| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,353,829 | G→A | 12.9% | T26M (ACG→ATG) | yhcC ← | predicted Fe‑S oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,353,829 | 0 | G | A | 12.9% | 84.2 / 4.5 | 31 | T26M (ACG→ATG) | yhcC | predicted Fe‑S oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (9/18); new base A (2/2); total (11/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 | |||||||||||
ACAGAATGTGCAGCCACCACGCCCGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCTTATGCACCTTTTGCCCATAACGACGGGTGAGATCACCACCAAACATATTGACTAATTTCTGT > W3110S.gb/3353762‑3353897 | aCAGAATGTGCAGCCACCACGCCCGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGt > 1:109944/1‑69 (MQ=255) cAGAATGTGCAGCCACCACGCCCGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCa > 1:552781/1‑70 (MQ=255) cAGAATGTGCAGCCACCACGCCCGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCa < 1:150753/70‑1 (MQ=255) gAATGTGCAGCCACCACGCCCGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCt < 1:839039/71‑1 (MQ=255) aTGTGCAGCCACCACGCCCGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCtt < 1:256900/70‑1 (MQ=255) tgtgCAGCCACCACGCCCGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCTTAt > 1:477527/1‑71 (MQ=255) tgCAGCCACCACGCCCGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCt < 1:1637191/66‑1 (MQ=255) accacGCCCGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCTTATGCACCtttt < 1:1184993/71‑1 (MQ=255) accacGCCCGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCATCAGCTTATGCACCtttt < 1:1743527/71‑1 (MQ=255) acGCCCGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCTTATGCACCTTTTGcc > 1:766987/1‑71 (MQ=255) acGCCCGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCTTATGCACCTTTTGcc < 1:1654685/71‑1 (MQ=255) gCCCGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGGCAGCTTATGCACCTTTTGCCCa < 1:1765199/71‑1 (MQ=255) cccGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCtt > 1:526862/1‑54 (MQ=255) cccGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCATCAGCTTATGCACCTTTTGCCCAt < 1:1207812/71‑1 (MQ=255) gTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCTTATGCACCt < 1:349580/55‑1 (MQ=255) gTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCTTATGCACCTTTTGCCCATAacgacg < 1:1382686/71‑1 (MQ=255) tACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCTTATGCACCTTTTGCCCATAACGACgg < 1:1160808/71‑1 (MQ=255) gTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCTTATGCACCTTTTGCCCATAACGACGGGt > 1:433508/1‑69 (MQ=255) tCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCTTATGCACCTTTTGCCCATAACGACGGGTgag < 1:531214/71‑1 (MQ=255) gTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCTTATGCACCTTTTGCCCATAACGACGGGTGAGATcac < 1:1662450/71‑1 (MQ=255) gCTAAAACCGCCGTGCAGCGTCAGCTTATGCACCTTTTGCCCAt < 1:785931/44‑1 (MQ=255) cTAAAACCGCCGTGCAGCATCAGCTTATGCACCTTTTGcc > 1:1282173/1‑40 (MQ=255) tAAAACCGCCGTGCAGCGTCAGCTTATGCACCTTTTGCCCATaa > 1:632441/1‑44 (MQ=255) tAAAACCGCCGTGCAGCATCAGCTTATGCACCTTTTGCCCATAACGACGGGTg > 1:1281357/1‑53 (MQ=255) aaaCCGCCGTGCAGCGTCAGCTTATGCACCTTTTGCCCATAACGACGGGTGAGATCACCACCAAACata < 1:473683/69‑1 (MQ=255) aaaCCGCCGTGCAGCGTCAGCTTATGCACCTTTTGCCCATAACGACGGGTGAGATCACCACCAAACata < 1:66565/69‑1 (MQ=255) aCCGCCGTGCAGCGTCAGCTTATGCACCTTTTGCCCATAACGACGGGTGAGATCACCACCAAACATATTGa > 1:836225/1‑71 (MQ=255) gCAGCGTCAGCTTATGCACCTTTTGCCCATAACGACGGGTGAGATCACCACCAAACATATTGACTAAt > 1:584737/1‑68 (MQ=255) gCGTCAGCTTATGCACCTTTTGCCCATAACGACGGGTGAGATCACCACCAAACATAtt < 1:1104849/58‑1 (MQ=255) gCGTCAGCTTATGCACCTTTTGCCCATAACGACGGGTGAGATCACCACCAAACATATTGACTAATTTCTGt < 1:1685304/71‑1 (MQ=255) gCGTCAGCTTATGCACCTTTTGCCCATAACGACGGGTGAGATCACCACCAAACATATTGACTAATTTCTGt < 1:1030375/71‑1 (MQ=255) | ACAGAATGTGCAGCCACCACGCCCGATGGTACCGTCACGGTTAGGGCAGCTAAAACCGCCGTGCAGCGTCAGCTTATGCACCTTTTGCCCATAACGACGGGTGAGATCACCACCAAACATATTGACTAATTTCTGT > W3110S.gb/3353762‑3353897 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |