| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,824,514 | T→C | 100% | pseudogene (647/687 nt) | rph → | ECK3633:JW3618:b3643; ribonuclease PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,824,514 | 0 | T | C | 100.0% | 181.7 / NA | 53 | pseudogene (647/687 nt) | rph | ECK3633:JW3618:b3643; ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/29); total (24/29) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGACGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑G‑GGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGC > W3110S.gb/3824446‑3824576 | aGACGGGCGCATCATTGAAGTGCAGGGGACGGAAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCt < 1:204601/71‑1 (MQ=255) aCGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCttg < 1:1159534/71‑1 (MQ=255) aCGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCttg < 1:4335651/71‑1 (MQ=255) aCGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCttg < 1:4223322/71‑1 (MQ=255) cGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCttgt < 1:2739852/71‑1 (MQ=255) gcgcATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTgg < 1:1299143/71‑1 (MQ=255) cgcATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGc < 1:1493717/71‑1 (MQ=255) atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGcgc > 1:3301282/1‑65 (MQ=255) atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc > 1:8617/1‑71 (MQ=255) atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc > 1:4699056/1‑71 (MQ=255) atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc > 1:364170/1‑71 (MQ=255) atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc > 1:3176598/1‑71 (MQ=255) atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc > 1:2977582/1‑71 (MQ=255) atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc > 1:2108754/1‑71 (MQ=255) atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc > 1:2343339/1‑71 (MQ=255) atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACACACCTTGTAGGCGCTGGc > 1:1043744/1‑71 (MQ=255) ttGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCttg < 1:4575865/59‑1 (MQ=255) ttGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCttg < 1:4433060/59‑1 (MQ=255) ttGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTg < 1:4091672/68‑1 (MQ=255) gAAGTGCAGGGGCCGGCAGACGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:5037967/70‑1 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:2408548/70‑1 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:3816372/70‑1 (MQ=255) aaGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCg < 1:2738937/70‑1 (MQ=255) cAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑ggg > 1:3721371/1‑70 (MQ=255) ggggACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑ggggaa > 1:1168623/1‑68 (MQ=255) ggACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑ggg > 1:3405667/1‑66 (MQ=255) aCGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCg < 1:4809717/59‑1 (MQ=255) aGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑gggg > 1:2085607/1‑59 (MQ=255) gAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCAt < 1:879039/71‑1 (MQ=255) ggCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGt < 1:3656726/71‑1 (MQ=255) gCGAGCCGTTCACCCATGAAGAGCTACTCACCttgt > 1:4137953/1‑36 (MQ=255) gCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCGGGCCCGA‑GTGGGAATCGAATCCATTGTAGCg < 1:1530798/70‑1 (MQ=255) ccGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCg < 1:857031/69‑1 (MQ=255) ccGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCg < 1:1945019/69‑1 (MQ=255) ccGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCg < 1:567631/69‑1 (MQ=255) ccGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGAc > 1:3987079/1‑71 (MQ=255) gTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTg > 1:2486123/1‑37 (MQ=38) ttCACCCATGAAGAGCTACTCACCTTGTTGGCGCTg < 1:2457734/36‑1 (MQ=37) ttCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCa < 1:5073320/71‑1 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGgaatcga > 1:1425469/1‑51 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGc > 1:1024424/1‑68 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAg < 1:344011/70‑1 (MQ=255) cACCAATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGc > 1:3730094/1‑68 (MQ=255) aTGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAggcg > 1:2811059/1‑71 (MQ=255) tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑gggga < 1:4557815/40‑3 (MQ=37) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCTAATCCATTGTAGCGACGCAGAAGgcggcg < 1:4102588/71‑1 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAGgcggcg > 1:2437565/1‑71 (MQ=255) gagCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCt < 1:1234289/71‑1 (MQ=255) agCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTg > 1:1049439/1‑71 (MQ=255) cTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAggcgg > 1:3666631/1‑64 (MQ=255) tACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAggc < 1:3083935/61‑1 (MQ=255) tACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGc < 1:377164/70‑1 (MQ=255) tCACCTTGTTGGCGCTGGCCCGAGG‑GGgaatcgaat > 1:2029082/1‑36 (MQ=19) | AGACGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑G‑GGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGC > W3110S.gb/3824446‑3824576 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |