| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | DH1 | 613,597 | 0 | G | C | 24.5% | 350.8 / 44.5 | 247 | intergenic (+458/+565) | garK/yhaC | glycerate 2‑kinase/pentapeptide repeats‑related protein |
| Reads supporting (aligned to +/- strand): ref base G (61/93); new base C (1/49); total (69/178) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.76e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 248 total aligned reads displayed.
CCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATAC > DH1/613448‑613740 | cccGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa < 4:362678/151‑1 (MQ=255) ccGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa > 7:123747/1‑150 (MQ=255) ccGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa > 1:883501/1‑150 (MQ=255) cGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTCCTTTTGGAGGGGCAGaa > 1:757427/1‑150 (MQ=255) cGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCA‑G‑CTTCGGCGGGTTTTTGCTTTTGTAGGGGAAgta > 1:1579962/1‑148 (MQ=255) gTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAg < 4:210363/151‑1 (MQ=255) gaCTGAACCCGGTTAGGCGGGTTGAGGCAGTGACCGATTGATGGCCTAGACCCAAGACTCTACCCCACAGAAACCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGa < 3:1530967/150‑1 (MQ=14) aCTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATAGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CGTCTGAGGGTTTTTGCTTTTGGAGGGGCAGaa > 3:1466084/1‑147 (MQ=255) aCTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGATTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaa > 2:1150332/1‑147 (MQ=255) tGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CATCGGCGCCGTTTTGCGTTTGGAGGGTGAGATAGatg > 3:1253643/1‑150 (MQ=37) tGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAAACCCCTTATAAGTAAGTTTCACCTTATTTAAGTAAAAAACA‑G‑ATTCGGCATGTTTTTGATTTTGGAGGGGCAGAAAGatt > 2:1779602/1‑149 (MQ=25) gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaa < 4:594663/151‑1 (MQ=255) aaCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATGGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaa < 1:529516/150‑1 (MQ=255) aCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCC‑G‑CTTCGGAGGGTTATTGCTTTTGGAGGGGCAGAAAGatgaatg > 7:839904/1‑151 (MQ=255) aCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCACGCATATCCGTCAGTTTCACCTAATTTAAGTAAAAAACA‑G‑CTTCTCACGGTTTTAGCATTTTGAGTAAAATAAAGatgaat > 6:254560/1‑150 (MQ=255) ggTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGt < 7:1079649/151‑1 (MQ=255) gTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTc < 2:332295/151‑1 (MQ=255) aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatga < 4:901452/144‑1 (MQ=255) aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatga > 3:899400/1‑144 (MQ=255) aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCa < 1:212875/151‑1 (MQ=255) ggCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCac < 2:1328143/151‑1 (MQ=255) ggCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCa < 2:704109/150‑1 (MQ=255) ggctTGAGCCAGTGAGCGATTGCTGGGCTAGATGAATGACTGTCCACGACAGAACCAGGCTTATCGGTCAGTTTCACCTGATTTACGTAACAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgac < 4:1214785/150‑1 (MQ=255) gctTGAGCCTGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACATAAGCCTGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgacg < 2:53207/151‑1 (MQ=255) gctTGAGCCAGTGAGCGATTGCTGGGCTAGATGAATGACTTTCCACTACAGAACCAGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgac < 8:617372/150‑1 (MQ=255) gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacga < 7:869881/149‑1 (MQ=255) gctTGAGCCAGTGAGCGATTGCTGCCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGaggaatgccatgcacacacg > 1:823822/1‑138 (MQ=21) tGAGCCATTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCt < 1:987740/150‑1 (MQ=255) gcgCCAGGGAGCGATGGCGGGCCTAGATCAATGACTGCCCCCGACAGAACCCGGCTTAGGGGTCAGTTTCCCCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGTTTTTTGCTTTTGGAGGGGCACAACTATGAATGACTGTCCACGACGCta < 2:1212046/148‑1 (MQ=18) aGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCATTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCtata < 3:1308921/151‑1 (MQ=255) aGCCAGTGAGCGATGGCTGGCCTCGAGGAATGACGGCCCACGACACAACCCGGCTAATCGCGCAGTTGCCCCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTTGATGGGCAGAAAGATGAATGACTGTCCACGACGCtata < 2:991298/151‑1 (MQ=18) gCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACACGGCTTATCGGTCAGTTTCACCTGATTTACCTAAAAACCC‑C‑CATCGGAGGGGATGACCTATGAGAGCGGCTCAAAGAACAAAGACTGTGCACCACGCTAGAc > 1:1825248/1‑151 (MQ=19) cAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAACCGGCTTATCCGTCAGTTTCACCTGATTTACGTAAAAACCA‑G‑CTTCGGAGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCaagagcggatcccc > 7:1594891/1‑141 (MQ=18) tGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACACGGCTAATAGCTCACTTTCCCCAGATTTAACTAAAAACCA‑G‑CTTACGCAGGATTTTGGTTTTGGAGGGGCAGAAAGATGCATCACTGTCAACGCCGCTATACCCaa > 7:1485446/1‑150 (MQ=255) ggAGCGATTGCTGGCCTAGATTAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCaaa < 1:1069445/150‑1 (MQ=255) ttgcttttGCTGGCCTAGATTAATGACTGTCCCGCACAGAACCCGGCTTATCGGTCTGTTGCACGTGATTTACGTAAAACCCC‑G‑GTTCGGCGTGTTTTTGCTTTTGGCGGGGCATAAAGATGATTGAGTGTCCACGACGCTATACCCaaa < 3:85815/144‑1 (MQ=255) cgCGATTGCTGGCGTAGCTGAATGACTGGCCCCCACAGAACCCGGCTTAGCGGTCAGTTTCACGTGATTTACGTAAAACCCC‑G‑GTTCGGGGGGTTTTTGCTTTTGCAGGGGAACAAAGATTAATGCCTGTCCACGACGCTATACCCAaaag < 4:1635752/150‑1 (MQ=255) ggtttGCTGGCATATTTTAATGACTGGCCACGACAGAACCCGGGTTATCGGTCAGTTTCACCTTATTTACGTAAAAACCC‑G‑CTTCGGCGGTTTTTTGCTTTTGGTGGGGCAGAAACTTCAATGACTGTCCACGACGCTATACCCAaaagaa < 7:1685646/148‑1 (MQ=17) cGATTGCGGGCCTAGATTAATGACTGGCCACCACAGAACCCGGCTTATCGGTCAGTTGCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAaaagaa < 1:1408986/151‑1 (MQ=255) gATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGGAGAAAGATGAATGACTGTCCACGACTCTATACCCAaaagaa > 7:903152/1‑150 (MQ=255) aTTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgacg > 6:1698161/1‑134 (MQ=255) aTTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgacg < 5:1697215/134‑1 (MQ=255) ttgggggcgtattttaatgaCTGCCCACCCCACAACCCGGGTTTTCGGTCTTTTTCACGTTATTTACTTAAAAAGGC‑G‑CTGGGGGGGTTTTTTGTTTTTGTTGGGGCACAAAGTTTAATGAGTGTCCACGACGCTATACCCAaaagaaag < 5:93645/135‑1 (MQ=255) ttGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCATAAAAATCAATGACTGTCCACGAAGCTATACCCAaaataaatc > 2:743048/1‑149 (MQ=18) tGCTGGCCTAGATGAATGACGGGCCACGACACAACCCGGCTTATCGGTCAGTTTCACCTTTTTTACGTAAAACCCC‑G‑CTGCGGGGGGTTTTTGCTTTTGGAGGGGCACAAAGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGc < 5:1296358/150‑1 (MQ=17) gggCCTAGATGAATCACTTTCCACGACAGAACCCGGCTTAGCGGTCTGTTTCACCTGATTTACGTAAAAACCC‑G‑CTGCGGCGGGTTTTTGCTTTTGGTGGGGCAGAAAGATTAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCt < 6:1317738/150‑1 (MQ=17) ggCCTTGATGAATGACTGCCCACGACATAACCCGGCTTATCGGTCAGTTTCACGTTATTTAGTTAAAACCCC‑G‑CTTGGGGGGTTTTTTGGTTTTGGAGGGGCAGAAAGATGATTGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCt < 3:259852/150‑1 (MQ=17) catatatgaatgaCTGTCCACCACTTAACCGGGCTTCTGGGTCTGTTTCACCTGATTTACTTAAAAACCC‑A‑CTTGGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTAt < 4:749695/146‑1 (MQ=14) agaGATTAATGACTGGCCACCACAGAACCCGGCTTATCGGGAAGTTTCACCTGATTTACTTAAACACCC‑G‑CTTCGGCGGGTTTTTGGTTTTGGTGGGGCAGAAAGATGAATCACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATc < 3:1671934/149‑1 (MQ=17) aGATGAATGACTGTCCACGACACAAAACCCCTTATAAGTCATTTTAACCTGATTTACGTAAAAAACC‑G‑CTTCGCACGTTTTTTACTTTTGGAGGGGCAGAAAGATGAATGAATCTCAACGACACTATACCCAaaagaaaagcgcggaagcg > 8:479146/1‑139 (MQ=9) catcactgaCTGCCCACGACAGAACCCGGCTTTTCGGTCAGTTTCACCTGATTTACGTAAAACCCC‑G‑CTTCGGCGGTTTTTTGCTTTTGTAGGGGCAGAAAGATCAACCACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCgg < 4:1413973/144‑1 (MQ=17) attaatgaCTGTCCACTACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTAGTTAAAAACCC‑G‑CTTCGGCGGTTTTTTGCTTTTGTAGGGTCATAAAGATTAATTACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTc < 4:17517/148‑1 (MQ=17) gaatgaCTGCCCACCACACAACCCGGCTTATCGGTCAGTTTCACCTGATTTACTTAAAAACCC‑T‑CTTCGGCGGGTTTTTGCTTTTGGTGGGGCCCAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCa < 2:1637596/150‑1 (MQ=14) aaattcgtttaccaaaCATAACCCGTCTTATCGGTAATTTTAACCTGTTTTAGTTAAAAACCC‑G‑ATTTTGCGGGTTTTTGTTTTTGTTGGGGCTTAAATATGATTGACTTTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAg < 8:841009/136‑1 (MQ=2) aatcaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAg < 6:913444/150‑1 (MQ=255) gtgtatcgcaaaCAGAACCAGTCTTTTGGTGCAATTTCACGTGCTTAAGGTAAAACGGC‑T‑CTGCGGCGTGTTTTTGGTTTTGATGGTGGCCAAATTTGAATGACTTGCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGttt < 1:1547683/139‑1 (MQ=2) gaggggccaccaCAGAACCCGGCTTAGCGGTAATTTGCACCTGATTAACGTAAAACCCC‑G‑CTTCGGCGGGTTTTTGATTTTGGTGGGGCACAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGttt < 3:573614/144‑1 (MQ=14) gacgggccaccaCAGAACCCGGCTTAGCGGTCAGTTTCACCTGATTTACGTAAAACCGC‑G‑CTTCGGCGGGTTTTTGTTTTTGCAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGttt < 1:1278579/144‑1 (MQ=14) aCTGTCCACGACTTAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAACCCG‑G‑CTTCGGCGGGTTTTTGTTTTTGTAGGGGCTCATATATGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCa < 6:1787363/151‑1 (MQ=14) cctcctttaCAGAACCAGTCTTATCTGTCAGTTTCACTGTCTTTAGTTAAACCCGG‑G‑CTGCGGCGGGTTTTTGGTTTTGTTGGGTCCGAAATATGATTTACTGCCCACGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCaac < 8:1279177/143‑3 (MQ=2) tcccaccaCACAACCCGGCTTATCGCTCAGTTTCACGTTATTTACTTAAAAACCC‑T‑CTTCGGGGGGTTTTTGTTTTTGGCGGGGCTTAAATATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCAcc < 4:1832011/148‑1 (MQ=14) cgccACTACAGAACCCGGCTTAGGGGCCAGTTGCACGTCATTT‑CGTTAAAACCCGG‑CTGCGGCGGGTTTTTGCTTTTGCCGGCGCACAAAGATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCt < 2:454960/149‑1 (MQ=14) ccGACACAACCCGGCTTATCGGTCATTTTCCCCTGATTTAGTTAAAAACCG‑G‑CTTCGGCGGGTTTTTGTTTTTGGTGGGGCTCAACTATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGtt < 2:1490641/150‑1 (MQ=14) caacacAACCCGGCTTATGGGCAATTTGCCCGTCATTTACTTAAAACCGG‑T‑CTGCGGCGGGTTTTTGTTTTTGTTGGGGCACAATTATGAATGACTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGttt < 2:603763/149‑1 (MQ=9) gACAGAACCCGGATTATGGGTAATTTTCACCTGATTTACTTAAAAACCG‑C‑CTGCGGCGGGTTTTTGCTTTTGTAGGTGCCCAAAGATGAATGACTGTCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTa < 1:730214/151‑1 (MQ=14) aCAGAACCCGGCTTATCGGTCATTTTCACGTGATTTAGGTAAAAACCC‑G‑CTGCGGCGGGTTTTTGGTTTTGGCGGGGCATAAAGATGAATGACTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGttt < 7:765919/149‑1 (MQ=14) aCAGAACCCGGCTTATCGGTCATTTGCACCTGATTTAGTTAAAAACCC‑G‑CTGCGGCGGGTTTTTGCTTTTGGCGGGGCCCAAACATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGttt < 4:1663201/149‑1 (MQ=14) aGAACCCGGCTTATCGGTCAGTTTCTCCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGaa < 3:1075384/80‑1 (MQ=255) caaCCCGGCTTATCGGGCAGTTTCACCTGATTTACGTAAAAACCG‑G‑CTGCGGCGGGTTTTTGCTTTTGGAGGGGCACAAAGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTa < 3:737756/150‑1 (MQ=255) aaCCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGat > 5:776306/1‑82 (MQ=21) ccGGATTATGGGTCAGTTTCCCGTGATTTACTAAAAAACCC‑C‑CTTCGGGGGGTTTTTGGTTTTGCTGGCGCAGAAAGATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaaaa < 7:587333/151‑1 (MQ=11) ggctgatggctaactttCAGCTGATTTCGGTAACAACCG‑G‑CTGCGGCGGGTTTTTGCTTTTGTCGGGGCACAACTATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAcc < 6:1430403/137‑1 (MQ=11) ggCTTATCGGTCAGTTTCACCTGATTACCGTAAAACCCC‑G‑CTGCGGCGGGTTTTTGCTTTTGCAGGCGCAGAAAGATGAAGGACTGGCCACGACGCTATACCCAAACGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAcc < 7:1845602/151‑1 (MQ=11) gcgtATGGGACAGTTGCCCGTGATTTACGAAACACCCC‑G‑CTGCGGCGGGTTTTTGCTTTTGCCGGGGCCCAAACATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAccc < 1:1501531/148‑1 (MQ=11) tATCGGCAAGTTTCCCCTGATTTAGGTAACACCCC‑G‑CTTCGGCGGGTTTTTGCTTTTGTTGGGGCCGAACCATGATGGCCTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGc < 5:1188361/150‑1 (MQ=11) atgggaatgTTTCCCCTCATTACCTTAAAAACCC‑G‑CTGCGGCGGGTTTTTGCTTTTGCCGGGGCCTAAAGTTGATTGCCTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCtt < 6:666360/143‑1 (MQ=11) tcgttaattttccgatGATTTA‑GTTAAAACCC‑GTCTGCGGCGGGTTTTTGTTTTTGTAGGGGATTAATTATGAATTACTGGCCACGTCTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCtt < 2:495792/135‑1 (MQ=2) ggaaGTTGCACGTGATTTCCTTAAACACGG‑G‑CTGCGGCGGGTTTTTGGTTTTGTAGGGGCAAAAAGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggc < 1:338052/148‑1 (MQ=11) ttCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGCTGTACGTAAAAACCCGGTGCGGCGGGttt > 7:374853/1‑151 (MQ=255) tgccccTGATTTACGAAAAAACCC‑G‑CTTCGGCGGGTTTTTGGTTTTGCAGGGCCAGAAAGATGAAGCACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGG‑TTACGTAAAAACCCGCTTCGGCGGGttt < 3:416740/146‑1 (MQ=11) cACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGAGGAATGACGGTCCACCACCCTAGACCCAAAAGAAAGCGGCGTATCGGTCAGTATGACCTGGTGTACGTAAAAAACCGATGCGGCGGGtttcg > 5:1128102/1‑149 (MQ=11) ccgcaTTTACGTAACCCCCG‑G‑CGGCGGCGGGTTTTTGCGTTTGGCGGCGCACACAGATGCATGACTCTCCACGACGCTATACCCAACAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGc < 5:869559/147‑1 (MQ=11) gATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCCCGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACATGGTGTACGTAATAAACCGCTTCGGCGGGTTTGTGCttg > 7:1138773/1‑150 (MQ=11) aTTTCCGTAAAACCCC‑G‑CTGCGGCGGGTTTTTGCTTTTGGCGGCGCCGAAACATGAAGGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtttt < 5:80562/151‑1 (MQ=11) aTTTACGTAAACACCC‑G‑CTTCCGCGGGTTTTTGCTTTTGGAGCGGCCGAAAGATGAATGACTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCtttt < 1:579939/151‑1 (MQ=11) tttACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGGTGTACGTAATAACCCGGTCCGGCgggagttgggttg > 1:360543/1‑139 (MQ=11) tccgaaaaacCCC‑G‑CTGCGGCGGGTTTTTGCTTTTGGCGGGGCCCAAACATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTgg < 3:460829/145‑1 (MQ=11) ccGTAACACCCC‑G‑CTGCGCCGGGTTTTTGGTTTTGCCGGCGCCGAACAATGAAGGCCTCGCCACGACGCTCTACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAg < 1:1355550/150‑1 (MQ=11) aCGTAAAACCCC‑G‑CTGCGGCGGGTTTTTGCTTTGGTCGGGGCAGAACGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGa < 5:1517228/150‑1 (MQ=11) tacacacgc‑G‑CTTCGGCGGGTTTTTGCTTTTGTTGCTGCTGAAATATTATTGACTGTCCACTACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAggg < 2:256448/142‑1 (MQ=11) aaaaacCCC‑G‑CTGCGGCGGGTTTTTGCTTTTGCCGGGGCACAAAGATGAAGGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgggg < 5:1813225/150‑1 (MQ=11) acaaCCC‑G‑CTTGCTCGGTTTTTTGCTTTTGGAGTTTTATTTTTATGATTGTCCGTCCACGTCGCTATACACATACTTAATCTGGTTATCGGTAACTTTCAGCTGTTTTTCTTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGc < 6:580968/148‑1 (MQ=2) aaaaCCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGAAGGATCACTGGGCACGACGCGATACCCAAAACAAACGGGCTTATCGGTGAGTTTCCCCGGGTTTACGTAAATCCCGGCTTCGGGGGGTGTTGGCGTGGGGAgggggc > 5:580922/1‑149 (MQ=2) aaCCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGGAGAAAGATGAATGACTGTCCAAAACGCTATACCCAAAAGAAAGAGGATTATCGGTCAGTTTCACATATTTTAAGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgggggcgg > 6:964705/1‑147 (MQ=11) acccgtcttatgcggGTTTTTGTTTTTGTTGGTGCTTAATTTTGATTGACTTGCCAAGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa < 8:1068647/140‑1 (MQ=11) g‑CTGCGGCGGGTTTTTGCTTTTGTTGGGGCTTAAACTTGATTGACTTGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAg < 8:1403248/149‑1 (MQ=11) tCGGCGGGTTTTTGTTTTTGGAGGGGCATAAATATGAAGGACTGGCCACGACGCTATACCCAAAGGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatg < 7:1585724/149‑1 (MQ=11) ggTTTTTGCTTTTGCAGGGGCAGAAACATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTg < 5:236931/151‑1 (MQ=25) ttGGAGGGGCAGAAAGATGAATGACTGTCCACGACCCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTAAGATTGTGGAGGGCCCGTAAGACAAGAGCCAGTCCACGacagc > 8:1792311/1‑149 (MQ=17) aGGGGCAGAAAGATGAATGAATGTCCAAGACGCTATAACAAAAAGAAAGCGGATTATCGATCAGTTTAACATGGTTTACGTAAAAACAATATTATAAGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACGGTcggcagcgcgtggg > 6:466763/1‑138 (MQ=2) | CCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATAC > DH1/613448‑613740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |