| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,211,310 | A→G | 20.5% | S14S (TCT→TCC) | cspC ← | stress protein, member of the CspA‑family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,211,310 | 0 | A | G | 20.5% | 171.8 / 38.2 | 83 | S14S (TCT→TCC) | cspC | stress protein, member of the CspA‑family |
| Reads supporting (aligned to +/- strand): ref base A (28/38); new base G (11/6); total (39/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
CCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTC > minE/1211240‑1211379 | ccTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa < 1:1762428/71‑1 (MQ=255) gATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGAc < 1:831281/70‑1 (MQ=255) gATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGAc < 1:2987128/70‑1 (MQ=255) gATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGACGGAGTAATGAAGCCAAAACCTTTAGAc < 1:3585649/70‑1 (MQ=255) gATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGACGGAGTAATGAAGCCAAAACCTTTAGAc < 1:3581191/70‑1 (MQ=255) aTAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTc > 1:95075/1‑71 (MQ=255) aTAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTc > 1:855873/1‑71 (MQ=255) aTAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTc > 1:1876307/1‑71 (MQ=255) gCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGtt > 1:74359/1‑71 (MQ=255) cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTg < 1:2159560/71‑1 (MQ=255) agaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAAc > 1:2135013/1‑71 (MQ=255) agaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAc > 1:3516745/1‑71 (MQ=255) agaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAc > 1:3304559/1‑71 (MQ=255) agaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAc > 1:1887077/1‑71 (MQ=255) gaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAAcc < 1:699068/71‑1 (MQ=255) aGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCAc < 1:1681386/71‑1 (MQ=255) tgtACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACtt < 1:3909223/71‑1 (MQ=12) gtACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCTAAACCTTTAGACTCGTTGAACCACtt > 1:868425/1‑70 (MQ=255) gtACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACtt > 1:845793/1‑70 (MQ=255) tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTaaa > 1:578390/1‑63 (MQ=9) tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTa < 1:1194781/70‑1 (MQ=255) aCGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAAc > 1:530704/1‑71 (MQ=255) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAcg > 1:1793018/1‑70 (MQ=255) aaCACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGa > 1:1510217/1‑50 (MQ=255) acacATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTc < 1:272189/52‑1 (MQ=255) cacaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGAc > 1:3287333/1‑71 (MQ=255) cacaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGAc < 1:2904577/71‑1 (MQ=255) acaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCt < 1:2200473/67‑1 (MQ=255) aaaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGAc > 1:263882/3‑70 (MQ=255) gtCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCtt > 1:1462865/2‑71 (MQ=255) aTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCtt < 1:1820088/71‑1 (MQ=255) cTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCtt > 1:2838813/1‑69 (MQ=255) cTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGaa > 1:1231530/1‑52 (MQ=255) cTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCttt > 1:3855000/1‑70 (MQ=255) tgctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTa > 1:3592555/1‑56 (MQ=255) tgctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTa > 1:111644/1‑56 (MQ=255) tgctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATc > 1:1210538/1‑71 (MQ=255) tgctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATc > 1:2793454/1‑71 (MQ=255) tgctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATc > 1:2031568/1‑71 (MQ=255) ctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCtt < 1:1277899/71‑1 (MQ=255) ctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCtt < 1:501487/71‑1 (MQ=255) ctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCtt < 1:83884/71‑1 (MQ=255) tgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTg < 1:1698098/44‑1 (MQ=255) cATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGcc < 1:3587172/71‑1 (MQ=255) aTCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGa > 1:2382057/1‑55 (MQ=255) aTCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCa < 1:3058124/71‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAcc < 1:998981/43‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAcc < 1:690369/43‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt < 1:1016430/71‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt < 1:2182732/71‑1 (MQ=255) ttccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtc > 1:2289156/3‑69 (MQ=255) aGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAttt > 1:2278971/1‑71 (MQ=255) aGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAttt < 1:3669882/71‑1 (MQ=255) aGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAtt < 1:992184/70‑1 (MQ=255) tccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCtt < 1:2279505/54‑1 (MQ=16) gCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTg < 1:3357793/71‑1 (MQ=255) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTaa < 1:131685/57‑1 (MQ=255) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTaa < 1:1532320/57‑1 (MQ=255) gAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt < 1:1934341/64‑1 (MQ=255) aGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGAc < 1:2600584/47‑1 (MQ=255) aGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaaa < 1:1373721/71‑1 (MQ=255) gTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGATAAAt > 1:2332611/1‑71 (MQ=255) tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTg < 1:174917/43‑1 (MQ=255) tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAAtt < 1:354049/71‑1 (MQ=255) tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAAt < 1:1242601/70‑1 (MQ=255) aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAttt > 1:3767160/1‑61 (MQ=255) aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc > 1:901757/1‑71 (MQ=255) aGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAg < 1:1061082/71‑1 (MQ=255) gCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGa < 1:1836371/71‑1 (MQ=255) cAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGact > 1:1847833/1‑69 (MQ=255) cAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGAt > 1:446508/1‑70 (MQ=255) aaaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAttt > 1:3183931/1‑53 (MQ=255) aaaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTg < 1:1081798/71‑1 (MQ=255) aaaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTg > 1:3707406/1‑71 (MQ=255) aaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCt < 1:1876481/63‑1 (MQ=255) aCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGttt > 1:582833/1‑71 (MQ=255) ccTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCttt > 1:3533376/1‑42 (MQ=255) ccTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGtttt < 1:3845560/71‑1 (MQ=255) ccTTTAGAATCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGtttt < 1:42467/71‑1 (MQ=255) cTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGtttt < 1:2472973/70‑1 (MQ=255) ttAGACTCGTTGAAGCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTttct > 1:3740998/1‑70 (MQ=255) ttAGACTCGTTGAACCACTTAACCTGACCTTTAATCt < 1:1715342/37‑1 (MQ=255) ttAGACTCGTTGAACCACTTAACCTGACCTTTAATCt < 1:2574013/37‑1 (MQ=255) ttAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTttct > 1:3428869/1‑70 (MQ=255) aGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTttcttc < 1:724211/70‑1 (MQ=255) | CCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTC > minE/1211240‑1211379 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |