| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,623,262 | 0 | G | A | 68.2% | 237.9 / 153.6 | 236 | E521E (GAG→GAA) | ppk | polyphosphate kinase, component of RNA degradosome |
| Reads supporting (aligned to +/- strand): ref base G (75/0); new base A (161/0); total (236/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 236 total aligned reads displayed.
CGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCA > W3110S.gb/2623216‑2623276 | cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:736823/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1490277/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1462765/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1553298/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:465222/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1346201/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1284365/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:728347/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1174181/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1161361/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1009255/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1028756/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1072084/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:878639/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:326726/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:839164/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:989600/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACgcgc > 1:655651/1‑60 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:454096/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:556449/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:512787/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:48926/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:993564/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:425226/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:380180/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:244285/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:37367/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:364860/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:333815/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:332260/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:587744/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:603294/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:603660/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:614726/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:635826/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:663124/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:670105/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:682140/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:690037/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:763560/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:793051/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:818573/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:857142/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:896039/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:95082/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1018988/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1313977/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1326094/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1256963/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1356477/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1252043/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1367911/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:222668/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1218675/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:152671/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1189692/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1152059/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1130939/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:156538/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:108033/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:174407/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:187895/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:192123/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:210294/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:217642/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:214331/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTCTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:582398/1‑61 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAATTGGTGGACCGCGAGATCGCCAACGCgca > 1:1104418/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCTAGATCGCCAACGCgca > 1:397334/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCa > 1:1119892/1‑53 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACgcgc > 1:1638348/1‑59 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:1378551/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:905892/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:978632/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:96936/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:742237/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:754311/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:1208149/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:108700/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:921270/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:1541000/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:25398/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:291313/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:298240/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:312750/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:417683/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:16861/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:446057/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:457120/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:1604758/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:700857/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:517008/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:530037/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:1509090/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:1459614/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:140494/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:628802/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:1363207/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:133933/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACAGCGAGATCGCCAACGCgca > 1:774551/1‑60 (MQ=255) | CGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCA > W3110S.gb/2623216‑2623276 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |