| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,831:1 | +G | 100% | pseudogene (251/261 nt) pseudogene (251/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,831 | 1 | . | G | 92.5% | 128.5 / 2.7 | 40 | pseudogene (251/261 nt) pseudogene (251/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (18/19); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
GCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑G‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACT > W3110S.gb/547761‑547894 | gcgtgcgtGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa < 1:2513400/71‑1 (MQ=255)gcgtgcgtGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGAAATACTGGAGCa < 1:2590288/71‑1 (MQ=255) gtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑g > 1:4569210/1‑71 (MQ=255) gcgcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTg < 1:4363194/71‑1 (MQ=255) cgcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGa < 1:4290344/71‑1 (MQ=255) cgcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑G‑G‑GGTAAGTGa < 1:4597/71‑1 (MQ=255) tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCa > 1:2265851/1‑71 (MQ=255) gTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g < 1:4251300/52‑1 (MQ=255) ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTc < 1:1776418/70‑1 (MQ=255) ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑g < 1:1685223/52‑1 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTg > 1:2014374/1‑71 (MQ=255) aaGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGt > 1:2027659/1‑71 (MQ=255) aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTc < 1:238300/71‑1 (MQ=255) gTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa < 1:278030/43‑1 (MQ=255) gTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa < 1:599407/43‑1 (MQ=255) gTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCAc < 1:1734562/71‑1 (MQ=255) gTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCAc < 1:744387/71‑1 (MQ=255) gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCa < 1:69128/70‑1 (MQ=255) gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCa < 1:2825504/70‑1 (MQ=255) gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCAt > 1:602803/1‑71 (MQ=255) cGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCAtgtg < 1:4069224/71‑1 (MQ=255) cGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCAtgtg < 1:3441416/71‑1 (MQ=255) aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCtt < 1:4622680/58‑1 (MQ=255) aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑G‑GCGG‑AAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:3754836/1‑71 (MQ=255) aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAAt > 1:348707/1‑70 (MQ=255) aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTCAATg > 1:3109136/1‑71 (MQ=255) gATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTc > 1:2541057/1‑52 (MQ=39) aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTc < 1:2396940/57‑1 (MQ=255) aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGcc < 1:433775/71‑1 (MQ=255) tGAAGCGCAGGCGCTGGCTATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGcc > 1:2707362/1‑70 (MQ=255) gAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:4870622/1‑65 (MQ=255) aaGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc > 1:4216309/1‑71 (MQ=255) aGCGCAGGCGCTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCg < 1:2056170/71‑1 (MQ=255) gcTGGCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCa > 1:5094776/1‑45 (MQ=38) gCAATACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg > 1:2640908/1‑71 (MQ=255) aTACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt < 1:2753406/71‑1 (MQ=255) tACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCa < 1:294237/59‑1 (MQ=255) tACTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg < 1:5140333/67‑1 (MQ=255) tACTGGAGCA‑GTG‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg < 1:4294683/67‑1 (MQ=255) cTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGcc > 1:2810891/1‑48 (MQ=255) cTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGcc > 1:175565/1‑48 (MQ=255) cTGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc < 1:4678957/71‑1 (MQ=255) tGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg < 1:3905491/64‑1 (MQ=255) tGGAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACt > 1:963000/1‑71 (MQ=255) ggAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa > 1:3886961/1‑56 (MQ=255) gAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa > 1:5106465/1‑55 (MQ=255) gAGCAGG‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa > 1:1763327/1‑55 (MQ=255) | GCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑G‑G‑GG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACT > W3110S.gb/547761‑547894 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |