Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 257,873 A→G K15K (AAA→AAG)  crl → DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb257,8730AG100.0% 172.6 / NA 61K15K (AAA→AAG) crlDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (61/0);  total (61/0)

Only 100 of 109 total aligned reads displayed.

AATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTT  >  W3110S.gb/257828‑257931
                                             |                                                          
aaTGACGTTACCGAGTGGCCACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTa                                                >  1:890589/1‑58 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTa                                                >  1:561279/1‑58 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:445025/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:611781/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:608153/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:605481/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:580367/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:569/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:568196/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:53524/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:505259/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:613897/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:403123/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:364594/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:36145/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:345346/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:307438/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:268212/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:243665/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:80784/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:937120/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:912854/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:911589/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:899881/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:895941/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:868947/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:843002/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:841750/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:387699/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:804619/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:792554/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:784300/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:781823/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:758685/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:639726/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:639291/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1233480/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1354953/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1335935/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1316175/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1287014/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1256142/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1445931/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1235183/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1457629/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1206130/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1460779/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1202067/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1201893/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1470656/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1168826/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1107644/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1029398/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1022499/1‑60 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAg                                               >  1:648109/1‑59 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAg                                               >  1:1125360/1‑59 (MQ=255)
aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTATACCGCACTAgg                                              >  1:271930/1‑60 (MQ=255)
 aTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAgg                                              >  1:1429945/1‑59 (MQ=255)
                                          aaagTTTACCGCACTAGGTCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:1308944/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAgg                            >  1:1016706/1‑36 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTg                      >  1:1181014/1‑42 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:883482/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:1458235/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:327523/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:313471/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:1188863/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:839144/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:303646/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:1130876/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:274393/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:362650/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:1113684/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:179831/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:1055852/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:907760/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:2525/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:181282/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:925796/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:222218/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:1280538/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:140289/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:1418704/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:1353515/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:504808/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:57040/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:483680/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:603638/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:48112/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:433663/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:742379/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:416419/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:619140/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:1251905/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:395234/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:1016567/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:660117/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:712059/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:734923/1‑62 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt   >  1:925734/1‑61 (MQ=255)
                                          aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt   >  1:528150/1‑61 (MQ=255)
                                             |                                                          
AATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTT  >  W3110S.gb/257828‑257931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑