| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 976,718 | G→A | 17.5% | E216E (GAG→GAA) | mukE → | protein involved in chromosome partitioning |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 976,718 | 0 | G | A | 17.5% | 138.7 / 20.2 | 57 | E216E (GAG→GAA) | mukE | protein involved in chromosome partitioning |
| Reads supporting (aligned to +/- strand): ref base G (27/20); new base A (7/3); total (34/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GCCTGATTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCT > W3110S.gb/976651‑976782 | gCCTGATTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAAt > 1:3116275/1‑71 (MQ=255) tGATTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAg > 1:4104930/1‑71 (MQ=255) tGATTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAg > 1:3967013/1‑71 (MQ=255) gATTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAagag < 1:348773/64‑1 (MQ=255) gATTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAGc > 1:3317753/1‑71 (MQ=255) ttCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAagag < 1:989084/62‑1 (MQ=255) ttCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAagag < 1:3832826/62‑1 (MQ=255) ttCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAGc > 1:3400229/1‑69 (MQ=255) tGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAAACCGAAGAGAATCAGCCAGAt < 1:100262/71‑1 (MQ=255) tGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAAACCGAAGAGAATCAGCCAGAt < 1:2453496/71‑1 (MQ=255) caTGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAAAATCAGCCAGATAg < 1:3968258/70‑1 (MQ=255) aTGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAg < 1:2841881/70‑1 (MQ=255) ggCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAAAATCAGCCAGATAGCg > 1:5031962/1‑70 (MQ=255) gCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAAAATCAGCCAGa > 1:2837346/1‑64 (MQ=255) aGCAATGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGgaga > 1:4240271/1‑69 (MQ=255) aaTGCCGATTGAAAATCATCTGCAACTCAACGATGAAAACCGAAGAGAATCAGCCAGAt < 1:3700448/59‑1 (MQ=255) aaTGCCGATTGAAAATCATCTGCAACTCAACGATGAAAACCGAAGAGAATCAGCCAGATAGCGGAGAGgaa < 1:4193299/71‑1 (MQ=255) aaTGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGgaa < 1:1476287/70‑1 (MQ=255) gCCGATTGAAAATCATCTGCAACTCAACGATGAAAACCGAAGAGAATCAGCCAGATAGCg > 1:471639/1‑60 (MQ=255) gCCGATTGAAAATCATCTGCAACTCAACGATGAAAACCGAAGAGAATCAGCCAGATAGCGGAGAGgaagaa > 1:3550352/1‑71 (MQ=255) gCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGaaaa > 1:309676/1‑45 (MQ=255) gCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGgaagaa > 1:2782022/1‑70 (MQ=255) gCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGgaagaa > 1:3033077/1‑70 (MQ=255) cGATTGAAAATCATCTGCAACTCAACGATGAAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGaata < 1:3325893/71‑1 (MQ=255) cGATTGAAAATCATCTGCAACTCAACGATGAAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGaata < 1:1654668/71‑1 (MQ=255) gATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGaataat < 1:4525721/71‑1 (MQ=255) ttGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGa > 1:2933224/1‑71 (MQ=255) tGAAAATCATCTGCAACTCAACGATG‑AAACCGAagag > 1:3799620/1‑37 (MQ=255) tGAAAATCATCTGCAACTCAACGATG‑AAACCGAagag > 1:1023370/1‑37 (MQ=255) tGAAAATCATCTGCAACTCAACGATG‑AAACCGAagag > 1:4704804/1‑37 (MQ=255) tGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGAt > 1:2874946/1‑71 (MQ=255) tGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGAt > 1:2482775/1‑71 (MQ=255) aaTCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGaa > 1:860734/1‑71 (MQ=255) aaTCATCTGCAACTCAACGATG‑AAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGa < 1:1885533/70‑1 (MQ=255) atcatcTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAAc > 1:4785491/1‑71 (MQ=255) atcatcTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAAc > 1:2687563/1‑71 (MQ=255) tcTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCg < 1:2919911/44‑1 (MQ=255) cTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCg < 1:4372492/43‑1 (MQ=255) tGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCgg > 1:4166350/1‑43 (MQ=255) tGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGgaaga > 1:157071/1‑52 (MQ=255) cAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTaaa > 1:2199642/1‑71 (MQ=255) cAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTaaa > 1:3820526/1‑71 (MQ=255) cAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTaaa > 1:1176026/1‑71 (MQ=255) aCTCAGCGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAAtt > 1:2752152/1‑71 (MQ=255) tCAACGATGAAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTg < 1:3507937/57‑1 (MQ=255) cAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTaaa > 1:625100/1‑66 (MQ=255) cAACGATG‑AAACCGAAGAAAATCAGCCAGATAGCGGAGAgg > 1:2612029/1‑41 (MQ=255) cAACGATG‑AAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGaataa > 1:4332486/1‑49 (MQ=255) cAACGATG‑AAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGaataa > 1:3226657/1‑49 (MQ=255) aaCGATG‑AAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGaa > 1:4449011/1‑56 (MQ=255) aaCGATG‑AAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGaa > 1:1313782/1‑56 (MQ=255) aaCGATG‑AAACCGAAGAAAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTaa < 1:782459/64‑1 (MQ=255) aCGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCt < 1:2020295/71‑1 (MQ=255) aCGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCt < 1:2700204/71‑1 (MQ=255) aCGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCt < 1:5002453/71‑1 (MQ=255) tG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACt < 1:1074223/71‑1 (MQ=255) aaaCCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGa > 1:947797/1‑71 (MQ=255) aaaCCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGa > 1:3099121/1‑71 (MQ=255) aCCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGaata > 1:2382163/1‑38 (MQ=255) ccGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTa < 1:5088035/53‑1 (MQ=255) ccGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGc < 1:3020548/71‑1 (MQ=255) cGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGa > 1:2394979/1‑40 (MQ=255) cGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCg < 1:495869/59‑1 (MQ=255) cGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCt < 1:2527354/71‑1 (MQ=255) agaATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCt < 1:403884/66‑1 (MQ=255) gaATCAGCCAGATAGCGGAGAGGAAGAATAATGATTg < 1:2945192/37‑1 (MQ=255) | GCCTGATTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATG‑AAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCT > W3110S.gb/976651‑976782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |