Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,947,591	0	A	.	35.9%	218.8 / 126.1	92	intergenic (+18/‑16)	metW/metV	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (55/4);  new base . (30/3);  total (85/7)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.20e-01

CCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCC  >  NC_000913/2947453‑2947740                                                                                                                                             |                                                                                                                                                      ccgcggaaCCAATTACATTTTGTTGAATTCTCGGATTCCTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACgg                                                                                                                                           <  5:607190/145‑1 (MQ=14)           aaTTAAAATTTGTTGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATTACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCGGTACGGTGACGCgggg                                                                                                                                 <  7:1378446/151‑1 (MQ=255)                                                    tGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGag                                                                                                                            >  8:284830/1‑115 (MQ=255)                                                    tGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGag                                                                                                                            <  7:285281/115‑1 (MQ=255)                                                       agcagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                                                                                     >  6:620934/1‑151 (MQ=39)                                                                      cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTTATGAAGTaaaa                                                                                                                                                    >  4:351021/1‑73 (MQ=255)                                                                      cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTaaaa                                                                                                                                                    <  3:351047/73‑1 (MQ=255)                                                                                                      aaaTCCGGCCCCCGCAACCAA‑TG‑AATTATCATCAAGTAACAGCTGTAGGTTGAAGCGGGCTCGAGTAGCCACGAAGCACGTCGCGCACTTATAACGAAGGGCTGAGGGGCACCTCCGGCCCACGCAAACAATCATTGAACAcccaacaggc                                      >  7:752107/1‑144 (MQ=255)                                                                                                           cGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGGGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTGGTCGGTGCAACTCCGGCCCCCGCAACCAATTTTTGAACACCCTAACGGGAGttt                                 >  1:354373/1‑151 (MQ=17)                                                                                                             gCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGttttt                               >  2:37725/1‑151 (MQ=255)                                                                                                             gCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGttttt                               >  7:508197/1‑151 (MQ=255)                                                                                                             gCCCCCGCAACCAA‑TC‑AAATTTCATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGAAGCTCGTCGGGCTCATAACGCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGCTGtttgt                               >  3:58370/1‑149 (MQ=255)                                                                                                              cccccGCAACCAA‑TC‑AAATTTGATGAAGTAAACGCAGTACGGTGACGAGGGGTGGAGCAGACTGGTAGCTCGTCGGGCTCATAACCCGAAGGTAGTCGGTTCAAATCCCGCCCCCGCAACCAATTATTGAACACCCTACCGGGTGtttttt                              >  1:1179331/1‑151 (MQ=18)                                                                                                               ccccGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGttttttt                             >  4:1319340/1‑151 (MQ=255)                                                                                                                cccGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTg                            >  2:1615366/1‑151 (MQ=255)                                                                                                                 ccGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGt                           >  8:814599/1‑151 (MQ=255)                                                                                                                 ccGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACCGGTGTTTTTTTGt                           >  6:343414/1‑151 (MQ=255)                                                                                                                  cGCAACCAATTA‑AAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACACGCAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGAATGAACACGCTTTCTGGTGATTATTTggt                          >  7:1474665/1‑149 (MQ=11)                                                                                                                   ggctctgccgatctaTTTCATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGtt                          <  4:1225084/137‑1 (MQ=17)                                                                                                                   gCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGttttttt                             <  5:842222/147‑1 (MQ=255)                                                                                                                   gCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGttttttt                             >  6:843030/1‑147 (MQ=255)                                                                                                                   gCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGttt                         >  5:211253/1‑151 (MQ=255)                                                                                                                    cAACCAATTA‑AAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTATAGGCGGTGTTTTTGTTTc                        >  1:1508017/1‑151 (MQ=11)                                                                                                                    cAACCAATTA‑AAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGATTTATCACCCAATAGCGTGatttttgggatc                        >  6:1356382/1‑145 (MQ=11)                                                                                                                     aaCCAA‑TTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTTAACACCATCAAGGGGGTTTTTTTGtttgt                       >  6:824056/1‑149 (MQ=11)                                                                                                                     aaCCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCGGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCt                       >  1:288770/1‑151 (MQ=255)                                                                                                                     aaCCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGTACACCCTAACGGGTGTTTTTTTGTTTCt                       >  3:595918/1‑151 (MQ=255)                                                                                                                     aaCCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCt                       >  1:972538/1‑151 (MQ=255)                                                                                                                     aaCCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCt                       >  5:242486/1‑151 (MQ=255)                                                                                                                       ccAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATACCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTgg                     >  6:948128/1‑151 (MQ=255)                                                                                                                       ccAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTgg                     >  1:1533932/1‑151 (MQ=255)                                                                                                                       ccAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTgg                     >  8:1695263/1‑151 (MQ=255)                                                                                                                        cAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGCCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCAGAAGCTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACAACCTAACGGGTGTTTTTTTGTTTCTGGt                    >  6:234045/1‑151 (MQ=255)                                                                                                                        cAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGt                    >  1:756217/1‑151 (MQ=255)                                                                                                                        cAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGt                    >  5:1069624/1‑151 (MQ=255)                                                                                                                         caattaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTGATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTTAACACGCTATATGGTGTTTTTTTGGTGCTggg                    >  1:205251/7‑150 (MQ=11)                                                                                                                         caattaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTTAACAGCCTGACGGGTGTTTTTTTGTTTCTGGt                    >  7:1150751/7‑151 (MQ=14)                                                                                                                         aa‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCACCCAATTATTGAACACCCTAACGGGTGGTTTTTTGTTTGTGGtc                   >  4:1772397/1‑151 (MQ=255)                                                                                                                         aa‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGtc                   >  6:112556/1‑151 (MQ=255)                                                                                                                         aa‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGtc                   >  6:913777/1‑151 (MQ=255)                                                                                                                          aattaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACAAGCTAACGGGTGATTTAGTGTTTCTGGtc                   >  8:1746609/6‑151 (MQ=14)                                                                                                                          a‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGtct                  >  1:1513471/1‑151 (MQ=255)                                                                                                                           attaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAACTCCGGCCCCCGCAACCAATTATTGAACAACCAAACCGGTGTTTTTTTGGATCTGGtcg                  >  7:599789/5‑150 (MQ=14)                                                                                                                            ttaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTTAACACCCTATAGCGGGTTTTATTGTTTCTGGtgtg                 >  7:1435443/4‑148 (MQ=11)                                                                                                                            tC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGtctc                 >  1:1033296/1‑151 (MQ=255)                                                                                                                             taaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTTAACACCCTAAACGGTGATTTTTTGGTACTGGTCTcc                >  3:144528/3‑151 (MQ=14)                                                                                                                             taaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGATTGAACACCCAAACCGGTGTTTTTTGGTTTCgggggtcc                >  4:1286252/3‑146 (MQ=14)                                                                                                                             c‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTcc                >  4:1110226/1‑151 (MQ=255)                                                                                                                              aaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACAGGTGATTAGTTTTTTCTGGTCTccc               >  3:470800/2‑151 (MQ=14)                                                                                                                              aaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAATAACACCCTAAAGCTGGTTTAATTGTTACAGGTCTcc                >  3:1779001/2‑150 (MQ=1)                                                                                                                               aaaTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCACCCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTccc               >  4:30091/1‑151 (MQ=255)                                                                                                                                aaTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTccc               >  1:569854/1‑150 (MQ=255)                                                                                                                                aaTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTccc               >  1:1815951/1‑150 (MQ=255)                                                                                                                                aaTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTccc               >  6:1022334/1‑150 (MQ=255)                                                                                                                                aaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTTAACACCATAAAGGGTGTGTTTTTGTTTCTGGTGTCCCa              >  7:33139/1‑150 (MQ=17)                                                                                                                                 aTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCAt             >  3:523845/1‑151 (MQ=255)                                                                                                                                 aTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCAt             >  3:398257/1‑151 (MQ=255)                                                                                                                                 aTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGttttttt                             <  2:1844278/134‑1 (MQ=255)                                                                                                                                 aTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGttttttt                             >  1:1846555/1‑134 (MQ=255)                                                                                                                                 aTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAAAGGGTGATTTTTTGTTTCTGGTCTCCCAt             >  8:1329638/1‑150 (MQ=255)                                                                                                                                 aTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCATAACGGGTGTTTTTTTGtt                          >  3:1224917/1‑137 (MQ=255)                                                                                                                                  tttGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCAt             >  1:405456/1‑150 (MQ=255)                                                                                                                                  tttGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCAt             >  8:757677/1‑150 (MQ=255)                                                                                                                                  tttGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGttt                                 >  6:1396394/1‑129 (MQ=255)                                                                                                                                  tttGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTGTTTTTGTTTGGGGtctggaata            >  1:1799078/1‑144 (MQ=17)                                                                                                                                  tttGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAATTTAACACCCAAACGGGTGTTTTTTTGTTTCTGGTCTCCCATa            >  2:1153024/1‑150 (MQ=255)                                                                                                                                   ttGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATa            >  7:1177549/1‑150 (MQ=255)                                                                                                                                   ttGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAATTTAACACCAAAAAAGGTGTTTTATTTTTTCTGGTCTCCCATaa           >  3:576298/1‑150 (MQ=14)                                                                                                                                    tgatgaAGTAAAAGCAGTACGGTGACGGGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCGCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATaa           >  3:185339/1‑150 (MQ=255)                                                                                                                                    tgatgaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATaa           >  3:432459/1‑150 (MQ=255)                                                                                                                                    tgatgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAAAGGGTGTTTTGTGTTTTCTGGGCTCCCATaaa          >  1:378442/1‑150 (MQ=17)                                                                                                                                    tgatgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAATGAACACGCACAAGGCGGTTTAGTGGTTTGAGGTCTCCCATaac          >  1:387317/1‑149 (MQ=11)                                                                                                                                     gatgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGaagagc                                              >  4:969613/1‑109 (MQ=9)                                                                                                                                     gatgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACAcc                                              <  3:968670/113‑1 (MQ=25)                                                                                                                                     gatgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAATGATCAACATAAAGGCTCTGTTTTTTTTTCGGGTGTCCCaaaaaa         >  3:123591/1‑150 (MQ=11)                                                                                                                                      atgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCACCCAATTATTTAACACCCTAACGGCTCTTTTTTTGTATCGGGTCTCCCATaaaaac       >  2:1064141/1‑150 (MQ=17)                                                                                                                                      atgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACAGGTGATTTTTTGTTTCAGGTCTCCCATaaaaa        >  4:1040719/1‑150 (MQ=255)                                                                                                                                      atgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATaaaaa        >  3:1344999/1‑150 (MQ=255)                                                                                                                                       tgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGCGGGTTTTTTTGGTTCTGGTCTCCCATaaaaacg      >  3:469985/1‑149 (MQ=255)                                                                                                                                        gaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATaaaaa        >  8:1625128/1‑149 (MQ=255)                                                                                                                                        gaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATaaaaa        >  3:970828/1‑149 (MQ=255)                                                                                                                                        gaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTGTTTTTGTTGCGGGTGTCCCATAAAAAAgc     >  7:911309/1‑151 (MQ=18)                                                                                                                                         aaGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCACCCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATAAAAAAgc     >  4:1191812/1‑151 (MQ=255)                                                                                                                                         aaGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATAAAAAAgc     >  5:1380484/1‑151 (MQ=255)                                                                                                                                         aaGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATAAAAAAgc     >  8:1095409/1‑151 (MQ=255)                                                                                                                                          aGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTACCGGGTGTTTTTTTGTTTCTGGTCTCCCATAAAAAAgcg    >  7:978247/1‑151 (MQ=255)                                                                                                                                          aGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATAAAAAAgcg    >  2:416813/1‑151 (MQ=255)                                                                                                                                          aGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATAAAAAAgcg    >  7:470027/1‑151 (MQ=255)                                                                                                                                          aGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTGTTTTTGTGTCTGGTCTCCAATAAAAAAgcg    >  3:1006829/1‑151 (MQ=255)                                                                                                                                            tAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  4:1346434/1‑151 (MQ=255)                                                                                                                                            tAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  3:740737/1‑151 (MQ=255)                                                                                                                                            tAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  8:97565/1‑151 (MQ=255)                                                                                                                                            tAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAACCGAAGGTCGTCGGTTCAAATCAGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  5:223534/1‑151 (MQ=255)                                                                                                                                            tAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGCTAGCTCGTCGGGCTCATAACCCCAAGCTAGTCGCTTCAAATCCGGCACCCGCAACCAATTATTGAACACCCTAACGCGTGTTTTTTTGTTTCTAGTCTCCCATAAAAAAGCGcc  >  2:1150596/1‑151 (MQ=21)                                                                                                                                             aaaaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  1:921471/1‑150 (MQ=255)                                                                                                                                             aaaaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGGGTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  2:52338/1‑150 (MQ=255)                                                                                                                                             |                                                                                                                                                      CCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCC  >  NC_000913/2947453‑2947740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑