Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,947,591	0	A	.	27.5%	208.1 / 79.1	80	intergenic (+18/‑16)	metW/metV	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (57/1);  new base . (19/3);  total (76/4)
Fisher's exact test for biased strand distribution p-value = 6.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

GTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCC  >  NC_000913/2947523‑2947740                                                                       |                                                                                                                                                       gtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TA‑AAAATTTAAGAAATAAAAGCAGTACgg                                                                                                                                            >  1:1091546/1‑81 (MQ=11)                     gtcgtcGGTTCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTCAACAAGTAAAATCAGTAATGTTAAGAGGGGTCGAGAAGCAACGTAGCTCGTCGCGCTCATAACACGAAGGACGTAGGTTCAAATCCGGCCCCCGCAACaaaaaatttaa                                                   >  4:631446/1‑142 (MQ=9)                          cgGTTCAAATCCGGCCCCCGCAACCAA‑TG‑AAATATGAAGAACTAAATTCAGTACGGTGAAGCGGGCTCGAGCATACACGTAGATCGTCGGGCGCATAACCCTAAGGTTGAGGCTTCCCCTCCGGCCGCCGCCACCAATTAATCAACACCCt                                             >  7:622291/1‑151 (MQ=9)                             ttCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTCAAGATGTAAGAGCTGTAATGTGACGCGGGGTCGAGAAGAATCGAAGCTCGACGCGCTGATAAAGCGTAGCTCGGAGGGTCGAATCCCGCCCGCGCAACCAAATATTGAACACCCAAAc                                          >  5:389497/1‑151 (MQ=9)                                aaaTCCGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTTACGCGGGGTGGAGAAGCCTGGTAGCTCGTCGGGCGCATAAACCGAAGGTCTGGGGGGCAACTCCGGCCCCCGCCACCAATTATTGAACACCCTAAAggg                                       >  6:477518/1‑151 (MQ=17)                                  aTCCGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTCGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTAGTCGGTTCAACTCCGGCCCCCGCCACCAATTATTGAACACCCGAACGGgtg                                     >  8:491890/1‑151 (MQ=255)                                      ggCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGCGCAGACTGGTAGCTCGTCGCGCTCATAACCCGAAGGGCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAAAGGGTGtttt                                 >  5:134782/1‑151 (MQ=255)                                       gCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGtttt‑t                               >  7:1446151/1‑151 (MQ=255)                                         ccccGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGtttt‑ttt                             >  5:1242800/1‑151 (MQ=255)                                         ccccGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGtttt‑ttt                             >  5:1346452/1‑151 (MQ=255)                                         ccccGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGtttt‑ttt                             >  6:180006/1‑151 (MQ=255)                                         ccccGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGtttt‑ttt                             >  2:1321573/1‑151 (MQ=255)                                          cccGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTg                            >  7:429914/1‑151 (MQ=255)                                          cccGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTg                            >  8:675859/1‑151 (MQ=255)                                          cccGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTg                            >  6:1244396/1‑151 (MQ=255)                                            cGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGtt                          >  3:27403/1‑151 (MQ=255)                                            cGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACCGTCACGCGCGCTGGAGCAGCCTCGTACCTCGTCCGCCTCATAACCCGAAGCTCCTCGGTTCAAATCCCCCCCCCGCAACCAATTATTCAACACCCAAACgcgagaattttact                            >  3:55806/1‑137 (MQ=255)                                              cAACCAATTA‑AAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTTAACACCATAAAGGGTGTTTT‑TTTGtttg                        >  1:1391245/1‑150 (MQ=11)                                              cAACCAATTA‑AAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAATTTAACAACATAAAAGGTGtttt‑tttttatg                        >  4:1521157/1‑146 (MQ=11)                                              cAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTc                        >  5:1038528/1‑151 (MQ=255)                                              cAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTc                        >  7:840198/1‑151 (MQ=255)                                              cAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTc                        >  5:854003/1‑151 (MQ=255)                                              cAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTc                        >  5:840567/1‑151 (MQ=255)                                               aaCCAA‑TTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCTTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAATTTAACAACCTAAAGTGTCTTTT‑ATTGTTTc                        >  2:1464433/1‑150 (MQ=11)                                               aaCCAA‑TTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACAACCTAAAAGGTGTTTT‑ATTGTTTc                        >  3:38998/1‑150 (MQ=11)                                               aaCCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCt                       >  5:1366298/1‑151 (MQ=255)                                               aaCCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCt                       >  1:468810/1‑151 (MQ=255)                                               aaCCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCt                       >  7:1237218/1‑151 (MQ=255)                                                aCCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTg                      >  8:1406506/1‑151 (MQ=255)                                                 ccAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTgg                     >  5:524784/1‑151 (MQ=255)                                                 ccAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTCTTTT‑TTTGTTTCTgg                     >  8:1386095/1‑151 (MQ=255)                                                 accaattaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAGAACACCAAAAAAGGTGTTTTATTTGtgct                        >  4:759415/9‑147 (MQ=11)                                                  cAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCACCCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGt                    >  8:682875/1‑151 (MQ=255)                                                  cAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGt                    >  4:1325837/1‑151 (MQ=255)                                                  cAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGt                    >  7:356328/1‑151 (MQ=255)                                                  cAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGt                    >  3:724642/1‑151 (MQ=255)                                                   aa‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGtg                   >  1:1223370/1‑150 (MQ=255)                                                   aa‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGtc                   >  3:1071467/1‑151 (MQ=255)                                                   aa‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGGGTTTG‑TTTGTTTGTGGtc                   >  6:1056094/1‑151 (MQ=255)                                                    atttaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGtc                   <  2:541027/146‑1 (MQ=255)                                                    attcatattttGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTg                      <  1:1364408/140‑1 (MQ=255)                                                    aattaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTTAACACCATAACGGGTGTTTT‑TTTGTTTCTGGtg                   >  6:1041773/6‑150 (MQ=14)                                                    aattaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGATCACCCTGACGCGTCTTTT‑GTTGTTTCTGGtg                   >  5:583839/6‑150 (MQ=14)                                                    aattaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCATTGTACACCCTAACGGGTGTTTT‑GTTGtttggggtg                   >  6:668976/6‑145 (MQ=11)                                                    a‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGtct                  >  3:413541/1‑151 (MQ=255)                                                    a‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGtct                  >  3:950881/1‑151 (MQ=255)                                                    a‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTGTT‑TTTGTTTCTGGtct                  >  1:898388/1‑151 (MQ=255)                                                     attaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTATAGGCGGAGTT‑GTTGTTTGTGGtct                  >  8:376106/5‑151 (MQ=11)                                                      ttaaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACAACCTCACGGCGGTTTT‑GTTGTTTCTGGtg                   >  5:542592/4‑148 (MQ=14)                                                      tC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGtctc                 >  6:1423970/1‑151 (MQ=255)                                                      tC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGtctc                 >  3:1255610/1‑151 (MQ=255)                                                      tC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGtctc                 >  5:1212124/1‑151 (MQ=255)                                                      tC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAATTGAACACCCTAACGGGTGATTT‑TTTGTTTCTGGtctc                 >  4:2082/1‑151 (MQ=255)                                                       c‑AAATTTGATGAAGTAAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTCGCTCGTCGGGCTCATACCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCCATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGGCTcc                >  6:1402886/1‑151 (MQ=255)                                                       c‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTcc                >  7:154214/1‑151 (MQ=255)                                                        aaaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCCGTTCAAATCCCGCCCCCGCAACCAATTAAATAACAACAAAAAAGATGTTTT‑ATTGTTTCAGGTCTccc               >  3:464685/2‑151 (MQ=11)                                                          aaTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGGGTTTT‑TTTGTTTCTGGTCTccc               >  7:214890/1‑150 (MQ=255)                                                          aaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAATGAACAACCTAAAAGCTCATTA‑TTTTGTTCAGGTCTCCCa              >  4:1599843/1‑150 (MQ=11)                                                           aTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCAt             >  3:1496491/1‑151 (MQ=255)                                                           aTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCAt             >  3:766595/1‑151 (MQ=255)                                                           aTTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCAt             >  4:710695/1‑151 (MQ=255)                                                            tttGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCAt             >  7:899238/1‑150 (MQ=255)                                                            tttGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTTAACAACCTAAAGAGTGATTT‑TTTGTTTGTGGTCTCCCATa            >  4:861574/1‑150 (MQ=14)                                                            tttGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTCACGGGGGTTTT‑TTTGGTTCGGGGCTCCCCTa            >  8:1193992/1‑150 (MQ=14)                                                            tttGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGATAGAACAACCTAAACGGTGTGTT‑TTTGTTTCTGGGCTCCCATa            >  8:272974/1‑150 (MQ=14)                                                             ttGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCATa            >  1:1077166/1‑150 (MQ=255)                                                             ttGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCATa            >  5:412046/1‑150 (MQ=255)                                                              ttcatgaatAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCATCCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCATaa           <  6:39185/142‑1 (MQ=255)                                                              tgatgaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCATAACGCGTGTTTA‑TTTGAATCTGGTCTCCCATaa           >  4:442633/1‑150 (MQ=255)                                                              tgatgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTg                      >  8:1368779/1‑138 (MQ=255)                                                              tgatgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATAGAACAACCTAAAGCCTCATTT‑TTTTTTACTGGTCTCCCATaaa          >  4:821389/1‑150 (MQ=14)                                                               gatgaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCATaaa          >  4:1048015/1‑150 (MQ=255)                                                               gatgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCAGCAACCAATGATTGAACACGCACACTGCTCTTTA‑TGTTTTACTGGTCTGGCATaaaa         >  8:574793/1‑150 (MQ=11)                                                                atgaAGTAAAAGCAGTACCGTGACGCGGGGTTGAGCAGCCTGGTATCTCGTCGGGCTCATAACCCGAACGTCGTCGGTTCAAATCCCGCCCCCGCAACCAAATATTGAACACCCTAACGGGTGTGTT‑TTTTTTTCTGGTCTCCCATaaaa         >  4:1176705/1‑150 (MQ=21)                                                                atgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGtttt‑tt                              <  1:1333945/128‑1 (MQ=255)                                                                 tgaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCATaaaaa        >  2:16110/1‑150 (MQ=255)                                                                 tgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACACATCATTA‑TTTGTTTCTGGTCTCCCATaaaaa        >  3:1118854/1‑149 (MQ=17)                                                                  gaAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCATaaaaa        >  6:1529286/1‑149 (MQ=255)                                                                   aaGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAgc     >  1:208896/1‑151 (MQ=255)                                                                      tAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCTATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  6:126900/1‑151 (MQ=255)                                                                       aaaaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCATCCAATTATTGAACACACTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  1:987138/1‑150 (MQ=255)                                                                       aaaaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  8:394546/1‑150 (MQ=255)                                                                       aaaaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGcc  >  6:277652/1‑150 (MQ=255)                                                                       |                                                                                                                                                       GTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TC‑AAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTT‑TTTGTTTCTGGTCTCCCATAAAAAAGCGCC  >  NC_000913/2947523‑2947740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑