| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 194,917 | G→A | 25.0% | intergenic (+113/+87) | nlpE → / ← yaeF | lipoprotein involved with copper homeostasis and adhesion/predicted lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 194,917 | 0 | G | A | 25.0% | 55.0 / 12.1 | 28 | intergenic (+113/+87) | nlpE/yaeF | lipoprotein involved with copper homeostasis and adhesion/predicted lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (6/15); new base A (4/3); total (10/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
TATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAAC‑GCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCG > minE/194849‑194985 | tatTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATgg < 1:407095/70‑1 (MQ=255) tCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATgg > 1:1185384/1‑66 (MQ=255) tCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATgg > 1:726452/1‑66 (MQ=255) cAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTc < 1:5423255/70‑1 (MQ=255) cAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTc > 1:634490/1‑70 (MQ=255) cACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAAC‑GCCTGATGCGACg < 1:4878981/70‑1 (MQ=255) cACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAAC‑GCCTGATGCGACg < 1:1848405/70‑1 (MQ=255) tttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAAC‑GCCTGATGCGACGCTGgcg > 1:823979/1‑71 (MQ=255) tttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAAC‑GCCTGATGCGACGCTGgc > 1:4768027/1‑70 (MQ=255) tccgTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACAGTGC‑CAACTGCCTGATGCGACGCTCgcgc > 1:3064589/4‑70 (MQ=11) ccgTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACAGTGC‑CAACTGCCTGATGCGACGCTTGCGCGt > 1:4934577/3‑71 (MQ=11) cgTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACAGTGC‑CAACTGCCTGATGCGACGCTTGCGCGTc > 1:3941813/2‑71 (MQ=11) tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAAC‑GCCTGATGCGACGCTGGCGCGTCtt < 1:5137867/71‑1 (MQ=255) tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAAC‑GCCTGATGCGACGCTGGCGCGTCtt < 1:1143037/71‑1 (MQ=255) ggCCGGATAAGGCGTTTACGCCGCATCCGGCAACAGTGC‑CAACTGCCTGATGCGACGCTTGCGCGTCTTAt < 1:1916953/71‑1 (MQ=11) gCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAAC‑GCCTGATGCGACGCTGGCGCGTCTTAt < 1:5507982/70‑1 (MQ=255) gCCGGATAAGGCGTTTACGCCGCATCCGGCAACAGTGC‑CAACTGCCTGATGCGACGCTTGCGCGTCTTATc < 1:4794087/71‑1 (MQ=11) ccGTATAAGGCGTTTACGCCGCATCCGGCAACAGTGC‑CAACTGCCTGATGCGACGCTTGCGCGTCTTAt < 1:1212443/69‑1 (MQ=11) gATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAAC‑GCCTGATGCGACGCTGGCGCGTCTTATCATGc > 1:883738/1‑71 (MQ=255) gCGTTTACGCCGCATCCGGCAATGGTGCTCAAC‑GCCTGATGCGACGCTg < 1:957765/49‑1 (MQ=255) gCGTTTACGCCGCATCCGGCAACAGTGC‑CAACTGCCTGATGCGACGCTTGCGCGTCTTATCAGGCCTACa > 1:1408254/1‑70 (MQ=1) gTTTACGCCGCATCCGGCAATGGTGCCGACT‑GCCTGATGCGACGCTTGCGCGTCTTATCAGGCCTACaaat < 1:5002092/71‑3 (MQ=11) ttACGCCGCATCCGGCAATGGTGCTCAAC‑GCCTGATGCGACGCTGGCGCGTCTTATCa > 1:5319103/1‑58 (MQ=255) aTCCGGCAATGGTGCTCAAC‑GCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATAcc > 1:4895975/1‑71 (MQ=255) tCCGGCAATGGTGCTCAAC‑GCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCt < 1:5325089/71‑1 (MQ=255) ggCAATGGTGCTCAAC‑GCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTaa < 1:3645414/71‑1 (MQ=255) ggCAATGGTGCTCAAC‑GCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTaa < 1:3638862/71‑1 (MQ=255) aTGGTGCTCAAC‑GCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCAt < 1:4844560/60‑1 (MQ=255) aTGGTGCTCAAC‑GCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCAt < 1:4839344/60‑1 (MQ=255) aTGGTGCTCAAC‑GCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCAt < 1:103898/60‑1 (MQ=255) aTGGTGCTCAAC‑GCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCg < 1:4997723/71‑1 (MQ=255) | TATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAAC‑GCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCG > minE/194849‑194985 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |