Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,236,953	+A	coding (1005/1005 nt)	ygjR →	predicted NAD(P)‑binding dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,236,951	1	.	A	100.0%	100.7 / NA	55	*335K (TAA→AAA)	ygjR	predicted NAD(P)‑binding dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/55);  total (0/55)

GGGTGATTTTTCCGGCAGATAGCGTAAAACTAT‑AATTGCCAAAGTAAAAC  >  W3110S.gb/3236919‑3236968                                  |                  gtgTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:22541/49‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:70258/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:52551/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:52668/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:54983/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:56669/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:57794/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:58978/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:5996/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:61413/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:61792/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:6399/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:66447/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:67224/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:67446/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:48305/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:71821/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:75408/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:75410/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:76687/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:798/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:82982/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:8301/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:8341/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:85317/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:86288/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:88001/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:88570/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:32370/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:1136/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:11694/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:17477/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:18133/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:20853/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:2174/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:22954/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:23348/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:24975/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:27095/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:31361/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:1112/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:33957/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:34938/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:40776/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:40894/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:40955/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:41435/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:4237/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:42948/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:45090/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:46045/51‑1 (MQ=255) gggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:46143/51‑1 (MQ=255)  ggTGATTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:83024/50‑1 (MQ=255)      aTTTTTCCGGCAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:12885/46‑1 (MQ=255)                cAGATAGCGTAAAACTATAAATTGCCAAAGTAAAAc  <  1:35112/36‑1 (MQ=38)                                  |                  GGGTGATTTTTCCGGCAGATAGCGTAAAACTAT‑AATTGCCAAAGTAAAAC  >  W3110S.gb/3236919‑3236968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑