| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,819 | T→C | 31.6% | Y179Y (TAT→TAC) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,819 | 0 | T | C | 31.6% | 116.2 / 78.0 | 98 | Y179Y (TAT→TAC) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base T (24/43); new base C (22/9); total (46/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.05e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 104 total aligned reads displayed.
AAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGAT > minE/835751‑835884 | aaaGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTAttt < 1:3531289/71‑1 (MQ=255)aaaGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTAttt > 1:44376/1‑71 (MQ=255) aGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGc > 1:4756403/1‑71 (MQ=255) caaCTTCGAGGCCATGCAAAAGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCgcgc > 1:594367/2‑70 (MQ=255) caaCTTCGAGGCCATGCAAAAGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGcc > 1:3923764/2‑71 (MQ=255) aCTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTAt > 1:125370/1‑59 (MQ=255) aCTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTAt > 1:1884637/1‑59 (MQ=255) cTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCcgc < 1:4191217/65‑1 (MQ=255) tCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTAt > 1:2531262/1‑56 (MQ=255) tCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCtgtg > 1:773307/1‑70 (MQ=255) tCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGc > 1:2789212/1‑71 (MQ=255) cGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCgcgc < 1:3201256/64‑1 (MQ=255) cGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGc > 1:631081/1‑70 (MQ=255) acaacaTCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGag < 1:686382/71‑2 (MQ=255) acaacaTCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGaa < 1:5528516/71‑1 (MQ=255) acaacaTCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGaa < 1:1707990/71‑1 (MQ=255) aacaTCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAaca < 1:4207407/71‑1 (MQ=255) caaaaGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCt > 1:432562/4‑71 (MQ=255) caaaaGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCt > 1:2853531/4‑71 (MQ=255) cAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGcc < 1:1291312/70‑1 (MQ=255) cAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCt > 1:5462064/1‑71 (MQ=255) cAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCt < 1:5149149/71‑1 (MQ=255) aGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCt > 1:4348567/1‑70 (MQ=255) aGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTg < 1:1781324/71‑1 (MQ=255) aGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTg < 1:3186099/71‑1 (MQ=255) aGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTg < 1:1663632/71‑1 (MQ=255) aaaGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCtca < 1:3666103/70‑3 (MQ=255) aGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCaat > 1:3079316/1‑70 (MQ=255) aGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCaat > 1:3935238/1‑70 (MQ=255) gagATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAA‑tcc < 1:5372620/69‑1 (MQ=255) gCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑ccc < 1:560082/71‑1 (MQ=255) gCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑cc < 1:691822/70‑1 (MQ=255) cGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCt > 1:3961053/1‑64 (MQ=255) gATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCtg > 1:2120516/1‑49 (MQ=255) gATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCgg < 1:5176742/71‑1 (MQ=255) gATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCgg < 1:2563743/71‑1 (MQ=255) gATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCgg < 1:5643594/71‑1 (MQ=255) gATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCgg < 1:1687524/71‑1 (MQ=255) tataGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCtg < 1:698975/45‑1 (MQ=255) aGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCAtgtgt > 1:1436486/1‑71 (MQ=255) aGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGc > 1:2746859/1‑70 (MQ=255) gTTTGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGa > 1:4997169/1‑71 (MQ=255) gTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCa < 1:2105302/61‑1 (MQ=255) gTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCa < 1:370947/61‑1 (MQ=255) gTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCg < 1:4811165/70‑1 (MQ=255) gTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCg < 1:4585217/70‑1 (MQ=255) gTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCg < 1:1012600/70‑1 (MQ=255) ttCGAAAACACCTTCATGATGTATTTGCCGCGCCtg < 1:1471726/36‑1 (MQ=255) ttCGAAAACACCTTCATGATGTATTTGCCGCGCCtg < 1:1620059/36‑1 (MQ=255) ttCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGc < 1:4370896/59‑1 (MQ=255) ttgAAAACAACTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGAcc > 1:3261578/3‑71 (MQ=255) tCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACt > 1:2540103/1‑45 (MQ=255) tCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGaa < 1:5537493/52‑1 (MQ=255) tCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGaa < 1:3212023/52‑1 (MQ=255) tCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGaa < 1:149834/52‑1 (MQ=255) tCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCAtgtg > 1:2505592/1‑63 (MQ=255) tgAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGCACACTGCCTCAATCCCAGC‑TGCGTGGCGACCt > 1:2191295/2‑71 (MQ=255) tgAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCt > 1:664389/2‑71 (MQ=255) tgAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCt > 1:727956/2‑71 (MQ=255) tgAAAACACCTTCATGATGTACATGCCGCGCCTGTGCGAACACTGCCTCaa > 1:1353259/2‑51 (MQ=255) cGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCAtgtg > 1:5626662/1‑62 (MQ=255) cGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCAtgt < 1:2833587/61‑1 (MQ=255) cGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCt > 1:139118/1‑71 (MQ=255) cGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCt > 1:83831/1‑71 (MQ=255) cGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCt > 1:4604916/1‑71 (MQ=255) gAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑cc > 1:4653343/1‑52 (MQ=255) gAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑cc > 1:2313407/1‑52 (MQ=255) gAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTTCCTCAATCCCAGC‑TGCGTGGCGACCTg > 1:2362692/1‑71 (MQ=255) gAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTg > 1:4134574/1‑71 (MQ=255) aaaaCACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGc < 1:2847320/71‑1 (MQ=255) aaaaCACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGc > 1:559860/1‑71 (MQ=255) aaaCACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCaa > 1:1943985/1‑48 (MQ=38) aaaCACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCaa > 1:5518181/1‑48 (MQ=38) aaCACCTTCATGATGTATTTGCCGCGCCTCTGCGAACACTGCCt > 1:4406981/1‑44 (MQ=255) acacCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCg < 1:3126183/71‑1 (MQ=255) acacCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCg < 1:1157043/71‑1 (MQ=255) acacCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGccca < 1:3838918/71‑2 (MQ=255) acacCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGccca < 1:3879190/71‑2 (MQ=255) cacCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGccc > 1:2062836/1‑69 (MQ=255) acCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAg < 1:2239291/71‑1 (MQ=255) acCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAg < 1:1122157/71‑1 (MQ=255) acCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAg < 1:2812730/71‑1 (MQ=255) acCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAg < 1:3395560/71‑1 (MQ=255) ccTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTg < 1:1034980/41‑1 (MQ=255) ccTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTg < 1:5573785/41‑1 (MQ=255) ccTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAg < 1:5009832/70‑1 (MQ=255) ccTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGc > 1:1982968/1‑71 (MQ=255) tCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGt > 1:4422061/1‑71 (MQ=255) tCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCggc > 1:1641382/1‑70 (MQ=255) cATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCaa > 1:3309811/1‑39 (MQ=25) cATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCggc < 1:4020327/70‑2 (MQ=255) cATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCgg < 1:860058/69‑1 (MQ=255) atgatgTACCTGCCGCGCCTGTGCGAACACTGCCTCaa > 1:2073067/1‑38 (MQ=25) atgatgTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCgg < 1:73443/68‑1 (MQ=255) atgatgTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGgcgc > 1:3207135/1‑71 (MQ=255) tgatgTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGgcgct < 1:5492776/71‑2 (MQ=255) tgatgTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCACAGC‑TGCGTGGCGACCTGCCCAAGCGgcgc > 1:3424952/1‑70 (MQ=255) atgTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGAt < 1:5454100/71‑1 (MQ=255) atgTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGAt < 1:4593942/71‑1 (MQ=255) atgTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGgcgcta < 1:1889141/70‑3 (MQ=255) | AAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGAT > minE/835751‑835884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |