| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,331,211 | 0 | G | T | 73.1% | 26.0 / 20.6 | 26 | S133S (TCC→TCA) | obgE | GTPase involved in cell partioning and DNA repair |
| Reads supporting (aligned to +/- strand): ref base G (0/7); new base T (19/0); total (19/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTTTTCTGCCGCGGTGTACGGTTAACGGACGATTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGAC > W3110S.gb/3331184‑3331269 | ggTTTTCTGCCGCGGTGTACGGTTAACGGACGATTTg < 1:1303739/37‑1 (MQ=255)ggTTTTCTGCCGCGGTGTACGGTTAACGGACGATTTg < 1:135507/37‑1 (MQ=255)ggTTTTCTGCCGCGGTGTACGGTTAACGGACGATTTg < 1:670015/37‑1 (MQ=255)ggTTTTCTGCCGCGGTGTACGGTTAACGGACGATTTg < 1:440536/37‑1 (MQ=255)ggTTTTCTGCCGCGGTGTACGGTTAACGGACGATTTg < 1:3102031/37‑1 (MQ=255)ggTTTTCTGCCGCGGTGTACGGTTAACGGACGATTTg < 1:2338927/37‑1 (MQ=255)ggTTTTCTGCCGCGGTGTACGGTTAACGGACGATTTg < 1:2517563/37‑1 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAg > 1:2782800/1‑41 (MQ=38) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACcagcag > 1:415471/1‑61 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:923496/1‑62 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:1077967/1‑62 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:785633/1‑62 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:61701/1‑62 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:2874020/1‑62 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:2806966/1‑62 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:2321526/1‑62 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:1866444/1‑62 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:1840282/1‑62 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:1813013/1‑62 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:1795547/1‑62 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:1554542/1‑62 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:1468958/1‑62 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:1464486/1‑62 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:1254178/1‑62 (MQ=255) tAACTGACGACTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGa > 1:1200778/1‑62 (MQ=255) tAACTGACGACTT‑AAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGAc > 1:640348/1‑62 (MQ=255) | GGTTTTCTGCCGCGGTGTACGGTTAACGGACGATTTGAAACGGGTATTGCCCAGACCGTGCCAGCCGCCCTTAGCAACCAGCAGAC > W3110S.gb/3331184‑3331269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |