| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 821,576 | T→G | 34.9% | intergenic (‑113/+341) | ldrA ← / ← ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 821,576 | 0 | T | G | 34.9% | 63.2 / 62.3 | 63 | intergenic (‑113/+341) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base T (22/19); new base G (11/11); total (33/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCA > minE/821507‑821642 | aTGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGtttt < 1:1909009/71‑1 (MQ=34)aTGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttt > 1:4937720/1‑70 (MQ=35) aacaTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACctc > 1:4271205/1‑71 (MQ=34) aacaTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACctc > 1:1018866/1‑71 (MQ=34) aacaTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACctc > 1:762428/1‑71 (MQ=34) acaTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACctc < 1:5348219/70‑1 (MQ=35) caTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACctc < 1:2749988/69‑1 (MQ=35) aTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCtctc > 1:4164091/1‑70 (MQ=35) ttGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACg > 1:1730592/1‑71 (MQ=34) cGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGt < 1:753174/69‑1 (MQ=35) gCTATTCTTGAATTG‑TTCTGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACct < 1:1570890/59‑1 (MQ=11) gCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg < 1:4483300/68‑1 (MQ=35) gCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg < 1:2987968/68‑1 (MQ=35) gCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg < 1:1553419/68‑1 (MQ=35) gCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg < 1:1546760/68‑1 (MQ=35) gCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg < 1:3472529/68‑1 (MQ=35) cTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCggg > 1:1886804/1‑71 (MQ=34) cTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCgg < 1:175163/70‑1 (MQ=35) ttCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGt > 1:1870206/1‑71 (MQ=11) ttCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGt > 1:1290788/1‑71 (MQ=11) ttCTTGAATTG‑TTCAGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCaa < 1:1278158/60‑1 (MQ=11) tCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGtt > 1:5693301/1‑71 (MQ=34) cTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTAc > 1:2826786/1‑51 (MQ=35) ttGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCATGTTTTACCTCTCAACGTGCGGGGGtt < 1:3721880/69‑1 (MQ=25) ttGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttt < 1:1544248/47‑1 (MQ=38) ttGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTa < 1:3133947/49‑1 (MQ=38) ttGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGttt > 1:756040/1‑70 (MQ=35) aGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTctct < 1:459328/71‑1 (MQ=34) gtg‑tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTAc > 1:759367/1‑45 (MQ=38) tg‑tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTtct < 1:1454530/67‑1 (MQ=35) tg‑tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTtct < 1:402836/67‑1 (MQ=35) g‑tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTa > 1:2406855/1‑42 (MQ=38) g‑tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTa > 1:3040576/1‑42 (MQ=38) g‑tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTa > 1:3507434/1‑42 (MQ=38) g‑tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTa > 1:3918708/1‑42 (MQ=38) ttCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTc < 1:2163854/69‑1 (MQ=11) tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCg < 1:3780151/55‑1 (MQ=35) tGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTa > 1:4328844/1‑39 (MQ=40) tGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTa > 1:4371516/1‑39 (MQ=40) tGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTtctc > 1:3455138/1‑64 (MQ=35) gTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTAc > 1:875181/1‑38 (MQ=40) gTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCtctc < 1:3748817/43‑1 (MQ=38) gTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGc > 1:1714470/1‑70 (MQ=35) gttCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGc < 1:4008734/68‑1 (MQ=255) ttCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAAc < 1:4248633/71‑1 (MQ=21) cAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAAcc > 1:2561353/1‑70 (MQ=35) cAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAAcc < 1:848013/70‑1 (MQ=35) cAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAAcc < 1:1348151/70‑1 (MQ=35) cAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCgg > 1:616431/1‑48 (MQ=255) cAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCgg > 1:1947692/1‑48 (MQ=255) aaGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCa < 1:3345401/70‑1 (MQ=25) gATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATg > 1:2076834/1‑71 (MQ=34) gATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATg < 1:2913665/71‑1 (MQ=21) aTTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGc > 1:2321658/1‑71 (MQ=21) aTTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGc < 1:2163303/71‑1 (MQ=21) tAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGcca > 1:2765249/1‑71 (MQ=21) tAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGcca > 1:1831334/1‑71 (MQ=21) gCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGccac < 1:1062496/70‑1 (MQ=35) gTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGTGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGa > 1:4432181/1‑70 (MQ=255) ttCTGTTGTCAGGTTGTACCTCTCAACGTTCggggg < 1:4297806/36‑1 (MQ=14) ttCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGAt < 1:4036196/70‑1 (MQ=25) ttCTGTTGTAAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCtt > 1:863469/1‑45 (MQ=255) tCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAAc > 1:5281153/1‑53 (MQ=16) cTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATa > 1:559051/1‑69 (MQ=14) cTGTTGTCAGGTTGTACCTATCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGc > 1:205662/1‑58 (MQ=11) ggTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCa < 1:4729267/71‑1 (MQ=25) aggtttACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGc < 1:1827786/67‑1 (MQ=25) | ATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCA > minE/821507‑821642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |