| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,824,524 | T→G | 100% | pseudogene (657/687 nt) | rph → | ECK3633:JW3618:b3643; ribonuclease PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,824,524 | 0 | T | G | 100.0% | 287.7 / NA | 81 | pseudogene (657/687 nt) | rph | ECK3633:JW3618:b3643; ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (34/47); total (34/47) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑G‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGATTTTT > W3110S.gb/3824456‑3824588 | atcatTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGcgct > 1:1649746/1‑71 (MQ=255)atcatTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGcgct > 1:1766467/1‑71 (MQ=255) tcatTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTg > 1:3638616/1‑71 (MQ=255) tcatTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTg > 1:1686129/1‑71 (MQ=255) catTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTgg > 1:2127348/1‑71 (MQ=255) ttGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGcc > 1:808876/1‑71 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:4011340/70‑1 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc > 1:1543959/1‑70 (MQ=255) aGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g < 1:3998775/71‑1 (MQ=255) aGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g < 1:1450519/71‑1 (MQ=255) aGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g < 1:3091346/71‑1 (MQ=255) gTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑g < 1:1215560/71‑1 (MQ=255) gTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑g < 1:2937648/71‑1 (MQ=255) tGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTg < 1:4100916/62‑1 (MQ=255) tGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑gg < 1:3200147/71‑1 (MQ=255) aGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑gggga < 1:1625377/71‑3 (MQ=255) aGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑g > 1:401086/1‑67 (MQ=255) ggggACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑ggggaa > 1:3472279/1‑68 (MQ=255) gggACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑ggg < 1:388939/67‑1 (MQ=255) ggACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑GTGGg‑aatc < 1:2204984/71‑1 (MQ=255) gACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGg‑aatcg < 1:2059803/71‑1 (MQ=255) gACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGg‑aatcg < 1:4456662/71‑1 (MQ=255) ggCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g < 1:2738779/59‑1 (MQ=255) gCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGg‑aatcgaatc > 1:4274375/1‑71 (MQ=255) cAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGg‑aatcgaatc > 1:3612343/1‑70 (MQ=255) gAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCg < 1:2156009/53‑1 (MQ=255) aaGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCAtt > 1:2757799/1‑71 (MQ=255) aaGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCAtt < 1:3388543/71‑1 (MQ=255) aGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTg < 1:2909006/71‑1 (MQ=255) aGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑gggga < 1:633878/58‑3 (MQ=255) aGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑gggga < 1:4119877/58‑3 (MQ=255) gAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTg > 1:2572589/1‑42 (MQ=38) gAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGc > 1:1702098/1‑71 (MQ=255) aGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCg < 1:1448893/71‑1 (MQ=255) gCCGTTCACGCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCg > 1:1712121/1‑70 (MQ=255) gCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑gg > 1:726700/1‑49 (MQ=255) gCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑gg > 1:1947211/1‑49 (MQ=255) ttCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCg < 1:2498737/41‑1 (MQ=38) ttCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCg < 1:814858/41‑1 (MQ=38) ttCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCg < 1:349125/41‑1 (MQ=38) tCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACg > 1:2140511/1‑68 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑Aatccaatcca > 1:1904658/1‑56 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCa > 1:3302379/1‑56 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAg < 1:856093/70‑1 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAg < 1:5323230/70‑1 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAg < 1:5293998/70‑1 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGAGACGCAg < 1:2262496/70‑1 (MQ=255) aCCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAg < 1:5300224/69‑1 (MQ=255) aCCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGaa < 1:1001932/71‑1 (MQ=255) aCCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGa < 1:950119/70‑1 (MQ=255) ccATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAgg > 1:2778095/1‑71 (MQ=255) ccATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAgg < 1:4097046/71‑1 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGa > 1:2043063/1‑61 (MQ=255) aTGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCa > 1:132913/1‑51 (MQ=255) aTGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACg < 1:85282/62‑1 (MQ=255) aTGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAggcg > 1:3040977/1‑71 (MQ=255) aTGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAggcg < 1:2774330/71‑1 (MQ=255) aTGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACACAGAAggcg > 1:3598421/1‑71 (MQ=255) gAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACg < 1:2852550/60‑1 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGaa > 1:1324126/1‑64 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCAACGCAGAAGgcggcg > 1:2717052/1‑71 (MQ=255) gagCTACTCACCTTGTTGGCGCTGGCCCGA‑G‑GGGAAATCGAATCCATTGTAGc > 1:2390187/1‑53 (MQ=255) cTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTa > 1:4981970/1‑48 (MQ=255) cTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGc < 1:2534944/55‑1 (MQ=255) aCTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGaa > 1:4265161/1‑57 (MQ=255) cTCACCTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCaa < 1:4369559/70‑1 (MQ=255) accTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAggc < 1:1532321/54‑1 (MQ=255) accTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACt > 1:3446913/3‑70 (MQ=255) accTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACt < 1:4733650/68‑1 (MQ=255) ccTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGc < 1:3793334/42‑1 (MQ=37) ccTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGc < 1:4647054/42‑1 (MQ=37) ccTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGc < 1:4544717/42‑1 (MQ=37) cTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGc < 1:3439919/42‑1 (MQ=37) cTTGTTGGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGAt > 1:1539683/1‑71 (MQ=255) tgttgGCGCTGTCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACg < 1:713087/44‑1 (MQ=25) tgttgGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACg < 1:5249932/44‑1 (MQ=38) tgttgGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGAttt > 1:1303322/1‑71 (MQ=255) tgttgGCGCTGGACCGAGG‑GGG‑AATCGAATCCATTGTAGCGAc > 1:3093496/1‑43 (MQ=25) gttgGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGAtt < 1:4712786/69‑1 (MQ=255) gttgGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGAt > 1:5183624/1‑68 (MQ=255) gttgGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGAAGCGACGCAg < 1:4577174/46‑1 (MQ=37) ttgGCGCTGGCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGAttttt < 1:2921643/71‑1 (MQ=255) ggCGCTGCCCCGAGG‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGAttt > 1:4531883/1‑67 (MQ=255) | ATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑G‑GGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGATTTTT > W3110S.gb/3824456‑3824588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |