Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	332,960	T→A	Q233L (CAG→CTG)	yahB ←	predicted DNA‑bindng transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	332,960	0	T	A	100.0%	19.5 / NA	8	Q233L (CAG→CTG)	yahB	predicted DNA‑bindng transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (8/0);  total (8/0)

CTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATT  >  W3110S.gb/332905‑332966                                                        |       cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt  >  1:1110872/1‑62 (MQ=255) cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt  >  1:1989128/1‑62 (MQ=255) cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt  >  1:2289906/1‑62 (MQ=255) cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt  >  1:2800865/1‑62 (MQ=255) cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt  >  1:2801845/1‑62 (MQ=255) cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt  >  1:302169/1‑62 (MQ=255) cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt  >  1:719359/1‑62 (MQ=255) cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACAGACAtt  >  1:857429/1‑62 (MQ=255)                                                        |       CTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATT  >  W3110S.gb/332905‑332966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑