| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,269,081 | 2 bp→CC | coding (1176‑1177/1416 nt) | dnaB → | replicative DNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,269,081 | 0 | T | C | 77.8% | 31.5 / 4.3 | 18 | R392R (CGT→CGC) | dnaB | replicative DNA helicase |
| Reads supporting (aligned to +/- strand): ref base T (0/4); new base C (0/14); total (0/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | W3110S.gb | 4,269,082 | 0 | G | C | 77.8% | 33.7 / 4.3 | 18 | A393P (GCC→CCC) | dnaB | replicative DNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (0/4); new base C (0/14); total (0/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAAGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTAT > W3110S.gb/4269021‑4269128 || gAAAGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2879612/61‑1 (MQ=255) aCTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2252961/55‑1 (MQ=255) aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:949895/51‑1 (MQ=255) aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:136822/51‑1 (MQ=255) aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:137396/51‑1 (MQ=255) aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2842087/51‑1 (MQ=255) aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:1666900/51‑1 (MQ=255) aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:1794319/51‑1 (MQ=255) aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2754677/51‑1 (MQ=255) aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2503407/51‑1 (MQ=255) aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:1891975/51‑1 (MQ=255) aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2471017/51‑1 (MQ=255) aaCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2400806/51‑1 (MQ=255) aCGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGt < 1:2907715/50‑1 (MQ=255) ggCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTcc < 1:1232897/61‑1 (MQ=255) ggCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTcc < 1:218905/61‑1 (MQ=255) ggCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTcc < 1:1798107/61‑1 (MQ=255) ggCGCTGGCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTcc < 1:2718774/61‑1 (MQ=255) tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:967102/59‑1 (MQ=255) tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:1156263/59‑1 (MQ=255) tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:940666/59‑1 (MQ=255) tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:1339546/59‑1 (MQ=255) tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:29036/59‑1 (MQ=255) tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:1452226/59‑1 (MQ=255) tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:1537191/59‑1 (MQ=255) tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:2702613/59‑1 (MQ=255) tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:2657334/59‑1 (MQ=255) tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:1857772/59‑1 (MQ=255) tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:2481426/59‑1 (MQ=255) tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:2057201/59‑1 (MQ=255) tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:2059971/59‑1 (MQ=255) tcgctGGAACAACGCCCCAACAAACGTCCCGTGATGTCCGACCTGCGTGAATCCGGCGCTAt < 1:2072536/59‑1 (MQ=255) || GAAAGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTAT > W3110S.gb/4269021‑4269128 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |