| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 704,757 | G→A | 31.2% | R131H (CGC→CAC) | nagE → | fused N‑acetyl glucosamine specific PTS enzyme IICBA components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 704,757 | 0 | G | A | 31.2% | 65.4 / 38.7 | 48 | R131H (CGC→CAC) | nagE | fused N‑acetyl glucosamine specific PTS enzyme IICBA components |
| Reads supporting (aligned to +/- strand): ref base G (17/16); new base A (7/8); total (24/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.95e-01 | |||||||||||
GTGGCGCAGCCTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGTTACG > W3110S.gb/704688‑704816 | gtggCGCAGCCTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGc < 1:18808/71‑1 (MQ=255) cgcAGCCTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACACTTtg < 1:4509308/71‑1 (MQ=255) cgcAGCCTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACACTTtg < 1:4605581/71‑1 (MQ=255) gCCTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACACTTTGTGcc < 1:1683079/71‑1 (MQ=255) ccTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACg > 1:3184130/1‑61 (MQ=255) ccTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACg > 1:1376241/1‑61 (MQ=255) ccTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACg > 1:2665851/1‑61 (MQ=255) ccTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACg > 1:1684578/1‑61 (MQ=255) ccTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGcc < 1:3717884/70‑1 (MQ=255) ccTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCg < 1:3570225/71‑1 (MQ=255) ccGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACACt < 1:806031/56‑1 (MQ=255) ccGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACACTTTGTGCCGATTGCCa < 1:3924374/71‑1 (MQ=255) cGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCAc > 1:5142431/1‑71 (MQ=255) ttGGTCCTATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCg < 1:1373362/61‑1 (MQ=255) ttGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCg < 1:3758749/61‑1 (MQ=255) ttGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACACTTTGTGCCGATTGCCACCg > 1:4161154/1‑71 (MQ=255) ccGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACACTTTGTGCCGATTGCCACCGGAttc > 1:1639830/1‑71 (MQ=255) cGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGTCAAACGCTTTGTGCCgt > 1:1101123/1‑55 (MQ=255) cGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCg > 1:465899/1‑65 (MQ=255) gATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGc < 1:1847348/45‑1 (MQ=255) tatTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCt < 1:2485458/44‑1 (MQ=255) atTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGc < 1:1787041/42‑1 (MQ=255) atTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACACTTTGTGCCGa > 1:693595/1‑52 (MQ=255) atTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACACTTTGTGCCGa > 1:3653492/1‑52 (MQ=255) atTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACACTTTGTGCCGa > 1:1429319/1‑52 (MQ=255) ttAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCttt < 1:1141622/44‑1 (MQ=255) tAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCg > 1:1971369/1‑49 (MQ=255) tAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGc > 1:3009528/1‑54 (MQ=255) aaaCTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCtt > 1:1489195/1‑41 (MQ=255) aaaCTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCtt > 1:263427/1‑41 (MQ=255) aaaCTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCtt > 1:209400/1‑41 (MQ=255) aaCTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCt < 1:3800049/70‑1 (MQ=255) aaCTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACACTTTGTGCCGATTGCCACCGGATTCTTCTGCCt < 1:3180562/70‑1 (MQ=255) cTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGAtt < 1:1060346/48‑1 (MQ=255) cTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCAc < 1:3330389/53‑1 (MQ=255) cTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACACTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGt > 1:321426/1‑71 (MQ=255) tGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCAcc < 1:304556/53‑1 (MQ=255) gCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCg > 1:3041205/1‑53 (MQ=255) gCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCg > 1:3238983/1‑53 (MQ=255) ccTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGa > 1:1062949/1‑35 (MQ=255) ccTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTg > 1:577820/1‑61 (MQ=255) ccTGAGCTTCTTCGGCGGCAAACACTTTGTGCCGATTGCCAc > 1:46239/1‑42 (MQ=255) aGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCAtttt > 1:5140439/1‑71 (MQ=255) aGCTTCTTCGGCGGCAAACACTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGCCCAtttt < 1:229903/71‑1 (MQ=255) ggcggcAAACGCTTTGTGCCGCTTGCCACCGGAttctt > 1:4644921/1‑38 (MQ=255) ggcggcAAACGCTTTGTGCCGATTGCCACCGGAttctt > 1:4153359/1‑38 (MQ=255) ggcggcAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGTTACg < 1:2851356/70‑1 (MQ=255) gcggcAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGtt > 1:2072704/1‑66 (MQ=255) gcggcAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGtt > 1:1122535/1‑66 (MQ=255) gcAAACACTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTgg < 1:1083325/48‑1 (MQ=255) aaCGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTggc < 1:2811732/46‑1 (MQ=255) aaCGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTggc < 1:647135/46‑1 (MQ=255) gCTTTGTGCCGGTTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCAttttt < 1:4141715/53‑1 (MQ=255) gCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCAttttt < 1:4047081/53‑1 (MQ=255) | GTGGCGCAGCCTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGTTACG > W3110S.gb/704688‑704816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |