| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,831:1 | +G | 100% | pseudogene (251/261 nt) pseudogene (251/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,831 | 1 | . | G | 98.6% | 234.0 / ‑3.9 | 69 | pseudogene (251/261 nt) pseudogene (251/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (40/28); total (40/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
CGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTC > W3110S.gb/547762‑547896 | cgtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g < 1:3404683/71‑1 (MQ=255) cgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg‑gg < 1:7518171/71‑2 (MQ=255) cgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg‑g > 1:4404801/1‑70 (MQ=255) cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAAc > 1:3184785/1‑71 (MQ=255) gTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACtt > 1:6898249/1‑70 (MQ=255) ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCa > 1:583065/1‑67 (MQ=255) cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa < 1:769673/48‑1 (MQ=255) cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCtt > 1:4270000/1‑71 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTg > 1:2876220/1‑71 (MQ=255) aaGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGt > 1:504549/1‑71 (MQ=255) aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gg > 1:7238333/1‑47 (MQ=255) ggTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCa < 1:39252/71‑1 (MQ=255) ggTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCa > 1:4605997/1‑71 (MQ=255) ggTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCa < 1:1724504/71‑1 (MQ=255) tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCGCCa > 1:7933981/1‑71 (MQ=255) tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCa > 1:3537693/1‑71 (MQ=255) gCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGa < 1:582097/49‑1 (MQ=38) gCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGa < 1:3617416/49‑1 (MQ=38) cGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCAtgtg < 1:797001/71‑1 (MQ=255) cGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCAtgtg < 1:3361019/71‑1 (MQ=255) cGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCAtgt > 1:5921742/1‑70 (MQ=255) cGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGAGAAGTGAACTTCATTCCTTGTCACCAtgtg < 1:6063386/71‑1 (MQ=255) gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGaa < 1:4142000/48‑1 (MQ=38) gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGaa < 1:5077274/48‑1 (MQ=38) gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGaa < 1:1236655/48‑1 (MQ=38) aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCAtgt > 1:101206/1‑68 (MQ=255) aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAAt > 1:5536742/1‑71 (MQ=255) aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAAt < 1:3703023/71‑1 (MQ=255) aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATg > 1:6186211/1‑71 (MQ=255) aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGaa < 1:7344852/67‑1 (MQ=255) aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAAt > 1:1441996/1‑68 (MQ=255) tGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCg > 1:3885399/1‑71 (MQ=255) gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATg > 1:3855521/1‑67 (MQ=255) aGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCAtt < 1:5388332/46‑1 (MQ=38) aGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCg > 1:2064920/1‑71 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAAc < 1:7225166/39‑1 (MQ=37) cgcAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCtt < 1:1977406/48‑1 (MQ=38) cgcAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg < 1:5237695/70‑1 (MQ=255) gcAGGAGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc > 1:2414919/1‑71 (MQ=255) cAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc < 1:2875068/66‑1 (MQ=255) cAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc > 1:2725857/1‑70 (MQ=255) ggCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa > 1:2430764/1‑71 (MQ=255) ggCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa < 1:3619932/71‑1 (MQ=255) gcgcTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAAt > 1:7809436/1‑71 (MQ=255) cgcTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATg > 1:5681196/1‑71 (MQ=255) gcTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATgg > 1:5128617/1‑71 (MQ=255) tGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg < 1:1722262/70‑1 (MQ=255) ggCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg > 1:6179588/1‑71 (MQ=255) gCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:3377476/1‑50 (MQ=255) gCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:6988622/1‑50 (MQ=255) gCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:4521346/1‑50 (MQ=255) gCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:3208213/1‑50 (MQ=255) gCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGc > 1:7367344/1‑53 (MQ=255) cAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg > 1:3196243/1‑70 (MQ=255) cAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGt < 1:5314996/71‑1 (MQ=255) aaTACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTa > 1:4304963/1‑71 (MQ=255) tACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCAcc < 1:1662089/39‑1 (MQ=38) tACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCAcc < 1:6608481/39‑1 (MQ=38) tACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc > 1:7667677/1‑53 (MQ=255) tACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc > 1:7483499/1‑53 (MQ=255) tACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAtt < 1:1670846/71‑1 (MQ=255) cTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCAt > 1:5309585/1‑39 (MQ=38) cTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTa < 1:6399169/70‑1 (MQ=255) cTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc < 1:3944217/71‑1 (MQ=255) cTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc > 1:5357327/1‑71 (MQ=255) tGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:5180797/1‑43 (MQ=38) tGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATgg < 1:5336765/60‑1 (MQ=255) tGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATgg < 1:6266936/60‑1 (MQ=255) tGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATgg < 1:2085814/60‑1 (MQ=255) gAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATg > 1:7551715/1‑57 (MQ=255) gAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACt < 1:3498588/69‑1 (MQ=255) gAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc > 1:423441/1‑71 (MQ=255) gAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc > 1:1977199/1‑71 (MQ=255) gAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc > 1:3657858/1‑71 (MQ=255) | CGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTC > W3110S.gb/547762‑547896 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |