Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,381,437	A→C	intergenic (+56/‑120)	ecnB → / → sugE	entericidin B membrane lipoprotein/multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,381,437	0	A	C	100.0%	24.9 / NA	9	intergenic (+56/‑120)	ecnB/sugE	entericidin B membrane lipoprotein/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/0);  total (9/0)

CTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGC  >  W3110S.gb/4381400‑4381461                                      |                         cTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACACAGGTAATAGTTGAAATTCCCCTGc  >  1:1327251/1‑62 (MQ=255) cTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACACAGGTAATAGTTGAAATTCCCCTGc  >  1:1371186/1‑62 (MQ=255) cTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACACAGGTAATAGTTGAAATTCCCCTGc  >  1:1460452/1‑62 (MQ=255) cTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACACAGGTAATAGTTGAAATTCCCCTGc  >  1:1531019/1‑62 (MQ=255) cTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACACAGGTAATAGTTGAAATTCCCCTGc  >  1:1536872/1‑62 (MQ=255) cTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACACAGGTAATAGTTGAAATTCCCCTGc  >  1:1733584/1‑62 (MQ=255) cTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACACAGGTAATAGTTGAAATTCCCCTGc  >  1:2013750/1‑62 (MQ=255) cTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACACAGGTAATAGTTGAAATTCCCCTGc  >  1:2118224/1‑62 (MQ=255) cTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACACAGGTAATAGTTGAAATTCCCCTGc  >  1:669282/1‑62 (MQ=255)                                      |                         CTGTGTCGTGCCGTTTGTTTTTTCTGCGATAGTCACAAAGGTAATAGTTGAAATTCCCCTGC  >  W3110S.gb/4381400‑4381461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑