACGTTATGGTCATCTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttta                                                                                                                       <  7:1074610/151‑3 (MQ=11)

                   tctaCCTGATACAATAAAAGACCCTTTATGTTGTTAAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑tttta                                                                                                                       <  6:354776/147‑3 (MQ=11)

                   gagaCCAGAAACCAAAACACACCCGTAAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaa                                                                                                                      <  5:1075248/151‑4 (MQ=14)

                   caccccAGAAACAAAAAAACACCCGTGAGGGTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaa                                                                                                                      <  3:2006615/147‑4 (MQ=14)

                     gaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGAtt                                                                                                                     <  3:827406/151‑1 (MQ=255)

                      ccccgatccAAAAAAACCCCCGATTTGGTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattg                                                                                                                   <  3:155835/143‑7 (MQ=11)

                      cccAGAAAAAAAAAAACCGCCTTTTGGGTGTTAATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGGCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattg                                                                                                                   <  5:1093835/150‑7 (MQ=11)

                      aCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTg                                                                                                                    <  1:1291542/151‑1 (MQ=255)

                      aCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTAATTAAA‑tttta                                                                                                                       >  5:355486/1‑145 (MQ=255)

                      aCCAGAAACAAAAAAACACCATTTAGTGTGTTCAATAATTGGTTGCGGTGGCCGGATTTTAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGAtt                                                                                                                     <  8:1596957/150‑1 (MQ=255)

                       ccAGTAACAATAAAACACAGTTTATGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattgg                                                                                                                  <  2:487075/151‑8 (MQ=11)

                       ccAGCAAAAAAAAATCCCGCGTGAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattgg                                                                                                                  <  4:582144/151‑8 (MQ=11)

                       ccAGAAACAACAAAACCCCCTTCAGGCTGATAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattgg                                                                                                                  <  5:1723673/151‑8 (MQ=11)

                       ccAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTgg                                                                                                                   <  4:249607/151‑1 (MQ=255)

                       ccAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTgg                                                                                                                   <  2:131/151‑1 (MQ=255)

                       ccAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTgg                                                                                                                   <  7:897420/151‑1 (MQ=255)

                        cAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaatt                                                                                                                    >  2:1640188/1‑143 (MQ=255)

                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGtt                                                                                                                 <  1:1679207/151‑1 (MQ=255)

                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGtt                                                                                                                 <  1:2076556/151‑1 (MQ=255)

                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGtt                                                                                                                 <  7:2228049/151‑1 (MQ=255)

                         aGAAACAAAAAAACACCCGTAAGGGTGTTCAATAATTGGTGGCGGGGGCGGGATTTGAACCGACGACCTTCCGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGtt                                                                                                                 <  2:869744/151‑1 (MQ=255)

                         aGAAAAAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGtt                                                                                                                 <  4:1421113/151‑1 (MQ=255)

                          gAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTg                                                                                                                <  8:1262232/151‑1 (MQ=255)

                           taaaaaaaaatCAGCCTTTAGGGTGTTCTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAA‑ttttaattgg                                                                                                                  <  8:1070373/143‑8 (MQ=11)

                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATGAAATTTTAATTGGtta                                                                                                                >  7:1072618/1‑149 (MQ=14)

                            aaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCg                                                                                                              <  8:487036/151‑1 (MQ=255)

                             aCAAAAAAACACCCGTTAGGGTGTTGAATAATTGGTTTAGGGGGCAGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACGTGCAAAAAA‑TTTAATTggatatgg                                                                                                             >  7:772707/1‑145 (MQ=17)

                             aCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCgg                                                                                                             <  2:303168/151‑1 (MQ=255)

                              cAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCggg                                                                                                            <  2:389897/151‑1 (MQ=255)

                               aaaaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCgggg                                                                                                           <  3:208797/151‑1 (MQ=255)

                                aaaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCggggg                                                                                                          <  5:210211/151‑1 (MQ=255)

                                 aaaaaCACCCGTTAGGGTGTTCAATAATTGGTGGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGc                                                                                                         <  1:2181962/151‑1 (MQ=255)

                                 aaaaaCACCCGTTAGGGTGTTCAATAATTGGTGGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGc                                                                                                         <  5:1372008/151‑1 (MQ=255)

                                  ataacaacCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGATCCGACGACCTTCGGTTTATGAGCCCGACGAGCTTCCAGGCTTCTCCACCCCGCGTCACCGTACTGATTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGcc                                                                                                        <  8:1364659/149‑1 (MQ=17)

                                  aaatCAGCCGTTTGGGTGTTCAATAATTGGTGGCGGGGGCCGGAGTTGCCCCGCCGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCTTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGcc                                                                                                        <  1:1873786/151‑1 (MQ=17)

                                  aaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTTATCAAATTTTTATTGGtttggtcggac                                                                                                        >  3:582830/1‑142 (MQ=14)

                                            gcaGGGTGTTCATTTATTTGTGGCGCGGGCCGGACTCGCCCGTACGCCCTTCGCGTTATCAGCCCGAACAGCTCCCAGTCTTCGGGAGCCCTCGTCAAACTACTCCCCTTACTTCATGATA‑ATTCATTGGTTGCGGGGGCCGGATTTGAAc                                                                                              <  5:1861916/149‑1 (MQ=255)

                                             tAGGGTGTTCATTAATTGGTTGCGGGGGCCGGATGGGTACCGCCTGCCTTCGGGTTATGAGCCCGACGAGCTTCCAGTCTTGTCGACCCCTCTTCAAAGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAAc                                                                                              <  7:878109/150‑1 (MQ=17)

                                                     tCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTtcat                                                                                                                                 >  4:1905466/1‑107 (MQ=11)

                                                                        gCCCGATTGGACCCCACGCCCTTCGCGTAATCCGCCCGACGAGCGCCCATTCTGCGCGACCCCCCGTCACCTTACAGCGGTTACATCATCATA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcgacg                                                                  <  7:894787/151‑1 (MQ=2)

                                                                                                                gaGCTACCAGGCTGCTCCACCCCGCGTCCGAGTACTGGTATTAGTAAAACAAA‑TTTGATTGGTTGCGGGGGGCGTATTACAACATACGACATTAGGGTTTTGATACAGAAGAGCGACAAGGCGGCACCACCCCCCTTCAcacaattcggcc                          >  3:748598/1‑140 (MQ=9)

GAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTG  >  DH1/936019‑936305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`