| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,608,105:1 | +C | 20.6% | intergenic (‑215/+95) | pitA ← / ← nikR | phosphate transporter, low‑affinity/DNA‑binding transcriptional regulator, Ni‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,608,105 | 1 | . | C | 20.6% | 66.2 / 31.6 | 34 | intergenic (‑215/+95) | pitA/nikR | phosphate transporter, low‑affinity/DNA‑binding transcriptional regulator, Ni‑binding |
| Reads supporting (aligned to +/- strand): ref base . (13/14); new base C (3/4); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
TTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGCCCTTTGTTGA > minE/2608040‑2608171 | ttCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑Taa < 1:3823549/69‑1 (MQ=255) ccTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAagag < 1:3135682/70‑1 (MQ=255) ttAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAgaggag < 1:1210578/69‑1 (MQ=255) ttAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAgc < 1:563309/70‑1 (MQ=255) ttAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAgagga < 1:857298/69‑1 (MQ=255) tAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAagag > 1:1042874/1‑62 (MQ=255) atatTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAAcg < 1:2252254/71‑2 (MQ=255) tatTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAAcg < 1:1581266/70‑2 (MQ=255) ttGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAAcgtg > 1:3525982/1‑67 (MQ=255) gATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAgagg < 1:183097/57‑1 (MQ=255) cTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAACGTGGAAAggt > 1:687266/1‑70 (MQ=255) cTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAACGTGGAAAggt > 1:2967704/1‑70 (MQ=255) tGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTa > 1:419066/1‑71 (MQ=255) gTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTAt < 1:2763547/70‑1 (MQ=255) gACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTaga < 1:2232496/71‑1 (MQ=255) ccACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTaga > 1:170219/1‑69 (MQ=255) ctcTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTagagag < 1:3577709/69‑1 (MQ=255) cTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAAc < 1:810081/48‑1 (MQ=255) tGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGaa > 1:2410538/1‑71 (MQ=255) tGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGa > 1:2146907/1‑70 (MQ=255) gAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAAt > 1:2999092/1‑71 (MQ=255) gAATCTTTACAATTCACGTCCGTTTTTT‑TAAgaggag < 1:478477/37‑1 (MQ=255) gAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAg < 1:1887432/71‑1 (MQ=255) gAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAAATCGGATAGGGTATTTAGAGAGGAATCAg > 1:2107823/1‑71 (MQ=255) aaTCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAgg < 1:1628187/71‑1 (MQ=255) tttACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGa > 1:398495/1‑43 (MQ=255) ttACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAg < 1:126788/65‑1 (MQ=255) tACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAg > 1:1249411/1‑64 (MQ=255) aCAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGgt > 1:3401170/1‑65 (MQ=255) aaTTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAg > 1:516944/1‑41 (MQ=255) ttCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTgg > 1:2380378/1‑71 (MQ=255) cACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGcc > 1:3325786/1‑71 (MQ=255) aCGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTa < 1:2252844/44‑1 (MQ=255) gTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGccc < 1:2256775/65‑1 (MQ=255) ttttt‑tAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGCCCTTTGTTGa > 1:3807672/1‑71 (MQ=255) | TTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGCCCTTTGTTGA > minE/2608040‑2608171 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |