| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,303,892:1 | +C | 10.0% | intergenic (+65/‑119) | metJ → / → yiiX | DNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,303,892 | 1 | . | C | 10.0% | 226.7 / 24.0 | 60 | intergenic (+65/‑119) | metJ/yiiX | DNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase |
| Reads supporting (aligned to +/- strand): ref base . (26/28); new base C (4/2); total (30/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
AAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAA > minE/2303827‑2303961 | aaGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCa < 1:2753124/70‑1 (MQ=255) gCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAAtt > 1:2785279/1‑71 (MQ=255) aaaaaaGAGCGGCGGGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTa < 1:1439509/71‑1 (MQ=255) aaaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTAtt > 1:560992/1‑71 (MQ=255) aaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTAtt > 1:446652/1‑70 (MQ=255) aaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTg < 1:456896/71‑1 (MQ=255) agagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTg < 1:3538734/69‑1 (MQ=255) agagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTg < 1:2476149/69‑1 (MQ=255) gagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAAt < 1:3732742/71‑1 (MQ=255) cgGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTa < 1:3937039/57‑1 (MQ=255) gTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACtt < 1:4268655/71‑1 (MQ=255) tGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAAATTATTGAATTTGCACGAACTTg < 1:197399/71‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTg < 1:1978944/59‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGt < 1:1434517/70‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGt < 1:4594440/70‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGt < 1:843407/70‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGACCTACGTCATAT‑T‑GCAATTTa > 1:674553/1‑50 (MQ=255) aaTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAAt < 1:4816554/55‑1 (MQ=255) aaTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTg < 1:4278153/68‑1 (MQ=255) tCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGa < 1:1657780/51‑1 (MQ=255) tCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTACTGAATTTg > 1:327091/1‑56 (MQ=255) gCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGc > 1:4704022/1‑69 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAAtt < 1:3939240/40‑1 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTg < 1:4051071/45‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTa > 1:4396494/1‑40 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt < 1:157414/69‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt < 1:5246844/69‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt > 1:3972239/1‑69 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt < 1:2440714/69‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGa > 1:3907292/1‑70 (MQ=2) tGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGAt > 1:3803956/1‑70 (MQ=2) tGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGAt > 1:3459869/1‑70 (MQ=2) gcTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCg > 1:3102765/1‑71 (MQ=255) cTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGc < 1:3405734/59‑1 (MQ=255) tACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt > 1:5184782/1‑71 (MQ=255) tACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt > 1:4047786/1‑71 (MQ=255) aCGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt > 1:3891404/1‑70 (MQ=255) cGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGtt < 1:3029182/71‑1 (MQ=2) gCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTa > 1:3582468/1‑52 (MQ=255) cTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATa > 1:5691889/1‑60 (MQ=255) ttATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGtt < 1:303607/68‑1 (MQ=2) aTCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCAcgccg > 1:3580256/1‑71 (MQ=255) aTCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCAcgc > 1:3866421/1‑71 (MQ=2) aGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAgg > 1:4349288/1‑47 (MQ=255) aGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt < 1:3365622/53‑1 (MQ=255) aGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:5221789/71‑1 (MQ=255) ggCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATc < 1:3792219/71‑1 (MQ=255) gCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATcc > 1:3514639/1‑71 (MQ=255) gCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATc < 1:2469343/70‑1 (MQ=255) cTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt > 1:426077/1‑56 (MQ=255) cTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:5416851/71‑1 (MQ=255) aCGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGa < 1:2987731/46‑1 (MQ=255) cGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa > 1:3843765/1‑48 (MQ=255) cGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa > 1:2546639/1‑48 (MQ=255) cGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa > 1:3740576/1‑70 (MQ=255) cGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa > 1:4573581/1‑70 (MQ=255) cGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa > 1:3871214/1‑70 (MQ=255) gTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCa > 1:3675332/1‑55 (MQ=255) tCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTa > 1:2644667/1‑36 (MQ=255) tCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTa > 1:188142/1‑36 (MQ=255) atat‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaac < 1:4141506/71‑1 (MQ=255) tat‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaaca < 1:856903/71‑1 (MQ=255) at‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaa > 1:2532159/1‑71 (MQ=255) at‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaa > 1:2453031/1‑71 (MQ=255) | AAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAA > minE/2303827‑2303961 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |