Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,225:1 +A 8.9% coding (435/2106 nt) cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,2251.A 8.9% 199.1 / 17.9 56coding (435/2106 nt)cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base . (23/28);  new base A (3/2);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

GGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTT  >  minE/385158‑385294
                                                                    |                                                                     
gggTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgc‑tc                                                                     <  1:3735801/70‑1 (MQ=255)
  gtggtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTcgc‑tcgct                                                                  <  1:2587752/71‑1 (MQ=255)
      ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTg                                                                 <  1:2357919/68‑1 (MQ=255)
      ttCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                                              <  1:1984576/71‑1 (MQ=255)
        ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTggg                                                               >  1:223380/1‑68 (MQ=255)
        ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                                              >  1:1782952/1‑69 (MQ=255)
        ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                                              >  1:2163028/1‑69 (MQ=255)
        ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGt                                                              >  1:1024244/1‑69 (MQ=255)
        ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTg                                                             <  1:1044657/70‑1 (MQ=255)
           gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGa                                                            <  1:3016120/68‑1 (MQ=255)
           gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGa                                                            <  1:1268447/68‑1 (MQ=255)
           gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGa                                                            <  1:1010090/68‑1 (MQ=255)
            ccGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTg                                                        >  1:1857168/1‑71 (MQ=255)
             cggtgcggtgCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTgg                                                       <  1:3503223/71‑1 (MQ=255)
                    gtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgc‑tc                                                                     <  1:73588/50‑1 (MQ=255)
                      gcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAaag                                                 >  1:3469037/1‑68 (MQ=255)
                       cAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAagaaga                                             <  1:3604802/71‑1 (MQ=255)
                       cAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAaagaa                                               >  1:2566236/1‑69 (MQ=255)
                           aTTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                         >  1:1667077/1‑71 (MQ=255)
                            tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGTGCTGGTCAAAGAAGAGATgg                                        >  1:2983340/1‑71 (MQ=255)
                            tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                        <  1:2382705/71‑1 (MQ=255)
                            tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                        <  1:3428842/71‑1 (MQ=255)
                            tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                        <  1:150903/71‑1 (MQ=255)
                            tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                        <  1:1753496/71‑1 (MQ=255)
                               taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                                     >  1:1700810/2‑69 (MQ=255)
                               taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac                                     >  1:3188782/2‑69 (MQ=255)
                               taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATggg                                       <  1:122186/68‑1 (MQ=255)
                               cATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGc                                     >  1:724233/1‑71 (MQ=255)
                                 tGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATGGGAcc                                    <  1:766422/70‑1 (MQ=255)
                                    tGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                        <  1:2645659/63‑1 (MQ=255)
                                    tGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                         <  1:38885/62‑1 (MQ=255)
                                      cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCTCTGGGTGAGCTGGTCAAAGAAGAGATg                                         <  1:3191507/60‑1 (MQ=255)
                                      cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                         <  1:3880871/60‑1 (MQ=255)
                                      cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgc                              >  1:1328731/1‑71 (MQ=255)
                                       tgtttgttTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAagaaga                                             >  1:3225604/1‑55 (MQ=255)
                                        gtttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg                            >  1:2727909/1‑71 (MQ=255)
                                         tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAgaga                                           >  1:737161/1‑55 (MQ=255)
                                         tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg                                        <  1:2236217/58‑1 (MQ=255)
                                         tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgcc                             <  1:3240845/69‑1 (MQ=255)
                                         tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgg                           <  1:1378872/71‑1 (MQ=255)
                                           tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                                       >  1:2850384/1‑57 (MQ=255)
                                           tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATggg                                       >  1:3492533/1‑57 (MQ=255)
                                           tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg                         >  1:3356221/1‑71 (MQ=255)
                                           tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg                         >  1:1070097/1‑71 (MQ=255)
                                             tttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGt                        <  1:3695223/70‑1 (MQ=255)
                                               tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGAt                                          <  1:729648/50‑1 (MQ=255)
                                               tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCa                                   >  1:2277568/1‑57 (MQ=255)
                                               tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGa                      <  1:2329850/70‑1 (MQ=255)
                                                cTACGCGCCGTGACGGTCGC‑TCGCTTGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                    >  1:1979833/1‑71 (MQ=255)
                                                cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                    >  1:2331255/1‑71 (MQ=255)
                                                cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                    >  1:1375815/1‑71 (MQ=255)
                                                cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAt                     <  1:3189982/70‑1 (MQ=255)
                                                  aCGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg                                         <  1:851634/48‑1 (MQ=255)
                                                   cgcgCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc                  <  1:548553/70‑1 (MQ=255)
                                                   cgcgCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTgc                  <  1:1671666/70‑1 (MQ=255)
                                                       ccGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCtgg             <  1:1977237/71‑1 (MQ=255)
                                                           gACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAgaga                                           >  1:2444047/1‑37 (MQ=255)
                                                           gACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGAt                                          <  1:1411758/38‑1 (MQ=255)
                                                            aCGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGcc        >  1:383128/1‑71 (MQ=255)
                                                                tcgc‑tcgcTGGGTGAGCTGGTCAAAGAAGAGATgggg                                      >  1:3600125/1‑37 (MQ=255)
                                                                tcgc‑tcgcTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCt    <  1:2502337/71‑1 (MQ=255)
                                                                tcgc‑tcgcTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCt    >  1:1313037/1‑71 (MQ=255)
                                                                  gc‑tcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCttt  <  1:1779906/71‑1 (MQ=255)
                                                                  gc‑tcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCttt  <  1:1056735/71‑1 (MQ=255)
                                                                   c‑tcgctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGAtt                    <  1:1563325/52‑1 (MQ=255)
                                                                    |                                                                     
GGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTT  >  minE/385158‑385294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑