| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,831:1 | +G | 100% | pseudogene (251/261 nt) pseudogene (251/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,831 | 1 | . | G | 98.0% | 145.8 / ‑5.5 | 45 | pseudogene (251/261 nt) pseudogene (251/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); major base G (21/22); minor base C (0/1); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACT > W3110S.gb/547762‑547894 | cgtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g < 1:4555226/71‑1 (MQ=255) gtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg‑gga < 1:3100342/71‑3 (MQ=255) cgcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGa < 1:2399479/71‑1 (MQ=255) aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACt < 1:4445299/71‑1 (MQ=255) tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGaa > 1:2618850/1‑68 (MQ=255) gTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTg > 1:5327539/1‑65 (MQ=255) gTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACtt > 1:3586173/1‑70 (MQ=255) gTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACtt > 1:1859535/1‑70 (MQ=255) tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTc < 1:3364501/70‑1 (MQ=255) tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTc < 1:2779176/70‑1 (MQ=255) tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg‑gga < 1:3752176/58‑3 (MQ=255) tCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTcc < 1:291322/70‑1 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATAATGGAGCAGGGG‑GAAGTg > 1:2983135/1‑57 (MQ=38) tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCa > 1:4161053/1‑71 (MQ=255) gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCa < 1:403424/70‑1 (MQ=255) gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCAt > 1:1248224/1‑71 (MQ=255) aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGa < 1:1629986/46‑1 (MQ=38) aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc > 1:4826154/1‑71 (MQ=255) tGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc < 1:4210573/70‑1 (MQ=255) tGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCg < 1:3531652/71‑1 (MQ=255) aGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCg > 1:4618993/1‑71 (MQ=255) aGCGCAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGGGAACTTCATTCCTTGTCACCATGTGAATGCCGTc < 1:652005/70‑1 (MQ=255) cgcAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCggg < 1:4379555/71‑1 (MQ=255) gcAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc > 1:4355594/1‑71 (MQ=255) gcAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc > 1:5182206/1‑71 (MQ=255) gcAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc > 1:4970448/1‑71 (MQ=255) cAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTcc < 1:3281504/44‑1 (MQ=38) cAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCa < 1:609871/50‑1 (MQ=39) cAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCa < 1:4576482/50‑1 (MQ=39) cAGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc < 1:4248552/71‑1 (MQ=255) aGGCGCTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCa < 1:598339/71‑1 (MQ=255) cgcTGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATg < 1:218933/71‑1 (MQ=255) tGGCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGc > 1:1231384/1‑71 (MQ=255) gCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc < 1:1399929/62‑1 (MQ=255) gCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg > 1:941793/1‑71 (MQ=255) gCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg > 1:1040753/1‑71 (MQ=255) gCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg < 1:3594506/70‑1 (MQ=255) gCAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg < 1:1416989/70‑1 (MQ=255) cAATACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg > 1:5007988/1‑70 (MQ=255) aaTACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCAtgt > 1:3745350/1‑45 (MQ=255) aTACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt < 1:1129969/71‑1 (MQ=255) tACTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAtt < 1:3262338/71‑1 (MQ=255) tACTGGAGCACGGG‑GTAGTGAACTTCATTCCTTGTCACCATGTGAAt < 1:1165760/47‑1 (MQ=255) cTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc > 1:4765799/1‑71 (MQ=255) cTGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc > 1:5104381/1‑71 (MQ=255) tGGAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACt > 1:1608872/1‑71 (MQ=255) ggAGCAGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACt > 1:4673566/1‑70 (MQ=255) ggAGCA‑GGGTGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACt < 1:4246625/70‑1 (MQ=255) | CGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACT > W3110S.gb/547762‑547894 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |